##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077122_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3298524 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.28467065875525 31.0 30.0 34.0 23.0 34.0 2 29.29506652066197 31.0 30.0 34.0 23.0 34.0 3 29.37009068298427 31.0 30.0 34.0 23.0 34.0 4 32.77274775020585 37.0 35.0 37.0 25.0 37.0 5 32.73147777612047 37.0 35.0 37.0 25.0 37.0 6 32.80852041701076 37.0 35.0 37.0 25.0 37.0 7 32.61988301434217 37.0 33.0 37.0 24.0 37.0 8 32.5654371470391 37.0 33.0 37.0 23.0 37.0 9 33.93870470549858 38.0 34.0 39.0 23.0 39.0 10 33.86104785049313 38.0 34.0 39.0 23.0 39.0 11 33.95199731758811 38.0 35.0 39.0 23.0 39.0 12 33.8333554644441 38.0 34.0 39.0 23.0 39.0 13 33.79545366351738 38.0 34.0 39.0 22.0 39.0 14 34.75320870789481 39.0 34.0 41.0 19.0 41.0 15 34.80722317012094 39.0 34.0 41.0 22.0 41.0 16 34.780790135224116 39.0 34.0 41.0 22.0 41.0 17 34.619594400404544 39.0 34.0 41.0 18.0 41.0 18 34.6034032191368 39.0 34.0 41.0 18.0 41.0 19 34.72578371417034 39.0 34.0 41.0 19.0 41.0 20 34.70710960417448 39.0 34.0 41.0 19.0 41.0 21 34.65218018725951 39.0 34.0 41.0 19.0 41.0 22 34.57856362421495 39.0 34.0 41.0 18.0 41.0 23 34.53970351587559 39.0 34.0 41.0 18.0 41.0 24 34.54825279428011 39.0 34.0 41.0 18.0 41.0 25 34.45761134374041 39.0 34.0 41.0 18.0 41.0 26 34.40894654700102 39.0 34.0 41.0 18.0 41.0 27 34.35214962813671 39.0 34.0 41.0 17.0 41.0 28 34.248335012872424 39.0 34.0 41.0 17.0 41.0 29 34.23005562487949 39.0 34.0 41.0 16.0 41.0 30 34.07908840438936 39.0 34.0 41.0 16.0 41.0 31 34.08342155461049 39.0 34.0 41.0 16.0 41.0 32 33.94576907732064 39.0 34.0 41.0 15.0 41.0 33 33.909602901176406 39.0 33.0 41.0 15.0 41.0 34 33.83920383783777 39.0 33.0 41.0 14.0 41.0 35 33.77563995290014 39.0 33.0 41.0 9.0 41.0 36 33.69019052157874 38.0 33.0 41.0 9.0 41.0 37 33.600405211543105 38.0 33.0 41.0 9.0 41.0 38 33.52209988467569 38.0 33.0 40.0 9.0 41.0 39 33.4172723921366 38.0 33.0 40.0 8.0 41.0 40 33.35050374046089 38.0 33.0 40.0 8.0 41.0 41 33.243262744184975 38.0 33.0 40.0 2.0 41.0 42 33.10557813130964 38.0 33.0 40.0 2.0 41.0 43 33.02843089818355 38.0 33.0 40.0 2.0 41.0 44 32.891770682887255 38.0 32.0 40.0 2.0 41.0 45 32.755514284570914 38.0 32.0 40.0 2.0 41.0 46 32.62281099061277 38.0 32.0 40.0 2.0 41.0 47 32.483379535816624 38.0 32.0 40.0 2.0 41.0 48 32.328899228867215 38.0 32.0 40.0 2.0 41.0 49 32.15486532764351 38.0 32.0 40.0 2.0 41.0 50 31.949280647950417 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.6489094602236172 1101 2 1.7566709067824 1101 3 1.6523986389257423 1101 4 1.8976810402699726 1101 5 1.8890171430367317 1101 6 1.8350981714916017 1101 7 1.8392246108479924 1101 8 1.8961779928687292 1101 9 2.0919529469069644 1101 10 2.1248540223507604 1101 11 2.1297133416110157 1101 12 2.22362940903367 1101 13 2.139658634026439 1101 14 2.6719659269154477 1101 15 2.6805285497993694 1101 16 2.7138340931895897 1101 17 2.7465435717273508 1101 18 2.800633735632019 1101 19 2.7097224380583285 1101 20 2.793805076017577 1101 21 2.769776102580252 1101 22 2.779862553262305 1101 23 2.8755139139088826 1101 24 2.841720534126239 1101 25 2.878293225914483 1101 26 2.863240330751843 1101 27 2.9440042002173 1101 28 3.0374669390387012 