##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077128_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4020280
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.44686166137682	31.0	31.0	34.0	27.0	34.0
2	30.560086859621716	31.0	31.0	34.0	27.0	34.0
3	30.614845234660272	31.0	31.0	34.0	27.0	34.0
4	34.15283064861154	37.0	35.0	37.0	30.0	37.0
5	33.66177803536072	37.0	35.0	37.0	28.0	37.0
6	33.578544777975665	37.0	35.0	37.0	28.0	37.0
7	33.4075726068831	37.0	35.0	37.0	27.0	37.0
8	33.332407941735404	37.0	35.0	37.0	27.0	37.0
9	34.75717213726407	39.0	35.0	39.0	27.0	39.0
10	34.61489448496125	39.0	35.0	39.0	27.0	39.0
11	34.61716074502274	39.0	35.0	39.0	27.0	39.0
12	34.52559572965067	39.0	35.0	39.0	27.0	39.0
13	34.49913016008835	39.0	35.0	39.0	27.0	39.0
14	35.54481478901967	40.0	36.0	41.0	26.0	41.0
15	35.55169465808352	40.0	36.0	41.0	27.0	41.0
16	35.52010059000865	40.0	36.0	41.0	26.0	41.0
17	35.495760743032825	40.0	36.0	41.0	26.0	41.0
18	35.41828181121713	40.0	36.0	41.0	25.0	41.0
19	35.471974837573505	40.0	35.0	41.0	26.0	41.0
20	35.43458067597282	40.0	35.0	41.0	25.0	41.0
21	35.40638164505955	40.0	35.0	41.0	25.0	41.0
22	35.31231630632692	39.0	35.0	41.0	25.0	41.0
23	35.26369481727641	39.0	35.0	41.0	25.0	41.0
24	35.250755171281604	39.0	35.0	41.0	25.0	41.0
25	35.17758116350105	39.0	35.0	41.0	25.0	41.0
26	35.11331076442437	39.0	35.0	41.0	24.0	41.0
27	35.00755022038266	39.0	35.0	41.0	24.0	41.0
28	34.97165048205598	39.0	35.0	41.0	24.0	41.0
29	34.93909454067876	39.0	35.0	41.0	24.0	41.0
30	34.86230262568776	39.0	35.0	41.0	24.0	41.0
31	34.78004094242192	39.0	34.0	41.0	23.0	41.0
32	34.70910384351339	39.0	34.0	41.0	23.0	41.0
33	34.58911891709035	39.0	34.0	41.0	22.0	41.0
34	34.54640248937885	39.0	34.0	41.0	22.0	41.0
35	34.48001681474922	39.0	34.0	41.0	21.0	41.0
36	34.403223407324866	39.0	34.0	41.0	20.0	41.0
37	34.31318465380521	39.0	34.0	41.0	19.0	41.0
38	34.14803670391117	39.0	34.0	41.0	18.0	41.0
39	33.998043419861304	39.0	33.0	41.0	16.0	41.0
40	33.97504925030098	39.0	33.0	41.0	16.0	41.0
41	33.86524097824032	38.0	33.0	41.0	15.0	41.0
42	33.99786407911887	39.0	33.0	41.0	15.0	41.0
43	33.979125334553814	39.0	33.0	41.0	15.0	41.0
44	33.89912220044375	39.0	33.0	41.0	14.0	41.0
45	33.7613410011243	39.0	33.0	41.0	9.0	41.0
46	33.64694150656173	39.0	33.0	41.0	7.0	41.0
47	33.50804123095904	38.0	33.0	41.0	2.0	41.0
48	33.36257773090431	38.0	33.0	41.0	2.0	41.0
49	33.10907275115166	38.0	33.0	41.0	2.0	41.0
50	32.9839466405325	38.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39044402741888007
1101	2	0.5877818514767519
1101	3	0.44435064513711353
1101	4	0.39611338672573027
1101	5	0.6805949456373241
1101	6	0.6510110605455068
1101	7	0.7972610078736011
1101	8	0.8007730014099579
1101	9	0.9262524686455293
1101	10	1.0606693104726972
1101	11	0.9791039895245319
1101	12	0.9310008840967896
1101	13	1.0424027281476143
1101	14	1.0652628064928749
1101	15	1.135029555259493
1101	16	1.0806551227338232
1101	17	0.9951734550297004
1101	18	1.1778471980917757
1101	19	1.1896917206895878
1101	20	1.1674808355831843
1101	21	1.1925487130569863
1101	22	1.3038167506374734
1101	23	1.2363686475814433
1101	24	1.3192430812435987
1101	25	1.3092184082450729
1101	26	1.3085517413392012
1101	27	1.4897890521622514
1101	28	1.439079209091851
1101	29	1.3917251833215474
1101	30	1.344266881675452
1101	31	1.2695985177185278
1101	32	1.4148179261052647
1101	33	1.4645445566058157
1101	34	1.4941446230714561
1101	35	1.429754167660576
1101	36	1.5450024287364599
1101	37	1.5561829428537592
1101	38	1.5553519107166665
1101	39	1.6570544236909086
1101	40	1.649501370020765
1101	41	1.6807143564892044
1101	42	1.602614986874869
1101	43	1.636127523732874
1101	44	1.6256205915205157
1101	45	1.6910669801698148
1101	46	1.6549962699246876
1101	47	1.7832281355417052
1101	48	1.8145481622710378
1101	49	1.9021655545172251
1101	50	1.9102279776748006
1102	1	0.28941156185697636
1102	2	0.39590311910223264
1102	3	0.328068957209684
1102	4	0.3093293794425236
1102	5	0.394320871482968
1102	6	0.4704009832697764
