##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077121_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3008078 Sequences flagged as poor quality 0 Sequence length 50 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.42774622200621 31.0 31.0 34.0 27.0 34.0 2 30.540646552383283 31.0 31.0 34.0 27.0 34.0 3 30.595265149374452 31.0 31.0 34.0 27.0 34.0 4 34.135108198657086 37.0 35.0 37.0 30.0 37.0 5 33.641143613962136 37.0 35.0 37.0 28.0 37.0 6 33.55910883959791 37.0 35.0 37.0 28.0 37.0 7 33.38652654618664 37.0 35.0 37.0 27.0 37.0 8 33.31106540455401 37.0 35.0 37.0 27.0 37.0 9 34.73643934764989 39.0 35.0 39.0 27.0 39.0 10 34.592991604606 39.0 35.0 39.0 27.0 39.0 11 34.598178970093194 39.0 35.0 39.0 27.0 39.0 12 34.523135370824825 39.0 35.0 39.0 27.0 39.0 13 34.47996029358281 39.0 35.0 39.0 27.0 39.0 14 35.53295227051958 40.0 36.0 41.0 26.0 41.0 15 35.53278771361647 40.0 36.0 41.0 26.0 41.0 16 35.508718856359444 40.0 36.0 41.0 26.0 41.0 17 35.48605189094166 40.0 36.0 41.0 26.0 41.0 18 35.39566095028121 40.0 36.0 41.0 25.0 41.0 19 35.43572540339712 40.0 35.0 41.0 25.0 41.0 20 35.40742061874725 40.0 35.0 41.0 25.0 41.0 21 35.37154388948691 40.0 35.0 41.0 25.0 41.0 22 35.293626694520555 39.0 35.0 41.0 25.0 41.0 23 35.24744936800176 39.0 35.0 41.0 25.0 41.0 24 35.21972136360826 39.0 35.0 41.0 25.0 41.0 25 35.15161043031464 39.0 35.0 41.0 24.0 41.0 26 35.097013774243884 39.0 35.0 41.0 24.0 41.0 27 34.98608413744591 39.0 35.0 41.0 24.0 41.0 28 34.96135472550911 39.0 35.0 41.0 24.0 41.0 29 34.930000152921565 39.0 35.0 41.0 24.0 41.0 30 34.85021133095618 39.0 35.0 41.0 24.0 41.0 31 34.765264065625956 39.0 34.0 41.0 23.0 41.0 32 34.70311740586514 39.0 34.0 41.0 23.0 41.0 33 34.5884022954192 39.0 34.0 41.0 22.0 41.0 34 34.54564808492333 39.0 34.0 41.0 22.0 41.0 35 34.472888003569054 39.0 34.0 41.0 21.0 41.0 36 34.402864220941076 39.0 34.0 41.0 19.0 41.0 37 34.31955853538372 39.0 34.0 41.0 19.0 41.0 38 34.16156928111572 39.0 34.0 41.0 18.0 41.0 39 34.01664152325837 39.0 33.0 41.0 16.0 41.0 40 34.00559992127864 39.0 33.0 41.0 16.0 41.0 41 33.88954176055275 38.0 33.0 41.0 15.0 41.0 42 34.02563297893206 39.0 33.0 41.0 15.0 41.0 43 34.01602119359937 39.0 34.0 41.0 15.0 41.0 44 33.94801664052594 39.0 33.0 41.0 14.0 41.0 45 33.80373580738265 39.0 33.0 41.0 9.0 41.0 46 33.70041435095766 39.0 33.0 41.0 8.0 41.0 47 33.57175512071163 39.0 33.0 41.0 2.0 41.0 48 33.41283636926968 38.0 33.0 41.0 2.0 41.0 49 33.163594162119466 38.0 33.0 41.0 2.0 41.0 50 33.046961215766345 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2940051695994974 1101 2 0.5741183392751807 1101 3 0.45082566319632633 1101 4 0.4987505182866201 1101 5 0.6589544398319447 1101 6 0.7146477324341092 1101 7 0.7473444640933735 1101 8 0.6557661494289704 1101 9 0.8606544791309787 1101 10 0.8020751476565309 1101 11 0.9094602027609966 1101 12 0.7934197466690378 1101 13 0.8733524736301987 1101 14 1.0830515950944033 1101 15 1.0567528742771302 1101 16 1.0393838876037051 1101 17 1.1240581919708248 1101 18 1.1689704519834834 1101 19 1.0641490336168928 1101 20 0.9572235171989121 1101 21 1.1585416968725326 