##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077126_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2938310 Sequences flagged as poor quality 0 Sequence length 50 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.42626850128135 31.0 30.0 34.0 25.0 34.0 2 29.548519386994563 31.0 30.0 34.0 25.0 34.0 3 29.655360394240226 31.0 30.0 34.0 25.0 34.0 4 33.079187696328844 37.0 35.0 37.0 26.0 37.0 5 33.03560482045802 37.0 35.0 37.0 26.0 37.0 6 33.10569238780115 37.0 35.0 37.0 26.0 37.0 7 32.95734316665022 37.0 35.0 37.0 26.0 37.0 8 32.84990113364485 37.0 35.0 37.0 25.0 37.0 9 34.27717735705218 39.0 35.0 39.0 25.0 39.0 10 34.223741538503425 39.0 35.0 39.0 25.0 39.0 11 34.24763860858793 38.0 35.0 39.0 25.0 39.0 12 34.15891039407006 38.0 35.0 39.0 25.0 39.0 13 34.101677494886516 38.0 35.0 39.0 25.0 39.0 14 35.07843624396337 39.0 35.0 41.0 24.0 41.0 15 35.12025994534273 39.0 35.0 41.0 25.0 41.0 16 35.083440140761184 39.0 35.0 41.0 25.0 41.0 17 34.920214000564954 39.0 35.0 41.0 23.0 41.0 18 34.89411498446386 39.0 34.0 41.0 23.0 41.0 19 35.01093962175536 39.0 35.0 41.0 24.0 41.0 20 34.98967467694015 39.0 35.0 41.0 23.0 41.0 21 34.93568309674609 39.0 35.0 41.0 23.0 41.0 22 34.85787578574078 39.0 34.0 41.0 23.0 41.0 23 34.80891294655771 39.0 34.0 41.0 23.0 41.0 24 34.813040489260835 39.0 34.0 41.0 23.0 41.0 25 34.714058081005746 39.0 34.0 41.0 22.0 41.0 26 34.65651888330367 39.0 34.0 41.0 21.0 41.0 27 34.595332350909196 39.0 34.0 41.0 21.0 41.0 28 34.48311035935623 39.0 34.0 41.0 19.0 41.0 29 34.46661652446474 39.0 34.0 41.0 19.0 41.0 30 34.3054293114069 39.0 34.0 41.0 18.0 41.0 31 34.29856686326494 39.0 34.0 41.0 18.0 41.0 32 34.17024718290446 39.0 34.0 41.0 17.0 41.0 33 34.11744778461088 39.0 34.0 41.0 16.0 41.0 34 34.03924024354136 39.0 33.0 41.0 16.0 41.0 35 33.97271118432024 39.0 33.0 41.0 16.0 41.0 36 33.883137926222894 38.0 33.0 41.0 15.0 41.0 37 33.78588372227573 38.0 33.0 40.0 15.0 41.0 38 33.69694314078501 38.0 33.0 40.0 14.0 41.0 39 33.58052451919641 38.0 33.0 40.0 9.0 41.0 40 33.51492558647658 38.0 33.0 40.0 9.0 41.0 41 33.39570365278 38.0 33.0 40.0 9.0 41.0 42 33.24991236459053 38.0 33.0 40.0 8.0 41.0 43 33.170986042997505 38.0 33.0 40.0 7.0 41.0 44 33.01890576555911 38.0 33.0 40.0 2.0 41.0 45 32.875139110577166 38.0 32.0 40.0 2.0 41.0 46 32.73630045842678 38.0 32.0 40.0 2.0 41.0 47 32.56811908886401 38.0 32.0 40.0 2.0 41.0 48 32.39786884297436 38.0 32.0 40.0 2.0 41.0 49 32.218854715806025 38.0 32.0 40.0 2.0 41.0 50 31.99791036344021 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.6376402794776084 1101 2 1.999877265949646 1101 3 2.0953623199292863 1101 4 2.353767851974524 1101 5 2.3686363999327753 1101 6 2.213070886101818 1101 7 2.3264506333260826 1101 8 2.282725496184426 1101 9 2.651271439637334 1101 10 2.7215762585497245 1101 11 2.604092408841545 1101 12 2.777368357344365 1101 13 2.7420193268754645 1101 14 3.2010402959122572 1101 15 3.1694186179312425 1101 16 3.2091359046842243 1101 17 3.3048157654654275 1101 18 3.3088336942983716 1101 19 3.2111437630431467 1101 20 3.3121025384224936 1101 21 3.3074830701405986 1101 22 3.3197979373223347 1101 23 3.3704296851799285 1101 24 3.4075400025983384 1101 25 3.282310986507369 1101 26 3.309140468572757 1101 27 3.3728089704108086 