FastQCFastQC Report
Mon 6 Feb 2017
SRR2077121_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077121_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3008078
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC50050.16638531314680005No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC41330.13739670314400093No Hit
CTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG35800.11901287134176708No Hit
GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG34780.11562200182309101No Hit
ACTCAGTAGTGACCATTTTCTTGAAGTCATTCTTGTAGAGGGCATGGTGA31820.10578183145516838No Hit
TTCTTGTAGAGGGCATGGTGATTTCCTTGTATATTGGAATAATTGTGGTA30160.10026335753261717No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATCG508.337011E-526.403945
TATACCG550.004481263520.0029855
GTATCAA56600.019.1650871
TACACCG1557.221388E-1018.4543675
TATAGGC11350.017.4475385
ACTTTAT12900.017.227091
CTTATAG12600.017.1133823
AATATCG14150.016.94445433
ATAGGCC11450.016.717826
GGTATCA33900.016.680681
GACCGTT1602.12749E-816.50328811
CGTACAC1602.129309E-816.502193
TTATAGG11950.016.3871124
ATATCGA14700.016.31047834
TTTACGC1351.0914919E-616.28951841
CGAAAAC15450.015.94423738
TATCGAA15250.015.72170835
CCGCGCT1158.212387E-515.3068879
TTACGCA1452.2981258E-615.16610242
TTTATTC15700.015.1357663
Produced by FastQC (version 0.11.5)