1101 29 2.9693279387997435 1101 30 3.042347619060486 1101 31 3.0189145539594264 1101 32 3.1243998367434145 1101 33 3.12098460816366 1101 34 3.043951171587885 1101 35 3.05210793770555 1101 36 3.070793107503455 1101 37 3.233549536668967 1101 38 3.294266762397015 1101 39 3.1304170963450204 1101 40 3.0680645893784586 1101 41 3.1144250124758344 1101 42 3.2709520627331585 1101 43 3.1670243154512647 1101 44 3.2704946663760452 1101 45 3.3004089042609834 1101 46 3.129251410239455 1101 47 3.145402524887743 1101 48 3.1468705296235626 1101 49 3.3132901984216616 1101 50 3.3494376200947045 1102 1 0.9161720818443833 1102 2 0.8195682541027836 1102 3 0.9002106536311594 1102 4 0.9331950959857522 1102 5 0.9659652541513637 1102 6 0.9637182710067052 1102 7 1.0354195051528094 1102 8 1.062417138116409 1102 9 1.1509402064385483 1102 10 1.1722815648982206 1102 11 1.098942297653231 1102 12 1.2269521913364585 1102 13 1.1846286051860773 1102 14 1.4274434171699397 1102 15 1.253190420302964 1102 16 1.4083423412167235 1102 17 1.64678126703366 1102 18 1.5241899347256123 1102 19 1.4766778384956822 1102 20 1.4027267712605038 1102 21 1.4237321447949327 1102 22 1.4550340900416927 1102 23 1.3810886611595379 1102 24 1.265129758288758 1102 25 1.4569440277399863 1102 26 1.4090557048842527 1102 27 1.2554809219236347 1102 28 1.474527004642603 1102 29 1.4551137594824013 1102 30 1.4667823003903138 1102 31 1.4798097144829896 1102 32 1.6703501029621535 1102 33 1.5613821139475732 1102 34 1.6060441183758272 1102 35 1.6523507830768267 1102 36 1.5940779377890095 1102 37 1.5813253028401704 1102 38 1.7038985723675424 1102 39 1.6878424996347192 1102 40 1.6052781665343616 1102 41 1.579517277870572 1102 42 1.6711228071948625 1102 43 1.5084967982579727 1102 44 1.353108245116573 1102 45 1.4620900439307505 1102 46 1.5781968353971294 1102 47 1.4319860114024934 1102 48 1.4635048433179776 1102 49 1.3838619348651022 1102 50 1.4791532968924699 1103 1 0.44025907976707046 1103 2 0.5203473184764356 1103 3 0.3863193437715573 1103 4 0.42743674711818613 1103 5 0.5385565903735312 1103 6 0.45380061442311614 1103 7 0.44611287676709566 1103 8 0.612637744570776 1103 9 0.6627979609237897 1103 10 0.6005448513455534 1103 11 0.5154562330807764 1103 12 0.528795608472997 1103 13 0.5851932755963247 1103 14 0.6520219941961827 1103 15 0.6235802117937581 1103 16 0.5285717784118589 1103 17 0.8129432513428867 1103 18 0.5091239238578922 1103 19 0.6272634873174354 1103 20 0.5883985882322378 1103 21 0.4041773841180927 1103 22 0.4506675192214544 1103 23 0.5031070256429615 1103 24 0.4375154880709715 1103 25 0.6399271866673857 1103 26 0.5479419727222066 1103 27 0.5415612686994749 1103 28 0.6045874836922849 1103 29 0.48775004532757293 1103 30 0.7089475389643738 1103 31 0.495286199934597 1103 32 0.614227630095435 1103 33 0.6156582164936566 1103 34 0.5501586205266094 1103 35 0.5925965240091173 1103 36 0.7364719220809519 1103 37 0.7235775703093381 1103 38 0.6095250723689816 1103 39 0.8212060430810979 1103 40 0.7415928232591185 1103 41 0.7528310997806003 1103 42 0.730343332256588 1103 43 0.6413134624276395 1103 44 0.5168703171569788 1103 45 0.5499483515977914 1103 46 0.4723831683491895 1103 47 0.5412375268901428 1103 48 0.4970106978254023 