1101 28 3.417733861782594 1101 29 3.3779744776333374 1101 30 3.4218863700952866 1101 31 3.4051447658097374 1101 32 3.372138191372464 1101 33 3.485954822369145 1101 34 3.5838612479987546 1101 35 3.585070866967385 1101 36 3.481415240084843 1101 37 3.6812431354975104 1101 38 3.6857233376604626 1101 39 3.7119307783346613 1101 40 3.6119430712488523 1101 41 3.642752553996665 1101 42 3.666636297403258 1101 43 3.7711891853533643 1101 44 3.74029939600036 1101 45 3.663003692812069 1101 46 3.6682033887041783 1101 47 3.710824658289681 1101 48 3.6154981303672855 1101 49 3.766952879274122 1101 50 3.939239029919108 1102 1 1.0609098084437747 1102 2 1.1857596872473053 1102 3 1.2032232939536094 1102 4 1.4114405359340978 1102 5 1.4457191204609359 1102 6 1.3877049279287164 1102 7 1.5756435462115164 1102 8 1.482047869837615 1102 9 1.6192603129110381 1102 10 1.8219601015553906 1102 11 1.7241804349007452 1102 12 1.7641590013072772 1102 13 1.7831217588495534 1102 14 1.9646536494363858 1102 15 1.874854517601868 1102 16 1.903857246282712 1102 17 2.088438342842295 1102 18 2.145709495484404 1102 19 2.0327330256070155 1102 20 1.9791686632785996 1102 21 2.0337870287712576 1102 22 1.8941098705910306 1102 23 1.9523803484620998 1102 24 1.8108384378116682 1102 25 1.928547182503344 1102 26 1.9809310943784837 1102 27 1.8611702113458293 1102 28 1.9861755753855945 1102 29 1.9966353485248192 1102 30 2.042557241967252 1102 31 2.073423790986496 1102 32 2.226955112421372 1102 33 2.1171677590041824 1102 34 2.081660596518759 1102 35 2.190358967107919 1102 36 2.2125925886266415 1102 37 2.068358066881494 1102 38 2.15424058517565 1102 39 2.2465801448302827 1102 40 2.1708885742575035 1102 41 2.153209279964713 1102 42 2.081878532480772 1102 43 2.002359271018989 1102 44 1.8610773952958581 1102 45 1.9659673094338928 1102 46 1.9465004621282205 1102 47 1.7320522296202014 1102 48 1.7931626072767912 1102 49 1.8162841499506257 1102 50 1.807455957504601 1103 1 0.2857936222339603 1103 2 0.42802206877011173 1103 3 0.34443599142122494 1103 4 0.4278130959730646 1103 5 0.5029798767775517 1103 6 0.44352240664155573 1103 7 0.4653357561240199 1103 8 0.5855340130562539 1103 9 0.6764187976855709 1103 10 0.6402063572524739 1103 11 0.588227296193871 1103 12 0.5039602839881852 1103 13 0.5616056490564958 1103 14 0.648458393823347 1103 15 0.6012204249276536 1103 16 0.5018170134006184 1103 17 0.6900510266737072 1103 18 0.5796569306120105 1103 19 0.6385067773288355 1103 20 0.643031807078799 1103 21 0.5066729190450303 1103 22 0.5428263627378911 1103 23 0.5529212062806863 1103 24 0.3825128997300311 1103 25 0.49117449454057294 1103 26 0.559844727580078 1103 27 0.44820481254251376 1103 28 0.4984731376438205 1103 29 0.6465858497735155 1103 30 0.6882575674472804 1103 31 0.5697730682169677 1103 32 0.6586912207900468 1103 33 0.6692172233274434 1103 34 0.6545899943891484 1103 35 0.552079910260808 1103 36 0.6331549403455625 1103 37 0.603921392374005 1103 38 0.6118736706608203 1103 39 0.6758776000915461 1103 40 0.6730072490748356 1103 41 0.6645779600981498 1103 42 0.6794251630722457 1103 43 0.5747467647282818 1103 44 0.6125465783150048 1103 45 0.5268379474091702 1103 46 0.5033847039062493 1103 47 0.5840985549159399 1103 48 0.4668424689975623 1103 49 0.5713888668964131 