##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077129_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3568487
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.709602136703875	31.0	30.0	34.0	16.0	34.0
2	28.790046033515043	31.0	30.0	34.0	16.0	34.0
3	28.844133662249575	31.0	30.0	34.0	16.0	34.0
4	32.22568556365765	35.0	33.0	37.0	19.0	37.0
5	32.17893437751069	35.0	33.0	37.0	19.0	37.0
6	32.214805042024814	36.0	33.0	37.0	19.0	37.0
7	32.06805321134699	36.0	33.0	37.0	18.0	37.0
8	31.996290584777245	35.0	33.0	37.0	17.0	37.0
9	33.30255371534211	38.0	33.0	39.0	17.0	39.0
10	33.23465266932456	38.0	33.0	39.0	17.0	39.0
11	33.2813520127718	38.0	33.0	39.0	17.0	39.0
12	33.16556232375233	38.0	33.0	39.0	17.0	39.0
13	33.10698707883761	38.0	33.0	39.0	17.0	39.0
14	34.000873759663406	39.0	33.0	41.0	16.0	41.0
15	34.03833249217385	39.0	33.0	41.0	17.0	41.0
16	33.9968675800136	39.0	33.0	41.0	16.0	41.0
17	33.82301210569073	39.0	33.0	41.0	11.0	41.0
18	33.800992409388066	39.0	33.0	41.0	13.0	41.0
19	33.923592267535234	39.0	33.0	41.0	14.0	41.0
20	33.90208707499845	39.0	33.0	41.0	12.0	41.0
21	33.83886448234224	39.0	33.0	41.0	11.0	41.0
22	33.755510668807254	39.0	33.0	41.0	10.0	41.0
23	33.7149733206258	39.0	33.0	41.0	10.0	41.0
24	33.71564839664541	39.0	33.0	41.0	10.0	41.0
25	33.619920431264006	39.0	33.0	41.0	10.0	41.0
26	33.56248600597396	39.0	33.0	41.0	10.0	41.0
27	33.50586284887685	39.0	33.0	41.0	9.0	41.0
28	33.38705591473361	38.0	33.0	41.0	9.0	41.0
29	33.368662405103336	38.0	33.0	41.0	8.0	41.0
30	33.204297787829965	38.0	32.0	40.0	8.0	41.0
31	33.204303672676964	38.0	32.0	40.0	8.0	41.0
32	33.0708454311309	38.0	32.0	40.0	6.0	41.0
33	33.03377649967619	38.0	32.0	40.0	2.0	41.0
34	32.94926421197555	38.0	32.0	40.0	2.0	41.0
35	32.87816601265466	38.0	32.0	40.0	2.0	41.0
36	32.7896923822337	38.0	32.0	40.0	2.0	41.0
37	32.696210186558055	38.0	32.0	40.0	2.0	41.0
38	32.60452707267814	38.0	32.0	40.0	2.0	41.0
39	32.491584248450394	38.0	31.0	40.0	2.0	41.0
40	32.422407591788904	38.0	31.0	40.0	2.0	41.0
41	32.309672138360035	38.0	31.0	40.0	2.0	41.0
42	32.16102482648809	38.0	31.0	40.0	2.0	41.0
43	32.06937057638153	38.0	31.0	40.0	2.0	41.0
44	31.916555391682806	38.0	31.0	40.0	2.0	41.0
45	31.764717091585315	38.0	31.0	40.0	2.0	41.0
46	31.625242014332684	38.0	30.0	40.0	2.0	41.0
47	31.45529912256931	38.0	30.0	40.0	2.0	41.0
48	31.29118559210108	38.0	30.0	40.0	2.0	41.0
49	31.10968934453173	37.0	30.0	40.0	2.0	41.0
50	30.889188891538627	37.0	30.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.7634429376049852
1101	2	1.8901802869530364
1101	3	1.9403765196224363
1101	4	2.145739498256603
1101	5	2.2353883460818267
1101	6	2.161853135766407
1101	7	2.2613556398639574
1101	8	2.4128636646376336
1101	9	2.5950279028685515
1101	10	2.5691383466350075
1101	11	2.6021677102455385
1101	12	2.759568626935234
1101	13	2.57430863106255
1101	14	3.015604410061826
1101	15	3.0386072285910473
1101	16	3.073451232011493
1101	17	3.1522835443540984
1101	18	3.0042594801682156
1101	19	2.9102586412761084
1101	20	3.0272684964386016
1101	21	3.141332865793295
1101	22	2.959547087738173
1101	23	3.1256935172481377
1101	24	3.017685096599166
1101	25	3.0279369273690264
1101	26	3.093045083271562
1101	27	3.062438465938655
1101	28	3.146273057606699
1101	29	3.027865238812531
1101	30	3.1422950163109604
1101	31	3.1631959902054163
1101	32	3.1310711386218344
1101	33	3.1677410648754716
1101	34	3.335802895834135
1101	35	3.308546243605008
1101	36	3.3252336892432197
1101	37	3.3736752142369255
1101	38	3.092151216163323
1101	39	3.364121169023342
1101	40	3.1270487500192203
1101	41	3.2055799506171496
1101	42	3.2163990280240355
1101	43	3.1984938498812028
1101	44	3.12945494116196
1101	45	3.122034369591873
1101	46	3.0931596880522747
1101	47	3.0998954112117936
1101	48	3.1476314601613637
1101	49	3.1671003639671333
1101	50	3.2331169700359474
1102	1	0.9589717045653252
1102	2	0.9008402985637751
1102	3	0.903485679304989
1102	4	1.078412411620846
1102	5	1.1003801455134479
1102	6	0.9912835760693781
1102	7	1.2345399913564137
1102	8	1.255367821488619
1102	9	1.3868361750666551
1102	10	1.5025913968456308
1102	11	1.330956456880621
1102	12	1.4239680427780712
