FastQCFastQC Report
Mon 6 Feb 2017
SRR2077128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4020280
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74550.019.5064371
GGTATCA41650.016.9556391
CGCGTAA800.001986743916.5033410
GACCGTA1603.8232247E-715.1273097
TATCAAC98100.014.7349112
CGCGATC1351.9563095E-514.66817723
CAACGCA98900.014.4829665
ATCAACG99700.014.4324213
TCAACGC99900.014.42554954
GTACATG116900.014.1899551
ACCGTCG2358.221832E-1014.0448748
GGACCGT3450.014.0300816
CTAGTAC3000.013.934423
CGTCGTA1901.7516868E-713.89755110
AACGCAG103100.013.8711116
GCGATAC1751.0518052E-613.83154414
TAGATCG1453.835986E-513.6567495
TACATGG119000.013.5521492
TATACTG7850.013.4537815
TACCGAT3650.013.26237
Produced by FastQC (version 0.11.5)