1103 50 0.6621625242489273 1104 1 0.33835187119758814 1104 2 0.37250389061279776 1104 3 0.2722668876541512 1104 4 0.28124917190402243 1104 5 0.35949710214651986 1104 6 0.27225845874008314 1104 7 0.3091997339674961 1104 8 0.37676144133570233 1104 9 0.41475775474100374 1104 10 0.2325855952262188 1104 11 0.355727376321326 1104 12 0.35818200215125273 1104 13 0.33308472242323006 1104 14 0.40599561285519314 1104 15 0.41635099615064775 1104 16 0.45252218361284946 1104 17 0.439771551804796 1104 18 0.3579833984210481 1104 19 0.4360395545475342 1104 20 0.4216210189580565 1104 21 0.4583251888998845 1104 22 0.4180732952205233 1104 23 0.36771091783154475 1104 24 0.4069017561439807 1104 25 0.44452236782827725 1104 26 0.3878147935334155 1104 27 0.36522805218845633 1104 28 0.36133248039699595 1104 29 0.37070637978856524 1104 30 0.3991976043109986 1104 31 0.40138519895482716 1104 32 0.3924681156934824 1104 33 0.4749230939901139 1104 34 0.438663994515224 1104 35 0.3815263102161097 1104 36 0.4444744044364555 1104 37 0.4358252656914985 1104 38 0.5457310768963097 1104 39 0.5927477072579208 1104 40 0.49109372577490973 1104 41 0.46419622501511526 1104 42 0.5984363962253028 1104 43 0.6000059464217955 1104 44 0.5810322747655476 1104 45 0.6648381537749586 1104 46 0.6871250408004812 1104 47 0.6264865595044569 1104 48 0.5275375517059828 1104 49 0.5834411006580638 1104 50 0.6383231595262586 1105 1 0.2984010261162666 1105 2 0.3053937143181997 1105 3 0.23229266537556015 1105 4 0.357268720823555 1105 5 0.28689633748537346 1105 6 0.2761108551165137 1105 7 0.39421514241903566 1105 8 0.3997781238444915 1105 9 0.3701696326077055 1105 10 0.31230362610208573 1105 11 0.20924681773192333 1105 12 0.32447627064691176 1105 13 0.32111838872751974 1105 14 0.48234392541844073 1105 15 0.46768692883060226 1105 16 0.36958542669479755 1105 17 0.4424635930815981 1105 18 0.3413674448621222 1105 19 0.2511482109753942 1105 20 0.4080766372181941 1105 21 0.4650830743207379 1105 22 0.4030180312644944 1105 23 0.34666149625580545 1105 24 0.4058134080456526 1105 25 0.35578579804991506 1105 26 0.3672044358474693 1105 27 0.4177072868260012 1105 28 0.4803652872403745 1105 29 0.39537529805150484 1105 30 0.38444585139192355 1105 31 0.18780253269301994 1105 32 0.4024208702033434 1105 33 0.4265471680721049 1105 34 0.43257619726391283 1105 35 0.38534499283603196 1105 36 0.4656343879433251 1105 37 0.42054303357389955 1105 38 0.4546348058471281 1105 39 0.5393657028324768 1105 40 0.4728160285718914 1105 41 0.46891514780084975 1105 42 0.5679054276757469 1105 43 0.6084980622439389 1105 44 0.47177344793274756 1105 45 0.5140693187540322 1105 46 0.4698541855273959 1105 47 0.3147823382717334 1105 48 0.4456806817364978 1105 49 0.5337505999211203 1105 50 0.48241042520801614 1106 1 0.16126415418291273 1106 2 0.036648966911979386 1106 3 0.006077545705348797 1106 4 0.03508056471807208 1106 5 0.044763731911722004 1106 6 0.05909251555542028 1106 7 0.15267408443897068 1106 8 0.20619844877353444 1106 9 0.24220674115152718 1106 10 0.2114409257428207 1106 11 0.16932303364443868 1106 12 0.16288447920011606 1106 13 0.2177756284035084 1106 14 0.2618367278917688 1106 15 0.36336999851819485 1106 16 0.21546672889662943 1106 17 0.36193717872228603 1106 18 0.08814033267121602 