##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077125_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3081966 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.74131771732719 31.0 30.0 34.0 18.0 34.0 2 28.77387063971504 31.0 30.0 34.0 16.0 34.0 3 28.871134853531803 31.0 30.0 34.0 18.0 34.0 4 32.25062379013915 35.0 33.0 37.0 20.0 37.0 5 32.173343898018345 35.0 33.0 37.0 19.0 37.0 6 32.306907993144634 36.0 33.0 37.0 22.0 37.0 7 32.088380598617896 35.0 33.0 37.0 18.0 37.0 8 32.03435858799221 35.0 33.0 37.0 18.0 37.0 9 33.3861655190226 38.0 33.0 39.0 17.0 39.0 10 33.29853833559488 38.0 33.0 39.0 17.0 39.0 11 33.353107075159166 38.0 33.0 39.0 17.0 39.0 12 33.24729863989415 38.0 33.0 39.0 17.0 39.0 13 33.18194230565814 38.0 33.0 39.0 17.0 39.0 14 34.0838562138583 39.0 33.0 41.0 17.0 41.0 15 34.120775504986106 39.0 33.0 41.0 17.0 41.0 16 34.07632433323405 39.0 33.0 41.0 17.0 41.0 17 33.91215444946505 39.0 33.0 41.0 16.0 41.0 18 33.878284186133136 39.0 33.0 41.0 16.0 41.0 19 34.00016288304284 39.0 33.0 41.0 16.0 41.0 20 33.9941521094003 39.0 33.0 41.0 16.0 41.0 21 33.92286190048819 39.0 33.0 41.0 15.0 41.0 22 33.84630881716411 39.0 33.0 41.0 11.0 41.0 23 33.799000053861725 39.0 33.0 41.0 11.0 41.0 24 33.80590992892199 39.0 33.0 41.0 10.0 41.0 25 33.70519629353471 39.0 33.0 41.0 10.0 41.0 26 33.65496731631692 39.0 33.0 41.0 10.0 41.0 27 33.598949177245956 39.0 33.0 41.0 10.0 41.0 28 33.47696210795317 39.0 33.0 41.0 9.0 41.0 29 33.46767842344789 39.0 33.0 41.0 9.0 41.0 30 33.3062314769209 38.0 33.0 40.0 8.0 41.0 31 33.30400140689417 38.0 33.0 40.0 8.0 41.0 32 33.183491965842585 38.0 32.0 40.0 8.0 41.0 33 33.13148230707282 38.0 32.0 40.0 2.0 41.0 34 33.052374361040975 38.0 32.0 40.0 2.0 41.0 35 32.979412491896404 38.0 32.0 40.0 2.0 41.0 36 32.88402954477759 38.0 32.0 40.0 2.0 41.0 37 32.78557518155619 38.0 32.0 40.0 2.0 41.0 38 32.700865291829956 38.0 32.0 40.0 2.0 41.0 39 32.59464056384788 38.0 32.0 40.0 2.0 41.0 40 32.51881104463839 38.0 32.0 40.0 2.0 41.0 41 32.41058597012427 38.0 31.0 40.0 2.0 41.0 42 32.26235169369163 38.0 31.0 40.0 2.0 41.0 43 32.16255760121948 38.0 31.0 40.0 2.0 41.0 44 32.00620869925236 38.0 31.0 40.0 2.0 41.0 45 31.862144163822702 38.0 31.0 40.0 2.0 41.0 46 31.725395088719342 38.0 31.0 40.0 2.0 41.0 47 31.561944875446386 38.0 31.0 40.0 2.0 41.0 48 31.397695172497038 38.0 30.0 40.0 2.0 41.0 49 31.218762309512822 37.0 30.0 40.0 2.0 41.0 50 30.994365285016123 37.0 30.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5692427682847061 1101 2 0.564378256446016 1101 3 0.7363322830215324 1101 4 0.8211432694325609 1101 5 0.7697780796476295 1101 6 0.8241778782201052 1101 7 0.8115624009631404 1101 8 0.7887836947016282 1101 9 1.040175702621795 1101 10 0.964351597580297 1101 11 0.8618677580076195 1101 12 1.083220932576232 1101 13 0.9767026071658975 1101 14 1.1899941538995975 1101 15 1.089381238329743 1101 16 1.0837370115493883 1101 17 1.3337898627973175 1101 18 1.319955992852492 1101 19 1.0168043706286483 1101 20 1.2411505735843988 1101 21 1.2554721853156394 1101 22 1.3115449197824134 1101 23 