FastQCFastQC Report
Mon 6 Feb 2017
SRR2077120_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077120_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2184005
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT100050.4581033468329972No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53030.24281079942582548No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51020.23360752379229904No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46840.21446837346984093No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38970.17843365743210293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34800.15934029455060772No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30390.13914803308600485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28340.1297616076886271No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28200.12912058351514763No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27990.12815904725492844No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27020.12371766548153508No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC703.2143398E-522.0022541
AGTATCG550.004481495420.0025085
ATAACGC1101.2654345E-720.002053
TATACCG1001.1229349E-619.8024855
CCGGTTA851.4321356E-418.116629
TAACGCA1254.849353E-717.6026124
ATATCGA5850.015.41685434
TATACAG4800.015.1268995
TATAACG1351.9555073E-514.6681712
AATATCG6150.014.66481233
TATCGAA6150.014.30680635
TCGCACG1251.6708644E-414.08112122
TAGATTG2703.092282E-1113.853595
GTACATG101450.013.6416161
GTATAGG3101.8189894E-1213.4852541
TAAGCTT3600.013.4461315
GCACCGC1505.27531E-513.2007486
TACATGG102000.013.1797832
TAGGACA5350.013.16083054
ATAGGAC2351.2360033E-813.1077283
Produced by FastQC (version 0.11.5)