##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077130_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3097275 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.665267049261043 31.0 27.0 31.0 2.0 34.0 2 26.808146193024513 31.0 27.0 33.0 2.0 34.0 3 26.911177083081096 31.0 27.0 34.0 2.0 34.0 4 30.13588137959981 35.0 30.0 37.0 2.0 37.0 5 30.042772759926063 35.0 30.0 37.0 2.0 37.0 6 30.07904012397995 35.0 30.0 37.0 2.0 37.0 7 29.856214898580202 35.0 30.0 37.0 2.0 37.0 8 29.734084316052012 35.0 30.0 37.0 2.0 37.0 9 30.873776464795668 37.0 30.0 39.0 2.0 39.0 10 30.786002857350415 37.0 30.0 39.0 2.0 39.0 11 30.797089699816773 37.0 30.0 39.0 2.0 39.0 12 30.678115763049778 37.0 30.0 39.0 2.0 39.0 13 30.6105492731514 37.0 30.0 39.0 2.0 39.0 14 31.307590704732387 38.0 30.0 40.0 2.0 41.0 15 31.333446658756486 38.0 30.0 40.0 2.0 41.0 16 31.270192669362586 38.0 30.0 40.0 2.0 41.0 17 31.091184993260203 37.0 29.0 40.0 2.0 41.0 18 31.05560662194994 37.0 29.0 40.0 2.0 41.0 19 31.18851248274693 38.0 29.0 40.0 2.0 41.0 20 31.155340420208088 38.0 29.0 40.0 2.0 41.0 21 31.088782235997773 38.0 29.0 40.0 2.0 41.0 22 30.99942013544164 38.0 29.0 40.0 2.0 41.0 23 30.92570178624759 37.0 29.0 40.0 2.0 41.0 24 30.93571768732192 37.0 29.0 40.0 2.0 41.0 25 30.821114366661018 37.0 29.0 40.0 2.0 41.0 26 30.754184888329256 37.0 29.0 40.0 2.0 41.0 27 30.670592698420386 37.0 28.0 40.0 2.0 41.0 28 30.542132681147137 37.0 28.0 40.0 2.0 41.0 29 30.512606404016434 37.0 28.0 40.0 2.0 41.0 30 30.329126732369584 37.0 27.0 40.0 2.0 41.0 31 30.314720843321954 37.0 27.0 40.0 2.0 41.0 32 30.18075792430443 37.0 27.0 40.0 2.0 41.0 33 30.124552388793376 37.0 27.0 40.0 2.0 41.0 34 30.033260527399083 37.0 27.0 40.0 2.0 41.0 35 29.962717227240073 37.0 27.0 40.0 2.0 41.0 36 29.8602423097723 37.0 27.0 40.0 2.0 41.0 37 29.760434898418772 37.0 26.0 40.0 2.0 41.0 38 29.66429716444294 36.0 26.0 40.0 2.0 41.0 39 29.54507397631789 36.0 26.0 40.0 2.0 41.0 40 29.471722724007392 36.0 26.0 40.0 2.0 41.0 41 29.346591439249018 36.0 25.0 40.0 2.0 41.0 42 29.194056711141243 36.0 25.0 40.0 2.0 41.0 43 29.095481996270916 36.0 25.0 40.0 2.0 41.0 44 28.939998869974414 36.0 24.0 40.0 2.0 41.0 45 28.787138694497582 36.0 24.0 40.0 2.0 41.0 46 28.621994818025524 36.0 24.0 40.0 2.0 41.0 47 28.440921132285638 35.0 23.0 40.0 2.0 41.0 48 28.24927589574707 35.0 23.0 39.0 2.0 41.0 49 28.03378033916911 35.0 22.0 39.0 2.0 41.0 50 27.804644728027057 35.0 19.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 3.151748071810381 1101 2 3.4278886769671786 1101 3 3.574117959502182 1101 4 3.95699999449263 1101 5 4.134991447078026 1101 6 4.160786477829621 1101 7 4.193613048322618 1101 8 4.221834335283322 1101 9 4.6402721393174815 1101 10 4.722546944905922 1101 11 4.698810628763052 1101 12 4.887306862807176 1101 13 4.89533646362856 1101 14 5.416468624621043 1101 15 5.364578848016798 1101 16 5.303289877918559 1101 17 5.416339552779576 1101 18 5.463949091526452 1101 19 5.421984039605803 1101 20 5.461695264042632 1101 21 5.411061311897232 1101 22 5.426427564903015 1101 23 5.578733971228278 