FastQCFastQC Report
Mon 6 Feb 2017
SRR2077124_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077124_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396595
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45600.018.1930371
CCGTACG800.001990804416.4981949
TCGCTTA850.002978333315.5318328
GGTATCA27350.015.528471
GCGATAC2001.2787496E-915.39967414
TCAACGC54900.014.6700324
ATCAACG55550.014.6574753
CAACGCA55450.014.5622755
TATCAAC56850.014.5156322
GTCTACG1554.5606503E-614.1970141
AACGCAG58200.013.9099496
CGTGCCG2855.456968E-1213.89280610
CGCACGA2855.456968E-1213.8911735
TATACTG7500.013.7880015
GTACATG84750.013.7095421
TAGCGCG1150.001424070913.39220629
GCACCGT5100.013.3718736
TGCGATA2351.2403689E-813.10514113
GTGTAGC11600.013.0894021
TACATGG85050.013.0662862
Produced by FastQC (version 0.11.5)