FastQCFastQC Report
Mon 6 Feb 2017
SRR2077129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568487
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1051.7920083E-618.8600862
GTATCAA50700.018.5320131
GGTATCA30350.017.6902431
GTACCGA3200.015.8145266
TGCGATA3000.015.40413113
GTACATG93400.014.4180711
ACCGTCG1554.5623165E-614.1965618
GTTATCG1554.5882207E-614.18880343
GCGATAC3150.013.97180614
TACATGG95050.013.7275512
TATCAAC68600.013.6960152
TCAACGC69050.013.6386234
TAGACTG6700.013.4655445
CAACGCA69350.013.4534675
ATCAACG69600.013.4042023
CGAATCG2805.820766E-1113.35398637
GGACCGT3350.013.1360116
GTATTAG5250.012.9928691
TATATCG1200.00195981512.8360575
GTATAAG7050.012.7966821
Produced by FastQC (version 0.11.5)