##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077124_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3396595 Sequences flagged as poor quality 0 Sequence length 50 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.532344598045984 31.0 30.0 34.0 25.0 34.0 2 29.672956298881672 31.0 30.0 34.0 25.0 34.0 3 29.813013032168982 31.0 30.0 34.0 25.0 34.0 4 33.24069487236483 37.0 35.0 37.0 27.0 37.0 5 33.24572255449943 37.0 35.0 37.0 27.0 37.0 6 33.317385204889014 37.0 35.0 37.0 27.0 37.0 7 33.1449277879759 37.0 35.0 37.0 26.0 37.0 8 33.11456944381064 37.0 35.0 37.0 26.0 37.0 9 34.53192476583166 39.0 35.0 39.0 27.0 39.0 10 34.47364787382658 39.0 35.0 39.0 27.0 39.0 11 34.5271470399032 39.0 35.0 39.0 27.0 39.0 12 34.42652980411265 39.0 35.0 39.0 26.0 39.0 13 34.37833006290123 39.0 35.0 39.0 26.0 39.0 14 35.39235499080697 40.0 36.0 41.0 25.0 41.0 15 35.432082423721404 40.0 36.0 41.0 26.0 41.0 16 35.41176560643821 40.0 36.0 41.0 26.0 41.0 17 35.263519495259224 40.0 35.0 41.0 25.0 41.0 18 35.24655103125336 39.0 35.0 41.0 25.0 41.0 19 35.36632804323153 40.0 36.0 41.0 25.0 41.0 20 35.34314659239621 40.0 35.0 41.0 25.0 41.0 21 35.3022191341623 40.0 35.0 41.0 25.0 41.0 22 35.23840169346066 39.0 35.0 41.0 25.0 41.0 23 35.20726845561511 39.0 35.0 41.0 25.0 41.0 24 35.21367251615221 39.0 35.0 41.0 25.0 41.0 25 35.12508585804313 39.0 35.0 41.0 24.0 41.0 26 35.08191321014133 39.0 35.0 41.0 24.0 41.0 27 35.01608758182827 39.0 35.0 41.0 24.0 41.0 28 34.92145369112302 39.0 35.0 41.0 24.0 41.0 29 34.90377834272264 39.0 35.0 41.0 24.0 41.0 30 34.75796319549431 39.0 34.0 41.0 23.0 41.0 31 34.75876753042385 39.0 34.0 41.0 23.0 41.0 32 34.62998826766217 39.0 34.0 41.0 22.0 41.0 33 34.595430129291245 39.0 34.0 41.0 22.0 41.0 34 34.52788748732186 39.0 34.0 41.0 21.0 41.0 35 34.47449990357991 39.0 34.0 41.0 21.0 41.0 36 34.39532826256884 39.0 34.0 41.0 19.0 41.0 37 34.31309208192322 39.0 34.0 41.0 19.0 41.0 38 34.23364958141904 39.0 34.0 41.0 18.0 41.0 39 34.131451350543706 39.0 34.0 41.0 16.0 41.0 40 34.0770754240644 39.0 34.0 41.0 16.0 41.0 41 33.97461074988334 39.0 33.0 41.0 15.0 41.0 42 33.84433174988481 38.0 33.0 40.0 15.0 41.0 43 33.78034119463757 38.0 33.0 40.0 15.0 41.0 44 33.645935120319024 38.0 33.0 40.0 9.0 41.0 45 33.51672483766831 38.0 33.0 40.0 9.0 41.0 46 33.389904006806816 38.0 33.0 40.0 7.0 41.0 47 33.246820124271515 38.0 33.0 40.0 2.0 41.0 48 33.10197212208109 38.0 33.0 40.0 2.0 41.0 49 32.94378664515493 38.0 33.0 40.0 2.0 41.0 50 32.74600416004852 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.187000853346909 1101 2 1.4006019093052586 1101 3 1.6496787852930943 1101 4 1.773757177773426 1101 5 1.9053368978455794 1101 6 1.8530853797637903 1101 7 1.967206204981622 1101 8 2.053812657378515 1101 9 2.229871008377991 1101 10 2.3256877912848424 1101 11 2.3040927859615223 1101 12 2.32689523745708 1101 13 2.352194422501192 1101 14 2.71992292859413 1101 15 2.655910105271907 1101 16 2.682868669308199 1101 17 2.7594578023917578 1101 18 2.812074979768653 1101 19 2.735751557248747 1101 20 2.7719229217955643 1101 21 2.786330850866129 1101 22 2.81485913564714 1101 23 2.8329256466900645 1101 24 2.795315073713816 1101 25 2.874627455505383 