FastQCFastQC Report
Mon 6 Feb 2017
SRR2077120_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077120_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184005
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100920.4620868541967624No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51620.2363547702500681No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46500.21291160047710514No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41260.18891898141258834No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37670.17248129010693655No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30260.1385527963534882No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27970.1280674723730028No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT25970.11890998418043915No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC24480.11208765547697921No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC22430.10270123007960147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA1107.2759576E-1225.99541310
GTTCGCG708.1283203E-418.85640716
GCGTAAC1658.367351E-1118.66380111
TATCGCA600.007400951318.338924
TCGCGTA1355.539914E-817.9235849
AGTTCGC1053.782309E-516.75894710
ATATCGA5850.015.41608234
AATATCG6050.015.27003233
TTATCGG1603.8411417E-715.12025444
GAGTACT27100.014.69214312
TACCGCG1057.1654585E-414.66575717
GTACATG105400.014.3857861
GTTATCG1851.2711826E-714.26644543
GTACTTT28000.014.06341514
TACATGG105050.013.993472
AGTACTT28650.013.97406113
TATCGAA6300.013.9657935
TTGACGC950.006233162213.89769628
ACATGGG109800.013.2283763
GCGTGTA1000.008771026513.1982759
Produced by FastQC (version 0.11.5)