##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077121_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3008078 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.371792220813422 31.0 30.0 34.0 24.0 34.0 2 29.3997808567464 31.0 30.0 34.0 23.0 34.0 3 29.511859067484288 31.0 30.0 34.0 23.0 34.0 4 32.92451026868319 37.0 35.0 37.0 26.0 37.0 5 32.89045264118816 37.0 35.0 37.0 26.0 37.0 6 32.95283466718616 37.0 35.0 37.0 26.0 37.0 7 32.827123498792254 37.0 35.0 37.0 25.0 37.0 8 32.74176533986153 37.0 35.0 37.0 25.0 37.0 9 34.128407906975816 39.0 35.0 39.0 25.0 39.0 10 34.07802224543379 39.0 35.0 39.0 25.0 39.0 11 34.105173137132745 38.0 35.0 39.0 25.0 39.0 12 34.01773325026811 38.0 35.0 39.0 25.0 39.0 13 33.95381236789738 38.0 35.0 39.0 23.0 39.0 14 34.93034987789545 39.0 34.0 41.0 22.0 41.0 15 34.9663103815792 39.0 35.0 41.0 23.0 41.0 16 34.94717789897735 39.0 35.0 41.0 23.0 41.0 17 34.79174044024124 39.0 34.0 41.0 22.0 41.0 18 34.75607381191578 39.0 34.0 41.0 21.0 41.0 19 34.87044418396066 39.0 34.0 41.0 22.0 41.0 20 34.850250891100565 39.0 34.0 41.0 22.0 41.0 21 34.79810363959977 39.0 34.0 41.0 22.0 41.0 22 34.73589614365053 39.0 34.0 41.0 21.0 41.0 23 34.69906232484663 39.0 34.0 41.0 21.0 41.0 24 34.68273096641776 39.0 34.0 41.0 21.0 41.0 25 34.59352117863965 39.0 34.0 41.0 19.0 41.0 26 34.54641734689061 39.0 34.0 41.0 19.0 41.0 27 34.485717125686236 39.0 34.0 41.0 18.0 41.0 28 34.39310017891823 39.0 34.0 41.0 18.0 41.0 29 34.38518914735589 39.0 34.0 41.0 18.0 41.0 30 34.226259425453726 39.0 34.0 41.0 17.0 41.0 31 34.225760435733385 39.0 34.0 41.0 17.0 41.0 32 34.10485466134854 39.0 34.0 41.0 16.0 41.0 33 34.05961414564383 39.0 34.0 41.0 16.0 41.0 34 33.98593354294669 39.0 33.0 41.0 16.0 41.0 35 33.91444470522374 39.0 33.0 41.0 15.0 41.0 36 33.83772960674557 39.0 33.0 41.0 15.0 41.0 37 33.748999859711084 38.0 33.0 41.0 13.0 41.0 38 33.6649096200298 38.0 33.0 41.0 9.0 41.0 39 33.56355087866738 38.0 33.0 40.0 9.0 41.0 40 33.50604139919244 38.0 33.0 40.0 9.0 41.0 41 33.40018277451582 38.0 33.0 40.0 8.0 41.0 42 33.265440257865656 38.0 33.0 40.0 7.0 41.0 43 33.181489309785185 38.0 33.0 40.0 2.0 41.0 44 33.045315314297035 38.0 33.0 40.0 2.0 41.0 45 32.91229050576481 38.0 32.0 40.0 2.0 41.0 46 32.78568574352128 38.0 32.0 40.0 2.0 41.0 47 32.64997882368742 38.0 32.0 40.0 2.0 41.0 48 32.46617707386577 38.0 32.0 40.0 2.0 41.0 49 32.30840456929641 38.0 32.0 40.0 2.0 41.0 50 32.10267519658732 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8004638282402325 1101 2 0.9251181227222567 1101 3 0.8971633446060814 1101 4 1.0591312969525006 1101 5 1.0987496938823824 1101 6 0.9486075497160513 1101 7 1.0047851308400126 1101 8 1.0845992753063811 1101 9 1.262612808583306 1101 10 1.167046048030464 1101 11 1.1193297024262918 1101 12 1.3265992720981998 1101 13 1.2595947115824728 1101 14 1.5400980787876577 1101 15 1.5517524680768773 1101 16 1.4275381589898473 1101 17 1.6975598234645233 1101 18 1.6922420957689965 1101 19 1.6456224569742588 1101 20 1.5181735370156204 1101 21 1.5889178774436843 1101 22 1.6179443006852523 1101 23 1.6617436976443543 1101 24 1.62284876356118 1101 25 1.6740434988179587 1101 26 1.6962860403198192 1101 27 1.5704708583335574 1101 28 1.6544238389515797 1101 29 1.6510263154689326 1101 30 1.8884701809444806 1101 31 1.8739127787827243 1101 32 1.7989699757350266 1101 33 1.8700031642503632 1101 34 1.9126175902854072 1101 