FastQCFastQC Report
Mon 6 Feb 2017
SRR2077123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587974
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA44050.019.8803141
GGTATCA24750.019.0250021
TATCAAC59500.014.681342
GTACATG100500.014.38419151
TCAACGC60300.014.3041134
TACCGTA2002.116758E-814.3014927
ATCAACG61100.014.2608743
CAACGCA62150.013.8783275
TACATGG102800.013.8699242
ACTATAC4650.013.7226263
CGTATAC1150.001423103313.3934483
TATACTG6900.013.3934475
CGTACAC3001.2732926E-1113.2021133
AACGCAG65350.013.197646
ACATGGG105750.013.0876733
TCGCACG1751.5959036E-512.57396713
GCGCGTT1250.002669655512.31836436
GTATAGA5750.012.2452671
TACTATA4400.012.001922
ACCGTAT2758.572897E-912.0014198
Produced by FastQC (version 0.11.5)