1101 22 1.224795532632939 1101 23 1.2694391538719074 1101 24 1.2177103813986179 1101 25 1.2896014044080601 1101 26 1.1714302657297964 1101 27 1.2384085592566336 1101 28 1.2040287214459582 1101 29 1.2030937825256487 1101 30 1.2262843599636426 1101 31 1.1581767848199291 1101 32 1.254682841539875 1101 33 1.1659829651588183 1101 34 1.2382623136190034 1101 35 1.186911593124087 1101 36 1.20601413120621 1101 37 1.1334121771024002 1101 38 1.2216964372066528 1101 39 1.4515158532705286 1101 40 1.4982632275523144 1101 41 1.4661807795483597 1101 42 1.4975215855415627 1101 43 1.456040223619354 1101 44 1.3607243807039566 1101 45 1.322859330754909 1101 46 1.3552856442716674 1101 47 1.1909016387798914 1101 48 1.4094363771772578 1101 49 1.7564829248766856 1101 50 1.5740836716220983 1102 1 0.3044309596195056 1102 2 0.35771351140967766 1102 3 0.35005201051164647 1102 4 0.35830412916494225 1102 5 0.4427470334620267 1102 6 0.4841918845882631 1102 7 0.5473136414608035 1102 8 0.5199680344471886 1102 9 0.5915996189915518 1102 10 0.6363408387492555 1102 11 0.626007164888783 1102 12 0.6404560975642042 1102 13 0.6167100580941138 1102 14 0.6197054182296284 1102 15 0.6690050780492882 1102 16 0.7493461402451658 1102 17 0.7277321207691614 1102 18 0.762925345894196 1102 19 0.5957095203841192 1102 20 0.6448789837471409 1102 21 0.6959389029321983 1102 22 0.726145462579403 1102 23 0.7270462855564261 1102 24 0.6804375716138864 1102 25 0.6932339138541153 1102 26 0.6755680544155425 1102 27 0.6600096498894601 1102 28 0.7019121418643621 1102 29 0.735953980002428 1102 30 0.7659706878455026 1102 31 0.7667000950693108 1102 32 0.7129209387744524 1102 33 0.7659604816839405 1102 34 0.6883614390210511 1102 35 0.8133159464408379 1102 36 0.7306821428884049 1102 37 0.6843711111276818 1102 38 0.9022198506588666 1102 39 0.8701072451404528 1102 40 0.7882028400717402 1102 41 0.9155526474689992 1102 42 0.9016178166569375 1102 43 0.7944116828752001 1102 44 0.7828179884401081 1102 45 0.8538031345073449 1102 46 0.8783680641754401 1102 47 0.9814309817878737 1102 48 0.9191971078533356 1102 49 1.0310517650841433 1102 50 0.9599222153932345 1103 1 0.3391990287768252 1103 2 0.4010847003817446 1103 3 0.3734383774586547 1103 4 0.2262310505224363 1103 5 0.36407627412795307 1103 6 0.44452665814091574 1103 7 0.4266101259973567 1103 8 0.48918376512477835 1103 9 0.5605381402398777 1103 10 0.6661910305459458 1103 11 0.6432852968844216 1103 12 0.5294079257023157 1103 13 0.5909310462280075 1103 14 0.7715892912335889 1103 15 0.6558090092536304 1103 16 0.6240438155788226 1103 17 0.5827651933505251 1103 18 0.685370435614189 1103 19 0.6379949985866062 1103 20 0.5680845419165408 1103 21 0.5010544999967266 1103 22 0.7017177551149913 1103 23 0.6846053021776868 1103 24 0.672944754571688 1103 25 0.7073764085003873 1103 26 0.7458418481725673 1103 27 0.8391147772488239 1103 28 0.6940083767554555 1103 29 0.7041621710858479 1103 30 0.7105333018058886 1103 31 0.5858099022345158 1103 32 0.7048456575256523 1103 33 0.7804276954162859 1103 34 0.7338791435888368 1103 35 0.680381415166508 1103 36 0.6985953345829614 1103 37 0.7157672504134425 