1102	13	1.349740575072417
1102	14	1.7031218649570832
1102	15	1.544220997658904
1102	16	1.7657725003488238
1102	17	1.8674773518744416
1102	18	1.7962968478665715
1102	19	1.761340828620149
1102	20	1.7454058781008754
1102	21	1.753460122969841
1102	22	1.644900378050579
1102	23	1.6518664053997512
1102	24	1.59115897308574
1102	25	1.7525949245555594
1102	26	1.68809167491235
1102	27	1.5558421317252495
1102	28	1.735671212818211
1102	29	1.5005785505621319
1102	30	1.6523530764644079
1102	31	1.7837362644670733
1102	32	1.9338491660117967
1102	33	1.7992981255745448
1102	34	1.7899590680143902
1102	35	1.8676655697943332
1102	36	1.8921459796875055
1102	37	1.7968554121107871
1102	38	1.74991728695003
1102	39	1.8517696487666129
1102	40	1.869276715476623
1102	41	1.8466736064738498
1102	42	1.7715577899935653
1102	43	1.6415289911107038
1102	44	1.6209060829706985
1102	45	1.6135453105788145
1102	46	1.5805484454188914
1102	47	1.5015364299570528
1102	48	1.4549115575726077
1102	49	1.454615944077343
1102	50	1.4521167528194674
1103	1	0.4463029283231421
1103	2	0.5269610615609217
1103	3	0.5475776692426813
1103	4	0.595503951692578
1103	5	0.7543032367964528
1103	6	0.7226851380529453
1103	7	0.6222397532640898
1103	8	0.7533428126137096
1103	9	0.8020423330277495
1103	10	0.791754064703774
1103	11	0.7083477851787237
1103	12	0.814769814087029
1103	13	0.8335734024340624
1103	14	0.9167413273213612
1103	15	0.8229336924478048
1103	16	0.7629257975296682
1103	17	1.0028048359302133
1103	18	0.7568798680279087
1103	19	0.8737415979827432
1103	20	0.8391013280280433
1103	21	0.7083147734209305
1103	22	0.76324228535983
1103	23	0.8351978770428943
1103	24	0.7396180291629122
1103	25	0.8704433564513607
1103	26	0.7956810972477371
1103	27	0.7715375571699141
1103	28	0.9357893408861742
1103	29	0.8470599561025196
1103	30	0.939025873957398
1103	31	0.9083883069197327
1103	32	0.9890373905408154
1103	33	0.9431449079618304
1103	34	1.0414049127218874
1103	35	0.9743404942046112
1103	36	0.8903288425487759
1103	37	1.0259065009700237
1103	38	0.9306547610903877
1103	39	1.0163568584959606
1103	40	0.9514223159711719
1103	41	0.9112169007167878
1103	42	0.9307136333545216
1103	43	0.8258539167451033
1103	44	0.8393706323090555
1103	45	0.6771197863472551
1103	46	0.7200715415983936
1103	47	0.8000062736362885
1103	48	0.6939021593524828
1103	49	0.7594330291907632
1103	50	0.7560887325969254
1104	1	0.3132374813215151
1104	2	0.3486111458313985
1104	3	0.4156394220159818
1104	4	0.25665631428963565
1104	5	0.34839450921489856
1104	6	0.23525925268708647
1104	7	0.28175574361965516
1104	8	0.3719601457752617
1104	9	0.48151879119620133
1104	10	0.18936602874487107
1104	11	0.3538926904303139
1104	12	0.27359487857169285
1104	13	0.37713558510510836
1104	14	0.5817492942336955
1104	15	0.4484032275534915
1104	16	0.4435088808562071
1104	17	0.5583463098878809
1104	18	0.45358102730568106
1104	19	0.5027456287671015
1104	20	0.5338621628973499
1104	21	0.46086918112175823
1104	22	0.4166687807334952
1104	23	0.4191889846014689
1104	24	0.5033861946433049
1104	25	0.5812240612733177
1104	26	0.5183620158353577
1104	27	0.6619225062789624
1104	28	0.5257183617447581
1104	29	0.640146556176667
1104	30	0.6142836154034867
1104	31	0.5760337030698466
1104	32	0.6847991509392131
1104	33	0.6540060707103379
1104	34	0.6133325242520868
1104	35	0.5536597833351635
1104	36	0.5660362018197134
1104	37	0.6829084440552435
1104	38	0.6748169600633673
1104	39	0.7241769877019237
1104	40	0.7001396562319542
1104	41	0.7416091594021665
1104	42	0.629582292362791
1104	43	0.5808365236345097
1104	44	0.7069287342382111
1104	45	0.6838701012111983
1104	46	0.6376909072428631
1104	47	0.5375516626871679
1104	48	0.5275069505431311
1104	49	0.7589308244858124
1104	50	0.7564934543728867
1105	1	0.2705193315260459
1105	2	0.34011039176179025
1105	3	0.26949826459365056
1105	4	0.3635618059853485
1105	5	0.3094988385838846
1105	6	0.25479871666520637
1105	7	0.2901388034665544
1105	8	0.33066979378343575
1105	9	0.3177906602793996
1105	10	0.15898967900285044
1105	11	0.25004796865457024
1105	12	0.17556310545233345
1105	13	0.2502349752618045
1105	14	0.3326557495254221
1105	15	0.34333788612565286