1103 49 0.6452885964993484 1103 50 0.6629338155292217 1104 1 0.8224936292486973 1104 2 0.7964847067453888 1104 3 0.7708561380109664 1104 4 0.7485941674824375 1104 5 0.6964603276877739 1104 6 0.6682936477281558 1104 7 0.8136985412372013 1104 8 0.8663892826720101 1104 9 0.9807122414865148 1104 10 0.7550895991153865 1104 11 0.826049931515449 1104 12 0.8731974577360404 1104 13 0.8738186592429642 1104 14 1.0437082079461817 1104 15 1.043889378496857 1104 16 0.9833688297132426 1104 17 1.0884289463964052 1104 18 0.9161947990865684 1104 19 0.9836883196944584 1104 20 0.9802859257401053 1104 21 1.0070757710640734 1104 22 1.0164352185154684 1104 23 0.9956065998490615 1104 24 1.0161168882088418 1104 25 1.0848201988920678 1104 26 1.0698798654292148 1104 27 1.0938327802413141 1104 28 0.9570950121970441 1104 29 1.0730114612711006 1104 30 1.0870249802153822 1104 31 1.1373194059433303 1104 32 1.1544807679295062 1104 33 1.2218326236721566 1104 34 1.0819196188195193 1104 35 1.1520058547225602 1104 36 1.1727701202410117 1104 37 1.2630803352232434 1104 38 1.2109331614620444 1104 39 1.177981766506278 1104 40 1.1428212761096148 1104 41 1.1827355137556737 1104 42 1.2060751158256053 1104 43 1.2026079250480777 1104 44 1.2172434332254412 1104 45 1.2060521157848711 1104 46 1.092566521309628 1104 47 1.150119384044494 1104 48 1.0922243595522971 1104 49 1.2338133568083052 1104 50 1.2573989096867138 1105 1 0.4396052120158487 1105 2 0.4593889903900461 1105 3 0.4585476492969782 1105 4 0.4957586414726052 1105 5 0.4380428678168471 1105 6 0.42797092097405454 1105 7 0.5190735049709545 1105 8 0.553583884918126 1105 9 0.5622875360815343 1105 10 0.4497937470330129 1105 11 0.5307904518376532 1105 12 0.5175494052543357 1105 13 0.5622894217645253 1105 14 0.615103314147035 1105 15 0.5717196643148768 1105 16 0.5138320685463071 1105 17 0.495474813154253 1105 18 0.44421570078453954 1105 19 0.43190780427411823 1105 20 0.5520771779516167 1105 21 0.6447798566063341 1105 22 0.5575398487438079 1105 23 0.5678290428870909 1105 24 0.604952337222926 1105 25 0.4815935631863084 1105 26 0.629749525163156 1105 27 0.6449603380077704 1105 28 0.6909325378185116 1105 29 0.6307004444813984 1105 30 0.712160356428825 1105 31 0.4624767788399353 1105 32 0.5076076140731445 1105 33 0.6490313166842014 1105 34 0.6148069376875966 1105 35 0.5881796573194507 1105 36 0.677404411424348 1105 37 0.6847836411101937 1105 38 0.7127203736013925 1105 39 0.7349830043890933 1105 40 0.6886815319308965 1105 41 0.6616913222428806 1105 42 0.7549238054617362 1105 43 0.7360735935815725 1105 44 0.7042005903135689 1105 45 0.8296381731791698 1105 46 0.8594395164724915 1105 47 0.6875250596736464 1105 48 0.7935210103919772 1105 49 0.7867513429862498 1105 50 0.8225297734209391 1106 1 0.3715720637030806 1106 2 0.28618284350013923 1106 3 0.2661438949944106 1106 4 0.3171757172847194 1106 5 0.2847166418255682 1106 6 0.2142625100603226 1106 7 0.2531305263997936 1106 8 0.36297171703512987 1106 9 0.47089760145591697 1106 10 0.49498528562506294 1106 11 0.404891036968138 1106 12 0.4426867076000036 1106 13 0.49512561593276416 1106 14 0.5823346810500638 1106 15 0.5361175599612054 1106 16 0.4386020156751229 1106 17 0.596686905187795 1106 18 0.2244965599561013 