FastQCFastQC Report
Mon 6 Feb 2017
SRR2077127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671650
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC44960.16828551644115058No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30840.11543428218516646No Hit
ATATCATACTTGGCATCTTCATAAATGTTCCTGATGTGAGGGGGCACATC29240.10944547377089064No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC28810.10783598150955402No Hit
CTCTAGAGTAGCTCTAGCTCCTAAAACATCCATACCTTTGTCCATTTGCT27040.1012108622012614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27250.025.7566811
GTATCAA55100.022.4014951
CGCACGA654.933505E-420.30818628
TATCATA9400.018.9600372
TCATACT10800.016.7066124
TATAGGC13750.016.4828685
CTTATAG15150.016.2665543
CATACTT11950.016.2036575
ATAGGCC13900.016.146096
TTATAGG14800.016.0568474
TATCAAC77050.015.79190352
ATCAACG77250.015.7513143
TCTTATA16450.015.6495532
ATACTTG12700.015.5926816
TCAACGC78500.015.5568474
ATTATCG850.002987422315.52455640
CAACGCA79650.015.2217325
TAGGCCT14950.015.159837
ATCATAC14700.014.9683113
CCTATAC2509.094947E-1214.9623253
Produced by FastQC (version 0.11.5)