FastQCFastQC Report
Mon 6 Feb 2017
SRR2077129_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077129_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568487
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51150.018.8016071
GGTATCA30600.016.397311
TAGCGCG1301.3689047E-515.23256629
TATCAAC63200.014.9382392
TCAACGC62550.014.6360944
ATCAACG63350.014.590223
GTACATG97650.014.4909841
CAACGCA63750.014.3928925
CGCGCAA1100.001017020314.00302621
AACGCAG65900.013.95181756
TACATGG99600.013.8758462
GTATAGA5450.013.325291
ACATGGG104100.013.0223443
GTATAGC7450.012.1111891
GTGTAGC11850.012.0713041
GTATTGG7400.011.895631
GTGTACG3900.011.8498771
TCTATAC4650.011.8316763
ACGCAGA76050.011.8003477
TACACTG18150.011.7594575
Produced by FastQC (version 0.11.5)