1102	7	0.5832343244439926
1102	8	0.5389234235543867
1102	9	0.6095951046909178
1102	10	0.6355729867599891
1102	11	0.5441807504466354
1102	12	0.6717883541565115
1102	13	0.6833291969826263
1102	14	0.7625610008635633
1102	15	0.8604201336894803
1102	16	0.7090478579479296
1102	17	0.6730492123427183
1102	18	0.7384922553711917
1102	19	0.6865924273344319
1102	20	0.7801005967112147
1102	21	0.7947821768089227
1102	22	0.831129603553471
1102	23	0.8891777505109815
1102	24	0.7677609695460248
1102	25	0.8303221072924032
1102	26	0.8038733351918239
1102	27	0.8183339273614081
1102	28	0.8081259701223402
1102	29	0.8107051544921688
1102	30	0.7655666150579208
1102	31	0.7141961257473994
1102	32	0.8509557110121122
1102	33	0.874877611760958
1102	34	0.8828605236643057
1102	35	0.8184875447076223
1102	36	0.8830844353043545
1102	37	0.8942331124701965
1102	38	1.0057901768356459
1102	39	0.9373877227035621
1102	40	0.9360313590742706
1102	41	0.9598374449173548
1102	42	0.9724196028263989
1102	43	0.9063532003019574
1102	44	0.93980361470323
1102	45	1.0315450221566564
1102	46	1.025331953321789
1102	47	0.994978273727618
1102	48	1.0861536658638755
1102	49	1.1927183794708967
1102	50	1.1248634919973597
1103	1	0.10755491747847401
1103	2	0.17244675307042812
1103	3	0.1251541239938021
1103	4	0.10312279009700376
1103	5	0.1926176772129935
1103	6	0.22311138721423873
1103	7	0.2847132622484665
1103	8	0.29990839613216735
1103	9	0.3144623420350996
1103	10	0.39909180403398636
1103	11	0.3592646492330829
1103	12	0.33242235888440064
1103	13	0.3251800434535639
1103	14	0.47442004925252235
1103	15	0.4199580157091347
1103	16	0.4364196087039005
1103	17	0.4922776133693745
1103	18	0.4775884476071752
1103	19	0.4484701539418836
1103	20	0.3956479745721637
1103	21	0.42168594097078227
1103	22	0.46041098119287227
1103	23	0.5301306872925764
1103	24	0.4028597578776214
1103	25	0.4415408816713082
1103	26	0.4007708948855253
1103	27	0.4957273908098401
1103	28	0.47478767753541007
1103	29	0.42473886013287654
1103	30	0.4874415487708674
1103	31	0.5162826948709522
1103	32	0.5102713104474077
1103	33	0.5967937023685863
1103	34	0.6131641360435438
1103	35	0.47771797264505267
1103	36	0.5120242136640201
1103	37	0.5079862323808229
1103	38	0.420424502098264
1103	39	0.6073623097204859
1103	40	0.52494471688766
1103	41	0.5426161274724848
1103	42	0.494298307536134
1103	43	0.4788934792614512
1103	44	0.49317400200817474
1103	45	0.4797949555749952
1103	46	0.471081774788658
1103	47	0.4525209743741101
1103	48	0.437126170583511
1103	49	0.5059214936071825
1103	50	0.5078676683763064
1104	1	0.31703035242798094
1104	2	0.31882096120033054
1104	3	0.26683956898708416
1104	4	0.2647464778964306
1104	5	0.31287418676106427
1104	6	0.34600787255456567
1104	7	0.2428794927275746
1104	8	0.34426298363785435
1104	9	0.4014934965403043
1104	10	0.43531925971613816
1104	11	0.3344647526319662
1104	12	0.40003272251269806
1104	13	0.41048015629509393
1104	14	0.493801315728696
1104	15	0.45004607378636763
1104	16	0.5063285894738172
1104	17	0.4889225382266247
1104	18	0.5473696539960997
1104	19	0.4691161758236575
1104	20	0.5038435687579863
1104	21	0.5436889969313583
1104	22	0.53224427921689
1104	23	0.5601408911965464
1104	24	0.5700659686224867
1104	25	0.6137835177734559
1104	26	0.48046410928303374
1104	27	0.5422037142322935
1104	28	0.5424673041810593
1104	29	0.5111569670360865
1104	30	0.48456361321038344
1104	31	0.519948729189565
1104	32	0.5267517655041587
1104	33	0.4955138870225184
1104	34	0.6152568958723137
1104	35	0.5779252660534908
1104	36	0.5508839944344928
1104	37	0.540840846003114
1104	38	0.5951198623784251
1104	39	0.7606038761220546
1104	40	0.7191307156673616
1104	41	0.6816209030622176
1104	42	0.6903784782713842
1104	43	0.7517780755095274
1104	44	0.7441000104236934
1104	45	0.7712462089623742
1104	46	0.7579785463313939
1104	47	0.7431825276932287
1104	48	0.7418688063679397
1104	49	0.8694581115664874
1104	50	0.9259913217984064
1105	1	0.1387682486281996
1105	2	0.23175518357493274
1105	3	0.19746337757919008
1105	4	0.10379742046603724
1105	5	0.1127879339622524
