FastQCFastQC Report
Mon 6 Feb 2017
SRR2077125_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077125_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081966
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37800.021.8893091
GGTATCA20100.021.020411
GCGATAC1951.8189894E-1218.05042314
TATCAAC48950.016.4531962
ATCAACG48900.016.425823
TCAACGC48750.016.250134
CAACGCA49300.016.0675355
AACGCAG51550.015.5329376
GTACATG88350.014.2719751
CGTATAC950.00623127813.8985883
TGCGATA2401.1314114E-913.74778413
TACATGG88400.013.7406962
GTATAGC6000.013.5702071
GTGTAGC9950.013.4909571
GTGTACG3001.2732926E-1113.20344451
TACACCG3203.6379788E-1213.0646355
ACGCAGA60550.013.0061797
GTATATA5250.012.9938651
GTATTAC3051.8189894E-1112.9869941
CGTGCCG2552.8012437E-912.93867110
Produced by FastQC (version 0.11.5)