FastQCFastQC Report
Mon 6 Feb 2017
SRR2077125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081966
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41200.021.7875251
GGTATCA21000.020.9534531
CGTGCCG3200.017.19033410
TATCAAC56700.015.59944252
TTAACGT1004.9589004E-415.39504243
TCAACGC58450.015.2086674
ATCAACG58500.015.1949273
CAACGCA59000.015.0300855
AACGCAG61350.014.5607796
GTATAGA5450.014.1291651
GTGCCGA3600.014.05787311
GTACATG86850.013.9834451
CGACGTT950.00625605913.890264541
GCACCGT6550.013.4366766
TACATGG87600.013.41232
TCGAACG1658.753999E-613.329041544
TTATACT6450.013.304524
ATTAACC6550.013.1007593
TATACTG7250.013.0508515
TACCGTA1557.1659175E-512.77671412
Produced by FastQC (version 0.11.5)