1.2672255903801073 1101 24 1.126757778083217 1101 25 1.0724035111495738 1101 26 1.1492872048286458 1101 27 1.1297350032694808 1101 28 1.2354481355464912 1101 29 1.3168225652822372 1101 30 1.4755233037317055 1101 31 1.2815865454482491 1101 32 1.2989069267573186 1101 33 1.4219963337920376 1101 34 1.547058813637392 1101 35 1.5443524963495392 1101 36 1.530436192003755 1101 37 1.435437897913495 1101 38 1.487572428229008 1101 39 1.726287564343238 1101 40 1.6396865730794516 1101 41 1.61457022802864 1101 42 1.6114549498054984 1101 43 1.6397232126884163 1101 44 1.5289288820632052 1101 45 1.697134166679561 1101 46 1.5241321331960656 1101 47 1.4056685680419108 1101 48 1.480744931020805 1101 49 1.4548769204430059 1101 50 1.4980997156949876 1102 1 0.7528775117399853 1102 2 0.7303409079520371 1102 3 0.7958958500626672 1102 4 0.8336528602073585 1102 5 0.9325996382817507 1102 6 1.0552366167564102 1102 7 1.0347856715443413 1102 8 1.0577307662409225 1102 9 1.1780569307323319 1102 10 1.2392415464483122 1102 11 1.152555634438265 1102 12 1.2069428404456986 1102 13 1.240061049985954 1102 14 1.5060966290250022 1102 15 1.42200441442251 1102 16 1.4800724806665144 1102 17 1.7268361651157988 1102 18 1.637218823076445 1102 19 1.5793720787596683 1102 20 1.4937842808542001 1102 21 1.4467861886934443 1102 22 1.4562176282637012 1102 23 1.3415230111381433 1102 24 1.363610490010707 1102 25 1.489697748982195 1102 26 1.5994515303668493 1102 27 1.4443346608402265 1102 28 1.5108013964431208 1102 29 1.5163528455702462 1102 30 1.5496039256687553 1102 31 1.5748554234628926 1102 32 1.6225286357229933 1102 33 1.6570218599441873 1102 34 1.6505953568548932 1102 35 1.702586585291499 1102 36 1.7517504377447182 1102 37 1.6136027041939514 1102 38 1.5795643512279085 1102 39 1.7456450762231839 1102 40 1.7213598018115093 1102 41 1.6399242359349913 1102 42 1.5248644050893247 1102 43 1.3351130671012044 1102 44 1.3500608906329035 1102 45 1.2988622823657963 1102 46 1.3940007159260581 1102 47 1.3088931267998731 1102 48 1.2277372208844817 1102 49 1.306211528008916 1102 50 1.27716362287455 1103 1 0.42147622580345256 1103 2 0.5250021913874576 1103 3 0.5301269933643091 1103 4 0.6034345179548879 1103 5 0.544853746494546 1103 6 0.6293414148172047 1103 7 0.5245218621112429 1103 8 0.6666086916882179 1103 9 0.6778324134445057 1103 10 0.6629174938108306 1103 11 0.670142012038113 1103 12 0.6588344228940812 1103 13 0.7276516911464199 1103 14 0.804265468617281 1103 15 0.7058028516785129 1103 16 0.6582167432292749 1103 17 0.9392683968565194 1103 18 0.7254409960538908 1103 19 0.8659008471025373 1103 20 0.7218927113649656 1103 21 0.550688448186186 1103 22 0.6453617800257234 1103 23 0.6756186665180408 1103 24 0.5260984784977936 1103 25 0.6976696030025451 1103 26 0.6784171439081277 1103 27 0.680914488237299 1103 28 0.6779993882931095 1103 29 0.6650158499808967 1103 30 0.9018132340971903 1103 31 0.7545256260338533 1103 32 0.9335579745924747 1103 33 0.8600927251165302 1103 34 0.7360293685349717 1103 35 0.7661481422297953 1103 36 0.7702638805958344 1103 37 0.8496440788747535 1103 38 0.9856634783643017 