1106 19 0.42026710520801913 1106 20 0.2970442961641737 1106 21 0.41532994671899814 1106 22 0.3901124293273597 1106 23 0.37447385514962406 1106 24 0.47632201073444946 1106 25 0.49413733481807043 1106 26 0.44828440848517204 1106 27 0.5356384669452012 1106 28 0.5002735225957267 1106 29 0.4653595335067706 1106 30 0.5867353970022222 1106 31 0.5044659774289784 1106 32 0.5138850134605519 1106 33 0.41221001391589596 1106 34 0.5339435282419913 1106 35 0.5188912165185613 1106 36 0.5747737819416869 1106 37 0.49689878120464215 1106 38 0.5827730602819159 1106 39 0.5752072690912655 1106 40 0.6996670889681056 1106 41 0.6291209615025082 1106 42 0.6686451318266862 1106 43 0.5128477491871024 1106 44 0.512185885149826 1106 45 0.5511574050211792 1106 46 0.5176844248513461 1106 47 0.5351730421316958 1106 48 0.595011173820005 1106 49 0.6300969378299897 1106 50 0.6090371485616046 1107 1 0.25632821780518 1107 2 0.19286693956912515 1107 3 0.34699753850453163 1107 4 0.28526160331499995 1107 5 0.2595087638252309 1107 6 0.2514900602642598 1107 7 0.30308914870218473 1107 8 0.3574831755874328 1107 9 0.3561750152470182 1107 10 0.36165707118215096 1107 11 0.3100128225042482 1107 12 0.20131158342145739 1107 13 0.30640960847708953 1107 14 0.12481119184651845 1107 15 0.30460881735789513 1107 16 0.3386757500749198 1107 17 0.3043194505336828 1107 18 0.36425107978949 1107 19 0.3296721153150344 1107 20 0.35385775610473047 1107 21 0.2785843834053878 1107 22 0.029391493750829056 1107 23 0.04690116166080571 1107 24 0.07546869449765126 1107 25 0.19229028250874336 1107 26 0.33062664211440307 1107 27 0.2909505395405745 1107 28 0.2386885015320459 1107 29 0.36632592687223564 1107 30 0.317468473859563 1107 31 0.30494057969049493 1107 32 0.35904738928025637 1107 33 0.42645577408300994 1107 34 0.34094457593619865 1107 35 0.3936184712674162 1107 36 0.29517082602022526 1107 37 0.37466128622737216 1107 38 0.23397802051471217 1107 39 0.4096721429434851 1107 40 0.3909426115669632 1107 41 0.4802317397458822 1107 42 0.35203175896321426 1107 43 0.28977202890957443 1107 44 0.41705466323291773 1107 45 0.5317864607103715 1107 46 0.435979171401236 1107 47 0.39924573205406944 1107 48 0.38678478124055005 1107 49 0.42322509316789336 1107 50 0.550374664280163 1108 1 0.08296176436274294 1108 2 0.08160880836587125 1108 3 0.10491317242269815 1108 4 0.07321623814596023 1108 5 0.05366710536642927 1108 6 -0.03351605505000066 1108 7 0.020754650031683752 1108 8 0.11937286181228757 1108 9 0.03313579425507385 1108 10 0.04497308681138179 1108 11 0.06453977859305127 1108 12 0.008875812260484395 1108 13 -0.00839468342724814 1108 14 -0.08043066071487459 1108 15 -0.03168205512968569 1108 16 -0.11089094653694787 1108 17 -0.11390129968368967 1108 18 -0.046308390288764656 1108 19 -0.047406460159493236 1108 20 -0.08093657811973998 1108 21 -0.011553905677608611 1108 22 -0.13832956854600553 1108 23 -0.38658532511820454 1108 24 -0.28166926627053357 1108 25 -0.06830147787195529 1108 26 -0.2126396572726179 1108 27 -0.12099795472112618 1108 28 -0.26510155016851655 1108 29 -0.31712177546528864 1108 30 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46 -0.9410077139122812 2214 47 -0.8114317407940064 2214 48 -0.8438473706670884 2214 