1101 26 2.863630299153371 1101 27 2.811584599814573 1101 28 2.94157223322987 1101 29 2.8224514413306068 1101 30 3.0290222308617913 1101 31 2.9459458409226755 1101 32 2.952022188688602 1101 33 2.8918474625343293 1101 34 2.9717706097212115 1101 35 3.1131248911787495 1101 36 3.165317878217465 1101 37 3.173854126175776 1101 38 3.184718679965158 1101 39 3.123685305155824 1101 40 3.2344845422024733 1101 41 3.149427808478869 1101 42 3.1583052658037616 1101 43 3.227276127369109 1101 44 3.1000749557500953 1101 45 3.142926744493721 1101 46 3.1676212974910385 1101 47 3.204984012838395 1101 48 3.253410325657441 1101 49 3.0363921020443883 1101 50 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-0.9442677496212681 2216 19 -0.9435518318863032 2216 20 -1.04997965469817 2216 21 -1.2165528500596068 2216 22 -0.9668914574471543 2216 23 -1.0288143819801405 2216 24 -1.140334196099964 2216 25 -1.307085148258949 2216 26 -1.1559746292015305 2216 27 -1.2070330514885796 2216 28 -1.3100163423267404 2216 29 -1.5327986166965673 2216 30 -1.3158556317037693 2216 31 -1.5887870123715757 2216 32 -1.3853888580347942 2216 33 -1.3580104578063086 2216 34 -1.4492753160752798 2216 35 -1.4387338041616005 2216 36 -1.5111696170338647 2216 37 -1.5673021077689526 2216 38 -1.6069543979442642 2216 39 -1.611470711893304 2216 40 -1.7467751235609654 2216 41 -1.654345294814732 2216 42 -1.6067862019839794 2216 43 -1.8449515751979106 2216 44 -1.832922721858715 2216 45 -2.1022957930022663 2216 46 -2.24495260566486 2216 47 -2.0229659846840384 2216 48 -2.142938189064079 2216 49 -2.1485427488627096 2216 50 -2.2037647110842435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135726.0 3 6810.0 4 12659.0 5 10013.0 6 8390.0 7 7275.0 8 6576.0 9 6024.0 10 5994.0 11 6006.0 12 6218.0 13 6543.0 14 6816.0 15 7445.0 16 7884.0 17 8551.0 18 9339.0 19 10782.0 20 13267.0 21 16934.0 22 20911.0 23 24077.0 24 26310.0 25 28770.0 26 31749.0 27 36397.0 28 42109.0 29 49028.0 30 57652.0 31 69628.0 32 85651.0 33 107305.0 34 138847.0 35 182811.0 36 246419.0 37 345124.0 38 558211.0 39 1046344.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.690564188604846 17.344311189333542 12.968874171600014 24.9962504504616 2 16.118681228009173 19.015046589616496 39.41331266612838 25.452959516245954 3 19.974979223029724 23.113893162166463 27.97352336156644 28.937604253237375 4 13.337596831258317 15.476350111769467 36.23502679667388 34.951026260298335 5 14.923959947062086 36.61960583000752 33.73563281054538 14.72080141238501 6 34.01024185361997 35.08194248796211 16.886155999738417 14.021659658679505 7 29.979100604300356 30.847419683136295 20.418370848656856 18.755108863906493 8 26.694153208404686 34.956494535046666 19.267219323690853 19.0821329328578 9 26.51270869137685 16.25475828417201 18.65692218772959 38.57561083672155 10 14.989589478790608 28.832416380790914 33.068080302245306 23.109913838173167 11 36.15288637644925 22.5383488393876 22.405271488987687 18.903493295175462 12 23.98606880230054 24.141606152916193 29.373876736060478 22.49844830872279 13 28.59286825614612 21.209541589664045 25.20915159359346 24.988438560596375 14 22.469913729519302 20.657844816819562 26.30507372226137 30.56716773139977 