1103 39 0.9823721519713615 1103 40 0.8513718343830377 1103 41 0.8796701458650062 1103 42 0.7340263951224415 1103 43 0.764303133134554 1103 44 0.7752428533092512 1103 45 0.6994699693452873 1103 46 0.7271252449419272 1103 47 0.6564954571143744 1103 48 0.58096576652434 1103 49 0.6382184922565628 1103 50 0.7253482402329432 1104 1 0.5661228851143711 1104 2 0.6612835638217405 1104 3 0.6503201847511697 1104 4 0.5910446518488826 1104 5 0.7536186847653923 1104 6 0.6371414778810589 1104 7 0.6910341484040927 1104 8 0.8242751316180303 1104 9 0.7632992622502002 1104 10 0.5524385117724435 1104 11 0.6233830714767308 1104 12 0.6819031946872656 1104 13 0.6414743093319402 1104 14 0.7395208099226878 1104 15 0.7369654990042349 1104 16 0.7353789926156296 1104 17 0.8246685022354967 1104 18 0.7754810625520108 1104 19 0.7569230385253434 1104 20 0.6905465110982263 1104 21 0.7519352964842412 1104 22 0.7414164820388081 1104 23 0.8188866345622827 1104 24 0.7500450762609887 1104 25 0.8551112225203781 1104 26 0.7402165657717248 1104 27 0.8795520313977931 1104 28 0.7516143386844192 1104 29 0.8569419140045014 1104 30 0.8119107070970202 1104 31 0.8922751664700996 1104 32 0.7878401071318422 1104 33 0.6921513237328369 1104 34 0.6792240772360287 1104 35 0.8150606570652172 1104 36 0.852121167204686 1104 37 0.8380577147033321 1104 38 0.7810542042305855 1104 39 0.8213376220432025 1104 40 0.8490659665319527 1104 41 0.9441732045980302 1104 42 0.7237418088507752 1104 43 0.7592438434052227 1104 44 0.8060180804251402 1104 45 0.8768942437490139 1104 46 0.8958458732772847 1104 47 0.8069965183144134 1104 48 0.773181639773842 1104 49 0.8563206110622446 1104 50 0.8236960337903092 1105 1 0.6183876780091673 1105 2 0.5689599224091282 1105 3 0.4556987524335625 1105 4 0.5909140355583489 1105 5 0.6031635953732177 1105 6 0.5283803002100242 1105 7 0.6035322317519984 1105 8 0.6750691754831877 1105 9 0.7029356994441187 1105 10 0.620921360765692 1105 11 0.6574566064486618 1105 12 0.6771500174839886 1105 13 0.7115986603597477 1105 14 0.8038117398330868 1105 15 0.9325251210216479 1105 16 0.7218162945165858 1105 17 0.8252859501563492 1105 18 0.6855653040604963 1105 19 0.6164472976818232 1105 20 0.6965530607893271 1105 21 0.7375593460067691 1105 22 0.6595884954549334 1105 23 0.6746929685965313 1105 24 0.7115253483619952 1105 25 0.6401144487347565 1105 26 0.7690169779209128 1105 27 0.7420447517191988 1105 28 0.7436752997787579 1105 29 0.6555696271020182 1105 30 0.690711547168533 1105 31 0.6009251323282996 1105 32 0.8248454699183867 1105 33 0.7983339294662457 1105 34 0.7951562332310758 1105 35 0.7816471222900532 1105 36 0.7409197124927402 1105 37 0.8582694858626283 1105 38 0.7942317322456987 1105 39 0.9399542107600212 1105 40 0.9047051069151948 1105 41 0.8476476859101822 1105 42 0.8924636224671687 1105 43 0.8733827249154515 1105 44 0.9218648919119232 1105 45 0.9772500271672087 1105 46 1.0432070042847847 1105 47 0.8182843493473229 1105 48 0.9282590501223105 1105 49 0.8651634459108664 1105 50 0.9016009724858414 1106 1 0.48403527128649415 1106 2 0.4676279176175626 1106 3 