##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077123_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3587974
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.051208007638852	31.0	28.0	33.0	15.0	34.0
2	28.03450080741945	31.0	28.0	34.0	15.0	34.0
3	28.183435275729423	31.0	28.0	34.0	16.0	34.0
4	31.51897170938251	35.0	32.0	37.0	16.0	37.0
5	31.460693973813633	35.0	32.0	37.0	16.0	37.0
6	31.44586304137098	35.0	32.0	37.0	16.0	37.0
7	31.289296410732074	35.0	32.0	37.0	11.0	37.0
8	31.251092120511466	35.0	32.0	37.0	10.0	37.0
9	32.53729904397301	37.0	32.0	39.0	10.0	39.0
10	32.457455934742	37.0	32.0	39.0	10.0	39.0
11	32.486980117470196	37.0	32.0	39.0	10.0	39.0
12	32.37281401704695	37.0	32.0	39.0	10.0	39.0
13	32.31046824754026	37.0	32.0	39.0	10.0	39.0
14	33.13172559221444	38.0	32.0	40.0	8.0	41.0
15	33.1588406159019	38.0	32.0	40.0	8.0	41.0
16	33.10840658265639	38.0	32.0	40.0	8.0	41.0
17	32.926318306654395	38.0	32.0	40.0	8.0	41.0
18	32.8952849156655	38.0	32.0	40.0	8.0	41.0
19	33.019368590742296	38.0	32.0	40.0	2.0	41.0
20	33.00871383125965	38.0	32.0	40.0	2.0	41.0
21	32.93870189694797	38.0	32.0	40.0	2.0	41.0
22	32.85344208179881	38.0	32.0	40.0	2.0	41.0
23	32.79188282858237	38.0	32.0	40.0	2.0	41.0
24	32.808841702866296	38.0	32.0	40.0	2.0	41.0
25	32.6977528265255	38.0	32.0	40.0	2.0	41.0
26	32.64867025234854	38.0	32.0	40.0	2.0	41.0
27	32.58079935919268	38.0	32.0	40.0	2.0	41.0
28	32.458201480835704	38.0	31.0	40.0	2.0	41.0
29	32.44099260474017	38.0	31.0	40.0	2.0	41.0
30	32.27172493446162	38.0	31.0	40.0	2.0	41.0
31	32.2705972785756	38.0	31.0	40.0	2.0	41.0
32	32.14462200673695	38.0	31.0	40.0	2.0	41.0
33	32.09749624718574	38.0	31.0	40.0	2.0	41.0
34	32.013931539080275	38.0	30.0	40.0	2.0	41.0
35	31.94018908721189	38.0	30.0	40.0	2.0	41.0
36	31.837541464904707	38.0	30.0	40.0	2.0	41.0
37	31.7410480120536	38.0	30.0	40.0	2.0	41.0
38	31.646390971617965	38.0	30.0	40.0	2.0	41.0
39	31.527285314776528	38.0	30.0	40.0	2.0	41.0
40	31.451486827942453	38.0	30.0	40.0	2.0	41.0
41	31.344852833381736	38.0	30.0	40.0	2.0	41.0
42	31.18932495051525	38.0	30.0	40.0	2.0	41.0
43	31.076586675377246	37.0	30.0	40.0	2.0	41.0
44	30.91764460946484	37.0	30.0	40.0	2.0	41.0
45	30.757863908712828	37.0	30.0	40.0	2.0	41.0
46	30.608531444207788	37.0	29.0	40.0	2.0	41.0
47	30.441573433921203	37.0	29.0	40.0	2.0	41.0
48	30.26618559666263	37.0	29.0	40.0	2.0	41.0
49	30.073571324652853	37.0	28.0	40.0	2.0	41.0
50	29.84604654325812	36.0	28.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	2.6022607884734583
1101	2	2.480364215656266
1101	3	2.6602157307395586
1101	4	3.0092097661677712
1101	5	3.0489136617507953
1101	6	2.8468054621153733
1101	7	3.0088761627488694
1101	8	3.2240793722550016
1101	9	3.7081170078520245
1101	10	3.7488915462740593
1101	11	3.6844195207587447
1101	12	3.937870359698607
1101	13	3.784384795282122
1101	14	4.371364572357365
1101	15	4.334575329178641
1101	16	4.342791585727021
1101	17	4.44300829352327
1101	18	4.4141795500567085
1101	19	4.388507419894843
1101	20	4.409599817450939
1101	21	4.4540625275311285
1101	22	4.4230410703711485
1101	23	4.65481311848815
1101	24	4.482544346678544
1101	25	4.5165908487990265
1101	26	4.581784463830672
1101	27	4.6635104096688025
1101	28	4.609059050803353
1101	29	4.570280461671402
1101	30	4.653933935846773
1101	31	4.625582516835259
1101	32	4.817095207180458
1101	33	4.83881349046834
1101	34	4.876755807210557
1101	35	4.836214594185705
1101	36	4.847269878214345
1101	37	4.859677096878244
1101	38	4.95452152249608
1101	39	4.9114295363282565
1101	40	4.87199161443705
1101	41	4.880777288186696
1101	42	4.927398298087908
1101	43	4.81356115989691
1101	44	4.79932505066596
1101	45	4.8525975977160485
1101	46	4.790150365675466
1101	47	4.8480325282654455
1101	48	4.833285037900513
1101	49	4.682555766464478
1101	50	4.863199548005138
1102	1	1.6536899065365844
1102	2	1.5676210046917696
1102	3	1.6973469686772482
1102	4	1.8763620817448654
1102	5	1.9160791830616262
1102	6	1.8638891078770392
1102	7	2.0670894205325254
1102	8	2.106882801365334
1102	9	2.287871265251745
1102	10	2.4106402044426787
1102	11	2.2795279237281676
1102	12	2.365546549953933
1102	13	2.4038072086976
1102	14	2.778218412584735
