##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077132_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3544115
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.391743213750118	31.0	30.0	34.0	25.0	34.0
2	29.502038449655274	31.0	30.0	34.0	24.0	34.0
3	29.58055791079014	31.0	30.0	34.0	25.0	34.0
4	32.97829133648315	37.0	35.0	37.0	26.0	37.0
5	32.949385389582446	37.0	35.0	37.0	26.0	37.0
6	33.06889166971162	37.0	35.0	37.0	26.0	37.0
7	32.9486740695491	37.0	35.0	37.0	26.0	37.0
8	32.88632084455499	37.0	35.0	37.0	25.0	37.0
9	34.293414858152175	39.0	35.0	39.0	25.0	39.0
10	34.23333469709645	39.0	35.0	39.0	25.0	39.0
11	34.273206145963094	39.0	35.0	39.0	25.0	39.0
12	34.17506345025486	39.0	35.0	39.0	25.0	39.0
13	34.12391443279916	39.0	35.0	39.0	25.0	39.0
14	35.113375553558505	40.0	35.0	41.0	24.0	41.0
15	35.15589759361646	40.0	35.0	41.0	25.0	41.0
16	35.13077369103429	40.0	35.0	41.0	25.0	41.0
17	34.98227145563843	39.0	35.0	41.0	23.0	41.0
18	34.95219427134842	39.0	35.0	41.0	23.0	41.0
19	35.07225386309417	40.0	35.0	41.0	24.0	41.0
20	35.05934655054929	39.0	35.0	41.0	24.0	41.0
21	35.01595856793586	39.0	35.0	41.0	23.0	41.0
22	34.952591831811326	39.0	35.0	41.0	23.0	41.0
23	34.92225816600195	39.0	35.0	41.0	23.0	41.0
24	34.922641900728394	39.0	35.0	41.0	23.0	41.0
25	34.83044737543787	39.0	34.0	41.0	22.0	41.0
26	34.79318447623737	39.0	34.0	41.0	22.0	41.0
27	34.73724780375355	39.0	34.0	41.0	22.0	41.0
28	34.64214281985771	39.0	34.0	41.0	20.0	41.0
29	34.6296841383533	39.0	34.0	41.0	21.0	41.0
30	34.47378569826318	39.0	34.0	41.0	19.0	41.0
31	34.47097935591819	39.0	34.0	41.0	19.0	41.0
32	34.35431863808031	39.0	34.0	41.0	18.0	41.0
33	34.31495338046311	39.0	34.0	41.0	18.0	41.0
34	34.23865647700484	39.0	34.0	41.0	18.0	41.0
35	34.17718132735535	39.0	34.0	41.0	16.0	41.0
36	34.091100599162274	39.0	34.0	41.0	16.0	41.0
37	34.00587480936708	39.0	33.0	41.0	16.0	41.0
38	33.91940244602672	39.0	33.0	41.0	15.0	41.0
39	33.82391542035177	39.0	33.0	41.0	15.0	41.0
40	33.756036415296904	38.0	33.0	41.0	13.0	41.0
41	33.65781274027508	38.0	33.0	40.0	9.0	41.0
42	33.51345145403013	38.0	33.0	40.0	9.0	41.0
43	33.43509056562781	38.0	33.0	40.0	8.0	41.0
44	33.307253573882335	38.0	33.0	40.0	7.0	41.0
45	33.171954352497025	38.0	33.0	40.0	2.0	41.0
46	33.0477157202856	38.0	33.0	40.0	2.0	41.0
47	32.900365535542726	38.0	33.0	40.0	2.0	41.0
48	32.74077562381582	38.0	32.0	40.0	2.0	41.0
49	32.579925594965175	38.0	32.0	40.0	2.0	41.0
50	32.38509952414072	38.0	32.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.21128779155550248
1101	2	0.371106599146831
1101	3	0.486785608295655
1101	4	0.432393118678867
1101	5	0.5188710880786829
1101	6	0.5709115724244214
1101	7	0.7105229556839632
1101	8	0.706732412287316
1101	9	0.9581312148466452
1101	10	0.9533554173269323
1101	11	0.7445097543560522
1101	12	0.9131372332505236
1101	13	0.9459311172383735
1101	14	1.1843695439279784
1101	15	1.200117314808061
1101	16	1.201338134435872
1101	17	1.245273498004046
1101	18	1.1956026515821279
1101	19	1.3143247672655747
1101	20	1.246119059599792
1101	21	1.250741773105844
1101	22	1.255949988389574
1101	23	1.461254752130614
1101	24	1.3454872149585526
1101	25	1.2567900064104904
1101	26	1.314915578485362
1101	27	1.2911900355820336
1101	28	1.3280580227647292
1101	29	1.3400319112744512
1101	30	1.480865698032467
1101	31	1.5326410149362317
1101	32	1.439922924824522
1101	33	1.4657359425675907
1101	34	1.6970132319999394
1101	35	1.6338353509208474
1101	36	1.731013880687442
1101	37	1.6420466230926323
1101	38	1.5616629940949878
1101	39	1.5367436354136572
1101	40	1.4260249590862628
1101	41	1.5930898588818252
1101	42	1.481858427342516
1101	43	1.7076185981218899
1101	44	1.6138487810833837
1101	45	1.5970808865350037
1101	46	1.5434874495149131
1101	47	1.6266737558890085
1101	48	1.5521525169548909
1101	49	1.591909833726838
1101	50	1.732136961348175
1102	1	0.6065773416469078
1102	2	0.6568238646162641
1102	3	0.6783573774862042
1102	4	0.8069819786854708
1102	5	0.7708512346985259
1102	6	0.8459271466113165
1102	7	0.9895053123550923
1102	8	1.0375355582730705
1102	9	1.1980246799919883
1102	10	1.2332328262272156
1102	11	1.1733547244499078
