FastQCFastQC Report
Mon 6 Feb 2017
SRR2077122_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077122_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298524
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15300.021.8624131
GTATCAA28600.021.2367971
TACCGAT3550.016.7302887
GTACATG84500.016.4330671
TACATGG85050.015.7834342
TATCAAC38700.015.5237862
TCAACGC38200.015.49677854
CAACGCA38100.015.478995
ATCAACG39000.015.2353243
TGCGATA3050.015.14609413
AACGCAG39300.015.1126016
ACATGGG88200.014.8706583
GCGATAC3150.014.66682314
GTACCGA4650.014.6648226
CATGGGG62900.014.3445114
TAGGACT6850.014.1356124
CGAATCG3600.014.0533637
TCGATCG950.006253083713.89130241
TCGGCAC4300.013.81619721
CGGCACG4800.013.75285922
Produced by FastQC (version 0.11.5)