35 1.8727424847672225 1101 36 1.8569411405798988 1101 37 1.890417891736675 1101 38 1.87899109605722 1101 39 2.04318710927528 1101 40 2.0614548762336398 1101 41 1.9743087835541857 1101 42 2.00065207500419 1101 43 1.9698583449175118 1101 44 2.0107150396833404 1101 45 1.894913476787572 1101 46 1.9272518499256748 1101 47 1.909830694881741 1101 48 1.9354331129347884 1101 49 1.9376568493122974 1101 50 1.953926672248059 1102 1 0.513084475349654 1102 2 0.47881815170198294 1102 3 0.5190680082224262 1102 4 0.6485505644611607 1102 5 0.6601035492224128 1102 6 0.6173704471909431 1102 7 0.6882592700062773 1102 8 0.6609099397990335 1102 9 0.794165923719433 1102 10 0.8988541294574972 1102 11 0.7035676347206277 1102 12 0.7845079495676899 1102 13 0.8063161095385993 1102 14 1.0689637736583393 1102 15 0.9137900882747729 1102 16 0.9131952684903624 1102 17 1.1560900215817114 1102 18 1.1091089866868273 1102 19 1.013160926522275 1102 20 0.9617812501375411 1102 21 1.0592069789938918 1102 22 1.017783875563282 1102 23 0.9769878161193688 1102 24 0.8916218891742389 1102 25 1.0618991051507436 1102 26 1.0871822141207659 1102 27 0.8998906234273178 1102 28 1.1834864945909018 1102 29 1.0339892320179231 1102 30 1.0484004821723687 1102 31 1.0874426705165021 1102 32 1.1763509549917188 1102 33 1.136437409531247 1102 34 1.21962045554627 1102 35 1.1931619490062886 1102 36 1.155273815841582 1102 37 1.096839918539004 1102 38 1.1000932654821796 1102 39 1.287203533269384 1102 40 1.2475065522891526 1102 41 1.1555541561279412 1102 42 1.128306610883996 1102 43 1.0422470712977656 1102 44 0.9055214030596019 1102 45 1.0207333979756257 1102 46 0.9139946624701665 1102 47 0.8807977561303204 1102 48 0.9228147233873898 1102 49 0.9775405986897425 1102 50 1.0738686390821641 1103 1 0.39041600652520003 1103 2 0.4553925421970355 1103 3 0.4698064589806563 1103 4 0.4522398253532245 1103 5 0.5049127320228308 1103 6 0.48967254749450007 1103 7 0.5849215805746013 1103 8 0.6339439190404548 1103 9 0.6993264979876983 1103 10 0.7049424187247553 1103 11 0.7068454870999616 1103 12 0.7215756535494577 1103 13 0.7016488822440294 1103 14 0.8461000547949524 1103 15 0.7330189835224701 1103 16 0.7106815890578986 1103 17 0.8271519942663801 1103 18 0.700299587058197 1103 19 0.769273451993314 1103 20 0.6864220813179358 1103 21 0.4954039167767519 1103 22 0.5629449437645491 1103 23 0.5957739925254444 1103 24 0.472017437414749 1103 25 0.5785052999076896 1103 26 0.6489831733553828 1103 27 0.5315134067983465 1103 28 0.49778872214499614 1103 29 0.6167384965600604 1103 30 0.6250868238367033 1103 31 0.6034687032969046 1103 32 0.7837903812257565 1103 33 0.6616469333965824 1103 34 0.6433822115701631 1103 35 0.6374193081893438 1103 36 0.6908381426611356 1103 37 0.6864989080358086 1103 38 0.6476009340012325 1103 39 0.8299800143567708 1103 40 0.7381584517545363 1103 41 0.6961258333402185 1103 42 0.8435258794613176 1103 43 0.827907359252329 1103 44 0.8006254178139613 1103 45 0.7318417208303245 1103 46 0.6063479727421281 1103 47 0.5444286533972758 1103 48 0.611117992386653 1103 49 0.5757581284554831 1103 50 0.6672682292014969 1104 1 0.4999258736503016 1104 2 0.5323718511657098 1104 3 0.5359615419443244 1104 4 0.5007721640435108 1104 5 0.5071601443647964 1104 6 0.5039305688506772 1104 7 0.5863139017665304 1104 8 0.6302261592385605 1104 9 0.6732941848798006 1104 10 0.5937879241558974 1104 11 0.6365108823221917 1104 12 