1102	12	1.0946469652632658
1102	13	1.1966328826837014
1102	14	1.4015886616132107
1102	15	1.2570729506242415
1102	16	1.2676915868993746
1102	17	1.460107818320573
1102	18	1.475867561084975
1102	19	1.3966908941506944
1102	20	1.3833744614323535
1102	21	1.3130204435152564
1102	22	1.3355628215195807
1102	23	1.2956306907534838
1102	24	1.3218737811099075
1102	25	1.4707121243515857
1102	26	1.4029398630925058
1102	27	1.2816387946530696
1102	28	1.4879395662408115
1102	29	1.3518576324149265
1102	30	1.3849379770922283
1102	31	1.5450651371268478
1102	32	1.5444312811174186
1102	33	1.4596791598237928
1102	34	1.5816483695904608
1102	35	1.5554086589893927
1102	36	1.562387153157843
1102	37	1.593688309257935
1102	38	1.600845979666616
1102	39	1.607449980411566
1102	40	1.5468713790627575
1102	41	1.5259396198922204
1102	42	1.523445678016472
1102	43	1.3700629296740843
1102	44	1.3789631586535833
1102	45	1.4205850229648789
1102	46	1.4698410132226059
1102	47	1.501447559365019
1102	48	1.4609012237411534
1102	49	1.5072655184904633
1102	50	1.4397251131000814
1103	1	0.2384277618319146
1103	2	0.34904119196845684
1103	3	0.2985601691390123
1103	4	0.38163029097135137
1103	5	0.3951105421857761
1103	6	0.48972362633654143
1103	7	0.41111102642900477
1103	8	0.5322468714865494
1103	9	0.5875737504543821
1103	10	0.5436528519191839
1103	11	0.5526597228096151
1103	12	0.640916004616976
1103	13	0.5557655624356528
1103	14	0.7152070971470721
1103	15	0.673407453784364
1103	16	0.4982320806911318
1103	17	0.7151256290797932
1103	18	0.5674812067666153
1103	19	0.643996439708431
1103	20	0.5934198613112116
1103	21	0.4118525593898852
1103	22	0.5263643186897866
1103	23	0.575944502155167
1103	24	0.5087280069710118
1103	25	0.5280384735133481
1103	26	0.651682047313237
1103	27	0.5782778348098603
1103	28	0.6423614578682972
1103	29	0.6246193063791878
1103	30	0.7739097747726689
1103	31	0.6389666590396459
1103	32	0.6212000263887774
1103	33	0.6920478331108058
1103	34	0.5946626113700546
1103	35	0.585208070109374
1103	36	0.5835821354527511
1103	37	0.6742523670287781
1103	38	0.5953248231312429
1103	39	0.6145406735258092
1103	40	0.6899826826523139
1103	41	0.6545399748068732
1103	42	0.6317226496281805
1103	43	0.6064651563116072
1103	44	0.5959204287629518
1103	45	0.5911710401858485
1103	46	0.6103216696261384
1103	47	0.5940654174635327
1103	48	0.5578479774392946
1103	49	0.555480191460795
1103	50	0.4695973945451897
1104	1	0.6181460715459224
1104	2	0.6801299133173622
1104	3	0.6735112668118859
1104	4	0.6403092705258331
1104	5	0.7314742417870761
1104	6	0.6903333386832955
1104	7	0.6368652945056041
1104	8	0.7420456161089035
1104	9	0.8012835862984318
1104	10	0.6566400282701537
1104	11	0.7805839853586534
1104	12	0.7564732502575708
1104	13	0.7398528901084873
1104	14	0.9350589493424408
1104	15	0.8448087094711738
1104	16	0.891196786124901
1104	17	0.9467058598768787
1104	18	0.8309648520378587
1104	19	0.8570348467620619
1104	20	0.8260309538081998
1104	21	0.7471728053848565
1104	22	0.8003507088678177
1104	23	0.8383947737860993
1104	24	0.81084284824923
1104	25	0.7562956325757213
1104	26	0.7702628963052973
1104	27	0.8660873042584214
1104	28	0.8128003827423385
1104	29	0.8333420709873138
1104	30	0.8483309045455627
1104	31	0.8606635636720839
1104	32	0.8837802748338532
1104	33	0.8799191091752476
1104	34	0.927034064751723
1104	35	0.922309829330743
1104	36	0.9201746792664665
1104	37	0.9258726652076987
1104	38	0.9319089616058847
1104	39	0.9011338807494269
1104	40	0.9446102565700301
1104	41	1.0416496369792299
1104	42	0.9031192936500219
1104	43	0.9257807027881029
1104	44	1.0014518246044375
1104	45	1.006348963501587
1104	46	0.9410227792468362
1104	47	1.0011839731931218
1104	48	1.0906732740447111
1104	49	1.1835243572988432
1104	50	1.1321658304294573
1105	1	0.5709822780767126
1105	2	0.5185540707070366
1105	3	0.4506186399783729
1105	4	0.515869681800524
1105	5	0.5761235790472483
1105	6	0.5056313411734195
1105	7	0.4921629946308883
1105	8	0.5105014535302601
1105	9	0.5890989418477801
1105	10	0.5800790576205159
1105	11	0.5951218085171703
1105	12	0.5737495478294363
1105	13	0.677177780635347
1105	14	0.751357681897332
1105	15	0.7117840321115239
