FastQCFastQC Report
Mon 6 Feb 2017
SRR2077126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938310
Sequences flagged as poor quality0
Sequence length50
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.024.410561
GTATCAA41400.022.9050731
TATCAAC57300.016.3577862
TCAACGC58500.015.8720694
ATCAACG59650.015.6398423
CAACGCA59100.015.4883445
ACGTCGT1301.3704157E-515.230597523
AACGCAG62100.014.7391116
TACCGTT1057.156354E-414.66857
CTATACG1503.2499265E-614.6684994
CGTTATT1057.17947E-414.66225944
CGAATCG2451.0004442E-1014.3647437
GTACATG85750.014.2914351
CGCGTAA950.006234657513.8974210
CGTGCCG3850.013.71693410
TACATGG89700.013.6625672
GTGCCGA3550.013.63666711
ACCGTGC7300.013.2621578
GTACCGA3700.013.0824936
GTATAGA4550.013.055911
Produced by FastQC (version 0.11.5)