0.4912407864561956 1106 4 0.4211203373167791 1106 5 0.44586787047580856 1106 6 0.45208729378612134 1106 7 0.4436529338642856 1106 8 0.6404701070365277 1106 9 0.6160209701030226 1106 10 0.6201036936787432 1106 11 0.44244502893123894 1106 12 0.6080383774747347 1106 13 0.7520925819663375 1106 14 0.7138860689116271 1106 15 0.701953706392267 1106 16 0.6555781770161957 1106 17 0.8294629075123652 1106 18 0.533298361611223 1106 19 0.6421376593001824 1106 20 0.5696271081036741 1106 21 0.7193961022240742 1106 22 0.7130538014681562 1106 23 0.6537134208988888 1106 24 0.6469421626006877 1106 25 0.7009204989517457 1106 26 0.7274970568778514 1106 27 0.7188273037471902 1106 28 0.7579133637596911 1106 29 0.7084327605401839 1106 30 0.6903065308214877 1106 31 0.6839933869486785 1106 32 0.7352576799098358 1106 33 0.6899782045373541 1106 34 0.7793395027345866 1106 35 0.6861884054662113 1106 36 0.8164159086761771 1106 37 0.6996524600646765 1106 38 0.6742079003471559 1106 39 0.7100686322068412 1106 40 0.819467661556196 1106 41 0.76448829080595 1106 42 0.6658504437735786 1106 43 0.7498571465511645 1106 44 0.740034983036665 1106 45 0.873764021717669 1106 46 0.7900542361483822 1106 47 0.7045729797687272 1106 48 0.785612465844121 1106 49 0.824443168491193 1106 50 0.7918977378644172 1107 1 0.08255664862214473 1107 2 0.033551429852980874 1107 3 -0.0449311219646944 1107 4 -0.11739873415942981 1107 5 -0.1320462661506312 1107 6 -0.09955413652995304 1107 7 -0.02951089939024598 1107 8 0.02514041183698623 1107 9 0.007490648827705115 1107 10 -0.05761599766217529 1107 11 -0.18802997035060542 1107 12 -0.09901658194757346 1107 13 -0.009782477718154325 1107 14 -0.19662581635750342 1107 15 -0.08407643437385559 1107 16 -0.09908734526096197 1107 17 -0.1982936232328143 1107 18 0.03236677886774686 1107 19 -0.1431721915332247 1107 20 0.11629312260807012 1107 21 0.04149345607567767 1107 22 -0.2940144729337746 1107 23 -0.4114228771330559 1107 24 -0.3386383743928292 1107 25 -0.3983134044800991 1107 26 -0.12582978588815763 1107 27 -0.12280001967793197 1107 28 -0.17042968601504072 1107 29 -0.09702455660399778 1107 30 -0.28621578283284776 1107 31 -0.2182477609190201 1107 32 -0.13705158785944604 1107 33 -0.1526596598486023 1107 34 -0.14383958645869654 1107 35 -0.19909524241895582 1107 36 -0.21484979853557462 1107 37 -0.2238235182369479 1107 38 -0.15908692435705518 1107 39 0.020099508984664283 1107 40 0.0027480843436791247 1107 41 -0.08125301195028811 1107 42 -0.07484052214861947 1107 43 -0.09934376057549343 1107 44 0.04091349995755422 1107 45 0.028966141555589076 1107 46 -0.0997581740337381 1107 47 -0.018952609448234625 1107 48 -0.07678405191992255 1107 49 -0.0581898279692723 1107 50 -0.0471671701380032 1108 1 -0.21186020899626 1108 2 -0.16601977661403566 1108 3 -0.19022920756162165 1108 4 -0.31125525413874655 1108 5 -0.3673626476245424 1108 6 -0.40736842028097797 1108 7 -0.44765342348073744 1108 8 -0.2581304948916845 1108 9 -0.4531093410168836 1108 10 -0.3896206003231555 1108 11 -0.36232495071078574 1108 12 -0.5086703860373447 1108 13 -0.40756477959713067 1108 14 -0.5625622918619442 1108 15 -0.43183898235952967 1108 16 -0.3779674181955812 1108 17 -0.4902644625992849 1108 18 -0.4265279541212976 1108 19 -0.5276598121066769 1108 20 -0.6268345927840713 1108 21 -0.4543768932421415 1108 22 -0.6555054198277972 1108 23 -0.7342278424083801 1108 24 -0.5472110647640136 1108 25 -0.46538319191622435 1108 26 -0.6582845145968719 1108 27 -0.6562539658359583 1108 28 -0.69158958946025 1108 29 -0.8404431454823396 1108 30 -0.6898873413823807 1108 31 -0.731537180153019 1108 32 -0.5306539228979261 1108 33 -0.5694169148048829 1108 34 -0.5713351546037941 1108 35 -0.5368796352992007 1108 36 -0.7828901116879834 1108 37 -0.6488183733137376 1108 38 -0.6254906718123436 1108 39 -0.6806246672841354 1108 40 -0.6082301562608166 1108 41 -0.5595439326030913 1108 42 -0.3739411227391827 1108 43 -0.4448027887423258 1108 44 -0.5450034528774594 1108 45 -0.49992720178566685 1108 46 -0.5181502432239071 1108 47 -0.5815001265301873 1108 48 -0.6042463422638207 1108 49 -0.6156842968407368 1108 50 -0.6242570940238643 1109 1 0.03069573389438318 1109 2 0.019137619272591166 1109 3 0.03883298142998015 1109 4 -0.05699985611968117 1109 5 -0.060500872225958346 1109 6 -0.17457457132132248 1109 7 -0.10048426933465038 1109 8 -0.18210347188661657 1109 9 -0.24276789792487108 1109 10 -0.3189348812577393 1109 11 -0.17750727058687232 1109 12 -0.2249057557766676 1109 13 -0.077668458455868 1109 14 -0.04385944793270369 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49 -1.6424323083281926 2216 50 -1.7118508772028846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 181967.0 3 8939.0 4 16150.0 5 12669.0 6 10603.0 7 9137.0 8 8050.0 9 7312.0 10 7047.0 11 7095.0 12 7444.0 13 7658.0 14 8115.0 15 8664.0 16 9252.0 17 10045.0 18 10634.0 19 12065.0 20 14409.0 21 17784.0 22 21648.0 23 24838.0 24 27287.0 25 30002.0 26 34117.0 27 38056.0 28 43648.0 29 50259.0 30 59418.0 31 70641.0 32 85934.0 33 106455.0 34 135165.0 35 175113.0 36 230638.0 37 315913.0 38 484788.0 39 773007.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.31461741424802 16.503186523239293 12.687444692510654 25.494751370002028 2 16.538750833033465 18.654136197885883 38.479585646232614 26.32752732284804 3 20.496531689963582 22.456824355113895 27.273742991356915 29.77290096356561 4 13.805996811782114 15.001578518634965 34.723382473116466 36.469042196466454 5 15.638962170181408 36.06633671951905 32.74417447554934 15.550526634750197 6 35.23875195275216 33.737778660817824 16.392534675554206 14.630934710875806 7 30.949779249448124 30.09599402674977 19.63196338137904 19.322263342423064 8 27.74988637101487 34.015713265372376 18.67846243750406 19.555937926108694 9 27.161509077698554 15.740640337953618 17.985309978033793 39.112540606314035 10 15.803261106319495 28.173274717255765 31.95216793032291 24.071296246101827 11 37.00212267083216 21.876689023042292 21.628681603595197 19.492506702530356 12 24.681471073489394 23.516404815966478 28.360745574877917 23.441378535666217 13 29.308008642831357 20.46825940959697 24.561917784027905 25.661814163543767 14 23.295333362982767 19.971134003215774 