1105	16	0.6883125426002579
1105	17	0.8049619804482688
1105	18	0.728212746822706
1105	19	0.571681570029476
1105	20	0.6582020113487133
1105	21	0.6269514499418207
1105	22	0.6507411984208673
1105	23	0.6565945033158727
1105	24	0.7715690700591935
1105	25	0.7302487309130612
1105	26	0.7127070185523792
1105	27	0.7201759108100774
1105	28	0.7013076173086858
1105	29	0.6878332616853982
1105	30	0.711634567110309
1105	31	0.657999631944115
1105	32	0.7870587630877068
1105	33	0.8408084616902158
1105	34	0.769446633765277
1105	35	0.7355317558531596
1105	36	0.693731094448502
1105	37	0.7579336278105373
1105	38	0.7652225436879405
1105	39	0.7474248883704888
1105	40	0.732852824369246
1105	41	0.7917246185812985
1105	42	0.8281131853926809
1105	43	0.7992657696229273
1105	44	0.8242739565398693
1105	45	0.9045384565186723
1105	46	0.9524710677887214
1105	47	0.8079118833693073
1105	48	0.8663664820039259
1105	49	0.8109584551762907
1105	50	0.8756684002867061
1106	1	0.5535725949713459
1106	2	0.46579102137099326
1106	3	0.4629500387045624
1106	4	0.4896294646761419
1106	5	0.4765518715248902
1106	6	0.4445252094709886
1106	7	0.49981295755844
1106	8	0.5325779666965644
1106	9	0.6664018866969883
1106	10	0.5649008462462746
1106	11	0.5287261435951152
1106	12	0.5989721131479584
1106	13	0.6414596271995805
1106	14	0.7530359167832401
1106	15	0.7175803725548775
1106	16	0.6176609082063393
1106	17	0.8116854883712321
1106	18	0.5118855032507739
1106	19	0.717289019098196
1106	20	0.6527886262231988
1106	21	0.7217438750975091
1106	22	0.7722901475914767
1106	23	0.7909047111353331
1106	24	0.6866906159209734
1106	25	0.7999040877514147
1106	26	0.772396393697548
1106	27	0.8212432221241386
1106	28	0.7391479963073948
1106	29	0.7277177314876226
1106	30	0.8443553619284287
1106	31	0.7206276102677762
1106	32	0.7954374692045931
1106	33	0.7312776597330952
1106	34	0.7798862925942487
1106	35	0.785703533578058
1106	36	0.7653890997939641
1106	37	0.8282413742663266
1106	38	0.9268904856848792
1106	39	0.8869592004544913
1106	40	0.9762942544110871
1106	41	0.8903664274698571
1106	42	0.8338350721975019
1106	43	0.8306402004147628
1106	44	0.8676936085955731
1106	45	0.8426883821600271
1106	46	0.7857874204676492
1106	47	0.8363019938361802
1106	48	0.839453925961827
1106	49	0.7907809254031477
1106	50	0.9077194448463217
1107	1	0.1257816414465296
1107	2	0.021203744510231104
1107	3	-0.002887850246466428
1107	4	-0.013906411316852996
1107	5	-0.04940554392607055
1107	6	-0.005112855846121533
1107	7	0.04693590849394269
1107	8	0.0042486248204554045
1107	9	0.07423157372602418
1107	10	-0.0287588620454855
1107	11	-0.04070588451788382
1107	12	-0.09059304554706671
1107	13	0.07570510397950869
1107	14	-0.0497609196983575
1107	15	0.014439568495120625
1107	16	-0.006421773875409542
1107	17	0.004567758815340994
1107	18	0.1543497538508305
1107	19	0.008282279419411509
1107	20	-0.005317311610035347
1107	21	-0.049129351884424466
1107	22	-0.30251455284135176
1107	23	-0.3230311405200297
1107	24	-0.24194476816924748
1107	25	-0.16596617935876168
1107	26	0.1215359229512245
1107	27	-0.007427821734864892
1107	28	-0.05600800335673739
1107	29	0.05352396516789071
1107	30	0.03699924854075931
1107	31	-0.01315069843266059
1107	32	0.027493458369910684
1107	33	-0.00730639694602786
1107	34	-0.02280447549043174
1107	35	0.13222370187207844
1107	36	-0.01782000145257001
1107	37	-0.02540187225694268
1107	38	0.026672877595252942
1107	39	0.1083580163422937
1107	40	0.1226957650726419
1107	41	0.17534336452752797
1107	42	0.061311756127459205
1107	43	0.027381223373069474
1107	44	0.2447659421622035
1107	45	0.2042332717186781
1107	46	0.030382340792854734
1107	47	0.12435850548980909
1107	48	0.018130563739042316
1107	49	-0.04951697234633201
1107	50	0.05656814874481597
1108	1	0.2679473012612483
1108	2	0.19536714081279172
1108	3	0.22087905048268652
1108	4	0.17212443561625435
1108	5	0.20848811994837746
1108	6	0.1198303197016628
1108	7	0.043698934886343466
1108	8	0.16952151956252237
1108	9	0.045639206314866954
1108	10	0.07520798110309812
1108	11	0.13445945761091593
1108	12	0.02058181630804512
1108	13	0.10529973467193798
1108	14	0.012496400353121828