49 -0.8660115069690271 2214 50 -0.8272114861450817 2215 1 -0.591362631461255 2215 2 -0.7035461407737778 2215 3 -0.688420461770054 2215 4 -0.6800055737737836 2215 5 -0.6658341396367433 2215 6 -0.7937138698904747 2215 7 -0.6559673292100996 2215 8 -0.6378637994817637 2215 9 -0.7055478772383736 2215 10 -0.7650531705024477 2215 11 -0.876745882550928 2215 12 -0.8683500005031064 2215 13 -0.839062851143332 2215 14 -0.8589809901424061 2215 15 -0.9520860542955774 2215 16 -0.9318898895596277 2215 17 -0.683598676158006 2215 18 -0.7697890648976653 2215 19 -0.9011688920923007 2215 20 -0.97760622000186 2215 21 -0.8731407573182324 2215 22 -0.8387840444342416 2215 23 -0.9822969485262973 2215 24 -1.053903713067875 2215 25 -0.9756405215801536 2215 26 -1.0772956470836377 2215 27 -0.983609577736793 2215 28 -0.8935328191055731 2215 29 -1.0741957155684645 2215 30 -1.051490008741908 2215 31 -0.9976613436406154 2215 32 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-1.487787461379483 2216 19 -1.5439728387647023 2216 20 -1.6552493203936507 2216 21 -1.785895098465403 2216 22 -1.5817912848232538 2216 23 -1.720084027063514 2216 24 -1.8740747572197165 2216 25 -1.9151843684441658 2216 26 -1.8045010321437047 2216 27 -1.7071834520716749 2216 28 -1.7997916173828514 2216 29 -2.0059016040509476 2216 30 -1.8746587226154574 2216 31 -1.9262533471556083 2216 32 -1.944989877800488 2216 33 -1.9388837173434368 2216 34 -2.011157669644305 2216 35 -1.9991378062714702 2216 36 -2.0547946674235256 2216 37 -2.165180514483989 2216 38 -2.1296202169965674 2216 39 -2.0450924161689024 2216 40 -2.0394921710417364 2216 41 -2.1174987905933413 2216 42 -1.9956403756654453 2216 43 -2.3464731269008006 2216 44 -2.45526576995708 2216 45 -2.3584879991293093 2216 46 -2.485051247455271 2216 47 -2.428999211408268 2216 48 -2.5260038793741977 2216 49 -2.58048834315424 2216 50 -2.68110327806734 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 166275.0 3 8279.0 4 15217.0 5 11450.0 6 9404.0 7 8052.0 8 7275.0 9 6583.0 10 6545.0 11 6520.0 12 6739.0 13 7174.0 14 7554.0 15 8157.0 16 8694.0 17 9415.0 18 10241.0 19 11480.0 20 13795.0 21 17540.0 22 20921.0 23 23736.0 24 26167.0 25 29563.0 26 32968.0 27 37246.0 28 42794.0 29 49521.0 30 58703.0 31 70040.0 32 85983.0 33 107372.0 34 137721.0 35 180797.0 36 240941.0 37 334065.0 38 527047.0 39 946550.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.816048685195085 16.288730928486338 11.073630410283704 25.821589976034875 2 17.221522404174912 18.745965846519027 37.032853751447895 26.99965799785816 3 20.23382119170327 22.399897787065694 27.870656936610377 29.49562408462066 4 13.447851189305595 14.27303285673705 34.98054040142857 37.29857555252878 5 14.983722053764565 36.585746297101046 33.46708116152433 14.963450487610066 6 35.92052686035962 33.90664068886671 16.021544223079047 14.151288227694629 7 31.13538313982745 30.391540400248847 19.764595900447677 18.708480559476026 8 28.489867483274256 33.83385433018915 18.664003288271278 19.012274898265318 9 27.320058133979348 14.6500722124493 17.525101447798182 40.504768205773175 10 14.946797314175797 28.525386910701624 32.99482501439181 23.532990760730772 11 38.05587596652817 21.367002114965175 21.338703498746277 19.238418419760382 