1102	15	2.5937060955750084
1102	16	2.741418253836656
1102	17	2.8812043001093883
1102	18	2.9227961302741363
1102	19	2.8770905155249693
1102	20	2.8266721900252705
1102	21	2.8075804705802767
1102	22	2.757895253303417
1102	23	2.782185125940046
1102	24	2.717313948496681
1102	25	2.9083850635281223
1102	26	2.963442730362324
1102	27	2.7442181854625005
1102	28	2.8876792899752957
1102	29	2.6094319191978954
1102	30	2.7706125843011193
1102	31	2.819540732605354
1102	32	2.8791129402262214
1102	33	2.8838789264894586
1102	34	2.9212624796490942
1102	35	2.9420998421571767
1102	36	2.9605117505683687
1102	37	2.8490894481687974
1102	38	2.9166220329460018
1102	39	2.9708103098106307
1102	40	2.8787457996883674
1102	41	2.9094151779490964
1102	42	2.839977242608363
1102	43	2.6927913751310477
1102	44	2.6477724428878524
1102	45	2.725854891790341
1102	46	2.620117460852086
1102	47	2.63464069031075
1102	48	2.687128937109204
1102	49	2.6128175749671705
1102	50	2.7238407926680885
1103	1	0.4082886680343236
1103	2	0.3858687856526508
1103	3	0.3798028991231277
1103	4	0.4983599214104828
1103	5	0.6394269335333149
1103	6	0.37365563819962233
1103	7	0.4561553181857505
1103	8	0.5968351219356975
1103	9	0.5569245870454012
1103	10	0.6592437771198902
1103	11	0.6593070299103161
1103	12	0.6183177490736966
1103	13	0.6743069779786595
1103	14	0.7418486838043563
1103	15	0.7224759275266379
1103	16	0.6180741933771117
1103	17	0.8065305307284376
1103	18	0.6164341766102481
1103	19	0.7138858629787492
1103	20	0.6031290552742306
1103	21	0.5468262842801366
1103	22	0.5542913041704196
1103	23	0.6235982440450343
1103	24	0.567621896782839
1103	25	0.7318721026400183
1103	26	0.6807971183831256
1103	27	0.5731227214937888
1103	28	0.8144350089350212
1103	29	0.8435900261545726
1103	30	0.9083654339968632
1103	31	0.7381446183745055
1103	32	0.8530579881181097
1103	33	0.8293555306942864
1103	34	0.8472329714792579
1103	35	0.6929611059006078
1103	36	0.8672116198897548
1103	37	0.897288051331472
1103	38	0.8474318825202403
1103	39	0.9895543414955341
1103	40	0.9095582583620967
1103	41	0.8431734590445323
1103	42	0.9829245112166447
1103	43	0.8160249588633839
1103	44	0.7972756330710737
1103	45	0.5537126198312308
1103	46	0.653090999365002
1103	47	0.6874937033361199
1103	48	0.6921827255589434
1103	49	0.8275280133778864
1103	50	0.7007130638295642
1104	1	0.2875317703658844
1104	2	0.37793024706698475
1104	3	0.28574932215088467
1104	4	0.307226044285553
1104	5	0.34921191702900956
1104	6	0.2681217862222489
1104	7	0.28559553156961215
1104	8	0.37746226599660915
1104	9	0.37801091286517163
1104	10	0.309577567105606
1104	11	0.27580663100960123
1104	12	0.36980418425610395
1104	13	0.35386981008117147
1104	14	0.5049734191092412
1104	15	0.4217503522152981
1104	16	0.49702329832381764
1104	17	0.5403400837793129
1104	18	0.3703896781018017
1104	19	0.39613963011722575
1104	20	0.4356733595713891
1104	21	0.45575995466873565
1104	22	0.4514050125630291
1104	23	0.49736553847470333
1104	24	0.47657250278540886
1104	25	0.48638818513765614
1104	26	0.42448975965173474
1104	27	0.5836443676452134
1104	28	0.3843749520800941
1104	29	0.4266226609608452
1104	30	0.441168256176816
1104	31	0.46529494180817466
1104	32	0.6145781044596887
1104	33	0.49686057117162363
1104	34	0.49062766778105527
1104	35	0.5668229473549928
1104	36	0.4678962816818917
1104	37	0.4848781938781741
1104	38	0.5068827592489455
1104	39	0.5667785480681147
1104	40	0.5423670993861478
1104	41	0.5638151458024403
1104	42	0.4688394413127668
1104	43	0.549105549480128
1104	44	0.639337752415738
1104	45	0.6567552308106741
1104	46	0.5977677094599443
1104	47	0.5432623389689439
1104	48	0.5777703475014562
1104	49	0.6384127639662438
1104	50	0.7005666134124127
1105	1	0.04839395638082067
1105	2	0.1340186257622875
1105	3	-0.0033264319961539
1105	4	0.047352358342621415
1105	5	-4.1126660639179136E-4
1105	6	0.0698128310904842
1105	7	0.1000561595740308
1105	8	0.13081212576194545
1105	9	0.0939476049729393
1105	10	0.07919019028238239
1105	11	0.0567864907063651
1105	12	-0.04059272440964179
1105	13	0.08088641194502344
1105	14	0.17178649213412456
1105	15	0.18871223170263818
1105	16	0.12958654107035983
1105	17	0.17058722594091336