1106 19 0.30067659716002026 1106 20 0.20565224778333402 1106 21 0.3292429100262524 1106 22 0.3108684331080056 1106 23 0.30826631606371535 1106 24 0.1786902458909836 1106 25 0.3094837312118486 1106 26 0.26990756363983337 1106 27 0.2842221702285954 1106 28 0.20262029940946746 1106 29 0.0760421530008486 1106 30 0.2796991565885705 1106 31 0.1672011366869839 1106 32 0.3307919693556016 1106 33 0.18516142187058193 1106 34 0.30361843615348505 1106 35 0.3111892756354635 1106 36 0.33192264643286506 1106 37 0.2829643973433136 1106 38 0.313634639135536 1106 39 0.4268043484657724 1106 40 0.46657394362269144 1106 41 0.5198045691573299 1106 42 0.4561109466437969 1106 43 0.3769864852165412 1106 44 0.4902531496175584 1106 45 0.4173337803420125 1106 46 0.4214507811823509 1106 47 0.27590150877654906 1106 48 0.30095508944963 1106 49 0.36499578124890775 1106 50 0.46612728806437786 1107 1 -0.12612528158608782 1107 2 -0.2772550600830712 1107 3 -0.2201154509612735 1107 4 -0.20889699751358393 1107 5 -0.320595639274309 1107 6 -0.20960978599250524 1107 7 -0.1358137105322612 1107 8 -0.1734089783003938 1107 9 -0.10653774123687754 1107 10 -0.2981030261219004 1107 11 -0.2509916094918694 1107 12 -0.4823959918307139 1107 13 -0.22825627472138876 1107 14 -0.39306560332529017 1107 15 -0.26277344940055514 1107 16 -0.25898756399357836 1107 17 -0.18212739437381487 1107 18 -0.14662236467846412 1107 19 -0.2731344851430677 1107 20 -0.1884104039033545 1107 21 -0.22709313610818782 1107 22 -0.535835340733307 1107 23 -0.4850510434517403 1107 24 -0.4878672045726091 1107 25 -0.4507596673513632 1107 26 -0.2517637233545429 1107 27 -0.3370362176895796 1107 28 -0.32928864338813923 1107 29 -0.18818796615444455 1107 30 -0.27977456185818284 1107 31 -0.3414464293810582 1107 32 -0.25684304185834606 1107 33 -0.2742832070816448 1107 34 -0.2523914641500511 1107 35 -0.26515471804943047 1107 36 -0.3366292055374629 1107 37 -0.35271984660570155 1107 38 -0.36709328091837534 1107 39 -0.20233366433318167 1107 40 -0.1260669939048782 1107 41 -0.08953416759433708 1107 42 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-2.400590288233033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 127483.0 3 6331.0 4 12099.0 5 9700.0 6 8121.0 7 7074.0 8 6389.0 9 6004.0 10 5964.0 11 6047.0 12 6198.0 13 6475.0 14 6975.0 15 7430.0 16 7977.0 17 8445.0 18 9105.0 19 10393.0 20 12402.0 21 15591.0 22 18834.0 23 21588.0 24 23486.0 25 26180.0 26 29623.0 27 33780.0 28 38685.0 29 45208.0 30 52989.0 31 64190.0 32 78602.0 33 98279.0 34 126871.0 35 165837.0 36 221284.0 37 304474.0 38 477590.0 39 824607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.04062091157088 15.670922181004176 12.379163023519038 25.9092938839059 2 17.144006562103577 18.03181857200669 37.53360421086481 27.290570655024926 3 20.800369285905305 21.645093760805615 26.645545148097614 30.908991805191466 4 14.303293097386616 14.398068783573487 33.323499191919744 37.975138927120156 5 16.351534747969406 35.32853294526904 32.050595517911866 16.269336788849692 6 36.475118721451864 32.440700567234174 15.884702111821886 15.199478599492084 7 32.156069842439436 29.431031118832184 18.79282453004744 19.620074508680933 8 28.588493488406147 33.076649351224255 18.189795636917136 20.145061523452465 9 27.57618241212721 