FastQCFastQC Report
Mon 6 Feb 2017
SRR2077123_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077123_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587974
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42850.019.6176241
GGTATCA23950.019.0194851
TCAACGC52950.015.5842934
TATCAAC53200.015.38742
GTACATG98100.015.3209481
CAACGCA54200.015.2223285
ATCAACG54100.015.2127693
GTATAGG5750.014.5428551
TACATGG100650.014.5392442
AACGCAG57350.014.4605356
GTATTAG3850.014.2893741
GTATATG7100.013.9472341
ACATGGG105350.013.6190553
GCACCGT5300.012.4515046
GTATTAA6550.012.4306641
GTATAAC3750.012.3231551
GTATAGC6000.012.10311
ACGCAGA67950.012.0104947
CATGGGG78350.011.8801834
TACCGTA2056.2182717E-611.8036217
Produced by FastQC (version 0.11.5)