12 23.878804608300523 23.486104117500744 29.195397766223998 23.439693507974738 13 29.62161188260441 20.11433327479266 24.659091563985413 25.60496327861752 14 22.674196755780876 19.53793021028155 25.449448528854184 32.33842450508339 15 24.89347362137516 27.885979163052088 22.053447608555597 25.16709960701715 16 25.210169934696037 26.660391775261417 23.76759201598429 24.36184627405826 17 24.07746970398769 26.750428683531304 25.067150056070865 24.104951556410143 18 24.120928604427046 26.390751447892114 25.452780046889472 24.03553990079137 19 24.96506035828794 26.048727717730774 25.292963624852455 23.69324829912884 20 24.865945052868085 25.605427788107782 25.55743346248115 23.971193696542983 21 25.092820909121528 25.36677040277463 25.417500666744946 24.122908021358896 22 24.88763773915571 25.091836217649483 25.37015904725177 24.650366995943035 23 24.711361793556456 25.166466915468387 25.43422755741444 24.68794373356072 24 24.37566653670939 25.30577632279567 25.34588609371184 24.972671046783102 25 24.642653308853312 25.121633959382223 25.505249337859166 24.7304633939053 26 24.43042867736388 25.58559165389288 25.60061071444609 24.38336895429715 27 24.554412008318007 25.574199130941857 25.350896940577954 24.520491920162186 28 24.736521396617157 25.52114595971152 25.26059573930897 24.48173690436235 29 24.682152026938873 25.596630577851894 25.229340261026444 24.491877134182786 30 24.604836896514374 25.717559153477875 25.34132170395513 24.33628224605262 31 24.667257965394466 25.22954786880691 25.257666913984846 24.845527251813785 32 24.466184803567835 25.678911012449397 25.255074107001757 24.59983007698101 33 24.669657909919334 25.35940115277726 25.236855627417924 24.734085309885483 34 24.850903858391575 25.38544181049518 25.20305647848593 24.560597852627314 35 24.679831451761395 25.468668998547905 25.38840504358551 24.463094506105186 36 24.686158961831893 25.547578104216612 25.21548370422971 24.55077922972178 37 24.72184522830866 25.147381532064166 25.392395555833286 24.738377683793885 38 24.77808651964137 25.428911402341537 25.408376917297588 24.3846251607195 39 24.926911715904822 25.533488357137962 25.086980973082763 24.452618953874453 40 24.803999662690746 25.2829363377315 25.5144117805864 24.39865221899136 41 24.565523591841487 25.41181994205768 25.447184653535015 24.575471812565816 42 24.7927196983161 25.268301293268948 25.421369357150525 24.517609651264426 43 24.577254541351962 25.22694628503216 25.409370700970747 24.786428472645134 44 24.713425749997043 25.393252020660462 25.265208368147036 24.628113861195462 45 24.640335711939844 25.41505324041292 25.18776597083506 24.75684507681218 46 24.685755492285313 25.296732148234312 25.14883989336717 24.868672466113207 47 24.500816519005863 25.39481764408869 25.285766489210882 24.818599347694565 48 24.426735773543573 25.49866664402458 25.438078851464084 24.63651873096776 49 24.71371600761382 25.266709666502614 25.27871808331112 24.740856242572445 50 24.754370685566577 25.31598240202423 25.378451091867788 24.551195820541402 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1084.0 1 745.0 2 406.0 3 437.0 4 468.0 5 490.0 6 512.0 7 526.5 8 541.0 9 612.0 10 683.0 11 847.5 12 1012.0 13 1404.0 14 1796.0 