15.447576672774632 17.329308728486623 39.64693218661154 10 16.590082823587732 27.38547662547164 30.84482374707448 25.179616803866146 11 37.61444586918935 21.332301433650507 21.04588457931929 20.007368117840855 12 25.41469123707777 22.815075273189013 27.48051130808938 24.289722181643835 13 29.87447006359407 19.72550051772118 24.06972773075366 26.330301687931083 14 24.042425105027636 19.235056928419127 24.31203406647812 32.410483900075114 15 25.80240406256746 26.604688950314998 21.54006555296095 26.052841434156594 16 26.143146331787992 25.67297231496347 22.879713700526256 25.30416765272228 17 25.066228548079543 25.79504903296105 24.106340234268593 25.03238218469082 18 25.142410391863955 25.422208632032795 24.538445272492567 24.89693570361068 19 25.97288229789425 25.027617611522707 24.49556143096687 24.50393865961617 20 26.160518465038347 24.518485471808635 24.399801114304786 24.921194948848232 21 26.290728985229833 24.317127682256526 24.36879566928946 25.023347663224175 22 26.352865201566512 23.896934352513696 24.201400975628363 25.548799470291428 23 25.79258139321068 24.05895112491359 24.481640769987774 25.666826711887953 24 25.66043005222817 24.258358295117254 24.263569191680958 25.817642460973623 25 25.826256133440783 24.050002145810186 24.438734394339967 25.685007326409064 26 25.605292135945113 24.577832470872575 24.453649021331554 25.36322637185075 27 25.646436556902184 24.509772200528925 24.342886141134517 25.50090510143437 28 25.725728624146544 24.4515965740179 24.226461191313298 25.596213610522263 29 25.645301656235365 24.601456124664708 24.300319325584365 25.452922893515563 30 25.72027919152575 24.595536382396887 24.288899810595804 25.395284615481557 31 25.862870347007345 24.197724074219735 24.107521982716204 25.831883596056716 32 25.683083367411598 24.62375722155617 24.174605114906463 25.518554296125767 33 25.780167551254387 24.322683435185493 24.16993073690067 25.727218276659446 34 26.017438049705966 24.243669624309348 24.174821777030356 25.564070548954327 35 25.689710066213667 24.431360998125452 24.345959161713555 25.532969773947332 36 25.875474125411213 24.445683164534074 24.043466135553132 25.635376574501578 37 25.951760466138644 24.157633374502876 24.262787671748328 25.62781848761015 38 25.95927878823785 24.28267322586115 24.211272525950424 25.546775459950577 39 26.05921988133959 24.46894289808171 24.00339963715411 25.468437583424592 40 25.968898804659325 24.24143675155002 24.27524980113977 25.51441464265089 41 25.662758978535404 24.427295346864067 24.307620128043972 25.602325546556553 42 26.05264600351146 24.310330681071115 24.23423886565658 25.402784449760844 43 25.73706250851151 24.111228380770804 24.308082527577284 25.843626583140406 44 25.76851765478106 24.350095564669058 24.137790158098756 25.743596622451125 45 25.708493617021276 24.422059574468086 24.07251063829787 25.796936170212764 46 25.919639595845712 24.06352688308209 24.096104597270347 25.92072892380185 47 25.704089299313317 24.380422863799755 24.229237157300197 25.68625067958673 48 25.731042925070398 24.43031416293117 24.181368090240145 25.657274821758286 49 25.80401490876399 24.258944628627237 24.134693581599407 25.80234688100937 50 25.929132142784194 24.28989167954575 24.12666208239435 