1105	6	0.0980251029947965
1105	7	0.0778525554136138
1105	8	0.05326809939030852
1105	9	0.1715291175339786
1105	10	0.2102047265282181
1105	11	0.18059486682165016
1105	12	0.2795755672024498
1105	13	0.19882510297242106
1105	14	0.28819854014323454
1105	15	0.21225507789856124
1105	16	0.3100774764008918
1105	17	0.25536305125424263
1105	18	0.2311556682213336
1105	19	0.2677252974512996
1105	20	0.2909449980883494
1105	21	0.18234113275335062
1105	22	0.36543117770016664
1105	23	0.31547034711980615
1105	24	0.27011898309227433
1105	25	0.34095564741159734
1105	26	0.2779798361876331
1105	27	0.21727804584867272
1105	28	0.22644580811093107
1105	29	0.1890539328590748
1105	30	0.24458304693227007
1105	31	0.14139719438765752
1105	32	0.35213040133784546
1105	33	0.33098483545339263
1105	34	0.38691450863095866
1105	35	0.35862464740947075
1105	36	0.3720030217250141
1105	37	0.40223350756527765
1105	38	0.37534660412671883
1105	39	0.5878156620248518
1105	40	0.4579485691648486
1105	41	0.4117395519266793
1105	42	0.4835517486770229
1105	43	0.404337447399179
1105	44	0.42217473903799885
1105	45	0.38022912794347974
1105	46	0.42931574836924113
1105	47	0.31190607985567453
1105	48	0.3897731919919494
1105	49	0.5535752032472772
1105	50	0.5284661783748348
1106	1	0.21410881408248272
1106	2	0.20531890568492628
1106	3	0.22808961110707315
1106	4	0.15591790043443865
1106	5	0.20479792925605977
1106	6	0.24863794657422034
1106	7	0.26649616137883214
1106	8	0.14508351099570405
1106	9	0.21757751635450262
1106	10	0.16366937569150508
1106	11	0.1369930551658456
1106	12	0.2871114611698289
1106	13	0.3276970209346999
1106	14	0.2677328979977034
1106	15	0.3100127543271469
1106	16	0.36170537240843714
1106	17	0.3872533395640545
1106	18	0.3016410045733906
1106	19	0.25472479796820835
1106	20	0.2903136570906426
1106	21	0.27586971152859263
1106	22	0.4331076520464876
1106	23	0.32699386911272654
1106	24	0.3421839391261443
1106	25	0.272182915034918
1106	26	0.359644723511785
1106	27	0.4159420781732166
1106	28	0.3841340637815094
1106	29	0.31119356495933204
1106	30	0.2851519859830134
1106	31	0.3455770125251263
1106	32	0.3088271531489539
1106	33	0.3876081959855
1106	34	0.36943576834210035
1106	35	0.4594675735285634
1106	36	0.3923751031896856
1106	37	0.32975798276483204
1106	38	0.39506024085994795
1106	39	0.4740816369479077
1106	40	0.5105926791891093
1106	41	0.5008987272269465
1106	42	0.44978718438010645
1106	43	0.4006022712935504
1106	44	0.45636303677216006
1106	45	0.4915269145540222
1106	46	0.4114496710855846
1106	47	0.5022178678326696
1106	48	0.49278209363249914
1106	49	0.5844830340087483
1106	50	0.4777529805812861
1107	1	0.10454287412214214
1107	2	0.12692890604038354
1107	3	0.14950824721140066
1107	4	0.012657983611461532
1107	5	0.05476660821921797
1107	6	-0.032694540654077286
1107	7	0.021294223728375528
1107	8	0.019217229388409862
1107	9	-0.032490690666186595
1107	10	0.12856557340504082
1107	11	0.10949837100135085
1107	12	0.013241363931847161
1107	13	0.06165292907286357
1107	14	0.053632429434991025
1107	15	0.10896805935325204
1107	16	0.12478057960802147
1107	17	0.08830252145857287
1107	18	0.22108120003996845
1107	19	0.1535595388543669
1107	20	0.14284186204851324
1107	21	0.13047658444210697
1107	22	0.2004062752611162
1107	23	0.13425090964476993
1107	24	0.12179816884133032
1107	25	0.07251937581020229
1107	26	0.0791845635792825
1107	27	0.12089313286178793
1107	28	0.09708057793949365
1107	29	0.1115209353499651
1107	30	0.18661652852980382
1107	31	0.09484637285923014
1107	32	0.027671462247674583
1107	33	0.05308343954661865
1107	34	0.06950167363078208
1107	35	0.0895787734068918
1107	36	0.004082120092554931
1107	37	-0.02910336497792798
1107	38	0.11015068727358823
1107	39	0.13755629662663438
1107	40	0.055236898432873716
1107	41	0.05346008878009201
1107	42	0.021258294147081358
1107	43	0.05219614787059612
1107	44	0.0720080549208788
1107	45	0.09174345217801516
1107	46	0.021458650754006214
1107	47	0.034237873671493446
1107	48	-0.006911222429103248
1107	49	0.10212636344370196
1107	50	-0.023441414380776848