1108	15	0.07828667452879756
1108	16	0.09493668550489076
1108	17	-0.005845661152051207
1108	18	0.0644145677806236
1108	19	0.040783528460700325
1108	20	0.027881963345741667
1108	21	0.0540335508044123
1108	22	-0.11637782022253873
1108	23	-0.1359239767026068
1108	24	-0.027863218702364634
1108	25	0.13242083735345744
1108	26	0.0532013590467173
1108	27	0.021236117358093054
1108	28	-0.10290771434566892
1108	29	-0.011541417872201976
1108	30	-0.06122670879974379
1108	31	0.0018491099399327027
1108	32	0.13832102441094207
1108	33	0.09648979968862648
1108	34	0.12283578745930868
1108	35	0.13907683470311838
1108	36	-0.11032683308503977
1108	37	-0.02235909455535534
1108	38	-0.009534976496404113
1108	39	0.03129635364439309
1108	40	0.007421952596907033
1108	41	0.09493249343551469
1108	42	0.22614806361048778
1108	43	0.22826876417470032
1108	44	0.14928776021221069
1108	45	0.13005736191562534
1108	46	0.11407474463629796
1108	47	0.05438243929108211
1108	48	0.0863970191076362
1108	49	0.07730383432654975
1108	50	0.10561469273299906
1109	1	-0.0704517699423306
1109	2	-0.09101538802015341
1109	3	-0.12522938380226734
1109	4	-0.09050229861433223
1109	5	-0.16862169632589996
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2210	44	-0.168343970092927
2210	45	0.008168993234704658
2210	46	-0.014773466370314736
2210	47	0.061703187346488164
2210	48	-0.0349172203950161
2210	49	0.14680780961300854
2210	50	0.09979206121334272
2211	1	-0.034249795516185344
2211	2	-0.0565765579409252
2211	3	-0.14166871249073765
2211	4	-0.1464216453342857
2211	5	-0.31457789580097995
2211	6	-0.14278411075937214
2211	7	-0.14397676991681863
2211	8	-0.19523064886963226
2211	9	-0.1558212301171622
2211	10	-0.14963265315817864
2211	11	-0.11602991618218539
2211	12	-0.12084031168805609
2211	13	-0.186890226734576
2211	14	-0.3345692724241047
2211	15	-0.23713714005098296
2211	16	-0.2642152771439825
2211	17	-0.10754504927518127
2211	18	-0.2508257316267475
2211	19	-0.24462064731616806
2211	20	-0.30360253586479047
2211	21	-0.21725751577505292
2211	22	-0.20327695768412468
2211	23	-0.31069911788215876
2211	24	-0.1881656886489722
2211	25	-0.25396725111510676
2211	26	-0.28101084036238433
2211	27	-0.12742143409456475
2211	28	-0.05715913207021117
2211	29	-0.17259835808651047
2211	30	-0.4553679284852521
2211	31	-0.33485191779063683
2211	32	-0.14126744780329403
2211	33	-0.30982921090976134
2211	34	-0.2177278384310739
2211	35	-0.09570029954583958
2211	36	-0.07720214492970712
2211	37	-0.011152639929385089
2211	38	0.01174622735400277
2211	39	-0.002383725877713516
2211	40	0.02355122025107903
2211	41	0.00851429228784184
2211	42	-0.03321086812255203
2211	43	-0.14354283786087763
2211	44	-0.2409884098837196
2211	45	-0.17126907822525084
2211	46	-0.18591925791227482
2211	47	-0.2305105848747715
2211	48	-0.19652397233721786
2211	49	-0.11572482391358108
2211	50	-0.036418012304586966
2212	1	-0.2072889050283102
2212	2	-0.28396480885111686
2212	3	-0.2597925613693839
2212	4	-0.19764484364753088
2212	5	-0.2150518631855931
2212	6	-0.35708072017912684
2212	7	-0.23963143051700797
2212	8	-0.12708675052319762
2212	9	-0.34641308656455294
2212	10	-0.3986624656829463
2212	11	-0.35314687615635165
2212	12	-0.4382432723204843
2212	13	-0.3112671481419085
2212	14	-1.0448624285221797
2212	15	-0.6302405976774565
2212	16	-0.7078776285143817
2212	17	-0.5567461720453792
2212	18	-0.44966877169325414
2212	19	-0.37230575915423003
2212	20	-0.3968400746601404
2212	21	-0.5373633412611838
2212	22	-0.4824806825351473
2212	23	-0.540359189861384
2212	24	-0.5715547300132471
2212	25	-0.4078150823773399
2212	26	-0.5511960897616035
2212	27	-0.5833179153423558
2212	28	-0.6187411090844464
2212	29	-0.5983774202956695
2212	30	-0.4506160520889466
2212	31	-0.414533044818171
2212	32	-0.425913679491984
2212	33	-0.35693314466166015
2212	34	-0.28955961859444557
2212	35	-0.4060392829988473
2212	36	-0.5731514632195527
2212	37	-0.5147806388614313
2212	38	-0.5405483096659935
2212	39	-0.46641066458893476
2212	40	-0.42260858637917664
2212	41	-0.5390352605169468
2212	42	-0.3159182501795357
2212	43	-0.41735783748406874
2212	44	-0.33910968040871836
2212	45	-0.2883010248404503
