FastQCFastQC Report
Mon 6 Feb 2017
SRR2077126_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077126_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938310
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37750.023.2009961
GGTATCA18900.021.307371
TATCAAC49250.017.7382182
ATCAACG50650.016.9878273
TCAACGC50500.016.9072694
CAACGCA50300.016.842395
AACGCAG52050.016.4413036
TGCGATA1958.949428E-1015.79297113
CTAGTAC1603.8170583E-715.1291053
GTCTTAT3900.014.6706481
GTACATG91900.014.3433921
CGCACGA3450.014.02610335
GTGTAGG8050.013.9416711
TACATGG94050.013.8278432
GCGATAC2256.7357178E-913.68817414
TAGATTG1658.706367E-613.3360435
ACGCAGA63900.012.9791997
GTATAAT3400.012.9446891
ACATGGG97950.012.8957913
TATACTG3650.012.6601075
Produced by FastQC (version 0.11.5)