##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077120_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2184005 Sequences flagged as poor quality 0 Sequence length 50 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.29961149356343 31.0 30.0 34.0 23.0 34.0 2 29.405773338431 31.0 30.0 34.0 23.0 34.0 3 29.49931799606686 31.0 30.0 34.0 23.0 34.0 4 32.81880993862193 37.0 35.0 37.0 25.0 37.0 5 32.8920817488971 37.0 35.0 37.0 26.0 37.0 6 32.991200111721355 37.0 35.0 37.0 26.0 37.0 7 32.883705394447354 37.0 35.0 37.0 25.0 37.0 8 32.82624444541107 37.0 35.0 37.0 25.0 37.0 9 34.1600435896438 39.0 35.0 39.0 24.0 39.0 10 34.157843045231125 39.0 35.0 39.0 25.0 39.0 11 34.15572262884014 39.0 35.0 39.0 25.0 39.0 12 34.08288259413325 39.0 35.0 39.0 24.0 39.0 13 34.01376416262783 39.0 35.0 39.0 23.0 39.0 14 34.98310306066149 39.0 35.0 41.0 22.0 41.0 15 35.05504749302314 39.0 35.0 41.0 23.0 41.0 16 35.04582498666441 39.0 35.0 41.0 23.0 41.0 17 34.92112655419745 39.0 35.0 41.0 22.0 41.0 18 34.914185178147484 39.0 34.0 41.0 22.0 41.0 19 35.058330452540176 39.0 35.0 41.0 23.0 41.0 20 35.04955483160524 39.0 35.0 41.0 23.0 41.0 21 35.02945093990169 39.0 35.0 41.0 23.0 41.0 22 34.97494557017956 39.0 34.0 41.0 23.0 41.0 23 34.953132433304866 39.0 34.0 41.0 23.0 41.0 24 34.971468471912836 39.0 34.0 41.0 24.0 41.0 25 34.879444873065765 39.0 34.0 41.0 23.0 41.0 26 34.851393655234304 39.0 34.0 41.0 23.0 41.0 27 34.79035945430528 39.0 34.0 41.0 23.0 41.0 28 34.696639888644945 39.0 34.0 41.0 23.0 41.0 29 34.698164610429004 39.0 34.0 41.0 23.0 41.0 30 34.53177488146776 39.0 34.0 41.0 22.0 41.0 31 34.526695222767344 39.0 34.0 41.0 22.0 41.0 32 34.38422714233713 39.0 34.0 41.0 20.0 41.0 33 34.343971282117025 39.0 34.0 41.0 20.0 41.0 34 34.247110698006644 39.0 34.0 41.0 19.0 41.0 35 34.18738647576356 39.0 33.0 41.0 19.0 41.0 36 34.102156359532145 39.0 33.0 41.0 18.0 41.0 37 33.9882678840021 38.0 33.0 41.0 16.0 41.0 38 33.90633308989677 38.0 33.0 41.0 16.0 41.0 39 33.80294642182596 38.0 33.0 40.0 15.0 41.0 40 33.752549101307004 38.0 33.0 40.0 15.0 41.0 41 33.655591905696184 38.0 33.0 40.0 15.0 41.0 42 33.50408630016873 38.0 33.0 40.0 9.0 41.0 43 33.41759977655729 38.0 33.0 40.0 9.0 41.0 44 33.265694446670224 38.0 33.0 40.0 8.0 41.0 45 33.12978312778588 38.0 33.0 40.0 2.0 41.0 46 32.96870703134837 38.0 32.0 40.0 2.0 41.0 47 32.79974084308415 38.0 32.0 40.0 2.0 41.0 48 32.60025915691585 38.0 32.0 40.0 2.0 41.0 49 32.455236137279904 38.0 32.0 40.0 2.0 41.0 50 32.25606168484047 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8741028591061131 1101 2 1.1210908054122974 1101 3 1.2617752337029842 1101 4 1.319240362671053 1101 5 1.3263730737562156 1101 6 1.4387059768398487 1101 7 1.688742057941461 1101 8 1.725666169944347 1101 9 2.0220490552368346 1101 10 1.9927475589960864 1101 11 2.0656326632668254 1101 12 1.995391678142724 1101 13 2.190353662684693 1101 14 2.531287922668426 1101 15 2.450751265120317 1101 16 2.470227088487981 1101 17 2.5797765747003325 1101 18 2.3551257441271645 1101 19 2.370628278740597 1101 20 2.3443955692737433 1101 21 2.431825748467787 1101 22 2.316467542965313 1101 23 2.3805605436042327 1101 24 2.406371710725651 1101 25 2.3252038026073834 1101 26 2.3893424073775833 1101 27 2.35569299465336 1101 28 