15 2380.0 16 2964.0 17 3480.5 18 3997.0 19 4429.0 20 4861.0 21 5962.5 22 7064.0 23 9475.0 24 11886.0 25 15952.0 26 20018.0 27 25279.0 28 30540.0 29 37247.5 30 43955.0 31 52094.0 32 60233.0 33 70141.0 34 80049.0 35 90727.0 36 101405.0 37 113119.0 38 124833.0 39 136441.5 40 148050.0 41 160907.0 42 173764.0 43 184266.0 44 194768.0 45 206905.0 46 219042.0 47 230002.5 48 240963.0 49 247273.5 50 253584.0 51 257802.0 52 262020.0 53 258605.5 54 255191.0 55 248254.0 56 241317.0 57 228701.0 58 216085.0 59 197367.5 60 178650.0 61 158453.5 62 138257.0 63 118864.5 64 99472.0 65 83832.0 66 68192.0 67 56565.5 68 44939.0 69 36317.0 70 27695.0 71 21944.5 72 16194.0 73 13166.0 74 10138.0 75 7822.0 76 5506.0 77 4227.0 78 2948.0 79 2269.0 80 1590.0 81 1214.5 82 839.0 83 643.0 84 447.0 85 342.0 86 237.0 87 187.5 88 138.0 89 103.0 90 68.0 91 53.0 92 38.0 93 28.5 94 19.0 95 16.0 96 13.0 97 11.0 98 9.0 99 26.5 100 44.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08797874443235823 2 0.09771036985027241 3 0.10434970307931668 4 0.10586553258366468 5 0.09907461640418563 6 0.05647980733200667 7 0.05229611790000618 8 0.05939019998035485 9 0.06066349676400718 10 0.04577805103130976 11 0.04677849850417944 12 0.05651012392209364 13 0.05065902203531034 14 0.05172010268835395 15 0.051174404066788665 16 0.054175746485397705 17 0.05466081192678908 18 0.0556309428095718 19 0.06442275393479023 20 0.0697584737900952 21 0.08067244622140085 22 0.08218827572574885 23 0.05829880273722428 24 0.07788331993340052 25 0.0841285374913143 26 0.08221859231583581 27 0.07706477200105259 28 0.09670992237740274 29 0.08385568818053166 30 0.08285524070766197 31 0.05535809349878916 32 0.0526599169810497 33 0.05426669625565859 34 0.050264906364179865 35 0.057631837755311166 36 0.05672234005270236 37 0.05996621519200709 38 0.049446358431831934 39 0.05535809349878916 40 0.05596442530052836 41 0.044019688806266076 42 0.03935093393287422 43 0.03844143623026541 44 0.03671339059530869 45 0.0283460117313077 46 0.025860051344176974 47 0.029983107596003544 48 0.02516276977217689 49 0.025374985902785613 50 0.026436066555829214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3298524.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.44885474190961 #Duplication Level Percentage of deduplicated Percentage of total 1 78.74759599830969 47.602019917764046 2 12.029637097384152 14.543555709953235 3 3.8593362162860254 6.998773630153951 4 1.7367939223454618 4.19948814113969 5 0.956386566820541 2.890623632742426 6 0.596335003699895 2.162866080970265 7 0.4072428804020851 1.7232156005471757 8 0.2696826079940683 1.3041603837642226 9 0.20557472131358684 1.1184080820564206 >10 1.1004458620021675 11.786051655792248 >50 0.06211399474141594 2.525457206247106 >100 0.027947441439221 2.7367304067131006 >500 8.572601917710532E-4 0.3775573141982338 >1k 5.042706989799121E-5 0.03109223795790269 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.031659008696011E-5 0.0 0.0 0.0 0.0 6 3.031659008696011E-5 0.0 0.0 0.0 3.031659008696011E-5 7 3.031659008696011E-5 0.0 0.0 0.0 3.031659008696011E-5 8 3.031659008696011E-5 0.0 0.0 0.0 3.031659008696011E-5 9 3.031659008696011E-5 0.0 0.0 0.0 3.031659008696011E-5 10 3.031659008696011E-5 0.0 