25.285665266758883 31.447867367042576 15 25.128706719433797 27.51049926747787 22.136549397117893 25.22424461597044 16 25.358513988976593 26.54123519569504 23.59696320617695 24.503287609151418 17 24.234143978724454 26.42893131416491 24.978476429905232 24.3584482772054 18 24.32219836471854 26.154893994986246 25.426367209185003 24.096540431110213 19 25.243533268053476 25.70595752307089 25.418399310145993 23.632109898729638 20 25.326758178979226 25.224221616642485 25.361207606667406 24.087812597710883 21 25.532657608656827 25.051517133302898 25.13104153258357 24.284783725456705 22 25.39358323188166 24.84368216146776 24.971204395051995 24.79153021159858 23 25.021329589887504 25.047756205060146 25.052853230345285 24.87806097470707 24 24.68070224886018 25.171245164627525 25.129909285116582 25.01814330139571 25 25.04504931637723 24.968833244625664 25.14149658229209 24.844620856705017 26 24.800625295705185 25.247677953228166 25.262258228032252 24.689438523034397 27 24.945586649465675 25.291208432600023 25.028420280504353 24.734784637429954 28 24.938657728801395 25.241609790080332 25.0497964459644 24.769936035153872 29 24.839110462220106 25.39525017844142 25.12159670485757 24.64404265448091 30 25.0508273467849 25.3787656155475 24.98484353035499 24.58556350731261 31 25.083724415195185 25.0009089893856 24.96445202224167 24.950914573177542 32 24.945656337691645 25.316290502909368 24.942702172058397 24.79535098734059 33 25.027854429763334 25.109372463721062 24.90049670486641 24.962276401649188 34 25.118514029016016 25.087576860036414 25.018722992874064 24.77518611807351 35 25.072429703255732 25.194823240357533 25.008213677688794 24.72453337869794 36 25.1409057248906 25.253299162957827 24.864045170344884 24.741749941806688 37 25.136973048223343 24.92175751452518 25.02684918484966 24.91442025240182 38 25.09827141641663 25.065127435522623 25.080417166179895 24.75618398188086 39 25.16697112607996 25.311663744354085 24.823111082831247 24.69825404673471 40 25.191119733737455 24.96753529238365 25.068727786023814 24.772617187855083 41 25.031835617523335 25.15164725583956 25.015410613733575 24.80110651290353 42 25.26568698193027 25.095860246099456 24.84209391950823 24.79635885246205 43 24.97041343291818 24.920231499063554 25.108787031897663 25.000568036120608 44 25.066150532385194 25.120551068340834 24.891328762303413 24.92196963697055 45 25.09524214451101 25.048700234397263 24.828161685427318 25.02789593566441 46 25.095548305770155 24.85463285952971 24.87374901173712 25.176069822963008 47 24.965344392834737 25.11409330188499 24.91889890104968 25.001663404230595 48 24.89442370205739 25.124497595729434 25.06033394954667 24.920744752666508 49 25.113422270976915 24.956664400222643 24.925183005888968 25.004730322911477 50 25.1472990893648 25.034451467084 24.96924845495799 24.849000988593207 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1472.0 1 1061.0 2 650.0 3 645.0 4 640.0 5 645.0 6 650.0 7 636.0 8 622.0 9 682.0 10 742.0 11 880.5 12 1019.0 13 1477.0 14 1935.0 15 2508.5 16 3082.0 17 3268.0 18 3454.0 19 3549.5 20 3645.0 21 4061.0 22 4477.0 