2.4208267958170495 1101 29 2.452889875254513 1101 30 2.521243615024801 1101 31 2.6311133378509055 1101 32 2.529895010122388 1101 33 2.4985632731886795 1101 34 2.6782019232795733 1101 35 2.552182843558306 1101 36 2.6696103364629167 1101 37 2.695114720000788 1101 38 2.8925014414028425 1101 39 2.863079687628016 1101 40 2.79423163560633 1101 41 2.793186986265468 1101 42 2.7473954966132155 1101 43 2.7363601994690043 1101 44 2.8256137876245475 1101 45 2.8583659142330475 1101 46 2.869539659252119 1101 47 2.8562730230408206 1101 48 2.807270950693443 1101 49 2.7371485042895713 1101 50 2.79157113711706 1102 1 0.5148500762823751 1102 2 0.6140023152957781 1102 3 0.6011627766879677 1102 4 0.746799607908514 1102 5 0.6580915110363392 1102 6 0.8227782840417106 1102 7 0.9584474304276824 1102 8 0.9901333636545289 1102 9 1.0723800136675479 1102 10 1.2843822050337295 1102 11 1.0535955476322698 1102 12 1.089405754015111 1102 13 1.129325078967959 1102 14 1.3507828738782308 1102 15 1.1554989953811727 1102 16 1.2915702760637942 1102 17 1.4072352779921857 1102 18 1.359931196405583 1102 19 1.2739320153923828 1102 20 1.3895017419455726 1102 21 1.258204144587964 1102 22 1.3096442723150759 1102 23 1.359220940075268 1102 24 1.1466650743913362 1102 25 1.2727637269274084 1102 26 1.4151865918843143 1102 27 1.1996577427231614 1102 28 1.4023766885785989 1102 29 1.2714820239283213 1102 30 1.3304721583738441 1102 31 1.444374122530803 1102 32 1.4779902778601155 1102 33 1.5297903187834123 1102 34 1.4969683698103111 1102 35 1.6071566517212545 1102 36 1.5134856719957241 1102 37 1.506220926626746 1102 38 1.4865673916374078 1102 39 1.5777342892612083 1102 40 1.600245100304413 1102 41 1.579771898937338 1102 42 1.6029438840750885 1102 43 1.5080422681053918 1102 44 1.4329954150459017 1102 45 1.5382071598213685 1102 46 1.5156386128990746 1102 47 1.3851387476690746 1102 48 1.3972935301885485 1102 49 1.4466722599179462 1102 50 1.4605191439154268 1103 1 0.29495474909110797 1103 2 0.40731019691374826 1103 3 0.3218236273221571 1103 4 0.47783821142968463 1103 5 0.530906949289637 1103 6 0.3831668828301247 1103 7 0.40126842967043785 1103 8 0.5573101163521983 1103 9 0.5585798858831126 1103 10 0.5268831515851318 1103 11 0.46906042268748394 1103 12 0.5366704176738466 1103 13 0.5873737238218766 1103 14 0.7104126539627913 1103 15 0.5678126430747454 1103 16 0.4668885539712804 1103 17 0.6058830898808694 1103 18 0.4489089997753055 1103 19 0.4734396114673487 1103 20 0.5439582242450598 1103 21 0.5052826531384724 1103 22 0.3885489940289659 1103 23 0.5739991709707084 1103 24 0.3314013415081405 1103 25 0.524506564688501 1103 26 0.46115664930842826 1103 27 0.42442327405401414 1103 28 0.4784711386322371 1103 29 0.6775366005043253 1103 30 0.6481075941030028 1103 31 0.4912964501006911 1103 32 0.5235247244430639 1103 33 0.402624885262874 1103 34 0.5593660239404343 1103 35 0.4003249170080565 1103 36 0.4391001416114193 1103 37 0.5128682810155851 1103 38 0.5964131037595806 1103 39 0.589083243321916 1103 40 0.5335457644679806 1103 41 0.5297894495778337 1103 42 0.4486942626956463 1103 43 0.6113849749268709 1103 44 0.5922668859867741 1103 45 0.47008227814029624 1103 46 0.5806016185152814 1103 47 0.4343712522440697 1103 48 0.3756267714960515 1103 49 0.4770577429746723 1103 50 0.5015318355920897 