0.6934868366323386 1104 13 0.618302437939434 1104 14 0.7855833290329812 1104 15 0.7734570562480698 1104 16 0.8153835670877498 1104 17 0.8768214698786991 1104 18 0.7506645837069001 1104 19 0.828366518144243 1104 20 0.7485971850544999 1104 21 0.8302808271412019 1104 22 0.9059928672425244 1104 23 0.8300385885540393 1104 24 0.9028554651153513 1104 25 0.9124167950956377 1104 26 0.8850551647392351 1104 27 0.9327152031932826 1104 28 0.7895094315956399 1104 29 0.8605808169845233 1104 30 0.888893955693419 1104 31 0.7824865444507765 1104 32 0.914609094227238 1104 33 0.8616100308091816 1104 34 0.8792879174648505 1104 35 0.831446972978263 1104 36 0.9511711869135411 1104 37 1.0116378843311722 1104 38 1.0229977807167145 1104 39 0.9193181725275323 1104 40 0.9239153321364242 1104 41 0.9622628807239053 1104 42 0.8952940698147103 1104 43 1.0036800751292674 1104 44 0.985446942319328 1104 45 0.9493671241365931 1104 46 1.034341559238726 1104 47 0.9855990857907173 1104 48 1.0091283865088414 1104 49 1.009133288984934 1104 50 1.1544073257340628 1105 1 0.3645701248977886 1105 2 0.3611673301279801 1105 3 0.2559593701087479 1105 4 0.4114090259930592 1105 5 0.2960938791150909 1105 6 0.27149004358762596 1105 7 0.29065978645527935 1105 8 0.4015166576397817 1105 9 0.33919338979545444 1105 10 0.37403746611195743 1105 11 0.39365326481961205 1105 12 0.2579702806667399 1105 13 0.2803785291038281 1105 14 0.40936356233940074 1105 15 0.2768334487945765 1105 16 0.2801957274861451 1105 17 0.3433772203346095 1105 18 0.19477963013144262 1105 19 0.2292093376215405 1105 20 0.4126018892787542 1105 21 0.46734492241841963 1105 22 0.4607927458574963 1105 23 0.37598278098541016 1105 24 0.462899554797076 1105 25 0.39675329599970866 1105 26 0.41883202096553873 1105 27 0.48584197881636015 1105 28 0.4500669678717486 1105 29 0.4351951908542162 1105 30 0.33850481962663537 1105 31 0.2927485784537538 1105 32 0.3988765719254417 1105 33 0.3875771508332164 1105 34 0.3770185134159121 1105 35 0.37026212399779723 1105 36 0.3803720751836508 1105 37 0.5351512205150399 1105 38 0.49543079504838516 1105 39 0.5761925932771135 1105 40 0.5219048405204205 1105 41 0.496846634196082 1105 42 0.4741753287412038 1105 43 0.5617169469177341 1105 44 0.5239411975347608 1105 45 0.6687553212482342 1105 46 0.6519772190045359 1105 47 0.6585332902182017 1105 48 0.6709666226412239 1105 49 0.6805440589915861 1105 50 0.6735810274667813 1106 1 0.3499755065168344 1106 2 0.37583398037562077 1106 3 0.3068209740739256 1106 4 0.3680252301882163 1106 5 0.34234001474005993 1106 6 0.37120951212839515 1106 7 0.32360399322840294 1106 8 0.42113682283727627 1106 9 0.41755757258532356 1106 10 0.5130268369537632 1106 11 0.3514663808780014 1106 12 0.5137824953557768 1106 13 0.528237798962877 1106 14 0.5849518744451814 1106 15 0.5484012660687938 1106 16 0.4203428605185451 1106 17 0.6983057690924355 1106 18 0.4625405720787441 1106 19 0.5732936477436184 1106 20 0.42176794431453857 1106 21 0.5609807878263453 1106 22 0.48273452636607317 1106 23 0.37945425793307663 1106 24 0.3554872417880759 1106 25 0.4380830360374972 1106 26 0.5455614974779266 1106 27 0.6577826183790094 1106 28 0.5831760755377218 1106 29 0.5188631612005707 1106 30 0.5647607969584882 1106 31 0.43017739126221954 1106 32 0.504473743685999 1106 33 0.48954637936707 1106 34 0.6295299737432813 1106 35 0.46375445531842985 1106 36 0.5035304130112479 1106 37 0.5232184267763031 1106 38 