15 24.40360400271938 28.490390857794594 22.776748126302692 24.329257013183337 16 24.397269708218623 27.437829952969274 24.552742693093734 23.612157645718366 17 23.334206231075967 27.368319349786425 25.776837260734947 23.520637158402664 18 23.316561569438843 27.147664084378636 26.178856855189697 23.356917490992824 19 24.165251889291202 26.74575868744011 26.276650620248414 22.812338803020275 20 24.41542869348103 26.22480169105787 26.196047815103153 23.16372180035795 21 24.59618907258358 25.966233713428228 26.075898569731027 23.361678644257164 22 24.325212249571436 25.822553642112485 25.904053234614523 23.94818087370156 23 24.078521156218663 25.906484670593628 25.945163156635793 24.06983101655192 24 23.89057582398415 25.915467561249688 26.01988534079264 24.174071273973517 25 24.102952741578907 25.896354825184183 26.03926125918542 23.96143117405149 26 23.99601638820179 26.094601940832167 26.11484411025058 23.794537560715458 27 24.04284982761191 26.243236028786544 25.8999330011084 23.813981142493148 28 23.961894456236884 26.157557411894857 25.950774691115182 23.92977344075308 29 23.957870171887226 26.347908462355562 25.937836551706866 23.756384814050346 30 24.144046407502326 26.286810527252445 25.94260819450231 23.62653487074292 31 24.235299177349557 25.870820285033485 25.78672005877295 24.107160478844005 32 23.912063104272335 26.265041360704284 25.88900550209447 23.93389003292891 33 24.157268949297688 25.966891677732622 25.77774923736213 24.09809013560756 34 24.192910341699204 25.976827312371775 25.978535734746806 23.851726611182215 35 24.34352658385203 26.069702155988843 25.809976051114518 23.776795209044607 36 24.149989203920992 26.111370171184866 25.82752019220261 23.91112043269153 37 24.222630184183828 25.840262860522962 25.964634961109418 23.972471994183792 38 24.09348829098861 26.03531908175336 25.91363985541056 23.957552771847475 39 24.19074337436418 26.26639103299003 25.82336625777208 23.71949933487371 40 24.374366486693162 25.836208107850954 26.045619144098296 23.74380626135758 41 24.130879466067388 26.039052363862353 25.879443977279585 23.950624192790674 42 24.17700227461424 26.038781245020694 25.904360173074494 23.879856307290577 43 24.02042832928605 25.788321157134085 25.998377161269232 24.19287335231063 44 24.09178890225355 26.09714563067908 25.830016057226402 23.98104940984097 45 24.19934233427159 25.970109958458565 25.71012997232338 24.120417734946457 46 24.15875132445904 25.888013343832807 25.734732425407703 24.218502906300447 47 24.130648743748488 25.952783296393132 25.80382485691685 24.11274310294153 48 23.87359132656487 26.056291574050842 26.00452094796198 24.06559615142231 49 24.231132921425445 25.843858030481105 25.78828845410268 24.13672059399077 50 24.15024073113708 25.863408896612388 25.954494762047382 24.03185561020315 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1674.0 1 1181.0 2 688.0 3 660.0 4 632.0 5 642.0 6 652.0 7 662.5 8 673.0 9 730.5 10 788.0 11 945.0 12 1102.0 13 1638.5 14 2175.0 15 2907.5 16 3640.0 17 4120.0 18 4600.0 19 5014.0 20 5428.0 21 6562.0 22 7696.0 23 9993.5 24 12291.0 25 16505.5 26 20720.0 27 27456.5 28 34193.0 29 43057.5 30 51922.0 31 63744.0 32 75566.0 33 89105.5 