FastQCFastQC Report
Mon 6 Feb 2017
SRR2077132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3544115
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27550.024.1970791
GTATCAA52950.021.4815941
CCCTATA3050.017.3124872
CGCGATC1105.621995E-516.00138723
TATCAAC71450.015.7965842
CCTATAC4150.015.37482553
ATCAACG72300.015.3678593
TCAACGC72550.015.3155534
CAACGCA74600.014.8656085
TATACTG7300.014.4680285
AACGCAG77300.014.3738196
GTACATG92650.013.98658851
TACATGG91900.013.9094082
TCGATCG950.00625691813.890058541
ATATCGA9850.013.84500834
GACCGTG4250.013.460567
AATATCG9850.013.39839533
TATCGAA10300.013.2399435
CGTTCGC1505.2686595E-513.20300715
CGGGTAT1000.00877702113.19723137
Produced by FastQC (version 0.11.5)