1105	18	0.029405841089449325
1105	19	0.03223856927115776
1105	20	0.0934411254857963
1105	21	0.1720035172463099
1105	22	0.1747648841917382
1105	23	0.0023478119905817607
1105	24	0.03769218569735244
1105	25	0.09774753560088101
1105	26	0.24505008112855364
1105	27	0.2357393157686758
1105	28	0.22472104711050633
1105	29	0.14196755593913934
1105	30	0.1065498384626764
1105	31	-0.050041381369283044
1105	32	0.07165361173885998
1105	33	0.12617887299501263
1105	34	0.07528242993072354
1105	35	0.09828317077040793
1105	36	0.16872157466604776
1105	37	0.2275815341375207
1105	38	0.1119617226782168
1105	39	0.23217315949123574
1105	40	0.2849936363100376
1105	41	0.29099735485837996
1105	42	0.2741302996586157
1105	43	0.3433326130554626
1105	44	0.2577208364811021
1105	45	0.37421701772256455
1105	46	0.35209612600831974
1105	47	0.14617503689182598
1105	48	0.28916329942394015
1105	49	0.17404196843455466
1105	50	0.29871098996819256
1106	1	0.11229074064209144
1106	2	0.03177743775784947
1106	3	0.013986698191079228
1106	4	0.008792035164340462
1106	5	-0.03689196840225861
1106	6	0.0505368916256117
1106	7	0.008439243604996705
1106	8	0.09335752063471148
1106	9	0.12914328917381113
1106	10	0.07904689138469934
1106	11	0.08723661981892405
1106	12	0.13847640414953588
1106	13	0.23687988622975809
1106	14	0.172087332562036
1106	15	0.02622377921251484
1106	16	0.04256642067789329
1106	17	0.16035713142725427
1106	18	-0.18440019564753385
1106	19	0.011017525303081754
1106	20	-0.09764915084942061
1106	21	0.11844986783912503
1106	22	0.16572058735275874
1106	23	0.06717315396857515
1106	24	0.08531104834714398
1106	25	0.060216538171872
1106	26	0.015452877413309807
1106	27	0.07283762990260101
1106	28	0.10414159714633797
1106	29	-0.0466878494776779
1106	30	0.08278555571995838
1106	31	0.03186442360122044
1106	32	0.19059848036605587
1106	33	0.08676824213646483
1106	34	0.15976252036299599
1106	35	0.06893645161429163
1106	36	0.025283909827276574
1106	37	0.06600157020008979
1106	38	0.08942903358475363
1106	39	0.1274342190642841
1106	40	0.12573159044876192
1106	41	0.11924117071296081
1106	42	0.03869978090591175
1106	43	0.10529417258431906
1106	44	0.19627291244331246
1106	45	0.19462288208428902
1106	46	0.08238657438102948
1106	47	0.10957938072586515
1106	48	0.218768919240226
1106	49	0.23210436805354817
1106	50	0.38698682641323146
1107	1	-0.08480617681931335
1107	2	-0.14558510384144085
1107	3	-0.1787880074577295
1107	4	-0.16974165875139846
1107	5	-0.2844143006094271
1107	6	-0.18611457483692462
1107	7	-0.17742006790219733
1107	8	-0.22793098298116377
1107	9	-0.23423902321368928
1107	10	-0.3420940810018891
1107	11	-0.42727389335401966
1107	12	-0.4966292804461965
1107	13	-0.3515478204892091
1107	14	-0.48833486798723413
1107	15	-0.38993134694094067
1107	16	-0.47835366686984315
1107	17	-0.32544637009268484
1107	18	-0.2682724065887925
1107	19	-0.4518014647688773
1107	20	-0.25709907505440555
1107	21	-0.3806068353640413
1107	22	-0.6830772736504187
1107	23	-0.6216966620538926
1107	24	-0.6011837531785886
1107	25	-0.6032718154184735
1107	26	-0.2490151129366467
1107	27	-0.31515608512672344
1107	28	-0.4087806363911781
1107	29	-0.333162869191284
1107	30	-0.32292254100970297
1107	31	-0.3364420177699259
1107	32	-0.4746130845278387
1107	33	-0.4201211233049804
1107	34	-0.41706596241766647
1107	35	-0.3844840619968224
1107	36	-0.3996914937470244
1107	37	-0.3171219105659233
1107	38	-0.41860400788751306
1107	39	-0.31662063930256323
1107	40	-0.15933055801415463
1107	41	-0.1808197169586947
1107	42	-0.3190783435500286
1107	43	-0.2960187762959272
1107	44	-0.11316568440541985
1107	45	-0.06380052029497563
1107	46	-0.23921811530592052
1107	47	-0.11104737033058143
1107	48	-0.35983769957706
1107	49	-0.3656017212091349
1107	50	-0.26041876916156426
1108	1	-0.26068246799146877
1108	2	-0.29559088620258933
1108	3	-0.15041231194835802
1108	4	-0.37188051763988383
1108	5	-0.32392176858236255
1108	6	-0.43574805217766865
1108	7	-0.4317935445436838
1108	8	-0.30332388852076164
1108	9	-0.5543891220023198
1108	10	-0.4491090373657798
1108	11	-0.3722979918312248
1108	12	-0.516069485315775
1108	13	-0.4816586030641403
1108	14	-0.5961845206695884
1108	15	-0.4698288407992024