1104 1 0.4062820775303706 1104 2 0.4539433778440518 1104 3 0.29006986554312064 1104 4 0.359691652303745 1104 5 0.46264689283611915 1104 6 0.32082619497728615 1104 7 0.3614011517817559 1104 8 0.33047108400412384 1104 9 0.4519638368780221 1104 10 0.25059228718090054 1104 11 0.4213918804976018 1104 12 0.4579866512797892 1104 13 0.40057033714747803 1104 14 0.5088297955473493 1104 15 0.4600079577548044 1104 16 0.5076114219387406 1104 17 0.5388368242917565 1104 18 0.44828315458244106 1104 19 0.5172531350898169 1104 20 0.4645318955482125 1104 21 0.41601116914079483 1104 22 0.43540990748121544 1104 23 0.4498695183852206 1104 24 0.48121882009658634 1104 25 0.45289659302257945 1104 26 0.44709290835065474 1104 27 0.5465459289792065 1104 28 0.47007086065769244 1104 29 0.526896735842513 1104 30 0.47526766558217304 1104 31 0.4519772059982543 1104 32 0.4544915848333062 1104 33 0.5403634845502268 1104 34 0.5217063093204715 1104 35 0.5451037716241487 1104 36 0.5794866764816646 1104 37 0.5189305274577265 1104 38 0.5524013622866377 1104 39 0.6444504192508234 1104 40 0.5823479383576924 1104 41 0.6170398493674796 1104 42 0.5264245039646696 1104 43 0.7010938126927684 1104 44 0.765016258129215 1104 45 0.6599930901694009 1104 46 0.7001501245362718 1104 47 0.720654174518522 1104 48 0.7256845263418228 1104 49 0.806917065317819 1104 50 0.8181058664376053 1105 1 0.5423674095546218 1105 2 0.5381094990590043 1105 3 0.4553475783861707 1105 4 0.634228213152781 1105 5 0.6198645826143547 1105 6 0.48760682209091044 1105 7 0.41867845163994133 1105 8 0.49675398643060475 1105 9 0.5795868751999009 1105 10 0.5005143655077617 1105 11 0.5800131344131714 1105 12 0.5765211004514796 1105 13 0.5954251690704311 1105 14 0.7679466313040493 1105 15 0.7922367513714619 1105 16 0.6887832080593341 1105 17 0.7375614413067311 1105 18 0.5799425355726697 1105 19 0.5903047036530964 1105 20 0.6820551162068114 1105 21 0.6019040346324047 1105 22 0.7045046134827402 1105 23 0.6236445896835505 1105 24 0.6725895792095287 1105 25 0.5573827385491015 1105 26 0.6533467070322203 1105 27 0.6310510094722943 1105 28 0.7440570996902167 1105 29 0.6726648097745951 1105 30 0.6565426979196687 1105 31 0.5623405937209185 1105 32 0.6265942622219924 1105 33 0.7257771317520607 1105 34 0.7512801167292693 1105 35 0.7488592066608533 1105 36 0.781075889005237 1105 37 0.7490390723484026 1105 38 0.6921850517220136 1105 39 0.7605166994259918 1105 40 0.7074804052020198 1105 41 0.7775857686094554 1105 42 0.8537004012327358 1105 43 0.8528569853516146 1105 44 0.8339798609156972 1105 45 0.9227293876431801 1105 46 0.9239908448442193 1105 47 0.8749459054705753 1105 48 0.9478528527401267 1105 49 1.019405571871026 1105 50 1.0608104497904165 1106 1 0.27416557300276523 1106 2 0.20639681664318488 1106 3 0.14477918527664713 1106 4 0.25374227753851386 1106 5 0.17592992861022339 1106 6 0.1462342732587203 1106 7 0.1745029023374869 1106 8 0.24176738047681567 1106 9 0.27006771954732045 1106 10 0.32958320529789376 1106 11 0.1708756804034266 1106 12 0.2949925224505989 1106 13 0.1934189932809005 1106 14 0.40450982864511786 1106 15 0.20329362572868348 1106 16 0.22162347226995394 1106 17 0.38272507232943553 1106 18 0.15222283370808043 1106 19 0.25587601516089364 1106 20 0.2774798100617346 1106 21 0.3326849005173429 