1101 24 5.46896026609701 1101 25 5.501082537324876 1101 26 5.544791438746184 1101 27 5.419190834108356 1101 28 5.629592810890582 1101 29 5.5325969736217395 1101 30 5.763410981481936 1101 31 5.678137835891583 1101 32 5.7263294056128125 1101 33 5.740258108943195 1101 34 5.733034282967754 1101 35 5.755519880074733 1101 36 5.7632527355414815 1101 37 5.859270567686959 1101 38 5.949511583882689 1101 39 5.927964082957864 1101 40 5.836809774478748 1101 41 5.861736017339478 1101 42 5.96198598759571 1101 43 6.022844186363997 1101 44 6.006905157205551 1101 45 6.063404151322665 1101 46 5.9973291919971 1101 47 6.052828283753872 1101 48 6.155758209731907 1101 49 6.067091905151255 1101 50 6.15288586470556 1102 1 1.729037891744877 1102 2 1.7950903041643826 1102 3 1.9393088782759094 1102 4 2.1582475874176232 1102 5 2.2191038222287354 1102 6 2.2486584708305735 1102 7 2.4679220960242887 1102 8 2.403206274627845 1102 9 2.568841191553929 1102 10 2.752351510973874 1102 11 2.59757426188823 1102 12 2.712648033220159 1102 13 2.7703590479506133 1102 14 3.051150773000426 1102 15 2.793687023635215 1102 16 2.969990751622589 1102 17 3.2483296840919422 1102 18 3.2335057495551993 1102 19 3.1596525732430862 1102 20 3.0941853469874445 1102 21 3.0109152367581054 1102 22 3.0594982291354427 1102 23 2.923854571934566 1102 24 2.9876170913734335 1102 25 3.20595965899291 1102 26 3.1700985556695933 1102 27 3.035872318137592 1102 28 3.1811847859366758 1102 29 3.0161688946428917 1102 30 3.1180146942335902 1102 31 3.285053679925916 1102 32 3.286150604319534 1102 33 3.316391287432104 1102 34 3.3022549101695624 1102 35 3.35879996814975 1102 36 3.3538072085850565 1102 37 3.3824503992859682 1102 38 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26 0.9104287278643106 1103 27 0.8495376354684865 1103 28 0.9313775960359507 1103 29 0.9783870771884473 1103 30 1.1004465817417888 1103 31 0.8748400364038069 1103 32 0.9442186450460142 1103 33 0.9537285987859896 1103 34 0.9757068292785256 1103 35 0.9656181952189087 1103 36 1.0561844075498819 1103 37 1.086881144185007 1103 38 0.8567745805219502 1103 39 1.0985505123627668 1103 40 1.0271280660744395 1103 41 1.0171340258783346 1103 42 1.0874043206673463 1103 43 1.059545999826046 1103 44 0.9867864647490272 1103 45 0.9801938603620926 1103 46 0.9158584277005417 1103 47 0.980380406878055 1103 48 1.121006161554714 1103 49 1.1182058399693666 1103 50 1.0969623915635438 1104 1 -0.004402916597108231 1104 2 0.0606425918726039 1104 3 0.08698941475774546 1104 4 0.03592487441895997 1104 5 -0.023298157213844206 1104 6 0.011496289563499573 1104 7 -0.01376449310435035 1104 8 0.06583037585503249 1104 9 0.07364150510487377 1104 10 -0.22916942860014444 1104 11 0.0353276572038439 1104 12 0.0053413131465873676 1104 13 -8.238021004345342E-4 1104 14 0.12145104326846123 1104 15 0.07611268470004262 1104 16 0.15651119311693407 1104 17 0.1942813403482404 1104 18 0.10233083397861975 1104 19 0.18150495982773762 1104 20 0.10821675167218103 1104 21 0.07260929925688231 1104 22 0.03361666073692149 1104 23 0.15656131088730874 1104 24 