FastQCFastQC Report
Mon 6 Feb 2017
SRR2077128_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077128_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020280
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA951.601333E-518.52266310
GTATCAA64200.017.7229421
TGCGATA2450.016.1616913
CGCACGA2403.6379788E-1215.579679535
GGTATCA38250.015.0747531
GTACATG121900.014.9849291
TACATGG123550.014.4276492
TATCAAC79650.014.1461252
GCGATAC2803.6379788E-1214.14235714
ACATGGG127850.013.9773483
TCAACGC80250.013.7111174
ATCAACG81700.013.683943
CAACGCA80600.013.6507285
CGTATAC1000.008745586513.2044633
AACGCAG83300.013.2043236
GTACCGT3051.8189894E-1112.9826626
TATACAG9400.012.6411555
CATGGGG87700.012.3205424
CGCACTC6200.012.06063843
GCGCACT5850.012.03091842
Produced by FastQC (version 0.11.5)