0.0 0.0 3.031659008696011E-5 11 3.031659008696011E-5 0.0 0.0 0.0 3.031659008696011E-5 12 3.031659008696011E-5 0.0 0.0 3.031659008696011E-5 3.031659008696011E-5 13 3.031659008696011E-5 0.0 0.0 6.063318017392022E-5 3.031659008696011E-5 14 3.031659008696011E-5 6.063318017392022E-5 0.0 6.063318017392022E-5 3.031659008696011E-5 15 3.031659008696011E-5 9.094977026088032E-5 0.0 6.063318017392022E-5 3.031659008696011E-5 16 3.031659008696011E-5 9.094977026088032E-5 0.0 9.094977026088032E-5 6.063318017392022E-5 17 3.031659008696011E-5 9.094977026088032E-5 0.0 9.094977026088032E-5 6.063318017392022E-5 18 3.031659008696011E-5 9.094977026088032E-5 0.0 9.094977026088032E-5 6.063318017392022E-5 19 3.031659008696011E-5 1.2126636034784043E-4 0.0 9.094977026088032E-5 6.063318017392022E-5 20 3.031659008696011E-5 1.2126636034784043E-4 0.0 9.094977026088032E-5 9.094977026088032E-5 21 3.031659008696011E-5 1.2126636034784043E-4 0.0 9.094977026088032E-5 9.094977026088032E-5 22 3.031659008696011E-5 1.2126636034784043E-4 0.0 1.5158295043480054E-4 9.094977026088032E-5 23 3.031659008696011E-5 1.2126636034784043E-4 0.0 1.8189954052176064E-4 9.094977026088032E-5 24 6.063318017392022E-5 1.5158295043480054E-4 0.0 2.1221613060872074E-4 9.094977026088032E-5 25 6.063318017392022E-5 1.5158295043480054E-4 0.0 2.1221613060872074E-4 9.094977026088032E-5 26 6.063318017392022E-5 1.5158295043480054E-4 0.0 2.7284931078264097E-4 9.094977026088032E-5 27 6.063318017392022E-5 1.5158295043480054E-4 0.0 5.76015211652242E-4 9.094977026088032E-5 28 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.001697729044869766 9.094977026088032E-5 29 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.0038198903509569734 9.094977026088032E-5 30 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.00809452955321835 9.094977026088032E-5 31 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.014461013471479971 9.094977026088032E-5 32 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.0226464927949592 9.094977026088032E-5 33 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.03243875139304731 9.094977026088032E-5 34 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.046141850112353285 9.094977026088032E-5 35 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.06233090921878998 9.094977026088032E-5 36 6.063318017392022E-5 1.5158295043480054E-4 0.0 0.08367378864000989 9.094977026088032E-5 37 6.063318017392022E-5 1.8189954052176064E-4 0.0 0.11362657964592648 9.094977026088032E-5 38 6.063318017392022E-5 1.8189954052176064E-4 0.0 0.14627754716958252 9.094977026088032E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1530 0.0 21.862413 1 GTATCAA 2860 0.0 21.236797 1 TACCGAT 355 0.0 16.730288 7 GTACATG 8450 0.0 16.433067 1 TACATGG 8505 0.0 15.783434 2 TATCAAC 3870 0.0 15.523786 2 TCAACGC 3820 0.0 15.4967785 4 CAACGCA 3810 0.0 15.47899 5 ATCAACG 3900 0.0 15.235324 3 TGCGATA 305 0.0 15.146094 13 AACGCAG 3930 0.0 15.112601 6 ACATGGG 8820 0.0 14.870658 3 GCGATAC 315 0.0 14.666823 14 GTACCGA 465 0.0 14.664822 6 CATGGGG 6290 0.0 14.344511 4 TAGGACT 685 0.0 14.135612 4 CGAATCG 360 0.0 14.05336 37 TCGATCG 95 0.0062530837 13.891302 41 TCGGCAC 430 0.0 13.816197 21 CGGCACG 480 0.0 13.752859 22 >>END_MODULE