0.5639283775892068 1106 39 0.5342506706468413 1106 40 0.48372914288053437 1106 41 0.518068625683874 1106 42 0.46294444846810023 1106 43 0.4897397220522137 1106 44 0.4930162664362001 1106 45 0.5607015185637323 1106 46 0.6656613757764234 1106 47 0.5314946769446252 1106 48 0.49117261171135596 1106 49 0.4424653218938204 1106 50 0.5078433820905133 1107 1 0.16508712275371096 1107 2 0.0246111908305906 1107 3 0.1001875180537084 1107 4 0.06564350386772588 1107 5 0.03594259005789269 1107 6 0.062099567233261155 1107 7 0.12353069508744596 1107 8 0.26006454412727464 1107 9 0.30225725481420085 1107 10 0.2623408893758352 1107 11 0.2222232021991104 1107 12 0.07735460613376688 1107 13 0.23075389662956525 1107 14 0.007494681666180725 1107 15 0.17329790102012055 1107 16 -0.0011012466892523776 1107 17 0.19387172056175928 1107 18 0.140865194823661 1107 19 -0.01328437655749326 1107 20 0.10117929950400395 1107 21 0.10013429724507716 1107 22 -0.1347853249992994 1107 23 -0.16620407544530025 1107 24 -0.08803947458646633 1107 25 0.011713180515648958 1107 26 0.23945386811429614 1107 27 0.09194078742850564 1107 28 0.05443640979062536 1107 29 0.21681318103070168 1107 30 0.1901315020490273 1107 31 0.18263209401483493 1107 32 0.09215149746859197 1107 33 0.11106082499454573 1107 34 0.17415330545777863 1107 35 0.15000199456755325 1107 36 0.0023079194383512913 1107 37 0.0559098351686842 1107 38 0.04089621262514953 1107 39 0.1237898317485886 1107 40 0.1953457400709624 1107 41 0.3572884585960239 1107 42 0.16405249090586693 1107 43 0.16439956643264253 1107 44 0.38264774510523125 1107 45 0.3619956116549474 1107 46 0.13153527466359094 1107 47 0.15996536836897235 1107 48 0.14257059101955605 1107 49 0.2679211640104455 1107 50 0.4021030927430189 1108 1 -0.1500223091056725 1108 2 -0.1360714919820154 1108 3 -0.1091573273804336 1108 4 -0.24288212586628788 1108 5 -0.1952089465969209 1108 6 -0.31707002168388243 1108 7 -0.3188463676413633 1108 8 -0.16911204816827308 1108 9 -0.3341911912584905 1108 10 -0.292334991181022 1108 11 -0.33781533684520326 1108 12 -0.4360124085636343 1108 13 -0.3971496343176426 1108 14 -0.4346860609558618 1108 15 -0.4129829692830782 1108 16 -0.3786874540178218 1108 17 -0.39991245327303204 1108 18 -0.38711169411111257 1108 19 -0.36973290949379134 1108 20 -0.5545186232780921 1108 21 -0.4432521540198806 1108 22 -0.4553152130546607 1108 23 -0.6274347172846788 1108 24 -0.4101054703820992 1108 25 -0.33871854414542923 1108 26 -0.5851505913464123 1108 27 -0.5248908908607604 1108 28 -0.6334875775363074 1108 29 -0.6533397147890128 1108 30 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-2.332431612656883 2216 49 -2.288007299755204 2216 50 -2.429683276614771 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 136974.0 3 6907.0 4 12906.0 5 10266.0 6 8606.0 7 7516.0 8 6751.0 9 6422.0 10 6286.0 11 6426.0 12 6542.0 13 6703.0 14 7184.0 15 7529.0 16 8055.0 17 8751.0 18 9168.0 19 10374.0 20 12858.0 21 16569.0 22 19961.0 23 23052.0 24 24807.0 25 27212.0 26 30465.0 27 34400.0 28 39825.0 29 45922.0 30 53154.0 31 64498.0 32 79015.0 33 98028.0 34 125961.0 35 164442.0 36 217932.0 37 300695.0 38 475489.0 39 880427.