1103 38 0.7307960092666121 1103 39 0.7994144455114878 1103 40 0.8302309917409829 1103 41 0.8296389677016691 1103 42 0.7972520768541429 1103 43 0.7313702402224962 1103 44 0.8178996841204551 1103 45 0.8753199859942526 1103 46 0.8607518183006206 1103 47 0.8658382077764557 1103 48 0.9004610363452272 1103 49 0.9795379023104474 1103 50 0.8959214753309155 1104 1 0.2648815667538713 1104 2 0.33524172249003925 1104 3 0.30345553044642415 1104 4 0.30082800007909327 1104 5 0.3172061507696995 1104 6 0.3764592920518979 1104 7 0.29704718821882636 1104 8 0.352936150681991 1104 9 0.45462899351468167 1104 10 0.4402707682753544 1104 11 0.3165352700229249 1104 12 0.40885190540013383 1104 13 0.4306570972802106 1104 14 0.6249194139778638 1104 15 0.5450005164141345 1104 16 0.5875604594771673 1104 17 0.6096449228935725 1104 18 0.6306033504197401 1104 19 0.6289471492975451 1104 20 0.5820793845081411 1104 21 0.5976004945630251 1104 22 0.7151887984157312 1104 23 0.7026523942282168 1104 24 0.7197454857161958 1104 25 0.7935554621568741 1104 26 0.6046843348825135 1104 27 0.614982403766497 1104 28 0.6606361582000417 1104 29 0.6184563915138952 1104 30 0.6857174310071557 1104 31 0.705144683530726 1104 32 0.7623136027998214 1104 33 0.7637735641172583 1104 34 0.7860707223800176 1104 35 0.7792867450001708 1104 36 0.7661648035438517 1104 37 0.6985643770001886 1104 38 0.7693915108603946 1104 39 0.9071370634079017 1104 40 0.7846371095120404 1104 41 0.8259458309433683 1104 42 0.8965174787277945 1104 43 0.8259857242155988 1104 44 0.8141880001345214 1104 45 0.8337901850763529 1104 46 0.8974280016137399 1104 47 0.8848609479566534 1104 48 0.8379789440132939 1104 49 0.9567235331975965 1104 50 0.9233240448254563 1105 1 0.22618066333503606 1105 2 0.24345709423301187 1105 3 0.2853976225495529 1105 4 0.20377471873318598 1105 5 0.269408246103076 1105 6 0.3077241116912859 1105 7 0.2576057048373812 1105 8 0.220088570377861 1105 9 0.40559261912802214 1105 10 0.39317072548185905 1105 11 0.4052409132422383 1105 12 0.42432547978148705 1105 13 0.4060903365583357 1105 14 0.42328972108189333 1105 15 0.48052374846744783 1105 16 0.5148410077130521 1105 17 0.5153581147053785 1105 18 0.4148061565679342 1105 19 0.4034894125480548 1105 20 0.4721255506527626 1105 21 0.388707173875666 1105 22 0.5749868310148187 1105 23 0.5849940002766729 1105 24 0.5498544904809677 1105 25 0.5856553924993335 1105 26 0.6390901850339361 1105 27 0.624621654158517 1105 28 0.5590662928090424 1105 29 0.5189612590028716 1105 30 0.4186253489492131 1105 31 0.5337651203526903 1105 32 0.4738806189874083 1105 33 0.5188000925831417 1105 34 0.49053349405298263 1105 35 0.5207692880510635 1105 36 0.4996444252629715 1105 37 0.5710156141679619 1105 38 0.5142209696452653 1105 39 0.6478830188410001 1105 40 0.43438359783445435 1105 41 0.4641265850644771 1105 42 0.49228533594273927 1105 43 0.38322945016809484 1105 44 0.4044809331119623 1105 45 0.4513013280434208 1105 46 0.45371130248265956 1105 47 0.5043294045068336 1105 48 0.4108392590101886 1105 49 0.4662773695903297 1105 50 0.46346767076084916 1106 1 0.1832614112782771 1106 2 0.18737229718963988 1106 