2212	46	-0.3511134835299714
2212	47	-0.3470285555590493
2212	48	-0.4243284657907225
2212	49	-0.3701914374539612
2212	50	-0.30855533076481834
2213	1	-0.4093675798087695
2213	2	-0.4778980329537852
2213	3	-0.5276178271935521
2213	4	-0.6526068900708495
2213	5	-0.42067124918094123
2213	6	-0.4770142194687921
2213	7	-0.6423835255820194
2213	8	-0.5171498550095635
2213	9	-0.6165249649915552
2213	10	-0.6408161331405609
2213	11	-0.6470241061769428
2213	12	-0.723019663189568
2213	13	-0.5760024129052823
2213	14	-0.7468106005123616
2213	15	-0.9444562851933398
2213	16	-0.7669900744793878
2213	17	-0.8388194274991037
2213	18	-0.7102165232494855
2213	19	-0.7516114437916244
2213	20	-0.7888363255890383
2213	21	-0.8409622694387835
2213	22	-0.6610137895285817
2213	23	-0.8725205586156193
2213	24	-0.8106335626463732
2213	25	-0.817131372125047
2213	26	-0.8122824818771903
2213	27	-0.8890551140118887
2213	28	-0.934556707910204
2213	29	-1.014784108408918
2213	30	-1.0296222387315765
2213	31	-0.8531044697718855
2213	32	-0.939100764161509
2213	33	-0.9065375141134311
2213	34	-0.9156049985621308
2213	35	-1.0897174020028686
2213	36	-1.1948354106729084
2213	37	-0.9374430758645715
2213	38	-0.9337392041841142
2213	39	-1.0050814157284407
2213	40	-0.9087943679904384
2213	41	-0.9788589832680188
2213	42	-0.8892829908467448
2213	43	-0.9474297265696379
2213	44	-0.910350838478891
2213	45	-0.8592547841396154
2213	46	-0.8648612687389274
2213	47	-0.9704196753478342
2213	48	-0.9916199588827652
2213	49	-0.9948696051362624
2213	50	-0.9615863410753249
2214	1	-0.5734120459921392
2214	2	-0.7903686526959248
2214	3	-0.7479306616928163
2214	4	-0.7251229202847327
2214	5	-0.6834120851712413
2214	6	-0.5882998853549566
2214	7	-0.7397177761074047
2214	8	-0.7975211284876735
2214	9	-0.9425584276541628
2214	10	-0.8011766775873994
2214	11	-1.0371050137627904
2214	12	-0.8811249674901163
2214	13	-0.8667621112786392
2214	14	-0.9173711854366502
2214	15	-0.8496499269478122
2214	16	-0.8408843982539338
2214	17	-0.6921849442144321
2214	18	-0.7969124409560919
2214	19	-0.9239197973752837
2214	20	-0.8055198857009813
2214	21	-0.8497637497889841
2214	22	-0.7820678848365716
2214	23	-0.9144144191543546
2214	24	-1.040732866038823
2214	25	-1.0947389039384063
2214	26	-1.0332224942319996
2214	27	-1.0995496599948638
2214	28	-0.967907570338916
2214	29	-0.936457252674856
2214	30	-0.9272114359834518
2214	31	-0.9488839294396101
2214	32	-1.0498711080500698
2214	33	-0.9938588001607087
2214	34	-1.0383636290433245
2214	35	-1.135387831439722
2214	36	-1.059488792530935
2214	37	-0.9958882905746478
2214	38	-0.9764394680670634
2214	39	-0.9861702592897572
2214	40	-0.9749709716312367
2214	41	-0.9859229674355987
2214	42	-0.9386995614931308
2214	43	-1.0284562949805718
2214	44	-0.8899476674293467
2214	45	-0.7613357819228241
2214	46	-0.8172935490366768
2214	47	-0.81305298718042
2214	48	-0.8187916342402062
2214	49	-0.8863260224137335
2214	50	-0.882311522256483
2215	1	-0.4351922734135236
2215	2	-0.493557210243349
2215	3	-0.45067780765208454
2215	4	-0.4213135456067363
2215	5	-0.3995321252235229
2215	6	-0.4059697868228227
2215	7	-0.3816770054820182
2215	8	-0.3188735896940713
2215	9	-0.39715148686700985
2215	10	-0.3613606195625465
2215	11	-0.41886487909477665
2215	12	-0.3860267724356845
2215	13	-0.42210444848394957
2215	14	-0.41017675713202095
2215	15	-0.4480426048026942
2215	16	-0.5866968785395059
2215	17	-0.42663221754104086
2215	18	-0.42551873022853215
2215	19	-0.5455223486001088
2215	20	-0.521318689047753
2215	21	-0.49062049298653676
2215	22	-0.5703553093355183
2215	23	-0.6473660450438601
2215	24	-0.6626480861538866
2215	25	-0.6955483257393951
2215	26	-0.7546171519286133
2215	27	-0.7102290358881476
2215	28	-0.5627464649735003
2215	29	-0.67574263600455
2215	30	-0.6576292996077342
2215	31	-0.49155796262365925
2215	32	-0.6778624874750889
2215	33	-0.6360102417068987
2215	34	-0.6058527608823852
2215	35	-0.6465533293887447
2215	36	-0.6853434602879673
2215	37	-0.5910832568478952
2215	38	-0.560082708297557
2215	39	-0.5872697467314794
2215	40	-0.6499997132694446
2215	41	-0.6731832236177837
2215	42	-0.6346563756457186
2215	43	-0.6520343476649728