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.49736131208719 15.790337022050787 12.517971392836596 26.19433027302542 2 16.201353595673602 19.129230485618002 37.42866070296598 27.240755215742414 3 19.968904454410282 22.857888298304253 27.152428074911512 30.020779172373953 4 13.416002641016153 15.339658105550127 34.56170313594045 36.682636117493274 5 15.202882799901769 36.5420643472138 32.86036496209487 15.394687890789562 6 34.61357561307267 33.76722863609209 16.90242461577763 14.716771135057606 7 30.792969403540845 30.455629030696908 20.09437015439093 18.657031411371317 8 28.304957048888596 33.08438043125743 18.91458840943677 19.696074110417204 9 27.086550212588456 16.043759093470396 17.288554152343863 39.58113654159728 10 15.629231140194063 28.753344585886637 31.80550266485961 23.81192160905969 11 35.81080586677558 22.04959978394386 22.206752685568475 19.932841663712082 12 24.599353830300124 23.813545481186203 28.47870044915214 23.108400239361533 13 28.838524895784584 20.474583013610783 24.6283984137105 26.05849367689413 14 22.784550350924285 19.92955903048373 25.49897904166621 31.786911576925775 15 24.34336590601652 27.460637876445986 22.29733707588241 25.898659141655084 16 24.40310352902357 26.5124149143955 23.702960100905273 25.381521455675653 17 24.14705862786969 26.862680530331755 25.03961522342188 23.950645618376672 18 24.119426795024204 26.289767741686553 25.9105426634034 23.680262799885842 19 24.744833211417447 25.748235878797317 26.289256333056237 23.217674576729003 20 25.378366627156968 25.25634453344014 24.814297210291507 24.550991629111387 21 25.461661716013502 25.360885315191585 24.65967586529965 24.517777103495266 22 24.95703825649683 24.977733090540564 25.17929145812407 24.885937194838537 23 24.95047877997011 25.459378961489705 24.99032860136108 24.599813657179105 24 24.439382289993137 25.476661874735303 25.58428903031426 24.499666804957304 25 24.86296189949863 25.04026715900013 25.03660726435852 25.06016367714273 26 24.64366610682431 25.26104535255429 25.413626793647076 24.681661746974324 27 24.54602194052623 25.251167590780643 25.425765579576826 24.777044889116304 28 23.41025497681303 25.281185277471636 26.004679987860786 25.303879757854553 29 24.06139171455408 25.933234780253585 25.07623556515134 24.929137940041 30 24.635103887678206 25.439755127842563 25.493487265649694 24.431653718829537 31 24.597175220546607 25.29651802125736 25.906684220364994 24.199622537831036 32 24.914294001095627 25.389930281034516 24.789597468683674 24.90617824918618 33 24.107309168125767 25.922992692960655 24.859799841405845 25.109898297507733 34 24.402600568820382 25.710767707951877 25.02922762893168 24.857404094296065 35 24.644952700275415 25.467641932436567 25.448881704120595 24.43852366316742 36 24.72683184816341 25.832210724490167 25.055465192473363 24.385492234873055 37 24.81168743690344 25.025837365137605 25.523125564438736 24.63934963352022 38 25.052867201713568 25.24990105069198 24.908251540782082 24.78898020681237 39 24.870602815754577 25.376450094979415 24.87642365175302 24.876523437512994 40 24.488831988036186 25.093109730278652 25.7020109439898 24.71604733769536 41 24.356058741597366 25.5635899475721 25.47658703816288 24.60376427266765 42 24.347318269767353 25.630831999180554 25.445026143186578 24.57682358786552 43 24.472887884976668 24.628696037085334 26.378436550829875 24.519979527108124 44 24.963426261723303 24.993556570319516 25.24996350108865 24.793053666868534 45 24.222719101467337 25.377641897118064 25.652281480466637 24.74735752094796 46 24.061579735628914 25.75004455841941 25.24530407463469 24.943071631316986 47 24.778677779721292 25.327070046961346 