1108	1	-0.0079923045094219
1108	2	0.05593022916830037
1108	3	-0.0033526537659653854
1108	4	-0.04036918571521397
1108	5	2.374196576937493E-5
1108	6	-0.1257900812737347
1108	7	-0.13745749002789864
1108	8	0.01200017435553491
1108	9	-0.024372105123163124
1108	10	-0.05973595845311053
1108	11	-0.06581951786008489
1108	12	-0.13914171890274218
1108	13	-0.13223737146751802
1108	14	-0.10046813990744141
1108	15	-0.13635303448516822
1108	16	-0.16350328885265242
1108	17	-0.08274926181247366
1108	18	-0.04026628942184374
1108	19	-0.02911448105367498
1108	20	-0.13025441299661367
1108	21	-0.0297377980722473
1108	22	-0.0743675577170535
1108	23	0.012064613326863594
1108	24	0.006088296652279723
1108	25	-0.15478454130384023
1108	26	-0.04395090793929768
1108	27	-0.03664673238043292
1108	28	-0.17702297558745528
1108	29	-0.15837914792365382
1108	30	-0.11368621280624325
1108	31	-0.14480009888874434
1108	32	-0.1740734257675456
1108	33	-0.17810786597135575
1108	34	-0.19017323153885002
1108	35	-0.16315911130364213
1108	36	-0.18024166113882956
1108	37	-0.18283400235000613
1108	38	-0.1043171924191526
1108	39	0.005365614673074504
1108	40	-0.11289730992037761
1108	41	-0.16347255533986527
1108	42	-0.0887021654080371
1108	43	-0.1716361911606512
1108	44	-0.32464603277277604
1108	45	-0.2031023452000369
1108	46	-0.1058710874581763
1108	47	-0.13681997558209957
1108	48	-0.21927506610524716
1108	49	-0.09001553964327513
1108	50	-0.2500835118813356
1109	1	0.07633750203045153
1109	2	0.010116035625397757
1109	3	0.008089092243004359
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2211	6	-0.27342523473758007
2211	7	-0.3379251874097662
2211	8	-0.31042389034698914
2211	9	-0.22677647393085465
2211	10	-0.2912908239045535
2211	11	-0.3067128333654736
2211	12	-0.34474435857385544
2211	13	-0.3371145319439677
2211	14	-0.24414248188394083
2211	15	-0.31097479158149355
2211	16	-0.3612359488995409
2211	17	-0.4267876024218893
2211	18	-0.33532283499904736
2211	19	-0.3283994175170193
2211	20	-0.411593628544189
2211	21	-0.3969094982487178
2211	22	-0.25037246841365857
2211	23	-0.18759995670217933
2211	24	-0.24189073376670933
2211	25	-0.3526205796178985
2211	26	-0.209880374934599
2211	27	-0.3187670078207958
2211	28	-0.32829850453692444
2211	29	-0.2537851788872345
2211	30	-0.23144227718005084
2211	31	-0.23060232088052146
2211	32	-0.23511927686759293
2211	33	-0.2078499262068263
2211	34	-0.14529442744868248
2211	35	-0.18683795376006174
2211	36	-0.15700064258361834
2211	37	-0.20587366677810337
2211	38	-0.12539163965102773
2211	39	-0.059398397420075355
2211	40	-0.08809321013425375
2211	41	-0.10355981337325915
2211	42	-0.16654174275665667
2211	43	-0.18226263461633607
2211	44	-0.38315085247544545
2211	45	-0.254109676678695
2211	46	-0.27667357155826977
2211	47	-0.2695862621802618
2211	48	-0.27208851846551596
2211	49	-0.1263943655948836
2211	50	-0.2661362842433519
2212	1	-0.25054532143425945
2212	2	-0.2581688481704205
2212	3	-0.21073068390204952
2212	4	-0.29759706430062494
2212	5	-0.2654116350463198
2212	6	-0.32843207662694596
2212	7	-0.3750151373971775
2212	8	-0.32885086388687057
2212	9	-0.34559837214276
2212	10	-0.3212581537288628
2212	11	-0.33078125517078405
2212	12	-0.3309465091635104
2212	13	-0.4498531007701061
2212	14	-0.5237366518483668
2212	15	-0.5464040533663237
2212	16	-0.4342414337217875
2212	17	-0.46326267266898213
2212	18	-0.5064958760623597
2212	19	-0.500911966020297
2212	20	-0.5096375951445253
2212	21	-0.4927554595966086
2212	22	-0.5025523596534356
2212	23	-0.47231498884657697
2212	24	-0.47472812951034626
2212	25	-0.4037085906500266
2212	26	-0.37548957521801896
2212	27	-0.4015063975168687
2212	28	-0.511007019220358
2212	29	-0.5202604137562616
2212	30	-0.5280941917052004
2212	31	-0.5038462051474966
2212	32	-0.46937056229056395
2212	33	-0.3511597298016511
2212	34	-0.4605797312674298
2212	35	-0.5079793638936749
2212	36	-0.48504829022009943
2212	37	-0.45383193478060235
2212	38	-0.35963217425349114
2212	39	-0.18379051971155747
2212	40	-0.2595250772012534
2212	41	-0.2928252534832936