1106 22 0.296145186610282 1106 23 0.3706103794288893 1106 24 0.20305968028296206 1106 25 0.2699260047592702 1106 26 0.2616851539345717 1106 27 0.37503953914756494 1106 28 0.27944379383315976 1106 29 0.2628563790445 1106 30 0.24734932918322983 1106 31 0.16618802075139882 1106 32 0.3825732350987323 1106 33 0.37422730615502786 1106 34 0.3899130351147946 1106 35 0.3872459250175808 1106 36 0.379289070989671 1106 37 0.21506219396405157 1106 38 0.45444487327916505 1106 39 0.40990189231427365 1106 40 0.4828480086872773 1106 41 0.49272492777940613 1106 42 0.3137789590342237 1106 43 0.3425921725687715 1106 44 0.42306707450804026 1106 45 0.3833144436598559 1106 46 0.3191463515049193 1106 47 0.34788086788185524 1106 48 0.33227551964638025 1106 49 0.36330599204391234 1106 50 0.5430274957851395 1107 1 0.3190732165246608 1107 2 0.2104913069582821 1107 3 0.16179673121900962 1107 4 0.17062055494445616 1107 5 0.07954630939580198 1107 6 0.22332956097910284 1107 7 0.09813379113518295 1107 8 0.14923026993930932 1107 9 0.16164131744820054 1107 10 0.01132150692317424 1107 11 0.027255128312191346 1107 12 -0.04815090552001067 1107 13 0.18151098639918217 1107 14 -0.08885755483279922 1107 15 0.09772677159379128 1107 16 0.023367583928113334 1107 17 0.09214940654413084 1107 18 0.15915118928333527 1107 19 -0.004797379132781998 1107 20 0.020659442110051884 1107 21 0.19944512671446546 1107 22 -0.22872075316602292 1107 23 -0.20564284924005705 1107 24 -0.18219546807112152 1107 25 -0.08492834831552187 1107 26 0.26060004028938266 1107 27 0.0843651772909908 1107 28 0.08903874344967022 1107 29 0.07639451603666458 1107 30 -0.006527459479379161 1107 31 0.01527583084774875 1107 32 -0.004939197929353156 1107 33 -0.07984879891314733 1107 34 0.0506527388915714 1107 35 0.1166290567261683 1107 36 0.08539769827551424 1107 37 -0.028825186680236925 1107 38 0.04644811721419018 1107 39 0.12562927421243586 1107 40 0.07640323282473105 1107 41 0.15283854775189099 1107 42 -0.019024348463545948 1107 43 -0.013321689038384932 1107 44 0.22536456696830243 1107 45 0.30171149221281723 1107 46 0.12104789581135122 1107 47 0.28930637900064227 1107 48 0.26447372401413105 1107 49 0.2623582226721126 1107 50 0.3550953561621668 1108 1 0.018510189880377226 1108 2 0.03362278212121339 1108 3 0.07140279351183665 1108 4 -0.10012108932274089 1108 5 0.0015789955176117587 1108 6 -0.16823256845982826 1108 7 -0.20421206887777998 1108 8 -0.06730612484086151 1108 9 -0.10597475226485642 1108 10 -0.15522601283535664 1108 11 -0.19487434742147514 1108 12 -0.22688000823338683 1108 13 -0.14628676931469897 1108 14 -0.2732071867577943 1108 15 -0.18432616183528694 1108 16 -0.24272993773952578 1108 17 -0.32438289399865994 1108 18 -0.26845686676508507 1108 19 -0.16065173223484663 1108 20 -0.23789847241116746 1108 21 -0.17813633212518454 1108 22 -0.34731355917691786 1108 23 -0.3957331540092639 1108 24 -0.020756391166777632 1108 25 -0.0528751465375521 1108 26 -0.3058594763667202 1108 27 -0.23194364229436815 1108 28 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0.12984417640565482 2211 44 0.11831383833024489 2211 45 0.1988610408518099 2211 46 0.14811826516109505 2211 47 0.16097404204586496 2211 48 0.12540186055431235 2211 49 0.25871808408852104 2211 50 0.3375918230617998 2212 1 -0.1603158721634408 2212 2 -0.3754535222394182 2212 3 -0.27779528508984797 2212 4 -0.2239623865343603 2212 5 -0.15129855014772886 2212 6 -0.267420204679361 2212 7 -0.1801908413891482 2212 8 -0.179846087034079 2212 9 -0.46891018621277425 2212 10 -0.43275721949277823 2212 11 -0.39938920505699826 2212 12 -0.441451147631156 2212 13 -0.33307849466397954 2212 14 -1.1892316915479029 2212 15 -0.6833702822386272 2212 16 -0.5944807771464014 2212 17 -0.37044343839484384 2212 18 -0.44258064380148454 2212 19 -0.46780396074629493 2212 20 -0.31533221683520196 2212 21 -0.591543279790109 2212 22 -0.5573270003970165 2212 23 -0.6319103853321195 2212 24 -0.4945583286302906 2212 25 -0.3060407948488333 2212 26 -0.6247398387977086 2212 27 -0.7075090035740175 2212 28 -0.5592230034136634 2212 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-0.7064014492625432 2216 9 -0.6884765622464784 2216 10 -0.8246968955995726 2216 11 -0.9701117262720089 2216 12 -0.9150450360619047 2216 13 -0.7942987677033457 2216 14 -0.8249260727948737 2216 15 -1.0302493099371546 2216 16 -0.7741680602282202 2216 17 -0.7519341669421422 2216 18 -0.6637647778608127 2216 19 -0.9794716573067461 2216 20 -0.9227621669054216 2216 21 -1.0114532651759802 2216 22 -0.846933014567945 2216 23 -1.0206641204305953 2216 24 -1.1730666278515898 2216 25 -1.2364337558934437 2216 26 -0.988310065218144 2216 27 -0.9922940637932385 2216 28 -0.9983094212095267 2216 29 -1.146395692868964 2216 30 -1.1601782889122845 2216 31 -1.2705534383420414 2216 32 -1.2767031376191227 2216 33 -1.195358873398085 2216 34 -1.2417162044943097 2216 35 -1.2608191184757942 2216 36 -1.2640655380687704 2216 37 -1.474969611668996 2216 38 -1.4564946835041255 2216 39 -1.4999293891616574 2216 40 -1.4880921949424533 2216 41 -1.5366028604173394 2216 42 -1.3600246680822465 2216 43 -1.4858976410539668 2216 44 -1.7130567343669938 2216 45 -1.6892025935613937 2216 46 -1.8547672130674933 2216 47 -1.8628175491333394 2216 48 -1.7529030518293993 2216 49 -1.721149856324704 2216 50 -1.7490327462394184 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 89876.0 3 4156.0 4 8266.0 5 6548.0 6 5519.0 7 4880.0 8 4574.0 9 4178.0 10 4058.0 11 4295.0 12 4478.0 13 4654.0 14 4894.0 15 5175.0 16 5490.0 17 5809.0 18 6213.0 19 7443.0 20 10553.0 21 15587.0 22 19842.0 23 21553.0 24 20940.0 25 20365.0 26 21498.0 27 23823.0 28 28080.0 29 31672.0 30 37009.0 31 45215.0 32 56011.0 33 69967.0 34 90917.0 35 119820.0 36 159150.0 37 220607.0 38 353531.0 39 637359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.45994883907221 17.832360432494063 13.111706247187385 23.59598448124634 2 15.589317190812768 20.777139902965217 38.65001938679077 24.983523519431248 3 20.490205065681522 25.393997451575352 27.614885366725638 26.50091211601749 4 12.59400331392191 16.425456237208973 36.91869432393472 34.0618461249344 5 13.93223079588391 38.80229673913641 32.79182684992181 14.473645615057867 6 31.66431186709035 36.49371259350478 17.39592152378439 14.446054015620478 7 28.834451666872052 32.76621827958812 20.4123395855256 17.986990468014223 8 26.56063237572002 33.971273891274 19.488325064723313 19.97976866828267 9 26.576558002985294 17.82903544145823 18.885342818841636 36.70906373671484 10 15.714804650585402 30.072280039229753 31.40739254533936 22.805522764845488 