1105	16	0.22180051806018497
1105	17	0.35125144289644084
1105	18	0.23800851301142245
1105	19	0.19880748426677997
1105	20	0.29061732284502284
1105	21	0.4145093046884796
1105	22	0.37605600806425343
1105	23	0.32586150498239164
1105	24	0.3308216040288343
1105	25	0.3150012323888802
1105	26	0.46564912486447696
1105	27	0.46872510711150284
1105	28	0.3773856621254623
1105	29	0.3858739020895996
1105	30	0.37654673347192613
1105	31	0.17145813742997262
1105	32	0.3381662138900623
1105	33	0.38868584598983347
1105	34	0.47792552454239257
1105	35	0.37857668038068226
1105	36	0.437566273053676
1105	37	0.4549806037952706
1105	38	0.4957595558412322
1105	39	0.5108651793550791
1105	40	0.5271427686946524
1105	41	0.4927724191670677
1105	42	0.5055504596292266
1105	43	0.5343437676372105
1105	44	0.4963290278506989
1105	45	0.5353038604354339
1105	46	0.4830019994677883
1105	47	0.45186347429025275
1105	48	0.4959528445145054
1105	49	0.501594457340456
1105	50	0.5354860907660566
1106	1	0.18637777812223177
1106	2	0.17523808936962126
1106	3	0.15942573816156624
1106	4	0.1435000517391103
1106	5	0.22277897713205164
1106	6	0.1082472120980782
1106	7	0.17667212621995532
1106	8	0.2533944220836304
1106	9	0.2804979823044462
1106	10	0.2628458202998374
1106	11	0.22933214423152748
1106	12	0.27344798180302377
1106	13	0.3555128258990834
1106	14	0.42345467174355633
1106	15	0.43854655329633374
1106	16	0.3155519033091565
1106	17	0.5187561469264423
1106	18	0.2714238408387857
1106	19	0.40162575111182974
1106	20	0.294398914787223
1106	21	0.5332185600006696
1106	22	0.43334626269536614
1106	23	0.41201404192583624
1106	24	0.37041024416680557
1106	25	0.32595927119226076
1106	26	0.3519672977881214
1106	27	0.4767173355127028
1106	28	0.3885682184091266
1106	29	0.3727617934767764
1106	30	0.5049404863143678
1106	31	0.3411467591755084
1106	32	0.4793729227931962
1106	33	0.39690828593339234
1106	34	0.44903244531361963
1106	35	0.44021937198464656
1106	36	0.5005180172857138
1106	37	0.3839215866173262
1106	38	0.5333200078003699
1106	39	0.6204757826538199
1106	40	0.6714586804021607
1106	41	0.6629790781094016
1106	42	0.5819617727142301
1106	43	0.5313197002449854
1106	44	0.6015030996144901
1106	45	0.63476280820705
1106	46	0.6250590216423078
1106	47	0.6618411670367017
1106	48	0.7291504632454959
1106	49	0.8184519608040368
1106	50	0.8020462907170298
1107	1	0.06887336370623132
1107	2	0.10630646606835725
1107	3	0.1097217455120294
1107	4	-0.05495126264106176
1107	5	-0.040546647131321834
1107	6	-0.013662637224143737
1107	7	-0.01595569199984226
1107	8	-0.00673333784826724
1107	9	0.08466849319667347
1107	10	-0.0685993817022208
1107	11	-0.1266720681990705
1107	12	-0.10494330318101674
1107	13	0.05387361560729431
1107	14	-0.14298151585687435
1107	15	-0.014787978309264815
1107	16	-0.04441808675097292
1107	17	-0.02313090350139646
1107	18	0.08843481847193146
1107	19	-0.034085672887648855
1107	20	0.05650697626501966
1107	21	0.02485805340509728
1107	22	-0.23233773744357222
1107	23	-0.24374293465956498
1107	24	-0.2372281705546726
1107	25	-0.17384463167012854
1107	26	0.20365065223991508
1107	27	0.08319796228126819
1107	28	-0.08521894131446572
1107	29	0.0280568682208866
1107	30	0.023060646932016482
1107	31	0.04693598443181912
1107	32	0.05841493861229452
1107	33	0.03467571437132477
1107	34	-0.057596315980553925
1107	35	0.04112309608161979
1107	36	-0.0015808822863832006
1107	37	0.0796574582259808
1107	38	-0.0157857012255036
1107	39	0.11585955968096329
1107	40	0.16451700826753068
1107	41	0.2612964849689092
1107	42	0.0966875555206812
1107	43	0.10672467027549004
1107	44	0.15091202465720954
1107	45	0.30361909737592185
1107	46	0.21335667961082905
1107	47	0.17154116518100437
1107	48	0.07974014643055582
1107	49	0.09238419791253705
1107	50	0.11418517340704426
1108	1	-0.20121203338976912
1108	2	-0.18440852017115716
1108	3	-0.1371251284821291
1108	4	-0.2984042479327087
1108	5	-0.29905984447564293
1108	6	-0.3237739782277167
1108	7	-0.20749901826149397
1108	8	-0.233791481508689
1108	9	-0.28219702169111827
1108	10	-0.27708641001021306
1108	11	-0.25241014293402486
1108	12	-0.4025166886108096
1108	13	-0.3981247162293471