2212	42	-0.4484961881705445
2212	43	-0.5210800510799416
2212	44	-0.35999430182693004
2212	45	-0.32273751698107844
2212	46	-0.3584184431649149
2212	47	-0.47018329107902446
2212	48	-0.543223091749212
2212	49	-0.27766902654928316
2212	50	-0.312721202118837
2213	1	-0.3443136110427183
2213	2	-0.26625750749660426
2213	3	-0.4020478513469783
2213	4	-0.3309621832624643
2213	5	-0.3544035635770726
2213	6	-0.38554534754337055
2213	7	-0.4009233604297293
2213	8	-0.3955613410419261
2213	9	-0.4311348486215678
2213	10	-0.4794561611906545
2213	11	-0.47758896782559646
2213	12	-0.48290328798118054
2213	13	-0.502437646123326
2213	14	-0.41862007024316483
2213	15	-0.438015700971377
2213	16	-0.42532834350462423
2213	17	-0.5030258769643439
2213	18	-0.47902610523228617
2213	19	-0.596145704394246
2213	20	-0.6206671396842935
2213	21	-0.655977754250145
2213	22	-0.5377673458626973
2213	23	-0.534664662264106
2213	24	-0.4838484842599584
2213	25	-0.44493664015799794
2213	26	-0.4635002161683843
2213	27	-0.47419278593731207
2213	28	-0.47387432194831547
2213	29	-0.5385164356157901
2213	30	-0.5190654875792973
2213	31	-0.563595415063233
2213	32	-0.5089275526825858
2213	33	-0.4799969905281358
2213	34	-0.41327569248374374
2213	35	-0.5531189949601298
2213	36	-0.478728979715342
2213	37	-0.47088793702242526
2213	38	-0.4561788413427976
2213	39	-0.3901492086891736
2213	40	-0.5030259861742579
2213	41	-0.52380769317908
2213	42	-0.5035573122068797
2213	43	-0.6324742980406342
2213	44	-0.5767227894472384
2213	45	-0.6532909825795272
2213	46	-0.7029300752161873
2213	47	-0.6235001651525423
2213	48	-0.6812828864938396
2213	49	-0.6240102956493274
2213	50	-0.6795813022701722
2214	1	-0.09600352904461218
2214	2	-0.16065510054681553
2214	3	-0.1137272946468535
2214	4	-0.0659323408116208
2214	5	-0.10727910307559085
2214	6	-0.1603753417481002
2214	7	-0.24571064640149132
2214	8	-0.10700946948153955
2214	9	-0.24467957350920244
2214	10	-0.22073872593047383
2214	11	-0.19235290445737263
2214	12	-0.25624244374216687
2214	13	-0.25060169511814934
2214	14	-0.24295618242484807
2214	15	-0.40119311167605076
2214	16	-0.33723236236121323
2214	17	-0.28867559719026303
2214	18	-0.39266268301926033
2214	19	-0.3384798573071848
2214	20	-0.4162832802897256
2214	21	-0.446639931117808
2214	22	-0.3370134117389014
2214	23	-0.2639243006194434
2214	24	-0.33699804153819457
2214	25	-0.29092570955670993
2214	26	-0.3618162138757768
2214	27	-0.28722958342026317
2214	28	-0.3763287922408267
2214	29	-0.3107059689194571
2214	30	-0.20596776183300136
2214	31	-0.37391979784575824
2214	32	-0.3857696142258007
2214	33	-0.3560920166564472
2214	34	-0.29277353555237795
2214	35	-0.3547691147620924
2214	36	-0.34987925219220983
2214	37	-0.3775064290337369
2214	38	-0.38390698403679124
2214	39	-0.41412793409824644
2214	40	-0.46264551396604503
2214	41	-0.47321288100816616
2214	42	-0.46086665810654637
2214	43	-0.4122243823483487
2214	44	-0.3158485113763092
2214	45	-0.46708964902987304
2214	46	-0.5128650218968716
2214	47	-0.5537261895266852
2214	48	-0.47415716669016916
2214	49	-0.4082411755101276
2214	50	-0.41407094127729493
2215	1	-0.5060598968275123
2215	2	-0.5280750283580957
2215	3	-0.44899501837947753
2215	4	-0.4409379396154094
2215	5	-0.48952380027811415
2215	6	-0.5437349754438188
2215	7	-0.6324417115568721
2215	8	-0.6765911596139063
2215	9	-0.7300606763381197
2215	10	-0.7248463890242931
2215	11	-0.7753776544453359
2215	12	-0.8198265757496515
2215	13	-0.8746238076047987
2215	14	-0.9219310852667917
2215	15	-0.9396678036666017
2215	16	-0.8693212977440297
2215	17	-0.9339130156823003
2215	18	-0.8851181068040077
2215	19	-0.9226446388219429
2215	20	-1.04591953242479
2215	21	-1.0337626292791668
2215	22	-0.8229314686202684
2215	23	-0.8438320880996457
2215	24	-0.8874657253312108
2215	25	-0.8234697856416631
2215	26	-0.8816276806862504
2215	27	-0.8541358862658939
2215	28	-0.9306670640879346
2215	29	-1.0333961326222862
2215	30	-0.9463606349850835
2215	31	-0.8938555669523254
2215	32	-1.0218831954986527
2215	33	-0.8792700464015368