2215	44	-0.7041341319496865
2215	45	-0.7199811359235326
2215	46	-0.7105993345892188
2215	47	-0.7309910725397586
2215	48	-0.8217683863753393
2215	49	-0.7636414463075205
2215	50	-0.8138948116797629
2216	1	-0.45936211313724584
2216	2	-0.4162632766422796
2216	3	-0.4157607238434444
2216	4	-0.3487611402471771
2216	5	-0.346623504057888
2216	6	-0.40519887720155623
2216	7	-0.2611218463777334
2216	8	-0.2982581815720877
2216	9	-0.35700341665867086
2216	10	-0.4544769522551917
2216	11	-0.6186705890348208
2216	12	-0.6101765580915597
2216	13	-0.5413349973403427
2216	14	-0.72109580641294
2216	15	-0.7308231924650102
2216	16	-0.713008944143219
2216	17	-0.6119226889939426
2216	18	-0.5928288633493395
2216	19	-0.6896378253052688
2216	20	-0.7049642928661868
2216	21	-0.8325530279769779
2216	22	-0.7507708192036802
2216	23	-0.8245180375153396
2216	24	-0.9201898046920078
2216	25	-0.9664762647889162
2216	26	-0.8071851143832305
2216	27	-0.9932270061733988
2216	28	-1.0540078397970518
2216	29	-1.1099709363745518
2216	30	-0.9358240167932834
2216	31	-1.1498562664488645
2216	32	-1.0512950146522613
2216	33	-1.1487179228489524
2216	34	-1.1634228676464247
2216	35	-1.1631451622610953
2216	36	-1.2466854680908241
2216	37	-1.2456325785689657
2216	38	-1.336293461792259
2216	39	-1.294351089850501
2216	40	-1.4923167481149022
2216	41	-1.339449069369877
2216	42	-1.3271416563071696
2216	43	-1.3838240386587017
2216	44	-1.4792588404711857
2216	45	-1.630238835036117
2216	46	-1.6324186812954053
2216	47	-1.6897125953293397
2216	48	-1.7705440464667035
2216	49	-1.731436349622605
2216	50	-1.8052868417938512
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	154767.0
3	7573.0
4	14306.0
5	11248.0
6	9524.0
7	8176.0
8	7281.0
9	6760.0
10	6754.0
11	6676.0
12	6911.0
13	7215.0
14	7666.0
15	8170.0
16	8681.0
17	9527.0
18	10683.0
19	11921.0
20	14847.0
21	19309.0
22	23898.0
23	27172.0
24	29017.0
25	31291.0
26	34736.0
27	39742.0
28	45781.0
29	52602.0
30	61300.0
31	74528.0
32	90867.0
33	113911.0
34	145649.0
35	189813.0
36	254397.0
37	352623.0
38	566214.0
39	1072579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86936518304462	16.95046017783584	12.895202126100392	25.284972513019145
2	16.081135313732684	18.943764419406794	38.79503536064297	26.18006490621755
3	19.9525041251026	23.141444274538358	27.552804420144174	29.35324718021487
4	13.27803537171483	15.583585843179524	35.46588349071419	35.67249529439145
5	15.17701580663191	36.44303192930043	33.29771919850404	15.082233065563615
6	34.258796041160366	34.700489660219176	16.84110435544634	14.199609943174119
7	30.21240796783956	30.577035999819323	20.390007452911153	18.820548579429968
8	27.284695367833873	34.46745942185657	19.071183935146117	19.176661275163447
9	26.528941883028605	16.184873669621446	18.23451139671079	39.05167305063916
10	15.23150186872622	29.017704910628595	32.398292740760816	23.352500479884377
11	36.11464214767571	22.518565818568614	22.098595950707953	19.268196083047723
12	23.967475524911837	24.27317238982505	29.010146888642268	22.74920519662085
13	28.764547378049038	21.043303724552235	24.858390228961085	25.33375866843764
14	22.461905110080448	20.53184494814488	25.839595084850885	31.16665485692379
15	24.27500401579669	28.16528765102289	22.5432005151512	25.016507818029222
16	24.273670380126923	27.280819409857095	24.13451795924463	24.310992250771353
17	23.62534830344111	27.326271748673836	25.43127517877459	23.617104769110462
18	23.659886119786808	26.816562741820416	26.16077329458466	23.362777843808114
19	24.41337220563016	26.32683334773257	26.214039313873467	23.0457551327638
20	24.629786335320247	25.92197298958599	25.821201348177176	23.627039326916588
21	24.78016423440112	25.79516446030814	25.52896127953734	23.8957100257534
22	24.38951519007467	25.6275272431838	25.838475008620055	24.144482558121474
23	24.27209655953577	25.78801035114971	25.678666027483736	24.261227061830784
24	23.891113233957395	25.916065745233407	26.032602664451325	24.16021835635787
25	24.29367187268345	25.638397162459636	25.81311712902365	24.254813835833268
26	24.00503897837848	25.831005022599722	25.987703582463993	24.176252416557805