1108	14	-0.39779585356850333
1108	15	-0.4184505264770735
1108	16	-0.3588315393750108
1108	17	-0.5271696959200582
1108	18	-0.363607550178088
1108	19	-0.32905364381381474
1108	20	-0.44255673243861793
1108	21	-0.31792656707813904
1108	22	-0.48681845411856983
1108	23	-0.6421572196200955
1108	24	-0.3944343296818431
1108	25	-0.2529662199878189
1108	26	-0.443105955012733
1108	27	-0.48599044506542555
1108	28	-0.5046816014416535
1108	29	-0.5020237656858413
1108	30	-0.549602218452101
1108	31	-0.6164544383349693
1108	32	-0.31198498138846276
1108	33	-0.42377805363541654
1108	34	-0.4364515950142618
1108	35	-0.4556191395795537
1108	36	-0.6689926165439317
1108	37	-0.5893314862069516
1108	38	-0.38489712268206233
1108	39	-0.553376997296418
1108	40	-0.5925269476274764
1108	41	-0.3345218909243286
1108	42	-0.33755326581416867
1108	43	-0.2587155527171596
1108	44	-0.35948205944775324
1108	45	-0.39154030861691425
1108	46	-0.30682355370051084
1108	47	-0.38410720185309444
1108	48	-0.39584347659849684
1108	49	-0.5080744772029924
1108	50	-0.4220009581613411
1109	1	-0.2486688538615489
1109	2	-0.25550544384285345
1109	3	-0.327690684781377
1109	4	-0.3065919553760885
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2210	44	-0.34733181594688745
2210	45	-0.055356755970940696
2210	46	-0.13661608399473124
2210	47	-0.1700325068788331
2210	48	-0.19587114701987574
2210	49	-0.00439652780287858
2210	50	0.06096957877216269
2211	1	-0.1094350559392403
2211	2	-0.07384653334050384
2211	3	-0.1390442162054093
2211	4	-0.13025983962626242
2211	5	-0.25626112311379856
2211	6	-0.14786586857428574
2211	7	-0.09558624611005229
2211	8	-0.09126633780582694
2211	9	-0.03995032139967236
2211	10	-0.040737234758168483
2211	11	-0.05891697089581527
2211	12	-0.1506189577530037
2211	13	-0.07886396446068744
2211	14	-0.23952204654902687
2211	15	-0.12974247523597882
2211	16	-0.2555918106870365
2211	17	0.03203173912145019
2211	18	-0.2959301492147972
2211	19	-0.20939133052905134
2211	20	-0.18431385645376963
2211	21	-0.25775154450629145
2211	22	-0.2352212635981985
2211	23	-0.3160980502057811
2211	24	-0.2794033677627965
2211	25	-0.21556527884057886
2211	26	-0.3878956315526594
2211	27	-0.2423725349474708
2211	28	-0.1186390967923856
2211	29	-0.14055645754012858
2211	30	-0.6511374187037404
2211	31	-0.3962541126850141
2211	32	-0.176612872534065
2211	33	-0.27139661454047825
2211	34	-0.2536186455606355
2211	35	-0.15757752626620203
2211	36	-0.22379529484054217
2211	37	-0.11534773210937743
2211	38	-0.09870725857466311
2211	39	-0.11716304917128184
2211	40	-0.112058165304461
2211	41	-0.05127044597067254
2211	42	-0.08568930386097406
2211	43	-0.1069660204412557
2211	44	-0.24612471241215061
2211	45	-0.04682971949258885
2211	46	-0.1573629937415646
2211	47	-0.3482733826243667
2211	48	-0.32416138656494553
2211	49	-0.25541774723803456
2211	50	-0.12429467385851467
2212	1	-0.25886309262362417
2212	2	-0.3293002751813532
2212	3	-0.34869579143384044
2212	4	-0.28770345709915546
2212	5	-0.2599437993570568
2212	6	-0.36436455280243507
2212	7	-0.27865352313491343
2212	8	-0.16435357706084375
2212	9	-0.3318864655563232
2212	10	-0.3318370383386693
2212	11	-0.29496996397833186
2212	12	-0.3113330791673832
2212	13	-0.27784084193230996
2212	14	-1.0006533774090158
2212	15	-0.6411923399318908
2212	16	-0.6550045903577058
2212	17	-0.4526676598570347
2212	18	-0.2710213564580357
2212	19	-0.24031729732583074
2212	20	-0.2677447686776091
2212	21	-0.34638961145430613
2212	22	-0.43166990249205384
2212	23	-0.5554453353599484
2212	24	-0.3957456967168227
2212	25	-0.23442600501620348
2212	26	-0.38106811791598005
2212	27	-0.5122455814528237
2212	28	-0.5826605544428105
2212	29	-0.5794865914815688
2212	30	-0.5263331248443421
2212	31	-0.3207472974002812
2212	32	-0.30186934468157034
2212	33	-0.28900676057106267
2212	34	-0.2190327424589782
2212	35	-0.31783941115960346
2212	36	-0.44911037837851353
2212	37	-0.3750415844063397
2212	38	-0.4967665314906071
2212	39	-0.4573965513605458
2212	40	-0.33062970701404737
2212	41	-0.2776587614274817
2212	42	-0.28958767467977964
2212	43	-0.2968971484179015
2212	44	-0.22366487417143688
2212	45	-0.18507159906350523