2215	34	-0.9512776847749649
2215	35	-1.0031994004931732
2215	36	-1.0301436945389568
2215	37	-0.9881937178282669
2215	38	-1.0292649206531976
2215	39	-0.9181539830086862
2215	40	-1.0239189554715153
2215	41	-1.0438917817766438
2215	42	-0.9777689156504579
2215	43	-1.003035076745192
2215	44	-0.8917235003999977
2215	45	-0.8138660791015653
2215	46	-0.8361097017497485
2215	47	-0.8380241296271862
2215	48	-0.9117783044450007
2215	49	-0.7412250077581035
2215	50	-0.8202858215080369
2216	1	-0.4149200904326449
2216	2	-0.43565295136761506
2216	3	-0.4071272120436191
2216	4	-0.39092612688045136
2216	5	-0.6011287595806891
2216	6	-0.5650494794600291
2216	7	-0.5623063278594955
2216	8	-0.5337553038115885
2216	9	-0.7775987382073453
2216	10	-0.7003532005589506
2216	11	-0.7216188850063645
2216	12	-0.7012014972062701
2216	13	-0.8881247287126257
2216	14	-1.0125437082149986
2216	15	-0.9902280461167408
2216	16	-0.9437594195929222
2216	17	-0.8953950059604097
2216	18	-1.054692760314957
2216	19	-1.0885560597262227
2216	20	-1.0962110678685875
2216	21	-1.010762841741851
2216	22	-1.1067889439878016
2216	23	-1.0503144364475645
2216	24	-1.0723817074024993
2216	25	-1.0638785229257195
2216	26	-1.1408287781642699
2216	27	-1.1244439761930423
2216	28	-1.1499775191792025
2216	29	-1.2250988818608803
2216	30	-1.21118825827471
2216	31	-1.1524871549907516
2216	32	-1.2639966067952457
2216	33	-1.2066677163544384
2216	34	-1.167914774002874
2216	35	-1.2844458688660936
2216	36	-1.2982351301038761
2216	37	-1.4049986359230715
2216	38	-1.3113516753772814
2216	39	-1.295598178245001
2216	40	-1.5067508760747117
2216	41	-1.53292128411195
2216	42	-1.3674360915455352
2216	43	-1.2926295680374338
2216	44	-1.141462989742486
2216	45	-1.176979844661247
2216	46	-1.2370503428160475
2216	47	-1.385383085214869
2216	48	-1.2858347785969997
2216	49	-1.1745657800611582
2216	50	-1.2805313646095335
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	108706.0
3	17184.0
4	28932.0
5	17764.0
6	13172.0
7	10927.0
8	8929.0
9	7840.0
10	7483.0
11	7720.0
12	7714.0
13	7929.0
14	8603.0
15	9330.0
16	9883.0
17	10868.0
18	11800.0
19	12563.0
20	14912.0
21	18268.0
22	21829.0
23	26356.0
24	30693.0
25	35584.0
26	39240.0
27	45189.0
28	52534.0
29	61452.0
30	72210.0
31	86363.0
32	105645.0
33	130256.0
34	165771.0
35	213930.0
36	280588.0
37	379342.0
38	589954.0
39	1342817.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.730114892093866	17.318088347192074	13.85727152855562	27.094525232158446
2	15.608570773259196	18.949653896346522	37.69246154251829	27.749313787876
3	19.00495438375157	22.797030454112875	28.009580431999975	30.188434730135576
4	13.028446057464246	15.30432965636128	34.75911459143074	36.90810969474374
5	14.887060691347335	36.40037526160064	33.371357187827385	15.341206859224643
6	34.64632991194356	34.532872437223304	16.506718996438778	14.314078654394349
7	30.247493356296335	30.26020726912368	20.291355111527885	19.2009442630521
8	27.52064526130012	34.041683907855784	18.8928432000211	19.544827630822994
9	26.64491977412095	15.547858714868132	18.174679301760527	39.632542209250396
10	15.191202305177192	28.48672980357223	32.543408696171475	23.778659195079104
11	36.652079019642436	21.929396518324225	21.996632241744027	19.421892220289312
12	24.3051752404787	23.732365786895773	29.061427039761252	22.901031932864274
13	29.04272564967466	20.296338548051608	25.061133699762234	25.5998021025115
14	22.65803865434463	19.878538238256972	26.020858136006915	31.442564971391484
15	24.573730299117948	27.695660007545392	22.736209009274457	24.994400684062207
16	24.529405850309796	26.83195490817578	24.260722448752052	24.37791679276237
17	23.583852403284748	26.805885591241996	25.6311714737671	23.979090531706156
18	23.57495552160484	26.39855280031596	26.42005473536568	23.60643694271352
19	24.53154318990618	26.006196650324764	26.36311375457283	23.099146405196226
20	24.69900865720284	25.322392959564883	26.147922997978295	23.83067538525399
21	24.850491179278198	25.193455654509204	26.039096257999265	23.91695690821333
