FastQCFastQC Report
Mon 6 Feb 2017
SRR2077131_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077131_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248977
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45350.020.6725541
GGTATCA23050.020.4317071
ATCAACG55200.016.3851783
TCAACGC55450.016.1914964
TATCAAC57000.016.1377562
CAACGCA56000.016.0317335
AACGCAG58700.015.2886736
GTACATG88750.014.8532091
TGCGATA2102.5538611E-914.66436213
CTATGCG900.00436745414.6634599
TAGTACT4450.014.3405684
GCGATAC2153.5506673E-914.32553714
TACATGG90150.014.0604262
GTATAAT7250.013.9630931
ACATGGG91500.013.7329373
GTCCTAA5100.012.9453231
GTATAGC7500.012.9108021
ACGCAGA69950.012.7038317
CGCACGA2603.7380232E-912.69070335
TACGTTG1404.3559578E-412.5739075
Produced by FastQC (version 0.11.5)