25.654314095275705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1321.0 1 974.0 2 627.0 3 605.5 4 584.0 5 598.0 6 612.0 7 635.5 8 659.0 9 768.0 10 877.0 11 983.5 12 1090.0 13 1467.5 14 1845.0 15 2432.0 16 3019.0 17 3180.0 18 3341.0 19 3269.5 20 3198.0 21 3260.0 22 3322.0 23 3638.5 24 3955.0 25 4743.0 26 5531.0 27 6826.0 28 8121.0 29 10389.5 30 12658.0 31 16635.5 32 20613.0 33 27372.5 34 34132.0 35 44067.5 36 54003.0 37 66549.0 38 79095.0 39 94736.5 40 110378.0 41 126430.0 42 142482.0 43 157233.0 44 171984.0 45 188215.0 46 204446.0 47 218283.5 48 232121.0 49 239239.0 50 246357.0 51 252506.0 52 258655.0 53 257736.5 54 256818.0 55 249174.0 56 241530.0 57 229163.5 58 216797.0 59 199945.5 60 183094.0 61 162384.0 62 141674.0 63 122782.0 64 103890.0 65 87597.5 66 71305.0 67 58876.5 68 46448.0 69 37891.0 70 29334.0 71 23277.5 72 17221.0 73 14237.5 74 11254.0 75 8701.5 76 6149.0 77 4773.5 78 3398.0 79 2719.0 80 2040.0 81 1552.0 82 1064.0 83 818.5 84 573.0 85 441.0 86 309.0 87 228.0 88 147.0 89 124.0 90 101.0 91 70.0 92 39.0 93 34.0 94 29.0 95 23.5 96 18.0 97 18.5 98 19.0 99 26.0 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08280269951094336 2 0.09073242782415743 3 0.09920668683699133 4 0.10067011309221967 5 0.09236601992301698 6 0.05458920263688991 7 0.050062791196299916 8 0.05530389918014096 9 0.05809461901569269 10 0.04165659852091849 11 0.045332180743352476 12 0.05469130214306863 13 0.04768046938546307 14 0.04982455901521623 15 0.04928002831559638 16 0.0519005823075169 17 0.051390084776623296 18 0.05278544469439916 19 0.06051097399525577 20 0.06636467901616916 21 0.07677882864639878 22 0.08018214551902283 23 0.05581439671103458 24 0.07334147860504849 25 0.08018214551902283 26 0.07933131630086682 27 0.07402214197957328 28 0.09335298181607794 29 0.08287076584839585 30 0.07694899449002998 31 0.053466108068923976 32 0.04910986247196518 33 0.052308980332231796 34 0.04761240304801059 35 0.054623235805616156 36 0.054010638768543826 37 0.05693749127900051 38 0.04642124214259217 39 0.051934615476243146 40 0.053568207575102694 41 0.04063560345913127 42 0.036381457368351196 43 0.037776817286127064 44 0.0351222301254803 45 0.02756686666825488 46 0.024469848314166986 47 0.029370624610745633 48 0.02470808049525067 49 0.02412951662690458 50 0.024844213170155633 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2938310.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.52360924831918 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5560762052628 42.70702127762501 2 12.89294452440323 14.575115167152491 3 4.708795448723563 7.984743419217432 4 2.240640143272523 5.06596271697736 5 1.2411166373180846 3.507619591967765 6 0.7623345900555711 2.5853941490867216 7 0.5096584610153415 2.0165414990371615 8 0.3421007414052305 1.5469414904599628 9 0.27730616110888145 1.4106910583402874 >10 1.3753956406118817 13.656252155913313 >50 0.07036025833011036 2.7112708583835876 >100 0.022361354017448515 1.8968674609223746 >500 9.09834475296288E-4 0.33557915491658136 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7016584363120296E-4 2 0.0 0.0 0.0 0.0 1.7016584363120296E-4 3 0.0 0.0 0.0 0.0 1.7016584363120296E-4 4 0.0 0.0 0.0 0.0 2.0419901235744356E-4 5 0.0 0.0 0.0 0.0 2.0419901235744356E-4 6 6.806633745248118E-5 