2212	46	-0.13651111081060918
2212	47	-0.1317235699304149
2212	48	-0.19941695610286203
2212	49	-0.0725416487476025
2212	50	-0.021380380424211864
2213	1	-0.5056070204029375
2213	2	-0.5991678048304614
2213	3	-0.5435920394331042
2213	4	-0.7454513656713857
2213	5	-0.4848525529647496
2213	6	-0.643865537658872
2213	7	-0.7276637344574226
2213	8	-0.5816164080378385
2213	9	-0.6984824898547544
2213	10	-0.8211172180630371
2213	11	-0.7248628230531722
2213	12	-0.8437964294612499
2213	13	-0.7271208943731367
2213	14	-0.9434327409276406
2213	15	-0.978722529998052
2213	16	-0.7973381432551534
2213	17	-0.8622527473167381
2213	18	-0.8646753170446857
2213	19	-0.8643147007414811
2213	20	-0.9143127269540585
2213	21	-0.9065571802431052
2213	22	-0.859556136458302
2213	23	-0.9138245703221983
2213	24	-1.0183693924582329
2213	25	-1.0501557056771205
2213	26	-1.1278884158231222
2213	27	-1.1039858339197366
2213	28	-1.1555437495143934
2213	29	-1.0160226970505377
2213	30	-1.197821928665391
2213	31	-1.0480451631734198
2213	32	-1.0889561106930472
2213	33	-1.1530895712463298
2213	34	-1.2029892878562443
2213	35	-1.2285522308401866
2213	36	-1.3353634371612415
2213	37	-1.2094230977440645
2213	38	-1.16164804641938
2213	39	-1.339507167126527
2213	40	-1.1550846947789175
2213	41	-1.2239276033699582
2213	42	-1.1770979766918153
2213	43	-1.1076306516747714
2213	44	-1.0180192124720087
2213	45	-1.045544330154737
2213	46	-1.2264703482548818
2213	47	-1.2688085857931242
2213	48	-1.3394845436659857
2213	49	-1.374765610511627
2213	50	-1.2592313250272298
2214	1	-0.2811134760734184
2214	2	-0.4116429251506588
2214	3	-0.3535517476541763
2214	4	-0.3683790217228555
2214	5	-0.32930435377980416
2214	6	-0.23031351524785748
2214	7	-0.36134803382088165
2214	8	-0.4851080909476124
2214	9	-0.4564859431756503
2214	10	-0.3237669911034544
2214	11	-0.47183951692152704
2214	12	-0.37498930934433616
2214	13	-0.37937153119334965
2214	14	-0.39860171332708205
2214	15	-0.27559695646719007
2214	16	-0.2685673455500961
2214	17	-0.14636837603232777
2214	18	-0.25100874922806327
2214	19	-0.354301566831424
2214	20	-0.32303569421272726
2214	21	-0.3820880098191992
2214	22	-0.2763880226941353
2214	23	-0.4624587922075989
2214	24	-0.5170807566451856
2214	25	-0.5047155580701599
2214	26	-0.5571184707210293
2214	27	-0.578927546823131
2214	28	-0.48890465501039415
2214	29	-0.42708687952510616
2214	30	-0.31845819288262334
2214	31	-0.3551254441488396
2214	32	-0.48204543408583334
2214	33	-0.4958504216890631
2214	34	-0.4417159012417713
2214	35	-0.5400651708914737
2214	36	-0.5288158196379555
2214	37	-0.5186395526514005
2214	38	-0.37561457318791014
2214	39	-0.44288968817370034
2214	40	-0.4493271890187813
2214	41	-0.33806397921318876
2214	42	-0.21302103322852162
2214	43	-0.3807808398341521
2214	44	-0.21795682938694227
2214	45	-0.1806522807201567
2214	46	-0.12926003650506246
2214	47	-0.14416391528160588
2214	48	-0.013721319740930937
2214	49	-0.07730016093542247
2214	50	-0.06363081783601743
2215	1	-0.3286506563793061
2215	2	-0.46205606717098746
2215	3	-0.43983469719629653
2215	4	-0.3920443480707725
2215	5	-0.3801761140219959
2215	6	-0.37030027437203117
2215	7	-0.3257539948730823
2215	8	-0.299564363400588
2215	9	-0.31798030888786855
2215	10	-0.466186547005627
2215	11	-0.4988039631806487
2215	12	-0.42315879369323994
2215	13	-0.45375917910626384
2215	14	-0.44689630383194867
2215	15	-0.4183362502361092
2215	16	-0.5595880845705992
2215	17	-0.3684584690278072
2215	18	-0.35419830449190215
2215	19	-0.5801053717490348
2215	20	-0.5827809472295442
2215	21	-0.5995526470530379
2215	22	-0.5179947613966434
2215	23	-0.6441321690057364
2215	24	-0.6889286613689336
2215	25	-0.6614828776101476
2215	26	-0.697011172555527
2215	27	-0.5537542871768295
2215	28	-0.5077784383523181
2215	29	-0.6387958651893797
2215	30	-0.5437921097790692
2215	31	-0.5995079116537525
2215	32	-0.6966369555141441
2215	33	-0.7035768139567935
2215	34	-0.664958302757455
2215	35	-0.6532468261967495
2215	36	-0.5789217742498636
2215	37	-0.5982182807230672
2215	38	-0.5991482819402663
2215	39	-0.6032135116272883
2215	40	-0.7002283816509838
2215	41	-0.7120596117106146
2215	42	-0.554402407249853