11 34.00322401400279 24.01253691605974 22.7576542847366 19.226584785200867 12 24.67382475436355 26.268749435930655 28.077802857707006 20.979622951998785 13 28.248953014612443 22.445983250066195 24.85915744589621 24.44590628942515 14 22.260667441170114 22.093688041251472 27.36029619259482 28.2853483249836 15 24.316041333683323 29.20601195556221 23.37651866745277 23.10142804330169 16 22.727172774725197 28.80811084937426 25.38566482229228 23.079051553608256 17 22.705097787341224 28.460649362370006 25.68908797630986 23.145164873978917 18 22.169960642709004 28.431027160699703 27.073060894080736 22.325951302510553 19 23.197761602614825 28.134917780497226 27.06662091153208 21.600699705355872 20 23.944125974063464 26.70919013727955 26.060203960538487 23.286479928118503 21 24.658499680609562 26.621490714765557 26.317359534652258 22.402650069972623 22 23.71664124152298 26.220143404225876 26.125331829056886 23.937883525194263 23 23.10569375355625 27.527765077346388 26.54534725931817 22.82119390977919 24 23.93400120879257 26.492398307690408 26.621021535111577 22.952578948405453 25 23.037371871013153 26.77964563811535 26.702062528412007 23.480919962459488 26 23.97060090439016 26.417989922300166 26.940662913954625 22.67074625935505 27 22.62789609677888 26.509901818063515 27.50455123014951 23.357650855008096 28 22.350984423567503 27.49332458249423 27.105861626134452 23.049829367803817 29 23.044147117035664 27.534058815469475 26.292502775892558 23.1292912916023 30 23.32917553749669 27.302604747585747 26.70588008867928 22.66233962623829 31 23.261454167296584 26.194573100058182 26.798422231690054 23.745550500955183 32 22.799749689402297 27.535631695258754 26.617856710926073 23.046761904412868 33 23.332601862638967 26.63232877515614 26.258820597420673 23.77624876478422 34 23.12792009649403 26.76706064730342 26.83879899199838 23.26622026420417 35 24.308914893646264 26.769969987579962 26.28544647311687 22.635668645656903 36 23.593978851671704 26.646976117076765 26.441640976867497 23.317404054384035 37 23.355044785884026 26.43285736734779 27.596406133817503 22.615691712950685 38 23.672643700065095 26.81643836118457 26.598569744755647 22.912348193994685 39 23.051261165592063 26.97709662079638 26.913142711590776 23.05849950202078 40 23.64064006098611 26.51035920967275 26.896974150537478 22.95202657880366 41 22.881934726435798 27.38078933108087 26.726303874922188 23.01097206756115 42 22.976930772162287 27.64757453388837 26.399388411702628 22.976106282246718 43 22.91572004397215 26.381183583730305 26.688118358373032 24.014978013924516 44 23.394653202952846 26.753222602440125 26.55819234710072 23.29393184750631 45 22.97906680430993 26.46538323787156 26.348456688629135 24.20709326918937 46 22.908018392657592 27.343781486434498 26.616227306867934 23.131972814039973 47 24.051713003547746 26.51177681351386 26.00590466611522 23.43060551682317 48 22.64666563162501 27.14071574504451 26.68076338443966 23.53185523889082 49 24.180967167241434 26.172045670005357 26.441705709666635 23.205281453086574 50 22.940425643000854 26.452445255792036 27.081946971636473 23.525182129570645 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5072.0 1 3759.0 2 2446.0 3 2235.0 4 2024.0 5 1834.5 6 1645.0 7 1502.5 8 1360.0 9 1480.0 10 1600.0 11 1972.0 12 