FastQCFastQC Report
Mon 6 Feb 2017
SRR2077130_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077130_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3097275
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA16750.024.0437951
GTATCAA33150.022.5715871
GTATACG1259.931682E-1021.1270071
TCAACGC43100.016.6956044
TATCAAC44450.016.486642
ATCAACG44500.016.3197563
CAACGCA43900.016.2401895
AACGCAG45050.015.9667366
TAATCGT850.002983425715.52778713
ATACCGT1901.0801159E-815.05057056
CTATACT3950.015.0417484
ATACGAC2203.0377123E-1015.0047333
GTGTACG3000.014.6715321
GCGATAC2551.2732926E-1114.66584414
TTACGCG900.004370646614.66181643
GTATTAG4850.014.5202781
GTACATG82100.014.4213471
TATCTCG1554.562311E-614.1964225
ATAGGAC6150.013.9556233
TACATGG84850.013.5906152
Produced by FastQC (version 0.11.5)