2215	43	-0.6349462046160674
2215	44	-0.7704420919187669
2215	45	-0.6926022380300978
2215	46	-0.7232338129911895
2215	47	-0.6624241348907205
2215	48	-0.800566811560806
2215	49	-0.7880372544752383
2215	50	-0.8182727442955127
2216	1	-0.5735396822511163
2216	2	-0.6239980156285299
2216	3	-0.7906317725630103
2216	4	-0.606131556859328
2216	5	-0.6958231894964726
2216	6	-0.8205893107330944
2216	7	-0.6932689546295592
2216	8	-0.7331901744316873
2216	9	-0.7140360341154519
2216	10	-0.7640538234857743
2216	11	-0.9187578248417907
2216	12	-0.9617114637466457
2216	13	-0.8584493402599662
2216	14	-0.8737421499086437
2216	15	-0.9643055971939702
2216	16	-1.0219138103226442
2216	17	-0.8873571550694166
2216	18	-0.9295877610696337
2216	19	-0.9904296117632327
2216	20	-1.0104128549586093
2216	21	-1.2074717447474868
2216	22	-0.9782882449130668
2216	23	-1.0802510883829086
2216	24	-1.2538252739872178
2216	25	-1.2828057446818733
2216	26	-1.1644528943446417
2216	27	-1.1939442432153697
2216	28	-1.307207765581765
2216	29	-1.4341007324177255
2216	30	-1.2398427628068376
2216	31	-1.3917115238359408
2216	32	-1.3209578518997382
2216	33	-1.4223570145601414
2216	34	-1.4466440979397248
2216	35	-1.5074046721929832
2216	36	-1.530245496267451
2216	37	-1.6637522806267988
2216	38	-1.560064410314336
2216	39	-1.6027788255397013
2216	40	-1.7442707668493576
2216	41	-1.6902966271305324
2216	42	-1.6234515709475588
2216	43	-1.7501392822846675
2216	44	-1.7616549727199171
2216	45	-1.939430281116497
2216	46	-1.9537136818899121
2216	47	-1.8882330394033282
2216	48	-1.9768273070989224
2216	49	-1.9775736079946356
2216	50	-1.9696510678975514
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	214057.0
3	10598.0
4	19310.0
5	14924.0
6	12421.0
7	10834.0
8	9402.0
9	8481.0
10	8298.0
11	8266.0
12	8433.0
13	8937.0
14	9362.0
15	10340.0
16	10701.0
17	11783.0
18	12699.0
19	14134.0
20	17215.0
21	21284.0
22	26075.0
23	29321.0
24	32332.0
25	35739.0
26	39911.0
27	44665.0
28	51207.0
29	58823.0
30	69205.0
31	82973.0
32	100157.0
33	122749.0
34	156777.0
35	202138.0
36	264584.0
37	359908.0
38	555532.0
39	894912.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.469922323736355	16.380784211659964	12.540105812033314	25.609187652570363
2	16.506181912017144	18.54792384243417	38.607022248151594	26.33887199739709
3	20.31006351567078	22.519296920823788	27.353534288167538	29.817105275337894
4	13.684997726464983	14.988893199395331	34.92051603774882	36.40559303639087
5	15.408582139265874	36.15774172240228	33.05962226470081	15.374053873631036
6	35.14826028221522	34.04370337513157	16.39022368721362	14.417812655439594
7	30.909062871498104	30.228786757284166	19.627240203665075	19.234910167552655
8	27.61828762287484	34.13168447810895	18.68978929653542	19.560238602480798
9	27.045908211616254	15.88703339138442	17.853358527747456	39.21369986925187
10	15.675660066746811	28.179949491324706	32.09675484004091	24.04763560188757
11	36.870764243762	21.970266403536833	21.705295520615497	19.45367383208567
12	24.61270152810879	23.560745829244357	28.536015701668305	23.29053694097855
13	29.285009556401835	20.51215697115472	24.58334957141283	25.61948390103061
14	23.093010000053273	20.057554908891486	25.402860530560485	31.446574560494756
15	25.06817222184528	27.709547924104676	22.118444434101868	25.10383541994818
16	25.205028921344596	26.659171136413978	23.771999428021882	24.363800514219545
17	24.098814363343088	26.657773059941842	24.982707654642073	24.260704922072996
18	24.13531865594265	26.38055456020259	25.41520237628604	24.06892440756872
19	25.03151016919734	25.986138609974624	25.37399311164568	23.60835810918235
20	25.19740791953547	25.469750788888568	25.352143087687278	23.980698203888682
21	25.395444528391632	25.24997658266152	25.277151913182532	24.077426975764315
22	25.158352766633524	24.978327721865654	25.12267869638197	24.74064081511885
23	24.844012383116183	25.0851472422724	25.21833219355054	24.852508181060877
24	24.59685448060678	25.21755078895989	25.248371068311087	24.93722366212224
25	24.862048279105746	25.05904403769507	25.271465147540678	24.807442535658502
26	24.646266630768885	25.449563617598887	25.425248479953332	24.478921271678892
