FastQCFastQC Report
Mon 6 Feb 2017
SRR2077122_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077122_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3298524
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17100.024.5757771
GTATCAA31650.020.7858331
CGAATCG3250.019.62665737
GTACCGA4200.018.335056
TACCGAT3750.018.1883727
CGTGCCG2950.017.15571210
GCACCGT5000.016.2815276
ATACCGT2201.8189894E-1116.0015016
TCAACGC41250.015.8429254
TATCAAC41300.015.8230272
ATCAACG42150.015.6607823
CAACGCA42700.015.3051675
CGGCACG4700.014.97967322
GTACATG76300.014.9662441
ATTATAC6350.014.8999383
CCAGTAC9950.014.8163593
TATACTG7600.014.7661095
CGAACTA900.00435621914.66915315
GCGATAC2305.9480953E-1014.3502614
CGTACAC3850.014.2879213
Produced by FastQC (version 0.11.5)