22	24.392608577935626	24.923130444823137	26.245018036598765	24.43924294064247
23	24.279137176889886	25.075333154327144	26.2528172944865	24.39271237429647
24	24.027974777411153	25.20594134304811	26.238360432188163	24.527723447352574
25	24.22049090602792	25.046190873504564	26.287826437279577	24.44549178318794
26	24.111845526840845	25.29981730019355	26.44740201472448	24.140935158241124
27	23.990185722266393	25.45941594596175	26.253143169260206	24.29725516251165
28	23.83977767885115	25.33322417672819	26.377096422690872	24.449901721729788
29	24.123176885497223	25.542452225709493	26.077147046798988	24.257223841994296
30	24.132400116652086	25.546931064788687	26.318905102732465	24.001763715826762
31	24.14389160132194	25.048930788252232	26.391622471784864	24.415555138640965
32	23.986574325761694	25.419682944094106	26.083079445606238	24.510663284537955
33	24.116871691627598	25.298655403811765	25.983069890937532	24.601403013623106
34	24.28886007881223	25.389141288049267	26.082523703005428	24.239474930133078
35	24.478119169050878	25.283119791018784	26.006866525687272	24.231894514243066
36	24.2995720619469	25.51367617969069	25.897977040467897	24.28877471789451
37	24.22501747725469	25.115747939425454	26.26551495082734	24.393719632492516
38	24.372276398149822	25.28032404222878	25.988109130902803	24.359290428718598
39	24.5405140232717	25.36965603915401	25.87564909655114	24.21418084102315
40	24.348570645323466	25.03575345699672	26.36173468385088	24.253941213828934
41	24.08939371852934	25.48918983864235	25.97194935142017	24.44946709140814
42	24.295408961183593	25.345401908992155	26.010148688056073	24.349040441768178
43	24.097691346548263	25.02860039765685	26.29268946721794	24.581018788576955
44	24.32665923229638	25.090974851911675	25.96573639195566	24.616629523836295
45	24.220686134992768	25.287008935689553	25.81678739536204	24.675517533955645
46	24.219792793801226	25.253684564008804	25.904157805057025	24.62236483713294
47	24.28931679292854	25.22767433391639	25.7182698641068	24.76473900904827
48	24.059240686431803	25.54712316023292	25.945112320003183	24.44852383333209
49	24.550267419317766	25.132615207207067	25.774088259568117	24.543029113907046
50	24.37521018687479	25.185287814235597	25.87574547936537	24.563756519524247
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	930.0
1	638.0
2	346.0
3	477.0
4	608.0
5	690.0
6	772.0
7	718.0
8	664.0
9	820.0
10	976.0
11	1508.0
12	2040.0
13	3469.5
14	4899.0
15	6712.5
16	8526.0
17	9667.5
18	10809.0
19	11227.0
20	11645.0
21	12313.5
22	12982.0
23	14968.0
24	16954.0
25	21217.0
26	25480.0
27	32165.5
28	38851.0
29	47588.5
30	56326.0
31	66841.5
32	77357.0
33	90790.5
34	104224.0
35	121248.0
36	138272.0
37	148916.5
38	159561.0
39	174647.0
40	189733.0
41	205322.5
42	220912.0
43	230637.5
44	240363.0
45	255639.5
46	270916.0
47	288700.5
48	306485.0
49	310569.5
50	314654.0
51	313537.5
52	312421.0
53	303706.5
54	294992.0
55	287363.5
56	279735.0
57	263721.0
58	247707.0
59	225187.0
60	202667.0
61	178382.0
62	154097.0
63	133179.0
64	112261.0
65	94492.0
66	76723.0
67	64340.0
68	51957.0
69	41226.5
70	30496.0
71	24401.0
72	18306.0
73	14850.0
74	11394.0
75	8542.0
76	5690.0
77	4395.0
78	3100.0
79	2366.5
80	1633.0
81	1264.0
82	895.0
83	668.0
84	441.0
85	356.5
86	272.0
87	197.5
88	123.0
89	84.0
90	45.0
91	34.0
92	23.0
93	14.5
94	6.0
95	5.0
96	4.0
97	4.0
98	4.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0018157939248012577
2	0.009601321301003909
3	4.974777876167829E-5
4	0.005347886216880416
5	0.0435293064164685
6	0.028679594456107536
7	0.02639119663307033
8	0.03280866009332683
9	0.03270916453580348
10	0.03731083407125872
11	0.03937536688986837
12	0.03275891231456515
13	0.05069298655815018
14	0.04716089426607102
15	0.04805635428378123
16	0.05392659217765927
17	0.05462306108032276
18	0.05066811266876933
19	0.0492503009740615
20	0.04706139870854767
21	0.044474514212940394
22	0.044375018655417035
23	0.044698379217367946
24	0.04129065637219298
25	0.04051956580138697
26	0.041439899708478015
27	0.04091754803148039
28	0.0395246102261534
