FastQCFastQC Report
Mon 6 Feb 2017
SRR2077121_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077121_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008078
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG44020.14633929040403873No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC43920.14600685221593324No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC39360.13084767083832266No Hit
CTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG31580.10498397980371518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54500.017.4849911
TATAGGC12600.017.2897245
TTATAGG12600.016.9415654
ATAGGCC13050.016.518586
CTTATAG13100.016.2957533
TAACGGG850.002980349115.5301730
CTAGTAC1857.545168E-915.4648473
TATCATA8050.015.0360432
TCATACT10000.014.9644654
GTACATG101800.014.8519321
TAGGCCT14550.014.8156347
TACATGG103000.014.5290912
TATACAG4450.014.3404025
GTATTAA3400.014.2402551
TCGCACG1554.5584547E-614.19754922
TACTAGT1402.7507125E-514.147552
AGAATAT17600.014.12827625
ACATGGG104700.014.1042443
AATATCG16700.014.09344833
CGGTAAG1251.6709241E-414.0813916
Produced by FastQC (version 0.11.5)