0.09450603352615872 1104 25 0.09578687102129635 1104 26 0.14878096310398448 1104 27 0.13539094261988538 1104 28 0.1311750635954354 1104 29 0.15152767948508838 1104 30 0.02312890673047363 1104 31 0.1388355401686745 1104 32 0.24184365499881366 1104 33 0.1299046457549764 1104 34 0.11939454952507944 1104 35 0.03579273272684702 1104 36 0.14474420302875046 1104 37 0.12707329207181672 1104 38 0.23701619148098274 1104 39 0.3358090798999349 1104 40 0.3355972340802964 1104 41 0.3918559736587568 1104 42 0.24942791805169406 1104 43 0.3244274054565075 1104 44 0.3584691175726107 1104 45 0.3944550150661499 1104 46 0.38828145658851554 1104 47 0.43362505657131933 1104 48 0.31387565896548963 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0.5765547496737504 2216 9 0.8502804957193852 2216 10 0.7850570955298402 2216 11 0.5666583517115846 2216 12 0.613614285850705 2216 13 0.5753931075124612 2216 14 0.4949630024229599 2216 15 0.6031498125596997 2216 16 0.5611295620266041 2216 17 0.5582618337052914 2216 18 0.5754632788096607 2216 19 0.5926862314147101 2216 20 0.43245488910240226 2216 21 0.49281250887695194 2216 22 0.5937053104709946 2216 23 0.5073593204324247 2216 24 0.3553118392509127 2216 25 0.21407030032244023 2216 26 0.3399053480270524 2216 27 0.33907689307134703 2216 28 0.2855789797298236 2216 29 0.1562628754190456 2216 30 0.3168740341181966 2216 31 -0.018878629214317044 2216 32 0.2980454349337158 2216 33 0.22726100056674525 2216 34 0.2836068462055721 2216 35 0.28607450562899217 2216 36 0.057398536154650515 2216 37 0.03313476390724901 2216 38 -0.15656572508370203 2216 39 -0.02016679589790371 2216 40 -0.13298219557248458 2216 41 -0.04930935855797003 2216 42 -0.10438457573102511 2216 43 -0.15665899333311728 2216 44 -0.29673142006303976 2216 45 -0.280527113869244 2216 46 -0.4459563800988704 2216 47 -0.3611150669183161 2216 48 -0.6909861959700478 2216 49 -0.6928082989046374 2216 50 -0.7020511719383045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 312371.0 3 14437.0 4 25591.0 5 19354.0 6 16104.0 7 13069.0 8 11521.0 9 10484.0 10 10010.0 11 9957.0 12 10266.0 13 10746.0 14 11170.0 15 11932.0 16 12814.0 17 13384.0 18 14759.0 19 16306.0 20 19438.0 21 24559.0 22 28899.0 23 32888.0 24 35853.0 25 39965.0 26 44281.0 27 49498.0 28 55586.0 29 63622.0 30 72848.0 31 84554.0 32 101694.0 33 121451.0 34 149533.0 35 184880.0 36 234403.0 37 306308.0 38 438571.0 39 464169.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49100456436563 16.73983115886416 12.662729733004808 25.1064345437654 2 16.490820425482408 18.719545497144814 38.663378975364935 26.12625510200784 3 20.180663802116577 23.06915157499083 27.358510994151857 29.391673628740733 4 13.716322215621851 15.159293391103459 35.202491708418144 35.921892684856545 5 15.503102876272425 36.27487447433046 32.97418842568819 15.247834223708928 6 34.731326812917295 34.1361740948497 16.647736009727645 14.484763082505358 7 30.599508794757547 30.339951112060113 20.032846079871405 19.02769401331093 8 27.41390099167269 34.16565524473288 18.972423313111356 19.448020450483074 9 27.106838672660942 