FastQCFastQC Report
Mon 6 Feb 2017
SRR2077130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3097275
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20900.023.8939721
GTATCAA40000.021.7243081
GTATACG1051.7950806E-618.856541
AACGTCG902.2148578E-417.11305222
TATCAAC55000.015.6402592
GTATAGG3950.015.59443951
GTATTAG4500.015.1550721
ATCAACG57550.014.8328043
TCAACGC57550.014.7950534
CAACGCA57450.014.7066285
TATGCGA1201.1798493E-414.66975212
CGTGCCG3350.014.45033410
GTACATG85700.014.0672281
AACGCAG59800.014.0551066
TATACGA950.00624205613.8949672
CGAATCG2855.456968E-1213.8920537
CTATACT5250.013.8294714
TAGATCG1453.8355018E-513.656725
TACATGG87950.013.3828252
TAGGCCG3650.013.26177823
Produced by FastQC (version 0.11.5)