FastQCFastQC Report
Mon 6 Feb 2017
SRR2077132_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077132_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544115
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.023.4888081
GTATCAA43600.021.6045971
TATCAAC57750.016.4246372
ATCAACG57300.016.1702353
TCAACGC57300.016.0163734
CAACGCA58900.015.6173345
CGCTAGC850.00297610715.533624
AACGCAG61800.014.9495746
CGTATAC1351.9510184E-514.672283
ACCGTCG1351.9604113E-514.6648228
GTACATG96350.014.3220341
ATCGTCG2052.9534931E-813.94355744
TACATGG99700.013.8402152
ACATGGG100500.013.2269513
GTGTAGG7200.012.8382451
ACGCAGA71750.012.7536167
TATACCG1250.002661316412.3234975
CGCCTTA2706.5647328E-912.21930533
GTCTTAG8150.012.1518891
GTGGTAT11450.012.1094361
Produced by FastQC (version 0.11.5)