15.830995452601218 18.06621834492259 38.99594752981525 10 15.692273602479156 28.33179312075708 32.095489088285674 23.88044418847809 11 36.593074915902676 22.25689623873238 21.898412632783966 19.25161621258097 12 24.47018423458493 23.70749892586359 28.611795794554045 23.21052104499743 13 29.06110846409161 20.813185925752446 24.736661040628608 25.38904456952733 14 23.153553215455428 20.2677336127757 25.289297471419125 31.289415700349743 15 24.784176229133536 27.97218203202704 22.216741496341534 25.02690024249789 16 25.11758609951402 26.915167443897865 23.787183373583957 24.180063083004157 17 24.034915262231117 27.010678674212407 25.022604598965824 23.931801464590652 18 24.011027403433495 26.611202299034005 25.530978068272603 23.846792229259897 19 24.932309558139153 26.244442857830524 25.487240339594248 23.336007244436075 20 24.978466338023892 25.677300709431584 25.498569699601376 23.84566325294315 21 25.168312851876934 25.543454378137486 25.450169831512664 23.838062938472913 22 25.11094610026154 25.111560044023 25.312642782315574 24.464851073399885 23 24.80278623898764 25.27757237201498 25.3707075983549 24.548933790642476 24 24.458472527248833 25.370358941782666 25.350552182087778 24.820616348880726 25 24.766404667641247 25.218569487906294 25.366145899980225 24.648879944472235 26 24.486931300995458 25.691676998643835 25.601169201757422 24.220222498603285 27 24.694242070585073 25.57241968482668 25.199422798303807 24.53391544628444 28 24.59849808633176 25.681030301012918 25.178690367735197 24.541781244920127 29 24.647250157204965 25.694367699986365 25.243627301768257 24.414754841040416 30 24.605857529678623 25.812745167731887 25.247090299274273 24.334307003315217 31 24.79960873106043 25.35908584173055 25.099036719905126 24.74226870730389 32 24.637736870565995 25.6762492739878 25.123252482977882 24.562761372468326 33 24.824038754761645 25.460529376192024 25.04122022194311 24.674211647103213 34 25.04240492365499 25.344270481876922 25.074416562841495 24.538908031626594 35 24.74972105082593 25.55100134192805 25.15297785915635 24.546299748089677 36 24.728094432025223 25.612264016849938 25.07139257517218 24.588248975952652 37 24.755012020838272 25.275193525481527 25.3211964109996 24.648598042680597 38 24.886886743969473 25.49090038700933 25.16917324199211 24.453039627029085 39 25.09995461380548 25.441530144670516 25.038707478708062 24.41980776281595 40 24.98191771405017 25.25408074956123 25.36259061736914 24.401410919019465 41 24.575689784706938 25.525571017252773 25.313908486075103 24.584830711965182 42 24.944817210441354 25.36712623793876 25.287961463453396 24.4000950881665 43 24.715928910846575 25.259556028702324 25.237528003369448 24.786987057081657 44 24.76497219264222 25.51807534778173 25.052137786515587 24.66481467306047 45 24.85575025691407 25.41882968615467 25.015421303553907 24.709998753377345 46 24.839229204431213 25.206193207855737 25.030348888692966 24.924228699020084 47 24.654389116918775 25.404018758988467 25.13634694504363 24.805245179049127 48 24.63314119110955 25.45219734208819 25.279776366215824 