2344.0 13 3503.5 14 4663.0 15 6360.5 16 8058.0 17 8507.5 18 8957.0 19 9313.5 20 9670.0 21 10493.0 22 11316.0 23 13408.0 24 15500.0 25 19585.5 26 23671.0 27 28469.5 28 33268.0 29 38587.0 30 43906.0 31 50197.5 32 56489.0 33 63364.0 34 70239.0 35 79352.0 36 88465.0 37 93496.5 38 98528.0 39 105195.5 40 111863.0 41 119368.5 42 126874.0 43 129043.0 44 131212.0 45 142355.5 46 153499.0 47 173036.0 48 192573.0 49 183681.5 50 174790.0 51 162364.5 52 149939.0 53 142254.0 54 134569.0 55 130484.5 56 126400.0 57 117692.0 58 108984.0 59 98021.5 60 87059.0 61 76370.0 62 65681.0 63 56752.0 64 47823.0 65 39872.5 66 31922.0 67 26368.5 68 20815.0 69 16759.0 70 12703.0 71 10031.0 72 7359.0 73 6121.5 74 4884.0 75 3646.5 76 2409.0 77 1890.0 78 1371.0 79 1098.5 80 826.0 81 665.0 82 504.0 83 403.5 84 303.0 85 231.5 86 160.0 87 133.0 88 106.0 89 85.0 90 64.0 91 48.0 92 32.0 93 24.0 94 16.0 95 14.0 96 12.0 97 10.0 98 8.0 99 16.0 100 24.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08566830204143305 2 0.09647413810865818 3 0.10370855378078346 4 0.10531111421448211 5 0.09748146180984019 6 0.0562727649433037 7 0.05238083246146415 8 0.05732587608544852 9 0.059844185338403535 10 0.04432224285200812 11 0.046336890254372126 12 0.055219653801158876 13 0.04903834927117841 14 0.050411972500062956 15 0.049816735767546326 16 0.052701344548203875 17 0.05293028175301797 18 0.054120755218051236 19 0.06282036900098673 20 0.0697800600273351 21 0.07889176077893593 22 0.08159321979574223 23 0.05618119006137807 24 0.07609872688020403 25 0.0837452295209947 26 0.08063168353552304 27 0.0745419538874682 28 0.097023587400212 29 0.0835162923161806 30 0.08054010865359741 31 0.05380024313131151 32 0.05114457155546805 33 0.05439547986382815 34 0.04908413671214123 35 0.05686800167582033 36 0.056410127266192156 37 0.05888264907818434 38 0.047527363719405406 39 0.05416654265901406 40 0.055540165887898604 41 0.041941295921941575 42 0.03832408808587893 43 0.03685888997506874 44 0.034523730485965 45 0.026694078081323076 46 0.02403840650547961 47 0.029624474302943446 48 0.023717894418739883 49 0.02358053209585143 50 0.02435891859221934 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2184005.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.984485898551036 #Duplication Level Percentage of deduplicated Percentage of total 1 74.4641155211826 39.454428787801746 2 12.891002840562797 13.660463164479616 3 5.251973009059397 8.34819269514235 4 2.4745336801866187 5.2444757953335 5 1.406744609575267 3.7267819964451707 6 0.9064303886868521 2.881604888843799 7 0.5771564540129901 2.1406236599236292 8 0.3838953669421846 1.6272398925013805 9 0.2794923443872985 1.3327882361947605 >10 1.2468537547682919 11.253433541159824 >50 0.07332342026482877 2.665158800051417 >100 0.04011167422441638 3.9901723273588665 >500 0.0020961293504147193 0.7826655370471735 >1k 0.0020961293504146127 2.1860769804355886 >5k 8.733872293394218E-5 0.23887657436512505 >10k+ 8.733872293394218E-5 0.46701712291608727 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 10092 0.4620868541967624 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5162 0.2363547702500681 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4650 0.21291160047710514 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4126 