1108	16	-0.4039248266589084
1108	17	-0.6552504597451332
1108	18	-0.4361446420397357
1108	19	-0.5007175231624359
1108	20	-0.5575564332009009
1108	21	-0.5357302680546354
1108	22	-0.6531201970475564
1108	23	-0.7582382974417428
1108	24	-0.5041568857544902
1108	25	-0.3199341158124369
1108	26	-0.6462774572073968
1108	27	-0.5865258888848572
1108	28	-0.7323742451339506
1108	29	-0.8642644526251608
1108	30	-0.6571499860002952
1108	31	-0.6255555195091063
1108	32	-0.4940457698580012
1108	33	-0.4697471195273195
1108	34	-0.3966188253560574
1108	35	-0.416832132584549
1108	36	-0.7635645856570363
1108	37	-0.6853180789928501
1108	38	-0.563791302324848
1108	39	-0.6362607815898116
1108	40	-0.6690214055985244
1108	41	-0.44128791751260366
1108	42	-0.42731720100595894
1108	43	-0.3741952561536337
1108	44	-0.3613139429377199
1108	45	-0.4175005535730527
1108	46	-0.3521409871706105
1108	47	-0.48055246004335217
1108	48	-0.43699884950893875
1108	49	-0.5038096481099927
1108	50	-0.39966455716291804
1109	1	-0.45581423852505054
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2210	42	-0.7340059032079189
2210	43	-0.7248058889029316
2210	44	-0.7664520319025527
2210	45	-0.5207949525491777
2210	46	-0.581830722886366
2210	47	-0.5242250717319337
2210	48	-0.4294720895973967
2210	49	-0.286981677225409
2210	50	-0.3961705318306947
2211	1	-0.0897342741261653
2211	2	-0.16081506248941224
2211	3	-0.20954477955399042
2211	4	-0.23130788313517314
2211	5	-0.4068830558726404
2211	6	-0.22419504709753468
2211	7	-0.15751887594558056
2211	8	-0.22461251545946226
2211	9	-0.19815732200496683
2211	10	-0.34854259784301433
2211	11	-0.3297159470662834
2211	12	-0.4092043881714176
2211	13	-0.4071573379924871
2211	14	-0.5146507384139625
2211	15	-0.3749747869497355
2211	16	-0.37421569552633116
2211	17	-0.29367521591968426
2211	18	-0.5148499245289031
2211	19	-0.2867321230251747
2211	20	-0.49836281516341074
2211	21	-0.4285715727052235
2211	22	-0.4408995129969071
2211	23	-0.642807191247563
2211	24	-0.44756331664822113
2211	25	-0.43862737594724166
2211	26	-0.5572688299663398
2211	27	-0.4815174374003206
2211	28	-0.32275042334017456
2211	29	-0.29721959768219364
2211	30	-0.7786219609123606
2211	31	-0.5515333160021001
2211	32	-0.3642488529164929
2211	33	-0.5664655410119863
2211	34	-0.44186718121426694
2211	35	-0.34074835197935727
2211	36	-0.38993604519249914
2211	37	-0.17527991883940786
2211	38	-0.40757500609638697
2211	39	-0.3221762845810723
2211	40	-0.3073515131944653
2211	41	-0.23705985864918233
2211	42	-0.4043160465706386
2211	43	-0.4040435459835159
2211	44	-0.4607349742379867
2211	45	-0.391740478719921
2211	46	-0.41965353823557194
2211	47	-0.5253782280794539
2211	48	-0.5052034962130634
2211	49	-0.4113838836194752
2211	50	-0.28407063692428736
2212	1	-0.2715065411316182
2212	2	-0.29995544328640733
2212	3	-0.2912102452531009
2212	4	-0.22021887639279214
2212	5	-0.2305459467410742
2212	6	-0.20055502659080915
2212	7	-0.21480639633330156
2212	8	-0.11873056482551902
2212	9	-0.31748513034038695
2212	10	-0.39808253699034424
2212	11	-0.29711069005649193
2212	12	-0.4536084538432803
2212	13	-0.3826142664810277
2212	14	-1.329002492236949
2212	15	-0.7373845570807163
2212	16	-0.7373715975295667
2212	17	-0.4471523589628532
2212	18	-0.43376064968897765
2212	19	-0.5591705467648254
2212	20	-0.3996866698808077
2212	21	-0.42010663904294887
2212	22	-0.6091790325880027
2212	23	-0.5676079646816135
2212	24	-0.5072547965630676
2212	25	-0.3329751137784882
2212	26	-0.577418384623499
2212	27	-0.5314424521593608
2212	28	-0.5956191709909042
2212	29	-0.44977826043354696
2212	30	-0.45534020626319105
2212	31	-0.3541546533930031
2212	32	-0.29548292778574137
2212	33	-0.3066745367240671
2212	34	-0.2456382193481339
2212	35	-0.28750757248301895
2212	36	-0.4047042256254265
2212	37	-0.3847708453790375
2212	38	-0.5018882195299348
2212	39	-0.41373115730860377
2212	40	-0.36025235595088034
2212	41	-0.42317421155199497
2212	42	-0.36283889477326525
2212	43	-0.3942547028900627
2212	44	-0.2816303871984793
2212	45	-0.15216621761589622
2212	46	-0.1680832799919827
2212	47	-0.2168758701889324
2212	48	-0.21998037613764865
2212	49	-0.2548363671601983
2212	50	-0.25303030356414524
