FastQCFastQC Report
Mon 6 Feb 2017
SRR2077133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178360
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT550.004491611419.99505636
GTATCAA52850.018.6933671
GGTATCA30800.017.0739271
CGTGCCG2700.015.48545310
TATCAAC67200.014.4402942
TGTGCGA2501.382432E-1014.08361110
TCAACGC68900.013.9564774
ATCAACG69750.013.9439173
TGGACCG3500.013.8319025
CAACGCA69850.013.8301025
GCACCGT4650.013.7226536
AACGCAG71700.013.5335736
GTACATG75450.013.0648661
TACATGG73600.013.0351312
GTACTAT4100.012.8799331
TAGGTCG1200.00195931312.8364235
GTAGGAC4300.012.7931743
GACCGTA1557.1641975E-512.7770687
ACCGGTC1609.639797E-512.3779798
GTATAGG5700.012.3526841
Produced by FastQC (version 0.11.5)