0.18891898141258834 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3767 0.17248129010693655 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3026 0.1385527963534882 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2797 0.1280674723730028 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2597 0.11890998418043915 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2448 0.11208765547697921 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2243 0.10270123007960147 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.157488192563662E-5 2 0.0 0.0 0.0 0.0 9.157488192563662E-5 3 0.0 0.0 0.0 0.0 9.157488192563662E-5 4 0.0 0.0 0.0 0.0 9.157488192563662E-5 5 0.0 0.0 0.0 0.0 9.157488192563662E-5 6 0.0 0.0 0.0 0.0 9.157488192563662E-5 7 0.0 0.0 0.0 0.0 9.157488192563662E-5 8 0.0 0.0 0.0 0.0 9.157488192563662E-5 9 0.0 0.0 0.0 0.0 9.157488192563662E-5 10 0.0 0.0 0.0 1.3736232288845494E-4 9.157488192563662E-5 11 0.0 0.0 0.0 3.2051208673972815E-4 9.157488192563662E-5 12 0.0 0.0 0.0 3.2051208673972815E-4 3.662995277025465E-4 13 0.0 0.0 0.0 3.2051208673972815E-4 3.662995277025465E-4 14 0.0 0.0 0.0 3.662995277025465E-4 3.662995277025465E-4 15 0.0 0.0 0.0 3.662995277025465E-4 5.036618505910014E-4 16 0.0 0.0 0.0 3.662995277025465E-4 5.036618505910014E-4 17 0.0 0.0 0.0 5.95236732516638E-4 5.494492915538197E-4 18 0.0 0.0 0.0 6.410241734794563E-4 6.410241734794563E-4 19 0.0 0.0 0.0 6.410241734794563E-4 6.410241734794563E-4 20 0.0 0.0 0.0 6.410241734794563E-4 6.868116144422746E-4 21 0.0 0.0 0.0 7.783864963679112E-4 6.868116144422746E-4 22 0.0 0.0 0.0 9.157488192563662E-4 7.783864963679112E-4 23 0.0 0.0 0.0 0.0012362609059960944 7.783864963679112E-4 24 0.0 0.0 0.0 0.0016025604336986409 7.783864963679112E-4 25 0.0 0.0 0.0 0.0018772850794755507 7.783864963679112E-4 26 0.0 0.0 0.0 0.002609884134880644 8.241739373307295E-4 27 0.0 0.0 0.0 0.0034798455131741916 8.241739373307295E-4 28 0.0 0.0 0.0 0.005311343151686924 8.241739373307295E-4 29 0.0 0.0 0.0 0.007646502640790658 8.241739373307295E-4 30 0.0 0.0 0.0 0.012042096973221216 8.241739373307295E-4 31 0.0 0.0 0.0 0.02248163351274379 8.241739373307295E-4 32 0.0 0.0 0.0 0.03420321839922528 9.157488192563662E-4 33 0.0 0.0 0.0 0.05109878411450523 9.157488192563662E-4 34 0.0 0.0 0.0 0.07170313254777347 0.001053111142144821 35 0.0 0.0 0.0 0.09487157767495953 0.001053111142144821 36 0.0 0.0 0.0 0.12184038040205952 0.001053111142144821 37 0.0 0.0 0.0 0.16071391777949226 0.001053111142144821 38 0.0 0.0 0.0 0.20503616063150037 0.001053111142144821 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAA 110 7.2759576E-12 25.995413 10 GTTCGCG 70 8.1283203E-4 18.856407 16 GCGTAAC 165 8.367351E-11 18.663801 11 TATCGCA 60 0.0074009513 18.33892 4 TCGCGTA 135 5.539914E-8 17.923584 9 AGTTCGC 105 3.782309E-5 16.758947 10 ATATCGA 585 0.0 15.416082 34 AATATCG 605 0.0 15.270032 33 TTATCGG 160 3.8411417E-7 15.120254 44 GAGTACT 2710 0.0 14.692143 12 TACCGCG 105 7.1654585E-4 14.665757 17 GTACATG 10540 0.0 14.385786 1 GTTATCG 185 1.2711826E-7 14.266445 43 GTACTTT 2800 0.0 14.063415 14 TACATGG 10505 0.0 13.99347 2 AGTACTT 2865 0.0 13.974061 13 TATCGAA 630 0.0 13.96579 35 TTGACGC 95 0.0062331622 13.897696 28 ACATGGG 10980 0.0 13.228376 3 GCGTGTA 100 0.0087710265 13.198275 9 >>END_MODULE