2213	1	-0.9329141944910937
2213	2	-1.0344534086382104
2213	3	-1.011342466203061
2213	4	-1.2618450815417646
2213	5	-0.9513177631839582
2213	6	-1.0124994273792218
2213	7	-1.2388851224309327
2213	8	-1.2388926381848364
2213	9	-1.150074149144082
2213	10	-1.357240893073321
2213	11	-1.2606443782371244
2213	12	-1.2120560343152036
2213	13	-1.2184581957565932
2213	14	-1.3373292910247798
2213	15	-1.6056656098923163
2213	16	-1.3153696788816802
2213	17	-1.3780648753269311
2213	18	-1.2906443741387683
2213	19	-1.439809027989547
2213	20	-1.4112129983476684
2213	21	-1.475791610475273
2213	22	-1.2117205619460556
2213	23	-1.3225892009039768
2213	24	-1.363461644655878
2213	25	-1.3945307264333593
2213	26	-1.5273436427276508
2213	27	-1.683228427292331
2213	28	-1.6142820824074136
2213	29	-1.7068926827506168
2213	30	-1.764103066428909
2213	31	-1.560614914347969
2213	32	-1.5777285884456127
2213	33	-1.698889488484845
2213	34	-1.7320299832984531
2213	35	-1.7877006014326504
2213	36	-1.8801067272961376
2213	37	-1.7013130161342644
2213	38	-1.5951271615960252
2213	39	-1.7640114567418514
2213	40	-1.613925214012788
2213	41	-1.5597047185246247
2213	42	-1.6364974835498138
2213	43	-1.742371354020726
2213	44	-1.6667454825113737
2213	45	-1.771963659178489
2213	46	-1.795695428176348
2213	47	-1.7029372064958643
2213	48	-1.7408046906894654
2213	49	-1.8246875125697066
2213	50	-1.702210138414074
2214	1	-0.6531026871759096
2214	2	-0.824772154273127
2214	3	-0.7387302111767546
2214	4	-0.8028689034666243
2214	5	-0.726371485055882
2214	6	-0.5813766803994582
2214	7	-0.6114938624910131
2214	8	-0.8409159951078067
2214	9	-0.9586712433540612
2214	10	-0.8465767228235848
2214	11	-0.9061312305804563
2214	12	-0.9255001234801306
2214	13	-0.7957345350621878
2214	14	-0.8897031706430951
2214	15	-0.8375724040240584
2214	16	-0.7140314368823795
2214	17	-0.6350516432172668
2214	18	-0.7263065041078747
2214	19	-0.8652399576065122
2214	20	-0.7909507741421535
2214	21	-0.8489239259987258
2214	22	-0.7497416536195232
2214	23	-0.9480141214185593
2214	24	-1.0020561282998912
2214	25	-1.0559085920895832
2214	26	-1.0988977053085058
2214	27	-1.13885170281376
2214	28	-0.9835377819637721
2214	29	-0.8746364171026038
2214	30	-0.8531641163156571
2214	31	-0.8111447113567358
2214	32	-1.0179988832784446
2214	33	-1.007771670182997
2214	34	-1.1413088154158828
2214	35	-1.1818047670428484
2214	36	-1.1178588771504607
2214	37	-1.0454150766273322
2214	38	-0.9740306985073381
2214	39	-1.0076763646930509
2214	40	-1.004446100554592
2214	41	-0.9888192996363863
2214	42	-0.904540806694893
2214	43	-1.0824640906382363
2214	44	-0.9423708140397338
2214	45	-0.7943768109571003
2214	46	-0.7855959149026042
2214	47	-0.7467328579474
2214	48	-0.6325785495711287
2214	49	-0.7215526372458889
2214	50	-0.743356432356741
2215	1	-0.4483888854422382
2215	2	-0.46223208509968217
2215	3	-0.5506125189605235
2215	4	-0.47041783690746897
2215	5	-0.4170815440005313
2215	6	-0.40447021383599235
2215	7	-0.38402820507333857
2215	8	-0.3717772171759215
2215	9	-0.42998429670694804
2215	10	-0.4825627276367541
2215	11	-0.46525700050602836
2215	12	-0.4666233614322337
2215	13	-0.3476536806701347
2215	14	-0.3832202328457939
2215	15	-0.44103564925167404
2215	16	-0.4572343036593267
2215	17	-0.35275793252488796
2215	18	-0.3303927705858314
2215	19	-0.45314926799335353
2215	20	-0.5462403180830506
2215	21	-0.49747049002930055
2215	22	-0.4490307125261097
2215	23	-0.639708587572521
2215	24	-0.5589507404361953
2215	25	-0.6975481378449331
2215	26	-0.4779421287269301
2215	27	-0.5643667493507962
2215	28	-0.5031018647606587
2215	29	-0.6552553432944848
2215	30	-0.5614713023466713
2215	31	-0.5781179468453175
2215	32	-0.7279243844879311
2215	33	-0.6733909990949662
2215	34	-0.6763861775019571
2215	35	-0.5330150662919024
2215	36	-0.5904853290448813
2215	37	-0.5579963176601694
2215	38	-0.5136013251368396
2215	39	-0.6063889585614994
2215	40	-0.6866427453799631
2215	41	-0.5417564567158095
2215	42	-0.4098953906673408
2215	43	-0.5312582463879458
2215	44	-0.5805388831459091
2215	45	-0.5909277273149414
2215	46	-0.6339723333845377
2215	47	-0.64478325017906
2215	48	-0.7816101184004189
2215	49	-0.7450781875488737