3 0.2016280925676348 1106 4 0.12951533718943864 1106 5 0.16518246045146867 1106 6 0.1183281911401437 1106 7 0.19733418795440372 1106 8 0.1253694904649052 1106 9 0.1135477575755175 1106 10 0.18511947833968634 1106 11 0.042602041695580795 1106 12 0.08661290025899149 1106 13 0.12119653567286548 1106 14 0.10608565564217542 1106 15 0.09465971982665877 1106 16 0.12936234073160335 1106 17 0.09195921604261059 1106 18 0.06003857608082086 1106 19 0.17718205954658117 1106 20 0.25737692884703023 1106 21 0.19954359140432132 1106 22 0.2584462355628645 1106 23 0.21512510666010343 1106 24 0.22554005884792616 1106 25 0.1277447112195489 1106 26 0.24773805200131704 1106 27 0.3656551763857081 1106 28 0.22748723556228612 1106 29 0.20430430569218316 1106 30 0.20150845884031554 1106 31 0.14527307614437035 1106 32 0.1508270981187536 1106 33 0.2630842734804091 1106 34 0.3076533678347957 1106 35 0.24594405911927453 1106 36 0.28349992121716383 1106 37 0.2655060900059709 1106 38 0.11742804727050782 1106 39 0.3802406734781201 1106 40 0.33094857377815146 1106 41 0.29851521614077825 1106 42 0.2735551880156777 1106 43 0.16942059705444734 1106 44 0.2560807666088962 1106 45 0.28451992679498517 1106 46 0.2343979259487412 1106 47 0.2890992020041594 1106 48 0.30949157014953954 1106 49 0.40376611185258326 1106 50 0.3184720692508449 1107 1 0.13535034395388124 1107 2 0.13888948892373065 1107 3 0.13305829938008884 1107 4 0.0578397669090549 1107 5 0.07649505566119785 1107 6 0.03538599140836851 1107 7 0.16203063250556937 1107 8 0.08739536563518158 1107 9 0.18703326176147073 1107 10 0.18751736731204716 1107 11 0.1869713263772681 1107 12 0.1946628016745393 1107 13 0.12520489907310406 1107 14 0.10528897588324782 1107 15 0.11247814272759626 1107 16 0.19682635631157552 1107 17 0.20431551192311304 1107 18 0.33004841522382833 1107 19 0.3328256946916994 1107 20 0.1863376986422267 1107 21 0.2074702397310162 1107 22 0.24515773042543287 1107 23 0.3056238608748103 1107 24 0.23455845827698596 1107 25 0.2428804427506961 1107 26 0.2350203870206613 1107 27 0.19573706286572445 1107 28 0.2897169197137046 1107 29 0.3158287912089861 1107 30 0.22206938521762964 1107 31 0.25098878693687965 1107 32 0.20659918516750508 1107 33 0.2889616313809711 1107 34 0.21917237143304646 1107 35 0.32370005506419375 1107 36 0.16475281498813388 1107 37 0.16910449340362277 1107 38 0.23072064454431995 1107 39 0.2681394283619838 1107 40 0.23447993305460102 1107 41 0.22688185405954187 1107 42 0.23346756482371234 1107 43 0.18868310657381215 1107 44 0.26896533413313506 1107 45 0.2435138892209352 1107 46 0.17912641784721473 1107 47 0.20879951864286284 1107 48 0.27739429363359136 1107 49 0.34813261696994147 1107 50 0.28512244458210745 1108 1 -0.054231193119381516 1108 2 -0.06398380889408273 1108 3 -0.06643633817023087 1108 4 -0.04829549325956606 1108 5 -0.13103613609999343 1108 6 -0.15020144838116067 1108 7 -0.1555678593417369 1108 8 -0.19337784242078015 1108 9 -0.15964750019607976 1108 10 -0.20150422522696942 1108 11 -0.25180933240468306 1108 12 -0.2226461754371556 1108 13 -0.2505419713521988 1108 14 -0.14796559053245772 1108 15 -0.18850338263578692 1108 16 -0.18844193488831706 1108 17 -0.17936952297225162 1108 18 -0.18287187831169405 1108 19 -0.3004253353728785 1108 20 -0.3618684717977061 1108 21 -0.3090407364463914 1108 22 -0.2693761381547688 1108 23 -0.22060556612689197 1108 24 -0.28255488361408254 1108 25 -0.3881868445564507 1108 26 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23 -0.8227458402122991 2215 24 -0.8119879950008908 2215 25 -0.7522291936402397 2215 26 -0.7782461437061059 2215 27 -0.8368047115884991 2215 28 -0.8137418523306792 2215 29 -0.9790720931503145 2215 30 -0.93043543536632 2215 31 -0.9228703197889843 2215 32 -0.88559196608756 2215 33 -0.8892052277503737 2215 34 -1.0309151384490107 2215 35 -0.8637147277555925 2215 36 -0.8580753217988999 2215 37 -0.9357913645380407 2215 38 -0.9251589516401637 2215 39 -0.9061659094718095 2215 40 -0.844337735479975 2215 41 -0.9683406688564702 2215 42 -0.9036725258213636 2215 43 -0.9363189925221036 2215 44 -0.880363044844735 2215 45 -0.8583317989425936 2215 46 -0.8987813435578857 2215 47 -0.9025001714409058 2215 48 -1.0136699325059695 2215 49 -0.8803139069516774 2215 50 -0.9104109075656481 2216 1 -0.6685036244030123 2216 2 -0.6580279645651892 2216 3 -0.6704504263342983 2216 4 -0.6601985659159979 2216 5 -0.8782017471673456 2216 6 -0.8962512371805929 2216 7 -0.920574896577719 2216 8 -0.8811389210372838 2216 9 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-1.7791235247808004 2216 46 -1.98990839888965 2216 47 -2.092694405120401 2216 48 -1.9996107901888571 2216 49 -1.9316813522110081 2216 50 -1.9338163560302597 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 82204.0 3 13197.0 4 21342.0 5 13081.0 6 9871.0 7 7990.0 8 6799.0 9 6061.0 10 5704.0 11 5759.0 12 5732.0 13 5941.0 14 6336.0 15 6758.0 16 7268.0 17 8088.0 18 8783.0 19 9585.0 20 10977.0 21 13534.0 22 16381.0 23 19659.0 24 23023.0 25 26451.0 26 29792.0 27 34175.0 28 39381.0 29 45995.0 30 54138.0 31 64617.0 32 78702.0 33 97529.0 34 123746.0 35 158877.0 36 208030.0 37 281862.0 38 439659.0 39 1011051.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87102833254378 16.503619484545506 13.803741657732232 27.82161052517848 2 15.376267832439156 19.06425679177842 36.8278580526373 28.731617323145127 3 18.585108416512224 22.569483806088613 27.835077250401003 31.010330526998164 4 12.861383567842086 15.457098412556988 34.29849673478574 37.38302128481518 5 14.790005344611007 36.361547136841665 33.46365049237271 15.384797026174626 6 34.770988622174045 34.03680875508993 16.644414257282193 14.54778836545384 7 30.355399459442207 30.387255658299374 20.387468476329314 18.86987640592911 8 28.26834759416974 33.01994965198682 18.912296687473187 19.799406066370253 9 26.741581328776952 15.516420324661981 17.50646643605428 40.235531910506786 10 15.24367856458719 28.72551641019092 32.25149256113913 23.779312464082754 11 36.01301422778491 21.506600067911307 22.402184602358005 20.07820110194578 12 24.475032623400256 23.58904625273192 28.74311117496119 23.192809948906635 13 28.817227047278454 20.06377455989101 25.030832907593076 26.088165485237457 14 22.594703363405813 19.515436743857315 