29	0.03828091575711144
30	0.037111842956212004
31	0.033281263991562775
32	0.027734386659635644
33	0.023182464902942082
34	0.02121742764185579
35	0.019401633717054535
36	0.019227516491388662
37	0.01915289482324614
38	0.013854756385127403
39	0.011491736893947686
40	0.009675942969146428
41	0.006616454575303212
42	0.0062930940133523044
43	0.005745868446973843
44	0.005298138438118738
45	0.0027112539425114667
46	0.0032336056195090886
47	0.0015919289203737052
48	0.00174117225665874
49	4.974777876167829E-5
50	0.0011939466902802789
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	4020280.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.856187223119164
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.86815414843588	39.57245172719072
2	13.069479348294019	13.816056946842362
3	4.779139770654588	7.578213194495211
4	2.417386541384381	5.110953424882454
5	1.3047318085943413	3.4481574375510684
6	0.7945791275601577	2.519905387794143
7	0.5271838041858777	1.9505448098531462
8	0.36944310202739883	1.5621883015240086
9	0.2844157645299871	1.3529819609283216
>10	1.4427650648042731	13.614906640701676
>50	0.09338085072505278	3.4134229605124133
>100	0.04583689799646364	4.4013869465197715
>500	0.002604154027778252	0.9252540647762726
>1k	8.996167799634661E-4	0.7335761964284685
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4873889380839145E-5
2	0.0	0.0	0.0	0.0	2.4873889380839145E-5
3	0.0	0.0	0.0	0.0	2.4873889380839145E-5
4	0.0	0.0	0.0	0.0	2.4873889380839145E-5
5	0.0	0.0	0.0	0.0	2.4873889380839145E-5
6	0.0	0.0	0.0	0.0	2.4873889380839145E-5
7	0.0	0.0	0.0	0.0	2.4873889380839145E-5
8	0.0	0.0	0.0	0.0	2.4873889380839145E-5
9	0.0	0.0	0.0	0.0	2.4873889380839145E-5
10	0.0	0.0	0.0	0.0	2.4873889380839145E-5
11	0.0	0.0	0.0	2.4873889380839145E-5	4.974777876167829E-5
12	0.0	0.0	0.0	4.974777876167829E-5	1.2436944690419574E-4
13	0.0	0.0	0.0	4.974777876167829E-5	1.74117225665874E-4
14	0.0	0.0	0.0	4.974777876167829E-5	1.74117225665874E-4
15	0.0	0.0	0.0	7.462166814251743E-5	2.487388938083915E-4
16	0.0	0.0	0.0	7.462166814251743E-5	2.984866725700697E-4
17	0.0	0.0	0.0	7.462166814251743E-5	2.984866725700697E-4
18	0.0	0.0	0.0	9.949555752335658E-5	2.984866725700697E-4
19	0.0	0.0	0.0	9.949555752335658E-5	2.984866725700697E-4
20	0.0	0.0	0.0	1.2436944690419574E-4	3.731083407125872E-4
21	0.0	0.0	0.0	1.4924333628503486E-4	3.979822300934263E-4
22	0.0	0.0	0.0	1.74117225665874E-4	4.228561194742655E-4
23	0.0	0.0	0.0	2.238650044275523E-4	4.228561194742655E-4
24	0.0	0.0	0.0	2.984866725700697E-4	4.228561194742655E-4
25	0.0	0.0	0.0	3.731083407125872E-4	4.228561194742655E-4
26	0.0	0.0	0.0	5.472255663784612E-4	4.477300088551046E-4
27	0.0	0.0	0.0	7.959644601868526E-4	4.97477787616783E-4
28	0.0	0.0	0.0	0.0015421811416120271	4.97477787616783E-4
29	0.0	0.0	0.0	0.0025122628274647537	4.97477787616783E-4
30	0.0	0.0	0.0	0.004726038982359438	4.97477787616783E-4
31	0.0	0.0	0.0	0.011193250221377616	4.97477787616783E-4
32	0.0	0.0	0.0	0.019103147044484462	4.97477787616783E-4
33	0.0	0.0	0.0	0.027734386659635648	4.97477787616783E-4
34	0.0	0.0	0.0	0.039549484115534245	4.97477787616783E-4
35	0.0	0.0	0.0	0.054846926084750315	4.97477787616783E-4
36	0.0	0.0	0.0	0.07263175699205031	4.97477787616783E-4
37	0.0	0.0	0.0	0.0971574119215577	4.97477787616783E-4
38	0.0	0.0	0.0	0.12287701354134538	5.22351676997622E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	7455	0.0	19.506437	1
GGTATCA	4165	0.0	16.955639	1
CGCGTAA	80	0.0019867439	16.50334	10
GACCGTA	160	3.8232247E-7	15.127309	7
TATCAAC	9810	0.0	14.734911	2
CGCGATC	135	1.9563095E-5	14.668177	23
CAACGCA	9890	0.0	14.482966	5
ATCAACG	9970	0.0	14.432421	3
TCAACGC	9990	0.0	14.4255495	4
GTACATG	11690	0.0	14.189955	1
ACCGTCG	235	8.221832E-10	14.044874	8
GGACCGT	345	0.0	14.030081	6
CTAGTAC	300	0.0	13.93442	3
CGTCGTA	190	1.7516868E-7	13.897551	10
AACGCAG	10310	0.0	13.871111	6
GCGATAC	175	1.0518052E-6	13.831544	14
TAGATCG	145	3.835986E-5	13.656749	5
TACATGG	11900	0.0	13.552149	2
TATACTG	785	0.0	13.453781	5
TACCGAT	365	0.0	13.2623	7
>>END_MODULE