2215	50	-0.7409077172108347
2216	1	-0.3449179012660579
2216	2	-0.32582532687963806
2216	3	-0.3746374870163649
2216	4	-0.26666168917349964
2216	5	-0.3311186317132062
2216	6	-0.44276787704935927
2216	7	-0.4086511776422803
2216	8	-0.45080206802435896
2216	9	-0.2703940784667651
2216	10	-0.44608580947589616
2216	11	-0.5847331697732585
2216	12	-0.7103302902815827
2216	13	-0.598662877544438
2216	14	-0.6069229833539964
2216	15	-0.7441925175849917
2216	16	-0.6933628485150152
2216	17	-0.6664437681540463
2216	18	-0.6413336910628757
2216	19	-0.8321987940847535
2216	20	-0.889890658375208
2216	21	-0.9483977650969848
2216	22	-0.9009271488863959
2216	23	-1.0500315577150623
2216	24	-1.0576386343918678
2216	25	-1.128006476370345
2216	26	-0.9352105711063317
2216	27	-1.0204475722865958
2216	28	-1.031763804473286
2216	29	-1.211204523809105
2216	30	-1.0835611967242933
2216	31	-1.2738946556367345
2216	32	-1.0920874187532057
2216	33	-1.09972729715874
2216	34	-1.1591412670792565
2216	35	-1.250637466806463
2216	36	-1.1687085635004024
2216	37	-1.3226796294232308
2216	38	-1.405619829924042
2216	39	-1.430510201190284
2216	40	-1.5964852885421017
2216	41	-1.5332125304651747
2216	42	-1.5021358903903632
2216	43	-1.6101967750114312
2216	44	-1.6624640620517006
2216	45	-1.689328236940959
2216	46	-1.8248665518517342
2216	47	-1.6440075839166575
2216	48	-1.8298085172239027
2216	49	-1.8929740267682789
2216	50	-1.8304550375411388
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	259247.0
3	12826.0
4	22591.0
5	17317.0
6	14276.0
7	12342.0
8	10910.0
9	9932.0
10	9422.0
11	9453.0
12	9655.0
13	10197.0
14	10751.0
15	11280.0
16	12023.0
17	13039.0
18	14383.0
19	15836.0
20	18789.0
21	23097.0
22	27699.0
23	31553.0
24	35485.0
25	39512.0
26	44066.0
27	50101.0
28	56975.0
29	64866.0
30	74982.0
31	89887.0
32	108250.0
33	132265.0
34	167123.0
35	212946.0
36	275103.0
37	368652.0
38	550331.0
39	740812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.59846627144463	16.491710295345396	12.354682259833991	25.555141173375983
2	16.56294450611021	18.584850157766105	38.286016519482466	26.566188816641223
3	20.351842769390604	22.380749918676322	27.509051618076118	29.758355693856952
4	13.637068713466608	14.670661021450778	34.74907320404688	36.94319706103574
5	15.39835974521625	36.16773235710677	33.061184979330136	15.372722918346845
6	35.348423971604944	33.6843860639335	16.337755746273817	14.62943421818774
7	30.90036902838513	30.202578012109843	19.721965526491463	19.17508743301357
8	27.8900980918452	34.09753410905136	18.71257764732949	19.299790151773948
9	27.234372450950183	15.516656232265586	17.890178044811687	39.358793271972544
10	15.66353377588622	28.083901023403552	32.277892654157405	23.974672546552817
11	37.22193424655853	21.747956201550494	21.683851796415116	19.34625775547585
12	24.489392418738234	23.40430337874825	28.80590567748279	23.300398525030726
13	29.251310296212267	20.38033445875481	24.779683967372815	25.5886712776601
14	23.165806978006138	19.77933635270045	25.380725239265605	31.674131430027813
15	25.059987044750265	27.663194737815132	22.14784432842534	25.12897388900926
16	25.27693680082608	26.609970087339835	23.672020940814093	24.441072171019997
17	24.26004185588025	26.607373160091075	25.059821004067075	24.0727639799616
18	24.30846048889418	26.32801212907752	25.410132977588702	23.953394404439596
19	25.133617510203454	25.908675760884265	25.40875872714419	23.54894800176809
20	25.28325819012617	25.286828027012508	25.392249772562348	24.03766401029898
21	25.261058044448063	25.21277646157082	25.36467796379467	24.16148753018645
22	25.308441558441558	24.761402686660418	25.16180769402419	24.768348060873834
23	24.917755383581863	25.05027260566171	25.3056025057802	24.72636950497623
24	24.745951427731498	25.170123430482704	25.225236783812292	24.858688357973502
25	24.961102529214248	25.00868882190732	25.313397298682926	24.716811350195506
26	24.780415120716594	25.334251559338544	25.288005547290034	24.59732777265483
27	24.784580498866212	25.3993635174447	25.242892564980075	24.57316341870902
28	24.893212940315294	25.38309997143418	25.170167467104676	24.553519621145846
29	24.789363255847423	25.51818898424081	25.251584203652804	24.440863556258964
