FastQCFastQC Report
Mon 6 Feb 2017
SRR2077124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3396595
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55000.020.3217891
GGTATCA30350.018.6309641
CGCTATC2700.017.9219633
CGTGCCG2250.017.60440310
CGCTACG2850.016.21028128
ATCAACG70250.015.5975623
TATCAAC70450.015.5845132
TCAACGC70950.015.4441294
CGCGATC1158.220406E-515.30524523
CAACGCA71300.015.1838275
TATCTCG2900.015.16883436
AACGCAG74200.014.7068456
TATACCG1402.754568E-514.145565
TACGCTA3600.014.05410231
GTGCCGA2204.8876245E-914.00432711
GCGATAC1658.69518E-613.33784714
ACGCTAT3500.013.19766332
TATACTG8250.013.0691655
GTACATG81050.013.00299551
ATAGGAC6800.012.9426983
Produced by FastQC (version 0.11.5)