27	24.114624498116143	25.96863337086079	25.725622612707323	24.191119518315745
28	23.761454001543207	25.83523409601083	26.00887286185381	24.394439040592157
29	24.04737282348828	26.120399807743468	25.739614391226233	24.092612977542018
30	24.266837678809487	25.978483984131113	25.91415676131918	23.840521575740222
31	24.156348731117664	25.671170450121437	26.14450106757708	24.02797975118382
32	24.093245160243065	26.0022160899422	25.603461052572147	24.30107769724259
33	23.903940664185306	26.11251829400499	25.582387390235972	24.401153651573736
34	24.244639025405153	25.91001980909084	25.69685400471387	24.14848716079014
35	24.205279643826604	26.026856987178494	25.81746113528858	23.95040223370632
36	24.331135781807752	26.056682374755937	25.583294091341806	24.028887752094505
37	24.252673469929356	25.626407033714603	25.902717823076397	24.218201673279644
38	24.272925880685403	25.823097050770627	25.724462882754352	24.17951418578962
39	24.41040622184857	25.886144507396054	25.55420643566886	24.149242835086515
40	24.259795241730394	25.68650822330531	26.066251699543326	23.987444835420966
41	24.128680953886455	25.963399349622872	25.79019127410686	24.117728422383813
42	24.238914714809482	25.91120932770341	25.783285634807214	24.066590322679893
43	24.086833230822037	25.484806922631588	26.331788277807398	24.096571568738977
44	24.207539080042213	25.55760384532768	25.84889944176812	24.38595763286198
45	24.07517689261694	25.909130678979764	25.85567481675703	24.160017611646264
46	24.111118041469446	25.83846124971671	25.71055380368013	24.33986690513371
47	24.170544014067115	25.88292739375927	25.58352137939015	24.363007212783465
48	24.15091165868709	25.976825546989566	25.867997737645954	24.004265056677387
49	24.40179733853686	25.46531577463445	25.620653870653378	24.512233016175315
50	24.236109072764098	25.92933571462439	25.596498734468735	24.238056478142774
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1781.0
1	1336.0
2	891.0
3	882.0
4	873.0
5	857.0
6	841.0
7	816.5
8	792.0
9	894.5
10	997.0
11	1228.0
12	1459.0
13	2230.0
14	3001.0
15	4117.0
16	5233.0
17	5882.0
18	6531.0
19	7199.5
20	7868.0
21	9045.5
22	10223.0
23	13297.0
24	16371.0
25	21038.0
26	25705.0
27	32319.5
28	38934.0
29	47206.5
30	55479.0
31	64986.5
32	74494.0
33	85211.5
34	95929.0
35	111391.5
36	126854.0
37	136408.5
38	145963.0
39	159457.0
40	172951.0
41	187465.5
42	201980.0
43	210046.5
44	218113.0
45	229215.0
46	240317.0
47	255378.0
48	270439.0
49	273270.0
50	276101.0
51	272986.5
52	269872.0
53	263059.0
54	256246.0
55	251712.5
56	247179.0
57	230870.5
58	214562.0
59	193198.0
60	171834.0
61	151744.5
62	131655.0
63	111878.0
64	92101.0
65	77088.5
66	62076.0
67	50871.5
68	39667.0
69	31951.0
70	24235.0
71	19378.5
72	14522.0
73	11729.5
74	8937.0
75	6952.5
76	4968.0
77	3785.5
78	2603.0
79	2083.0
80	1563.0
81	1219.0
82	875.0
83	676.5
84	478.0
85	366.0
86	254.0
87	199.0
88	144.0
89	108.5
90	73.0
91	62.5
92	52.0
93	39.5
94	27.0
95	22.0
96	17.0
97	17.0
98	17.0
99	27.5
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0841959135073213
2	0.09350712378125428
3	0.10149219198586953
4	0.10324157088582057
5	0.0955386605682942
6	0.058322035261271145
7	0.05290460382916469
8	0.05987390364025998
9	0.061284693075704375
10	0.04500418299067609
11	0.0484747250018693
12	0.05767307212096673
13	0.05107057756308697
14	0.05197348280177139
15	0.052312072266278035
16	0.0551900827145846
17	0.054879709038786834
18	0.05564153533392681
19	0.06379589827079539
20	0.06895938760452186
21	0.07999176098969701
22	0.08334943984605467
23	0.05905564576770224
24	0.07753698737202376
25	0.08521168190084125
26	0.08241831881866135
27	0.07640835582366826
28	0.09680837106019416
29	0.08501417137987903
30	0.08030213466549477
31	0.05592369322101568
32	0.05191705122435361
33	0.05561331954521792
34	0.050844851253415875
35	0.056995893191953424
36	0.05617763531939568
37	0.05894278261286668
38	0.04886974604379373
39	0.053271409082380226
40	0.05606477216456012
41	0.04387555144232058
42	0.03888135684084743
43	0.03995355681178517
44	0.03730127267314971
45	0.028356867652432272
46	0.026184251921847906
47	0.03103736757977662
48	0.025478857204125713
49	0.02471703090898574
50	0.026494625597645673