30	24.977205006001032	25.4512348903846	25.080267107960267	24.4912929956541
31	24.916795940283258	25.108343049187926	25.080624629294018	24.894236381234798
32	24.792642919186157	25.585347184558604	25.05093824809234	24.57107164816289
33	24.89883843010294	25.274957858060095	25.063753191145246	24.76245052069173
34	25.04418214924058	25.237808431554615	25.121476511938525	24.596532907266283
35	24.981086384678814	25.350518859098376	25.14170919964384	24.52668555657897
36	25.054265442227102	25.38719201712401	24.918546065392924	24.639996475255963
37	25.030689251873845	25.083450782582894	25.28484812654736	24.601011838995905
38	25.106662766642973	25.221544303423332	25.09617843221836	24.575614497715335
39	25.068834418715152	25.421692727170313	25.03038083823786	24.47909201587667
40	25.09833163184637	25.116011108511554	25.181124007586003	24.604533252056076
41	24.848768061956022	25.36926913485517	25.076424546861826	24.70553825632698
42	25.089296742324358	25.217996691608324	25.068162738520794	24.624543827546532
43	24.88174043286654	25.015794894443268	25.19705280931645	24.905411863373743
44	24.95789987866243	25.248084026266014	24.992137593233647	24.80187850183791
45	24.946431170117208	25.280528936089304	24.953763255602077	24.81927663819141
46	24.96911129745932	25.080093179060803	24.9348493701827	25.015946153297175
47	24.867170088638773	25.271193821548195	25.0673140562455	24.794322033567536
48	24.78046210288034	25.36305295672246	25.11427121781505	24.742213722582157
49	24.95228022069161	25.11246590896736	25.062062893930364	24.873190976410665
50	24.986304959625766	25.15686222580299	25.117136154488417	24.739696660082824
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1769.0
1	1272.5
2	776.0
3	740.0
4	704.0
5	674.5
6	645.0
7	664.0
8	683.0
9	760.0
10	837.0
11	984.5
12	1132.0
13	1569.5
14	2007.0
15	2588.5
16	3170.0
17	3426.5
18	3683.0
19	3918.5
20	4154.0
21	4727.0
22	5300.0
23	6842.0
24	8384.0
25	11317.0
26	14250.0
27	19329.5
28	24409.0
29	31405.0
30	38401.0
31	48139.0
32	57877.0
33	70187.0
34	82497.0
35	96061.0
36	109625.0
37	122927.5
38	136230.0
39	150376.0
40	164522.0
41	179213.0
42	193904.0
43	208198.5
44	222493.0
45	236755.5
46	251018.0
47	262556.5
48	274095.0
49	277578.0
50	281061.0
51	285553.5
52	290046.0
53	286370.5
54	282695.0
55	272379.5
56	262064.0
57	245590.0
58	229116.0
59	210571.5
60	192027.0
61	169428.5
62	146830.0
63	127232.0
64	107634.0
65	90123.0
66	72612.0
67	60388.0
68	48164.0
69	39183.0
70	30202.0
71	23920.0
72	17638.0
73	14494.0
74	11350.0
75	8809.0
76	6268.0
77	4843.0
78	3418.0
79	2689.0
80	1960.0
81	1457.5
82	955.0
83	754.5
84	554.0
85	431.0
86	308.0
87	250.5
88	193.0
89	148.5
90	104.0
91	87.0
92	70.0
93	53.0
94	36.0
95	31.5
96	27.0
97	27.5
98	28.0
99	38.5
100	49.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08319458279240596
2	0.09021804505829753
3	0.09843995525051184
4	0.09983349935088716
5	0.09161158915867283
6	0.055686022250997363
7	0.05016758761351113
8	0.05710743723338018
9	0.05794356369360536
10	0.042085031831334344
11	0.04562463384628763
12	0.055769634897019874
13	0.049108494097225903
14	0.04994462055745108
15	0.04969378261938353
16	0.052034936708014054
17	0.051811969651954004
18	0.05242512905611913
19	0.06073065189435598
20	0.0662212156498347
21	0.07681215081268705
22	0.07954349724942265
23	0.05643853606520003
24	0.0734676449717863
25	0.08104852487782799
26	0.07803846962101732
27	0.07369061202784635
28	0.09113778416454524
29	0.08060259076570789
30	0.07684002169469456
31	0.05326125551634432
32	0.049303590271278445
33	0.05236938729210412
34	0.04693456530064042
35	0.053874414920509456
36	0.05289893405024674
37	0.05652214871122254
38	0.04657224383454284
39	0.05105945583775134
40	0.05281532140422422
41	0.03985536127073385
42	0.03726336924403577
43	0.038238850114298485
44	0.03584195426165296
45	0.027870882007506186
46	0.02513953557077058
47	0.029431651399926533
48	0.02491656851471053
49	0.024721472340657986
50	0.025334631744823123
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3587974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.45849384963889
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2525554734073	45.933205944844744