0.0 0.0 0.0 2.7226534980992473E-4 7 1.0209950617872178E-4 0.0 0.0 0.0 3.0629851853616533E-4 8 1.0209950617872178E-4 0.0 0.0 0.0 3.403316872624059E-4 9 1.0209950617872178E-4 0.0 0.0 0.0 3.743648559886465E-4 10 1.3613267490496236E-4 0.0 0.0 0.0 4.424311934411277E-4 11 1.3613267490496236E-4 0.0 0.0 0.0 4.424311934411277E-4 12 1.3613267490496236E-4 0.0 0.0 0.0 7.48729711977293E-4 13 1.3613267490496236E-4 0.0 0.0 0.0 8.167960494297742E-4 14 1.7016584363120296E-4 0.0 0.0 0.0 8.508292181560148E-4 15 1.7016584363120296E-4 3.403316872624059E-5 0.0 0.0 0.0010550282305134583 16 1.7016584363120296E-4 3.403316872624059E-5 0.0 0.0 0.0011571277366921801 17 2.3823218108368416E-4 3.403316872624059E-5 0.0 0.0 0.0011571277366921801 18 2.3823218108368416E-4 3.403316872624059E-5 0.0 0.0 0.0012932604115971425 19 3.403316872624059E-4 3.403316872624059E-5 0.0 0.0 0.0012932604115971425 20 3.403316872624059E-4 3.403316872624059E-5 0.0 0.0 0.0013613267490496237 21 3.403316872624059E-4 3.403316872624059E-5 0.0 0.0 0.001429393086502105 22 3.743648559886465E-4 3.403316872624059E-5 0.0 3.403316872624059E-5 0.001429393086502105 23 3.743648559886465E-4 3.403316872624059E-5 0.0 6.806633745248118E-5 0.0015995589301333079 24 5.785638683460901E-4 3.403316872624059E-5 0.0 1.7016584363120296E-4 0.0015995589301333079 25 5.785638683460901E-4 3.403316872624059E-5 0.0 2.3823218108368416E-4 0.0016335920988595485 26 5.785638683460901E-4 3.403316872624059E-5 0.0 3.743648559886465E-4 0.0017356916050382703 27 5.785638683460901E-4 3.403316872624059E-5 0.0 8.167960494297742E-4 0.0017697247737645109 28 6.806633745248119E-4 3.403316872624059E-5 0.0 0.0015314925926808267 0.0017697247737645109 29 7.827628807035336E-4 3.403316872624059E-5 0.0 0.003028952016635413 0.0017697247737645109 30 7.827628807035336E-4 3.403316872624059E-5 0.0 0.004900776296578645 0.0017697247737645109 31 7.827628807035336E-4 3.403316872624059E-5 0.0 0.010856580823670749 0.001837791111216992 32 7.827628807035336E-4 3.403316872624059E-5 0.0 0.01783338041255007 0.0019058574486694733 33 8.167960494297742E-4 3.403316872624059E-5 0.0 0.026273606256657738 0.0019398906173957139 34 8.167960494297742E-4 3.403316872624059E-5 0.0 0.036313391030898715 0.002076023292300676 35 8.848623868822554E-4 3.403316872624059E-5 0.0 0.051151852595539615 0.002076023292300676 36 9.18895555608496E-4 3.403316872624059E-5 0.0 0.06871296765827976 0.002076023292300676 37 9.18895555608496E-4 3.403316872624059E-5 0.0 0.09035806296816878 0.002076023292300676 38 0.0010209950617872177 3.403316872624059E-5 0.0 0.11639343704374283 0.0021440896297531572 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3775 0.0 23.200996 1 GGTATCA 1890 0.0 21.30737 1 TATCAAC 4925 0.0 17.738218 2 ATCAACG 5065 0.0 16.987827 3 TCAACGC 5050 0.0 16.907269 4 CAACGCA 5030 0.0 16.84239 5 AACGCAG 5205 0.0 16.441303 6 TGCGATA 195 8.949428E-10 15.792971 13 CTAGTAC 160 3.8170583E-7 15.129105 3 GTCTTAT 390 0.0 14.670648 1 GTACATG 9190 0.0 14.343392 1 CGCACGA 345 0.0 14.026103 35 GTGTAGG 805 0.0 13.941671 1 TACATGG 9405 0.0 13.827843 2 GCGATAC 225 6.7357178E-9 13.688174 14 TAGATTG 165 8.706367E-6 13.336043 5 ACGCAGA 6390 0.0 12.979199 7 GTATAAT 340 0.0 12.944689 1 ACATGGG 9795 0.0 12.895791 3 TATACTG 365 0.0 12.660107 5 >>END_MODULE