FastQCFastQC Report
Mon 6 Feb 2017
SRR2077127_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077127_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671650
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC41770.1563453296651882No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG27550.10311979488331181No Hit
ATATCATACTTGGCATCTTCATAAATGTTCCTGATGTGAGGGGGCACATC27270.10207175341081355No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACG551.593241E-424.002424
GGTATCA22900.022.1964361
GTATCAA40950.019.6668131
TTATAGG13950.018.1390344
CTTATAG15250.017.7473683
TATAGGC14050.017.3815465
TAGGCCT14750.017.1513677
ATAGGCC14550.016.9335486
TCTTATA16450.016.8540122
ACCGTAT1206.417691E-616.498568
CGGTTCG953.3475686E-416.21093430
TATCATA9400.016.1517622
GTATTAG2850.015.44158651
TCAACGC52550.015.32494454
ATCAACG53050.015.180793
GGTGATC17150.015.13853919
TCATACT10650.015.0821684
CAACGCA53400.015.0381125
CTCTTAT17750.014.87612151
AACGCAG54850.014.679036
Produced by FastQC (version 0.11.5)