23 5463.0 24 6449.0 25 8224.5 26 10000.0 27 13312.5 28 16625.0 29 21871.0 30 27117.0 31 35257.5 32 43398.0 33 53892.0 34 64386.0 35 76730.5 36 89075.0 37 102624.5 38 116174.0 39 129684.5 40 143195.0 41 156565.5 42 169936.0 43 181711.0 44 193486.0 45 206133.5 46 218781.0 47 227782.0 48 236783.0 49 241792.5 50 246802.0 51 249285.0 52 251768.0 53 248936.5 54 246105.0 55 237273.5 56 228442.0 57 214156.5 58 199871.0 59 182809.0 60 165747.0 61 146051.0 62 126355.0 63 109213.5 64 92072.0 65 77565.0 66 63058.0 67 52168.0 68 41278.0 69 33590.0 70 25902.0 71 20339.0 72 14776.0 73 12187.0 74 9598.0 75 7621.0 76 5644.0 77 4306.0 78 2968.0 79 2318.5 80 1669.0 81 1280.0 82 891.0 83 711.0 84 531.0 85 407.0 86 283.0 87 219.0 88 155.0 89 117.5 90 80.0 91 72.0 92 64.0 93 47.0 94 30.0 95 24.5 96 19.0 97 18.5 98 18.0 99 32.5 100 47.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0840697139423342 2 0.09273301522469748 3 0.1002282309408994 4 0.10155855061347206 5 0.093673973041883 6 0.05509470253727653 7 0.05081172212801829 8 0.05730108638447017 9 0.0577553418824218 10 0.043965442837461545 11 0.04584735847183259 12 0.055548958035228156 13 0.0484755509956956 14 0.04948140245544565 15 0.049092040600058534 16 0.052304275907002214 17 0.052628744119824815 18 0.053212786902905485 19 0.061454279508599385 20 0.06778140965863996 21 0.07813194564768074 22 0.08121439366949537 23 0.056392575388566915 24 0.07517928491099513 25 0.08316120294643095 26 0.07991652081820501 27 0.07459524212791445 28 0.09510163317830242 29 0.08189577691642283 30 0.07861864796691462 31 0.052628744119824815 32 0.050941509413147325 33 0.05405640425624423 34 0.049611189740574685 35 0.056392575388566915 36 0.055646298499074945 37 0.058339384665502474 38 0.0475994868210746 39 0.05331012736675226 40 0.05509470253727653 41 0.0420835272030905 42 0.03825480229178388 43 0.038157461827937104 44 0.03614575890843702 45 0.028293628158130236 46 0.026022350668372073 47 0.030337777898912576 48 0.025373414242726884 49 0.02534096742144462 50 0.026606393451452742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3081966.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.33908129464373 #Duplication Level Percentage of deduplicated Percentage of total 1 79.05040493066274 49.2792961934709 2 11.638004983437208 14.510050775399217 3 3.974661543555677 7.433302472472343 4 1.7775985546001536 4.4325544321784065 5 0.9827766682780329 3.06326973091317 6 0.5872832426276141 2.1966418683086886 7 0.4001381590110297 1.7460971656574362 8 0.2684754169983926 1.3389208676700939 9 0.2125034349101323 1.192254201382839 >10 1.046815431663296 11.461799675047331 >50 0.04834063860835618 2.04036841076609 >100 0.01278800858395901 1.2120004354141711 >500 2.0898706331528444E-4 0.093443771319337 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 6.489364256451888E-5 0.0 0.0 0.0 0.0 3 6.489364256451888E-5 0.0 0.0 0.0 0.0 4 6.489364256451888E-5 0.0 0.0 3.244682128225944E-5 0.0 5 6.489364256451888E-5 0.0 0.0 3.244682128225944E-5 0.0 6 6.489364256451888E-5 0.0 0.0 3.244682128225944E-5 0.0 7 2.2712774897581608E-4 0.0 