24.634885100586438 49 24.88558049410625 25.280800904246732 25.087905699983853 24.74571290166317 50 24.99370250233492 25.330634774846693 25.139190845828214 24.53647187699017 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1884.0 1 1361.0 2 838.0 3 752.0 4 666.0 5 659.5 6 653.0 7 644.5 8 636.0 9 694.0 10 752.0 11 911.0 12 1070.0 13 1551.0 14 2032.0 15 2697.5 16 3363.0 17 3592.0 18 3821.0 19 4177.0 20 4533.0 21 5306.5 22 6080.0 23 8035.5 24 9991.0 25 13201.5 26 16412.0 27 21814.0 28 27216.0 29 33910.5 30 40605.0 31 49632.0 32 58659.0 33 69398.5 34 80138.0 35 91686.0 36 103234.0 37 113139.5 38 123045.0 39 134844.5 40 146644.0 41 158160.0 42 169676.0 43 178654.0 44 187632.0 45 198041.5 46 208451.0 47 216883.5 48 225316.0 49 229507.0 50 233698.0 51 237160.0 52 240622.0 53 235349.0 54 230076.0 55 223551.5 56 217027.0 57 205054.5 58 193082.0 59 177059.0 60 161036.0 61 143557.0 62 126078.0 63 109549.5 64 93021.0 65 79152.0 66 65283.0 67 55205.0 68 45127.0 69 36567.0 70 28007.0 71 22361.0 72 16715.0 73 13707.0 74 10699.0 75 8251.5 76 5804.0 77 4530.0 78 3256.0 79 2547.0 80 1838.0 81 1454.5 82 1071.0 83 856.0 84 641.0 85 467.0 86 293.0 87 259.0 88 225.0 89 170.5 90 116.0 91 99.0 92 82.0 93 61.0 94 40.0 95 33.0 96 26.0 97 24.5 98 23.0 99 32.5 100 42.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08555908015917217 2 0.09379212372165856 3 0.10105657392385242 4 0.10244489107360502 5 0.09324325415082613 6 0.05705014892122914 7 0.05243318723716816 8 0.058051028726864747 9 0.06066623079965454 10 0.044619867464141864 11 0.047041350864873155 12 0.05698557603054298 13 0.05004399028177996 14 0.05120630231413097 15 0.0509480107513863 16 0.05395065016829309 17 0.053789217941577684 18 0.055338967318045704 19 0.06244198529352415 20 0.06847955057268083 21 0.07887578597315383 22 0.08165242027265902 23 0.057340726929316896 24 0.07658344835379487 25 0.08417076300941957 26 0.08116812359251277 27 0.07564714143884545 28 0.09543873243415583 29 0.08358960699324407 30 0.07926322331727083 31 0.055080675755301026 32 0.05156145321290489 33 0.055112962200644114 34 0.04926911559354594 35 0.05582326399819196 36 0.055371253763388785 37 0.05866447118838333 38 0.04791308488913642 39 0.05230404145579582 40 0.05501610286461487 41 0.04226295695409674 42 0.038776020857043694 43 0.0393571768732192 44 0.03719398503523258 45 0.02934837881686321 46 0.025377146039663897 47 0.03054297729455731 48 0.02531257314897773 49 0.02502199514088998 50 0.02605516139186866 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3097275.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.41725134917768 #Duplication Level Percentage of deduplicated Percentage of total 1 79.87092265967253 49.85323455139511 2 11.090074133719506 13.844238893707686 3 3.8331729892569673 7.177683658059926 4 1.7094060842147902 4.267857168649926 5 0.9654333390249474 3.012984769139801 6 0.5851862147607961 2.1915429031679095 7 0.40175122372712313 1.7553344977850889 8 0.2732452148802514 1.3644172205712572 9 0.2012905193497125 1.1307600846411765 >10 0.9962450512352492 10.863247759593216 >50 