25.922421319585915 31.967438573150957 15 24.195004323820925 27.366194372380765 22.60969201090933 25.82910929288898 16 24.240618009762425 26.369658315108474 23.919206366365366 25.47051730876373 17 23.93298483513969 26.61823481983912 25.404567020486795 24.044213324534397 18 23.928045666116084 25.96645022580939 26.52127810310798 23.584226004966542 19 24.364050003908115 25.426657996750446 27.030814234849405 23.178477764492037 20 25.177299185369982 24.82837159474643 25.52745738516837 24.466871834715217 21 25.143678734349994 24.974556890792186 25.41088126948144 24.47088310537638 22 24.593610312899106 24.583732439335886 26.006013197105148 24.816644050659857 23 24.85678670065294 24.958725567930866 25.69078964873163 24.493698082684563 24 24.183431061197897 25.082720310068545 26.276540091039735 24.457308537693823 25 24.513402568602547 24.76502810749725 25.745855545123813 24.975713778776388 26 24.3490878566352 24.90662058474421 26.095817540064765 24.64847401855582 27 24.21942710429256 24.960240519084884 26.167758070792164 24.652574305830395 28 23.0514277086456 24.913855919140133 26.747250722592614 25.287465649621648 29 23.868574566078372 25.503294548572185 25.770643016464927 24.857487868884515 30 24.329006258151924 25.03553425089409 26.18363808207497 24.451821408879006 31 24.29686375044354 24.999143682722977 26.744301665778202 23.959690901055282 32 24.59309513722668 24.943751924538795 25.477634780896867 24.985518157337655 33 23.974868406614416 25.41602795798322 25.57051510123463 25.038588534167726 34 24.09081901103666 25.313855807878618 25.796360482963536 24.798964698121186 35 24.48576179540064 24.912394218866325 26.108966662421736 24.4928773233113 36 24.567896637005244 25.365104149695195 25.729426918987148 24.337572294312416 37 24.605752285951787 24.68821280133001 26.17363258520366 24.532402327514546 38 24.78411616299313 24.893870135880807 25.485896564610876 24.836117136515192 39 24.6775773365544 25.042175111995952 25.475928766784435 24.804318784665217 40 24.227930419980318 24.759089820996355 26.356590951405696 24.65638880761763 41 24.042528787221414 25.208280346892874 26.015502793528256 24.733688072357452 42 24.11143607462983 25.256968494866282 26.011924096176053 24.61967133432784 43 24.230445593353718 24.293978776392784 26.901066765695536 24.57450886455796 44 24.717900161874322 24.577170234672888 25.77063182850165 24.934297774951137 45 23.943675082513955 25.068916773272125 26.21686476221885 24.77054338199507 46 23.911901578282116 25.285393327158594 25.85415202493123 24.94855306962806 47 24.629239929388532 24.80523668471847 25.65839209311139 24.907131292781607 48 24.364986095435345 25.22884769141009 26.16431821090723 24.241848002247337 49 25.054578202827127 24.519284630771434 25.64100092118898 24.78513624521246 50 24.535766823746478 25.019605887584046 25.290281829439582 25.154345459229894 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 633.0 1 437.5 2 242.0 3 366.5 4 491.0 5 535.0 6 579.0 7 532.5 8 486.0 9 612.0 10 738.0 11 1127.0 12 1516.0 13 2640.5 14 3765.0 15 5078.5 16 6392.0 17 7126.5 18 7861.0 19 7721.0 20 7581.0 21 7100.5 22 6620.0 23 6757.5 