0.0 3.244682128225944E-5 0.0 8 2.2712774897581608E-4 0.0 0.0 3.244682128225944E-5 0.0 9 2.2712774897581608E-4 0.0 0.0 3.244682128225944E-5 3.244682128225944E-5 10 2.595745702580755E-4 0.0 0.0 3.244682128225944E-5 3.244682128225944E-5 11 2.595745702580755E-4 0.0 0.0 3.244682128225944E-5 3.244682128225944E-5 12 2.595745702580755E-4 0.0 0.0 3.244682128225944E-5 3.244682128225944E-5 13 2.595745702580755E-4 0.0 0.0 6.489364256451888E-5 3.244682128225944E-5 14 2.92021391540335E-4 0.0 0.0 6.489364256451888E-5 6.489364256451888E-5 15 2.92021391540335E-4 0.0 0.0 6.489364256451888E-5 1.9468092769355666E-4 16 2.92021391540335E-4 0.0 0.0 6.489364256451888E-5 1.9468092769355666E-4 17 3.5691503410485384E-4 0.0 0.0 6.489364256451888E-5 1.9468092769355666E-4 18 3.5691503410485384E-4 0.0 0.0 6.489364256451888E-5 2.2712774897581608E-4 19 6.813832469274482E-4 0.0 0.0 6.489364256451888E-5 2.2712774897581608E-4 20 6.813832469274482E-4 0.0 0.0 6.489364256451888E-5 2.2712774897581608E-4 21 6.813832469274482E-4 0.0 0.0 9.734046384677833E-5 2.2712774897581608E-4 22 7.138300682097077E-4 0.0 0.0 9.734046384677833E-5 2.2712774897581608E-4 23 7.138300682097077E-4 0.0 0.0 9.734046384677833E-5 3.244682128225944E-4 24 8.111705320564861E-4 0.0 0.0 1.2978728512903775E-4 3.244682128225944E-4 25 8.111705320564861E-4 0.0 0.0 1.9468092769355666E-4 3.5691503410485384E-4 26 8.436173533387455E-4 0.0 0.0 3.893618553871133E-4 3.5691503410485384E-4 27 8.436173533387455E-4 0.0 0.0 6.164896043629294E-4 3.5691503410485384E-4 28 0.001038298281032302 0.0 0.0 0.0012654260300081182 3.5691503410485384E-4 29 0.00116808556616134 0.0 0.0 0.002660639345145274 3.5691503410485384E-4 30 0.00116808556616134 0.0 0.0 0.0046398954433631 3.5691503410485384E-4 31 0.00116808556616134 0.0 0.0 0.011680855661613398 3.893618553871133E-4 32 0.00116808556616134 0.0 0.0 0.019662773697049222 3.893618553871133E-4 33 0.00116808556616134 0.0 0.0 0.029721288294549647 3.893618553871133E-4 34 0.0012329792087258588 0.0 0.0 0.04111012256462271 3.893618553871133E-4 35 0.001395213315137156 3.244682128225944E-5 0.0 0.05743087366959921 3.893618553871133E-4 36 0.0015574474215484533 3.244682128225944E-5 0.0 0.07654205140485001 3.893618553871133E-4 37 0.0015574474215484533 3.244682128225944E-5 0.0 0.10133142286449623 3.893618553871133E-4 38 0.0017196815279597504 3.244682128225944E-5 0.0 0.13063090248237652 3.893618553871133E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3780 0.0 21.889309 1 GGTATCA 2010 0.0 21.02041 1 GCGATAC 195 1.8189894E-12 18.050423 14 TATCAAC 4895 0.0 16.453196 2 ATCAACG 4890 0.0 16.42582 3 TCAACGC 4875 0.0 16.25013 4 CAACGCA 4930 0.0 16.067535 5 AACGCAG 5155 0.0 15.532937 6 GTACATG 8835 0.0 14.271975 1 CGTATAC 95 0.006231278 13.898588 3 TGCGATA 240 1.1314114E-9 13.747784 13 TACATGG 8840 0.0 13.740696 2 GTATAGC 600 0.0 13.570207 1 GTGTAGC 995 0.0 13.490957 1 GTGTACG 300 1.2732926E-11 13.2034445 1 TACACCG 320 3.6379788E-12 13.064635 5 ACGCAGA 6055 0.0 13.006179 7 GTATATA 525 0.0 12.993865 1 GTATTAC 305 1.8189894E-11 12.986994 1 CGTGCCG 255 2.8012437E-9 12.938671 10 >>END_MODULE