27	24.665418350804053	25.45389331246139	25.262215038065033	24.618473298669524
28	24.726651764965258	25.46224679472705	25.175750412115537	24.635351028192154
29	24.746344548479694	25.594849624112868	25.171803066274208	24.48700276113323
30	24.902943838255343	25.521697026602492	25.129321744085836	24.446037391056333
31	24.958139169736835	25.031711050922734	25.078478542469256	24.931671236871175
32	24.58565867184795	25.6366065408075	25.116908277574275	24.660826509770274
33	24.88998400965421	25.189799488409875	25.046803234631337	24.873413267304578
34	24.934646350120616	25.2987875790496	25.125240134710946	24.64132593611884
35	24.960549691126793	25.35415554468904	25.132342187549067	24.552952576635096
36	24.879525791308136	25.39621834213694	25.044391875466665	24.679863991088258
37	24.96597445406313	25.07583196455399	25.2647880041655	24.69340557721738
38	24.99547214457737	25.286824921989115	25.1247753594949	24.592927573938617
39	24.96514931122927	25.501563935172673	25.0581218646997	24.47516488889836
40	25.070397340167965	25.160092089225948	25.200747727279737	24.56876284332635
41	24.77873600436893	25.406545433341016	25.169007285101287	24.645711277188767
42	24.986515641855448	25.333184192407494	25.07860736016076	24.6016928055763
43	24.767880751258993	25.007372869857353	25.162651676472862	25.062094702410786
44	24.83037792999822	25.358860393109993	25.074323102605828	24.73643857428596
45	24.932445387281344	25.283140723266033	24.926839195354486	24.857574694098137
46	24.954478997551284	25.069683012369154	25.003391651044517	24.972446339035045
47	24.842384636149653	25.239117595035243	25.09481029111784	24.823687477697263
48	24.702832353075554	25.369611326833535	25.10946437646159	24.818091943629312
49	25.022522004853652	25.124438631671183	25.012094891218855	24.84094447225631
50	24.969909422737434	25.208699946910524	25.103950541013724	24.71744008933832
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1780.0
1	1289.5
2	799.0
3	781.0
4	763.0
5	750.0
6	737.0
7	725.5
8	714.0
9	785.0
10	856.0
11	1065.5
12	1275.0
13	1861.5
14	2448.0
15	3204.0
16	3960.0
17	4324.5
18	4689.0
19	4733.5
20	4778.0
21	5163.5
22	5549.0
23	6871.5
24	8194.0
25	10478.5
26	12763.0
27	16688.0
28	20613.0
29	26700.5
30	32788.0
31	41777.5
32	50767.0
33	63119.0
34	75471.0
35	90465.0
36	105459.0
37	121687.0
38	137915.0
39	153552.0
40	169189.0
41	185659.5
42	202130.0
43	214759.0
44	227388.0
45	242220.0
46	257052.0
47	268544.0
48	280036.0
49	284782.5
50	289529.0
51	290216.0
52	290903.0
53	286027.5
54	281152.0
55	270456.5
56	259761.0
57	243279.5
58	226798.0
59	207038.5
60	187279.0
61	165027.0
62	142775.0
63	122466.5
64	102158.0
65	85167.5
66	68177.0
67	56403.5
68	44630.0
69	36354.0
70	28078.0
71	22058.5
72	16039.0
73	13144.5
74	10250.0
75	7978.0
76	5706.0
77	4441.0
78	3176.0
79	2495.0
80	1814.0
81	1367.5
82	921.0
83	727.0
84	533.0
85	410.0
86	287.0
87	230.0
88	173.0
89	132.0
90	91.0
91	70.0
92	49.0
93	41.0
94	33.0
95	23.0
96	13.0
97	13.0
98	13.0
99	24.5
100	36.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08179937323577191
2	0.09099094378093574
3	0.09920170649353632
4	0.09959402962656162
5	0.09225198242280272
6	0.055737907970520834
7	0.05175863047840723
8	0.057531385150065
9	0.05851219298262821
10	0.04413635246534456
11	0.04707877596303419
12	0.05568186180866008
13	0.04954480708490741
14	0.050609684160261764
15	0.050021199460723836
16	0.05372024614353366
17	0.05268339214910969
18	0.05360815381981215
19	0.06103427026636219
20	0.06708725574732373
21	0.07714754180132925
22	0.08048228843204416
23	0.05655057731750178
24	0.0743452337082915
25	0.08205158096414532
26	0.07866078817156963
27	0.07330837971386753
28	0.0937652287930431
29	0.08188344247856305
30	0.0775678880152849
31	0.05344001533422988
32	0.05049759183654025
33	0.05492523862353989
34	0.048732137737926465
35	0.055345584837495555
36	0.054813146299818385
37	0.05741929282634349
38	0.04699470672024306
39	0.05164653815468573
40	0.05453291549051461
41	0.04150218285788907
42	0.0389801055741551
43	0.037971274660661504
44	0.03620582056204773
45	0.02810715017316863
46	0.025304842080130882
47	0.030825389023415244
48	0.024884495866175214