24 6895.0 25 7780.0 26 8665.0 27 10647.5 28 12630.0 29 16851.5 30 21073.0 31 26661.5 32 32250.0 33 41534.0 34 50818.0 35 68927.0 36 87036.0 37 96605.5 38 106175.0 39 124840.5 40 143506.0 41 165468.0 42 187430.0 43 193811.0 44 200192.0 45 211218.0 46 222244.0 47 243568.0 48 264892.0 49 271832.0 50 278772.0 51 268646.5 52 258521.0 53 246778.5 54 235036.0 55 234850.5 56 234665.0 57 211470.5 58 188276.0 59 165801.5 60 143327.0 61 125713.5 62 108100.0 63 90158.0 64 72216.0 65 58264.0 66 44312.0 67 35255.0 68 26198.0 69 20950.5 70 15703.0 71 12515.0 72 9327.0 73 7665.0 74 6003.0 75 4492.5 76 2982.0 77 2332.5 78 1683.0 79 1394.5 80 1106.0 81 835.0 82 564.0 83 422.5 84 281.0 85 226.0 86 171.0 87 121.0 88 71.0 89 48.5 90 26.0 91 20.0 92 14.0 93 11.5 94 9.0 95 6.0 96 3.0 97 2.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0017619223969591214 2 0.009540975998627695 3 9.973145643164837E-5 4 0.0052857671908773645 5 0.04358264646063034 6 0.030351606574031655 7 0.027060468511787263 8 0.03350976936103386 9 0.033642744636276055 10 0.03876229273310067 11 0.040391239854817594 12 0.03437410865010814 13 0.051527919156351666 14 0.04830326873172836 15 0.0491343642019921 16 0.05495203249383826 17 0.05498527631264881 18 0.05186035734445717 19 0.05029789786036133 20 0.04777136763075958 21 0.04554403177045276 22 0.04514510594472617 23 0.04567700704569496 24 0.04185396788248177 25 0.040856653318165286 26 0.04185396788248177 27 0.04148828587556573 28 0.039792851116227705 29 0.03793119726283693 30 0.037233077067815396 31 0.0337424760927077 32 0.028722659452314735 33 0.02353662371786902 34 0.021475506951614952 35 0.018982220540823744 36 0.01928141491011869 37 0.019214927272497587 38 0.014693767914262862 39 0.012632651148008794 40 0.010571534381754729 41 0.007745809782858024 42 0.007280396319510332 43 0.006682007580920441 44 0.006283081755193848 45 0.0033908695186760447 46 0.004122233532508133 47 0.0022605796791173634 48 0.002426798773170111 49 2.991943692949451E-4 50 0.0017619223969591214 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 3008078.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.86437750357852 #Duplication Level Percentage of deduplicated Percentage of total 1 73.03255826557016 30.574625892819224 2 12.848895117240305 10.758219913840698 3 5.3909306798585614 6.77063871231666 4 2.7446773735997585 4.596168387756427 5 1.5645627157538577 3.274972208017176 6 0.9833805269706271 2.4701168166459784 7 0.6625461120670315 1.9415956384371713 8 0.4673144312924968 1.5651062211599354 9 0.34445335350390743 1.2978292701115046 >10 1.6800132001974202 12.65886923744029 >50 0.1375249360419431 4.012188499651145 >100 0.12098618190056357 10.64244770911841 >500 0.015012242111008042 4.418235149955785 >1k 0.0070645845228123234 4.8507760087508816 >5k 8.027936957741278E-5 0.16821033397859214 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC 5005 0.16638531314680005 No Hit CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC 4133 0.13739670314400093 No Hit CTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG 3580 0.11901287134176708 No Hit GTACATGGGGATCCTTTGTCAGCTCCGTCTTCAAGACATCGTTTGAAAGG 