2	12.178869396966748	14.482744622702057
3	4.403966126069394	7.855595784627452
4	2.069697922334642	4.922444847429789
5	1.1037212018398925	3.281280014565665
6	0.6868712888228959	2.450419938118182
7	0.4564101388495194	1.8996221603587888
8	0.3181276560409081	1.5132313024086697
9	0.23982389893974523	1.2833611038094737
>10	1.217051575971374	12.569193412331774
>50	0.05674581846316266	2.270898060203174
>100	0.015875896284455078	1.417883562496101
>500	2.836060100521447E-4	0.12011924610413494
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.361264602251856E-5
2	2.7870882007506188E-5	0.0	0.0	0.0	8.361264602251856E-5
3	2.7870882007506188E-5	0.0	0.0	0.0	8.361264602251856E-5
4	2.7870882007506188E-5	0.0	0.0	0.0	1.1148352803002475E-4
5	5.5741764015012375E-5	0.0	0.0	0.0	1.1148352803002475E-4
6	5.5741764015012375E-5	0.0	0.0	2.7870882007506188E-5	1.3935441003753093E-4
7	1.950961740525433E-4	0.0	0.0	2.7870882007506188E-5	1.6722529204503712E-4
8	1.950961740525433E-4	0.0	0.0	2.7870882007506188E-5	1.950961740525433E-4
9	1.950961740525433E-4	0.0	0.0	2.7870882007506188E-5	1.950961740525433E-4
10	1.950961740525433E-4	0.0	0.0	2.7870882007506188E-5	1.950961740525433E-4
11	1.950961740525433E-4	0.0	0.0	5.5741764015012375E-5	1.950961740525433E-4
12	1.950961740525433E-4	0.0	0.0	5.5741764015012375E-5	2.7870882007506186E-4
13	1.950961740525433E-4	0.0	0.0	8.361264602251856E-5	2.7870882007506186E-4
14	2.5083793806755566E-4	0.0	0.0	1.1148352803002475E-4	2.7870882007506186E-4
15	2.7870882007506186E-4	0.0	0.0	1.1148352803002475E-4	4.180632301125928E-4
16	2.7870882007506186E-4	0.0	0.0	1.1148352803002475E-4	4.45934112120099E-4
17	2.7870882007506186E-4	0.0	0.0	1.1148352803002475E-4	4.45934112120099E-4
18	3.0657970208256805E-4	0.0	0.0	1.1148352803002475E-4	4.7380499412760514E-4
19	3.901923481050866E-4	0.0	0.0	1.1148352803002475E-4	4.7380499412760514E-4
20	3.901923481050866E-4	0.0	0.0	1.3935441003753093E-4	4.7380499412760514E-4
21	3.901923481050866E-4	0.0	0.0	1.3935441003753093E-4	5.295467581426176E-4
22	3.901923481050866E-4	0.0	0.0	2.229670560600495E-4	5.574176401501237E-4
23	3.901923481050866E-4	0.0	0.0	2.7870882007506186E-4	6.410302861726422E-4
24	5.016758761351113E-4	0.0	0.0	4.45934112120099E-4	6.410302861726422E-4
25	5.016758761351113E-4	0.0	0.0	4.7380499412760514E-4	6.689011681801485E-4
26	5.016758761351113E-4	0.0	0.0	7.803846962101732E-4	6.689011681801485E-4
27	5.016758761351113E-4	2.7870882007506188E-5	0.0	0.00117057704431526	6.967720501876546E-4
28	5.574176401501237E-4	2.7870882007506188E-5	0.0	0.002229670560600495	6.967720501876546E-4
29	7.52513814202667E-4	2.7870882007506188E-5	0.0	0.0034002476049157545	6.967720501876546E-4
30	7.803846962101732E-4	2.7870882007506188E-5	0.0	0.0059643687496063235	6.967720501876546E-4
31	7.803846962101732E-4	2.7870882007506188E-5	0.0	0.01387969923973808	7.246429321951609E-4
32	7.803846962101732E-4	2.7870882007506188E-5	0.0	0.022157351195967418	7.246429321951609E-4
33	8.082555782176794E-4	2.7870882007506188E-5	0.0	0.03330570399896989	7.246429321951609E-4
34	8.082555782176794E-4	2.7870882007506188E-5	0.0	0.04657224383454284	7.246429321951609E-4
35	8.639973422326918E-4	2.7870882007506188E-5	0.0	0.06499489684150443	7.246429321951609E-4
36	8.91868224240198E-4	2.7870882007506188E-5	0.0	0.0864833468692917	7.246429321951609E-4
37	8.91868224240198E-4	5.5741764015012375E-5	0.0	0.11388042388267028	7.246429321951609E-4
38	8.91868224240198E-4	5.5741764015012375E-5	0.0	0.14609916348334742	7.52513814202667E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4285	0.0	19.617624	1
GGTATCA	2395	0.0	19.019485	1
TCAACGC	5295	0.0	15.584293	4
TATCAAC	5320	0.0	15.3874	2
GTACATG	9810	0.0	15.320948	1
CAACGCA	5420	0.0	15.222328	5
ATCAACG	5410	0.0	15.212769	3
GTATAGG	575	0.0	14.542855	1
TACATGG	10065	0.0	14.539244	2
AACGCAG	5735	0.0	14.460535	6
GTATTAG	385	0.0	14.289374	1
GTATATG	710	0.0	13.947234	1
ACATGGG	10535	0.0	13.619055	3
GCACCGT	530	0.0	12.451504	6
GTATTAA	655	0.0	12.430664	1
GTATAAC	375	0.0	12.323155	1
GTATAGC	600	0.0	12.1031	1
ACGCAGA	6795	0.0	12.010494	7
CATGGGG	7835	0.0	11.880183	4
TACCGTA	205	6.2182717E-6	11.803621	7
>>END_MODULE