49	0.024239965004776536
50	0.025921349860599182
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3568487.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.710486895254554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.7088261371716	47.177406708463714
2	12.226174167414955	14.845139731398907
3	4.348787638644946	7.920510448385971
4	1.9350362354253086	4.6990796805052355
5	1.050054363048442	3.187465582357866
6	0.6590288369778435	2.400597694256293
7	0.42397305994427	1.8017727629781308
8	0.28424476290511375	1.3805310362716519
9	0.22054917594058598	1.2050683070130124
>10	1.0725267214456249	11.297974976245108
>50	0.05393175917920706	2.2271026791663147
>100	0.016124003870849685	1.5184426007672673
>500	6.502457787716258E-4	0.26333803818091717
>1k	9.289225255338232E-5	0.07556975400959143
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8023080930377497E-5
2	8.406924279113249E-5	0.0	0.0	0.0	2.8023080930377497E-5
3	8.406924279113249E-5	0.0	0.0	0.0	2.8023080930377497E-5
4	8.406924279113249E-5	0.0	0.0	0.0	2.8023080930377497E-5
5	8.406924279113249E-5	0.0	0.0	0.0	2.8023080930377497E-5
6	1.1209232372150999E-4	0.0	0.0	0.0	2.8023080930377497E-5
7	1.6813848558226498E-4	0.0	0.0	0.0	2.8023080930377497E-5
8	1.9616156651264247E-4	0.0	0.0	0.0	2.8023080930377497E-5
9	1.9616156651264247E-4	0.0	0.0	0.0	8.406924279113249E-5
10	2.2418464744301998E-4	0.0	0.0	0.0	8.406924279113249E-5
11	2.2418464744301998E-4	0.0	0.0	0.0	8.406924279113249E-5
12	2.5220772837339746E-4	0.0	0.0	0.0	1.1209232372150999E-4
13	3.0825389023415243E-4	0.0	0.0	0.0	1.4011540465188747E-4
14	4.203462139556624E-4	0.0	0.0	5.6046161860754995E-5	1.4011540465188747E-4
15	4.203462139556624E-4	0.0	0.0	5.6046161860754995E-5	1.6813848558226498E-4
16	4.203462139556624E-4	0.0	0.0	5.6046161860754995E-5	1.6813848558226498E-4
17	4.4836929488603996E-4	0.0	0.0	8.406924279113249E-5	1.6813848558226498E-4
18	4.4836929488603996E-4	0.0	0.0	8.406924279113249E-5	2.5220772837339746E-4
19	5.604616186075499E-4	0.0	0.0	8.406924279113249E-5	2.5220772837339746E-4
20	5.604616186075499E-4	0.0	0.0	8.406924279113249E-5	2.5220772837339746E-4
21	5.604616186075499E-4	0.0	0.0	8.406924279113249E-5	2.8023080930377495E-4
22	5.604616186075499E-4	0.0	0.0	1.4011540465188747E-4	2.8023080930377495E-4
23	5.604616186075499E-4	0.0	0.0	1.4011540465188747E-4	3.0825389023415243E-4
24	7.566231851201924E-4	0.0	0.0	2.8023080930377495E-4	3.3627697116452997E-4
25	7.566231851201924E-4	0.0	0.0	3.3627697116452997E-4	3.6430005209490745E-4
26	7.566231851201924E-4	0.0	0.0	6.165077804683049E-4	4.203462139556624E-4
27	7.566231851201924E-4	0.0	0.0	7.566231851201924E-4	4.4836929488603996E-4
28	7.846462660505699E-4	0.0	0.0	0.0013170848037277424	4.4836929488603996E-4
29	9.527847516328349E-4	0.0	0.0	0.0022418464744301996	4.4836929488603996E-4
30	9.527847516328349E-4	0.0	0.0	0.004175439058626247	4.4836929488603996E-4
31	9.527847516328349E-4	0.0	0.0	0.009780055244701746	4.4836929488603996E-4
32	9.527847516328349E-4	0.0	0.0	0.016141294615897438	4.4836929488603996E-4
33	0.0010088309134935899	0.0	0.0	0.024492172733149933	4.4836929488603996E-4
34	0.0010088309134935899	0.0	0.0	0.03418815873506054	5.324385376771724E-4
35	0.0010929001562847223	0.0	0.0	0.047190868286755706	5.324385376771724E-4
36	0.0011489463181454773	0.0	0.0	0.06246344739381144	5.324385376771724E-4
37	0.0011489463181454773	0.0	0.0	0.08401319662927173	5.604616186075499E-4
38	0.0013451078846581199	0.0	0.0	0.10659979985915599	5.604616186075499E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5115	0.0	18.801607	1
GGTATCA	3060	0.0	16.39731	1
TAGCGCG	130	1.3689047E-5	15.232566	29
TATCAAC	6320	0.0	14.938239	2
TCAACGC	6255	0.0	14.636094	4
ATCAACG	6335	0.0	14.59022	3
GTACATG	9765	0.0	14.490984	1
CAACGCA	6375	0.0	14.392892	5
CGCGCAA	110	0.0010170203	14.003026	21
AACGCAG	6590	0.0	13.9518175	6
TACATGG	9960	0.0	13.875846	2
GTATAGA	545	0.0	13.32529	1
ACATGGG	10410	0.0	13.022344	3
GTATAGC	745	0.0	12.111189	1
GTGTAGC	1185	0.0	12.071304	1
GTATTGG	740	0.0	11.89563	1
GTGTACG	390	0.0	11.849877	1
TCTATAC	465	0.0	11.831676	3
ACGCAGA	7605	0.0	11.800347	7
TACACTG	1815	0.0	11.759457	5
>>END_MODULE