0.0523410046404639 2.2157939001908606 >100 0.020145811684405282 1.9812926442967553 >500 6.80986655642811E-4 0.2734072661503913 >1k 1.0476717759399837E-4 0.06820468265080759 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.2600511740158687E-4 2 0.0 0.0 0.0 0.0 2.2600511740158687E-4 3 0.0 0.0 0.0 0.0 2.2600511740158687E-4 4 0.0 0.0 0.0 3.228644534308384E-5 2.582915627446707E-4 5 0.0 0.0 0.0 3.228644534308384E-5 2.582915627446707E-4 6 0.0 0.0 0.0 3.228644534308384E-5 3.874373441170061E-4 7 0.0 0.0 0.0 3.228644534308384E-5 4.197237894600899E-4 8 0.0 0.0 0.0 3.228644534308384E-5 4.197237894600899E-4 9 0.0 0.0 0.0 3.228644534308384E-5 4.842966801462576E-4 10 0.0 0.0 0.0 3.228644534308384E-5 4.842966801462576E-4 11 0.0 0.0 0.0 3.228644534308384E-5 5.811560161755092E-4 12 0.0 0.0 0.0 3.228644534308384E-5 6.134424615185929E-4 13 0.0 0.0 0.0 3.228644534308384E-5 6.780153522047606E-4 14 0.0 0.0 0.0 6.457289068616767E-5 8.394475789201798E-4 15 0.0 0.0 0.0 6.457289068616767E-5 8.717340242632637E-4 16 0.0 0.0 0.0 6.457289068616767E-5 9.363069149494314E-4 17 0.0 0.0 0.0 6.457289068616767E-5 9.363069149494314E-4 18 0.0 0.0 0.0 6.457289068616767E-5 9.363069149494314E-4 19 0.0 0.0 0.0 6.457289068616767E-5 9.363069149494314E-4 20 0.0 0.0 0.0 6.457289068616767E-5 0.0010008798056355991 21 0.0 0.0 0.0 9.685933602925152E-5 0.0010008798056355991 22 0.0 0.0 0.0 1.614322267154192E-4 0.0010008798056355991 23 0.0 0.0 0.0 2.905780080877546E-4 0.0010654526963217667 24 0.0 0.0 0.0 4.197237894600899E-4 0.0010654526963217667 25 0.0 0.0 0.0 4.842966801462576E-4 0.0010654526963217667 26 0.0 0.0 0.0 7.748746882340121E-4 0.0010654526963217667 27 0.0 0.0 0.0 0.0010331662509786828 0.0010977391416648506 28 0.0 0.0 0.0 0.002227764728672785 0.0010977391416648506 29 0.0 0.0 0.0 0.0036806547691115576 0.0010977391416648506 30 0.0 0.0 0.0 0.006360429732587517 0.0011300255870079344 31 0.0 0.0 0.0 0.013754025716153716 0.0011623120323510183 32 0.0 0.0 0.0 0.02195478283329701 0.0012914578137233537 33 0.0 0.0 0.0 0.032189586007054585 0.0012914578137233537 34 0.0 0.0 0.0 0.04529788281634663 0.0013560307044095212 35 0.0 0.0 0.0 0.06221598017612256 0.0013560307044095212 36 0.0 3.228644534308384E-5 0.0 0.08278244585966696 0.0013560307044095212 37 0.0 3.228644534308384E-5 0.0 0.10951562260374038 0.0013560307044095212 38 0.0 3.228644534308384E-5 0.0 0.139025433647319 0.0013560307044095212 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1675 0.0 24.043795 1 GTATCAA 3315 0.0 22.571587 1 GTATACG 125 9.931682E-10 21.127007 1 TCAACGC 4310 0.0 16.695604 4 TATCAAC 4445 0.0 16.48664 2 ATCAACG 4450 0.0 16.319756 3 CAACGCA 4390 0.0 16.240189 5 AACGCAG 4505 0.0 15.966736 6 TAATCGT 85 0.0029834257 15.527787 13 ATACCGT 190 1.0801159E-8 15.0505705 6 CTATACT 395 0.0 15.041748 4 ATACGAC 220 3.0377123E-10 15.004733 3 GTGTACG 300 0.0 14.671532 1 GCGATAC 255 1.2732926E-11 14.665844 14 TTACGCG 90 0.0043706466 14.661816 43 GTATTAG 485 0.0 14.520278 1 GTACATG 8210 0.0 14.421347 1 TATCTCG 155 4.562311E-6 14.196422 5 ATAGGAC 615 0.0 13.955623 3 TACATGG 8485 0.0 13.590615 2 >>END_MODULE