FastQCFastQC Report
Mon 6 Feb 2017
SRR2077131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3248977
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34570.10640272307252405No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29350.023.8383161
GTATCAA57500.022.1165081
GCACCGT4850.017.2387246
CGTGCCG3500.016.9745810
TATCAAC77050.016.2483622
ATCAACG77250.016.149583
TCAACGC77400.015.9761454
ACCGTGC5800.015.9335058
CAACGCA79050.015.6419535
GTGCCGA4150.015.37635111
AACGCAG81600.015.2073036
CCGACTA900.0043557314.6693929
GTACATG85350.014.4358071
TACATGG86600.013.9483992
ATACCGT2551.891749E-1013.8052116
ACATGGG88900.013.340243
GCGCACT3800.013.3119142
AGCACCG6350.013.1663825
TACCGAT3400.012.9425847
AATCGAG1200.001961954212.83413822
Produced by FastQC (version 0.11.5)