3478 0.11562200182309101 No Hit ACTCAGTAGTGACCATTTTCTTGAAGTCATTCTTGTAGAGGGCATGGTGA 3182 0.10578183145516838 No Hit TTCTTGTAGAGGGCATGGTGATTTCCTTGTATATTGGAATAATTGTGGTA 3016 0.10026335753261717 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.662190940527473E-4 2 0.0 0.0 0.0 0.0 1.662190940527473E-4 3 0.0 0.0 0.0 0.0 1.662190940527473E-4 4 0.0 0.0 0.0 0.0 1.662190940527473E-4 5 0.0 0.0 0.0 0.0 1.662190940527473E-4 6 0.0 0.0 0.0 0.0 1.9946291286329678E-4 7 0.0 0.0 0.0 0.0 1.9946291286329678E-4 8 0.0 0.0 0.0 0.0 1.9946291286329678E-4 9 0.0 0.0 0.0 3.324381881054946E-5 1.9946291286329678E-4 10 0.0 0.0 0.0 3.324381881054946E-5 1.9946291286329678E-4 11 0.0 0.0 0.0 3.324381881054946E-5 1.9946291286329678E-4 12 0.0 0.0 0.0 3.324381881054946E-5 5.319011009687914E-4 13 0.0 0.0 0.0 3.324381881054946E-5 6.648763762109892E-4 14 0.0 0.0 0.0 6.648763762109892E-5 6.648763762109892E-4 15 0.0 0.0 0.0 6.648763762109892E-5 8.975831078848355E-4 16 0.0 0.0 0.0 6.648763762109892E-5 8.975831078848355E-4 17 0.0 0.0 0.0 6.648763762109892E-5 8.975831078848355E-4 18 0.0 0.0 0.0 6.648763762109892E-5 0.0010638022019375827 19 0.0 0.0 0.0 6.648763762109892E-5 0.0010638022019375827 20 0.0 0.0 0.0 6.648763762109892E-5 0.00123002129599033 21 0.0 0.0 0.0 9.973145643164839E-5 0.0013962403900430772 22 0.0 0.0 0.0 9.973145643164839E-5 0.0014959718464747256 23 0.0 0.0 0.0 1.662190940527473E-4 0.0015624594840958246 24 0.0 0.0 0.0 4.986572821582419E-4 0.0015624594840958246 25 0.0 0.0 0.0 5.983887385898902E-4 0.0015624594840958246 26 0.0 0.0 0.0 8.310954702637365E-4 0.001662190940527473 27 0.0 0.0 0.0 0.0011967774771797805 0.001662190940527473 28 0.0 0.0 0.0 0.002094360585064616 0.001662190940527473 29 0.0 0.0 0.0 0.0036568200691604407 0.0016954347593380226 30 0.0 0.0 0.0 0.006382813211625497 0.0016954347593380226 31 0.0 0.0 0.0 0.013796184806378026 0.001728678578148572 32 3.324381881054946E-5 0.0 0.0 0.023004722616900227 0.001728678578148572 33 3.324381881054946E-5 0.0 0.0 0.034640059200592536 0.001728678578148572 34 3.324381881054946E-5 0.0 0.0 0.04916760802080265 0.0018284100345802203 35 3.324381881054946E-5 0.0 0.0 0.0677509027358998 0.0018616538533907697 36 3.324381881054946E-5 0.0 0.0 0.09105481972209498 0.0018616538533907697 37 3.324381881054946E-5 0.0 0.0 0.11954477244273586 0.0018948976722013193 38 3.324381881054946E-5 0.0 0.0 0.15342022381068576 0.0019281414910118687 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGATCG 50 8.337011E-5 26.40394 5 TATACCG 55 0.0044812635 20.002985 5 GTATCAA 5660 0.0 19.165087 1 TACACCG 155 7.221388E-10 18.454367 5 TATAGGC 1135 0.0 17.447538 5 ACTTTAT 1290 0.0 17.22709 1 CTTATAG 1260 0.0 17.113382 3 AATATCG 1415 0.0 16.944454 33 ATAGGCC 1145 0.0 16.71782 6 GGTATCA 3390 0.0 16.68068 1 GACCGTT 160 2.12749E-8 16.503288 11 CGTACAC 160 2.129309E-8 16.50219 3 TTATAGG 1195 0.0 16.387112 4 ATATCGA 1470 0.0 16.310478 34 TTTACGC 135 1.0914919E-6 16.289518 41 CGAAAAC 1545 0.0 15.944237 38 TATCGAA 1525 0.0 15.721708 35 CCGCGCT 115 8.212387E-5 15.306887 9 TTACGCA 145 2.2981258E-6 15.166102 42 TTTATTC 1570 0.0 15.135766 3 >>END_MODULE