34 102645.0 35 115887.0 36 129129.0 37 142367.0 38 155605.0 39 167849.0 40 180093.0 41 193077.5 42 206062.0 43 215973.0 44 225884.0 45 236399.0 46 246914.0 47 254027.0 48 261140.0 49 261698.0 50 262256.0 51 260462.5 52 258669.0 53 251922.0 54 245175.0 55 232897.5 56 220620.0 57 204429.0 58 188238.0 59 170929.5 60 153621.0 61 134511.5 62 115402.0 63 98473.5 64 81545.0 65 68266.0 66 54987.0 67 44789.5 68 34592.0 69 27794.5 70 20997.0 71 16544.0 72 12091.0 73 9731.5 74 7372.0 75 5697.0 76 4022.0 77 3139.0 78 2256.0 79 1744.5 80 1233.0 81 977.0 82 721.0 83 539.5 84 358.0 85 278.5 86 199.0 87 166.5 88 134.0 89 96.0 90 58.0 91 50.5 92 43.0 93 33.5 94 24.0 95 20.5 96 17.0 97 18.5 98 20.0 99 26.5 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08402532536260579 2 0.09229831640216157 3 0.10054186619246627 4 0.10157230991625436 5 0.09379982011396708 6 0.05585004982931436 7 0.051816598681915266 8 0.057822613529137264 9 0.058558644760414474 10 0.043337518897601866 11 0.046428850068966125 12 0.05629166856808068 13 0.04972626998508801 14 0.04978515248359018 15 0.05013844747460324 16 0.05278815990720118 17 0.052169893672928326 18 0.05305313115046097 19 0.06088450345125044 20 0.06683163579997027 21 0.07707719053934896 22 0.07987410921820234 23 0.05720434729486441 24 0.07469244935001082 25 0.08143449542851001 26 0.07919696048542732 27 0.07386809437098035 28 0.09356429011995837 29 0.08243549790304702 30 0.07834316425714577 31 0.054054133624997974 32 0.05022677122235651 33 0.05296480740270771 34 0.04857806126429557 35 0.05490792985327953 36 0.05428966361900668 37 0.05720434729486441 38 0.047194322549494426 39 0.051551627438655476 40 0.054054133624997974 41 0.04151216144403439 42 0.03809697653090816 43 0.03856803651892557 44 0.036477707822098304 45 0.02793974553928272 46 0.025584445599195666 47 0.02994175048835672 48 0.024936738115671726 49 0.02508394436192717 50 0.026438241827477227 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3396595.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.353159936123944 #Duplication Level Percentage of deduplicated Percentage of total 1 75.96474614509192 45.84712473601788 2 13.140767189924738 15.861736477937956 3 4.810320339160761 8.709540983200782 4 2.1649463367663424 5.226454100639389 5 1.1808148398672174 3.5632953442727375 6 0.7058966644742598 2.5561856573634856 7 0.4491530199589066 1.8975462834561074 8 0.3053364569057333 1.4742416014368913 9 0.2087043447050193 1.1336370025811378 >10 1.0090973095082931 10.474957356368607 >50 0.04802106428667824 1.947453811109702 >100 0.011853240172692252 1.1410698940355615 >500 2.4503512743438553E-4 0.10005996669818622 >1k 9.801405005836513E-5 0.06669678488152636 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.832374775326466E-5 2 0.0 0.0 0.0 0.0 8.832374775326466E-5 3 0.0 0.0 0.0 0.0 8.832374775326466E-5 4 0.0 2.9441249251088222E-5 0.0 0.0 8.832374775326466E-5 5 0.0 2.9441249251088222E-5 0.0 0.0 8.832374775326466E-5 6 0.0 2.9441249251088222E-5 0.0 0.0 1.1776499700435289E-4 7 0.0 2.9441249251088222E-5 0.0 0.0 1.1776499700435289E-4 8 0.0 2.9441249251088222E-5 0.0 0.0 1.1776499700435289E-4 9 0.0 2.9441249251088222E-5 0.0 0.0 1.472062462554411E-4 10 0.0 