25.091797759609253 24.802454413708112 48 24.569024138904528 25.541523418159063 25.691558183528564 24.19789425940785 49 25.23230886330014 24.842525432188424 25.147315990930103 24.777849713581336 50 24.745674563865087 25.26049382346574 24.900964961897323 25.092866650771846 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1838.0 1 1337.0 2 836.0 3 850.0 4 864.0 5 901.0 6 938.0 7 960.0 8 982.0 9 1101.0 10 1220.0 11 1403.0 12 1586.0 13 2122.5 14 2659.0 15 3290.0 16 3921.0 17 4148.5 18 4376.0 19 4287.5 20 4199.0 21 4233.5 22 4268.0 23 4820.0 24 5372.0 25 6691.5 26 8011.0 27 10072.0 28 12133.0 29 16114.5 30 20096.0 31 25351.5 32 30607.0 33 39403.0 34 48199.0 35 65158.5 36 82118.0 37 92734.0 38 103350.0 39 122897.5 40 142445.0 41 165044.0 42 187643.0 43 195352.5 44 203062.0 45 214634.0 46 226206.0 47 247612.5 48 269019.0 49 274739.0 50 280459.0 51 270100.5 52 259742.0 53 248376.5 54 237011.0 55 236495.0 56 235979.0 57 212472.5 58 188966.0 59 167323.0 60 145680.0 61 127233.5 62 108787.0 63 90499.5 64 72212.0 65 58715.0 66 45218.0 67 36355.0 68 27492.0 69 22012.5 70 16533.0 71 13197.5 72 9862.0 73 8063.0 74 6264.0 75 4800.5 76 3337.0 77 2663.0 78 1989.0 79 1573.5 80 1158.0 81 885.0 82 612.0 83 496.0 84 380.0 85 287.5 86 195.0 87 152.0 88 109.0 89 81.5 90 54.0 91 40.0 92 26.0 93 22.0 94 18.0 95 14.0 96 10.0 97 10.0 98 10.0 99 18.5 100 27.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08666663563910244 2 0.09630734309416178 3 0.1045850539779886 4 0.10551588090468397 5 0.0978698025782576 6 0.05804370764321935 7 0.05382174265427958 8 0.058276414374893205 9 0.059838873858989026 10 0.04497888685067342 11 0.04827002491291781 12 0.0580769514620299 13 0.05202657643850991 14 0.05282442808996309 15 0.052226039351373196 16 0.056315029065070785 17 0.055118251587891 18 0.0559825908769653 19 0.06339596247171782 20 0.06864848584378463 21 0.0802505786086664 22 0.08300981556994201 23 0.05953967948969408 24 0.07749134164739079 25 0.08400713013425848 26 0.08211223246205718 27 0.07699268436523254 28 0.09780331494063652 29 0.08613473453813365 30 0.08071599207201409 31 0.0559825908769653 32 0.05249198990185759 33 0.055251226863133204 34 0.050031947309876944 35 0.05711288071652398 36 0.05611556615220749 37 0.058941290751104186 38 0.0494668023900976 39 0.05442013139286947 40 0.055816371782912544 41 0.04261857571512441 42 0.03909473092120617 43 0.03906148710239561 44 0.037864709625215834 45 0.028722659452314735 46 0.026262616860334074 47 0.030717288580947702 48 0.025763959578175833 49 0.025498009027691435 50 0.026894249417734512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3008078.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.30668199356716 #Duplication Level Percentage of deduplicated Percentage of total 1 74.2153517424278 32.14020636950051 2 12.235244735900016 10.597357057821773 3 5.215046516923814 6.775390832702391 4 2.601279907294535 4.506112068858411 5 1.5391047824324249 3.3326760683789693 6 0.9630491875398458 2.5023878945370797 7 0.6345146394404341 1.923510659735686 8 0.43184810522726885 1.4961526850081488 9 0.3205146342467764 1.2492382805648672 >10 1.5811349218276871 12.296526022951804 >50 0.12804833086668177 3.846715197646644 >100 0.11303670671118447 10.11739475494409 >500 0.015511900154157562 4.649586177981754 >1k 0.006313889007440011 4.566745929367865 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG 4402 0.14633929040403873 No Hit CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC 4392 0.14600685221593324 No Hit CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC 3936 0.13084767083832266 No Hit CTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG 3158 0.10498397980371518 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3297527524219784E-4 2 0.0 0.0 0.0 0.0 1.3297527524219784E-4 3 0.0 0.0 0.0 0.0 1.3297527524219784E-4 4 0.0 0.0 0.0 0.0 1.3297527524219784E-4 5 0.0 0.0 0.0 0.0 1.3297527524219784E-4 6 0.0 0.0 0.0 0.0 1.662190940527473E-4 7 0.0 0.0 0.0 0.0 1.662190940527473E-4 8 0.0 0.0 0.0 0.0 1.662190940527473E-4 9 0.0 0.0 0.0 3.324381881054946E-5 1.662190940527473E-4 10 0.0 0.0 0.0 3.324381881054946E-5 1.662190940527473E-4 11 0.0 0.0 0.0 3.324381881054946E-5 1.662190940527473E-4 12 0.0 0.0 0.0 3.324381881054946E-5 4.986572821582419E-4 13 0.0 0.0 0.0 3.324381881054946E-5 5.319011009687914E-4 14 0.0 0.0 0.0 3.324381881054946E-5 5.319011009687914E-4 15 0.0 0.0 0.0 3.324381881054946E-5 7.978516514531871E-4 16 0.0 0.0 0.0 3.324381881054946E-5 8.64339289074286E-4 17 0.0 0.0 0.0 3.324381881054946E-5 8.64339289074286E-4 18 0.0 0.0 0.0 3.324381881054946E-5 8.975831078848355E-4 19 0.0 0.0 0.0 3.324381881054946E-5 8.975831078848355E-4 20 0.0 0.0 0.0 3.324381881054946E-5 0.0011635336583692311 21 0.0 0.0 0.0 6.648763762109892E-5 0.00123002129599033 22 0.0 0.0 0.0 6.648763762109892E-5 0.0012632651148008795 23 0.0 0.0 0.0 1.3297527524219784E-4 0.0013297527524219785 24 0.0 0.0 0.0 4.32169644537143E-4 0.0013297527524219785 25 0.0 0.0 0.0 5.319011009687914E-4 0.0013297527524219785 26 0.0 0.0 0.0 7.978516514531871E-4 0.0014294842088536268 27 3.324381881054946E-5 0.0 0.0 0.0011302898395586817 0.0014959718464747256 28 3.324381881054946E-5 3.324381881054946E-5 0.0 0.002094360585064616 0.0014959718464747256 29 3.324381881054946E-5 3.324381881054946E-5 0.0 0.00369006388797099 0.0014959718464747256 30 3.324381881054946E-5 3.324381881054946E-5 0.0 0.006515788486867694 0.0015292156652852752 31 3.324381881054946E-5 3.324381881054946E-5 0.0 0.013962403900430773 0.0015624594840958246 32 3.324381881054946E-5 3.324381881054946E-5 0.0 0.023104454073331875 0.0016289471217169236 33 3.324381881054946E-5 3.324381881054946E-5 0.0 0.034706546838213635 0.0016289471217169236 34 3.324381881054946E-5 3.324381881054946E-5 0.0 0.04936707093366595 0.001662190940527473 35 3.324381881054946E-5 3.324381881054946E-5 0.0 0.06828280383686859 0.001662190940527473 36 3.324381881054946E-5 3.324381881054946E-5 0.0 0.09251754774975915 0.001662190940527473 37 3.324381881054946E-5 3.324381881054946E-5 0.0 0.12120696338326334 0.001662190940527473 38 3.324381881054946E-5 3.324381881054946E-5 0.0 0.1551821462076449 0.001728678578148572 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5450 0.0 17.484991 1 TATAGGC 1260 0.0 17.289724 5 TTATAGG 1260 0.0 16.941565 4 ATAGGCC 1305 0.0 16.51858 6 CTTATAG 1310 0.0 16.295753 3 TAACGGG 85 0.0029803491 15.53017 30 CTAGTAC 185 7.545168E-9 15.464847 3 TATCATA 805 0.0 15.036043 2 TCATACT 1000 0.0 14.964465 4 GTACATG 10180 0.0 14.851932 1 TAGGCCT 1455 0.0 14.815634 7 TACATGG 10300 0.0 14.529091 2 TATACAG 445 0.0 14.340402 5 GTATTAA 340 0.0 14.240255 1 TCGCACG 155 4.5584547E-6 14.197549 22 TACTAGT 140 2.7507125E-5 14.14755 2 AGAATAT 1760 0.0 14.128276 25 ACATGGG 10470 0.0 14.104244 3 AATATCG 1670 0.0 14.093448 33 CGGTAAG 125 1.6709241E-4 14.08139 16 >>END_MODULE