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3544115.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.063697305959124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.76742685101642	43.03865781932744
2	12.656521879206256	14.191428231641371
3	4.502863908012423	7.5734159744621
4	2.0324065968057914	4.557769129838176
5	1.1079250232847937	3.105718657156819
6	0.683434809171981	2.2989529361864323
7	0.4324111503670398	1.6969797492129508
8	0.314340514739996	1.4098473175506014
9	0.21940781760658112	1.1070732125570808
>10	1.1573279050259648	11.623677442370578
>50	0.07941675069128494	3.092800384599136
>100	0.04276380527792857	4.434502371636092
>500	0.0028400996322413864	1.0991176852604958
>1k	9.128891613369116E-4	0.7700590882007257
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8215788708887832E-5
2	0.0	0.0	0.0	0.0	2.8215788708887832E-5
3	0.0	0.0	0.0	0.0	2.8215788708887832E-5
4	0.0	0.0	0.0	0.0	2.8215788708887832E-5
5	0.0	0.0	0.0	0.0	2.8215788708887832E-5
6	5.6431577417775664E-5	0.0	0.0	0.0	1.69294732253327E-4
7	5.6431577417775664E-5	0.0	0.0	0.0	1.69294732253327E-4
8	5.6431577417775664E-5	0.0	0.0	0.0	1.69294732253327E-4
9	5.6431577417775664E-5	0.0	0.0	0.0	1.69294732253327E-4
10	1.9751052096221483E-4	0.0	0.0	2.8215788708887832E-5	2.2572630967110266E-4
11	1.9751052096221483E-4	0.0	0.0	2.8215788708887832E-5	2.2572630967110266E-4
12	1.9751052096221483E-4	0.0	0.0	2.8215788708887832E-5	4.514526193422053E-4
13	1.9751052096221483E-4	0.0	0.0	2.8215788708887832E-5	5.643157741777566E-4
14	1.9751052096221483E-4	0.0	0.0	5.6431577417775664E-5	5.643157741777566E-4
15	2.821578870888783E-4	0.0	0.0	5.6431577417775664E-5	6.77178929013308E-4
16	2.821578870888783E-4	0.0	0.0	5.6431577417775664E-5	7.053947177221958E-4
17	3.103736757977662E-4	0.0	0.0	5.6431577417775664E-5	7.053947177221958E-4
18	3.103736757977662E-4	0.0	0.0	5.6431577417775664E-5	7.336105064310837E-4
19	3.103736757977662E-4	0.0	0.0	5.6431577417775664E-5	7.336105064310837E-4
20	3.103736757977662E-4	0.0	0.0	5.6431577417775664E-5	7.618262951399714E-4
21	3.103736757977662E-4	0.0	0.0	5.6431577417775664E-5	8.182578725577472E-4
22	3.103736757977662E-4	0.0	0.0	1.1286315483555133E-4	8.46473661266635E-4
23	3.103736757977662E-4	0.0	0.0	1.69294732253327E-4	8.46473661266635E-4
24	3.103736757977662E-4	0.0	0.0	2.2572630967110266E-4	9.029052386844106E-4
25	3.9502104192442965E-4	0.0	0.0	2.821578870888783E-4	9.029052386844106E-4
26	3.9502104192442965E-4	0.0	0.0	4.796684080510932E-4	9.593368161021863E-4
27	3.9502104192442965E-4	0.0	0.0	6.77178929013308E-4	0.001015768393519962
28	3.9502104192442965E-4	0.0	0.0	0.0014954368015710551	0.001015768393519962
29	4.232368306333175E-4	0.0	0.0	0.0024547736176732415	0.001015768393519962
30	4.232368306333175E-4	0.0	0.0	0.004345231461168726	0.001015768393519962
31	4.232368306333175E-4	0.0	0.0	0.010242331301326284	0.0010439841822288498
32	4.232368306333175E-4	0.0	0.0	0.017380925844674905	0.0010439841822288498
33	4.514526193422053E-4	0.0	0.0	0.02576101509121459	0.0010439841822288498
34	4.514526193422053E-4	0.0	0.0	0.03696268320864306	0.0010721999709377376
35	6.489631403044201E-4	0.0	0.0	0.052001698590480275	0.0010721999709377376
36	6.489631403044201E-4	0.0	0.0	0.07087806123672623	0.0010721999709377376
37	6.489631403044201E-4	2.8215788708887832E-5	0.0	0.09367641851350761	0.0010721999709377376
38	6.489631403044201E-4	2.8215788708887832E-5	0.0	0.11949386518213997	0.001156847337064401
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2230	0.0	23.488808	1
GTATCAA	4360	0.0	21.604597	1
TATCAAC	5775	0.0	16.424637	2
ATCAACG	5730	0.0	16.170235	3
TCAACGC	5730	0.0	16.016373	4
CAACGCA	5890	0.0	15.617334	5
CGCTAGC	85	0.002976107	15.5336	24
AACGCAG	6180	0.0	14.949574	6
CGTATAC	135	1.9510184E-5	14.67228	3
ACCGTCG	135	1.9604113E-5	14.664822	8
GTACATG	9635	0.0	14.322034	1
ATCGTCG	205	2.9534931E-8	13.943557	44
TACATGG	9970	0.0	13.840215	2
ACATGGG	10050	0.0	13.226951	3
GTGTAGG	720	0.0	12.838245	1
ACGCAGA	7175	0.0	12.753616	7
TATACCG	125	0.0026613164	12.323497	5
CGCCTTA	270	6.5647328E-9	12.219305	33
GTCTTAG	815	0.0	12.151889	1
GTGGTAT	1145	0.0	12.109436	1
>>END_MODULE