2.9441249251088222E-5 0.0 0.0 1.7664749550652933E-4 11 0.0 2.9441249251088222E-5 0.0 5.8882498502176444E-5 1.7664749550652933E-4 12 0.0 2.9441249251088222E-5 0.0 5.8882498502176444E-5 2.0608874475761755E-4 13 0.0 2.9441249251088222E-5 0.0 5.8882498502176444E-5 2.3552999400870578E-4 14 0.0 2.9441249251088222E-5 0.0 5.8882498502176444E-5 2.3552999400870578E-4 15 0.0 2.9441249251088222E-5 0.0 5.8882498502176444E-5 2.3552999400870578E-4 16 0.0 2.9441249251088222E-5 0.0 8.832374775326466E-5 2.3552999400870578E-4 17 0.0 2.9441249251088222E-5 0.0 8.832374775326466E-5 2.3552999400870578E-4 18 0.0 2.9441249251088222E-5 0.0 1.1776499700435289E-4 2.64971243259794E-4 19 0.0 2.9441249251088222E-5 0.0 1.1776499700435289E-4 2.64971243259794E-4 20 0.0 5.8882498502176444E-5 0.0 1.1776499700435289E-4 2.64971243259794E-4 21 2.9441249251088222E-5 5.8882498502176444E-5 0.0 2.3552999400870578E-4 2.64971243259794E-4 22 2.9441249251088222E-5 5.8882498502176444E-5 0.0 2.64971243259794E-4 3.2385374176197045E-4 23 2.9441249251088222E-5 5.8882498502176444E-5 0.0 5.593837357706763E-4 4.121774895152351E-4 24 2.9441249251088222E-5 5.8882498502176444E-5 0.0 7.94913729779382E-4 4.4161873876632336E-4 25 2.9441249251088222E-5 5.8882498502176444E-5 0.0 9.421199760348231E-4 4.4161873876632336E-4 26 2.9441249251088222E-5 5.8882498502176444E-5 0.0 0.0013542974655500583 4.4161873876632336E-4 27 2.9441249251088222E-5 5.8882498502176444E-5 0.0 0.001737033705814205 4.7105998801741156E-4 28 2.9441249251088222E-5 8.832374775326466E-5 0.0 0.0026202711833468516 5.005012372684998E-4 29 2.9441249251088222E-5 8.832374775326466E-5 0.0 0.004092333645901263 5.005012372684998E-4 30 2.9441249251088222E-5 8.832374775326466E-5 0.0 0.006388751087486144 5.005012372684998E-4 31 2.9441249251088222E-5 1.1776499700435289E-4 0.0 0.013719622151007112 5.29942486519588E-4 32 2.9441249251088222E-5 1.1776499700435289E-4 0.0 0.023081939412853168 5.888249850217644E-4 33 2.9441249251088222E-5 1.1776499700435289E-4 0.0 0.03376911289099819 5.888249850217644E-4 34 2.9441249251088222E-5 1.1776499700435289E-4 0.0 0.04878415000905319 6.182662342728527E-4 35 2.9441249251088222E-5 1.1776499700435289E-4 0.0 0.06800928577001379 6.182662342728527E-4 36 2.9441249251088222E-5 1.1776499700435289E-4 0.0 0.09097346018586261 6.182662342728527E-4 37 2.9441249251088222E-5 1.1776499700435289E-4 0.0 0.11864823448188554 6.182662342728527E-4 38 2.9441249251088222E-5 1.472062462554411E-4 0.0 0.14985595868803905 6.182662342728527E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4560 0.0 18.193037 1 CCGTACG 80 0.0019908044 16.498194 9 TCGCTTA 85 0.0029783333 15.53183 28 GGTATCA 2735 0.0 15.52847 1 GCGATAC 200 1.2787496E-9 15.399674 14 TCAACGC 5490 0.0 14.670032 4 ATCAACG 5555 0.0 14.657475 3 CAACGCA 5545 0.0 14.562275 5 TATCAAC 5685 0.0 14.515632 2 GTCTACG 155 4.5606503E-6 14.197014 1 AACGCAG 5820 0.0 13.909949 6 CGTGCCG 285 5.456968E-12 13.892806 10 CGCACGA 285 5.456968E-12 13.89117 35 TATACTG 750 0.0 13.788001 5 GTACATG 8475 0.0 13.709542 1 TAGCGCG 115 0.0014240709 13.392206 29 GCACCGT 510 0.0 13.371873 6 TGCGATA 235 1.2403689E-8 13.105141 13 GTGTAGC 1160 0.0 13.089402 1 TACATGG 8505 0.0 13.066286 2 >>END_MODULE