FastQCFastQC Report
Mon 6 Feb 2017
SRR2077119_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077119_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859861
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC36160.126439711580388No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31080.10867661050659456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA609.770414E-625.66281910
ACCGTCG956.880655E-720.839718
CGCACGA856.108581E-620.70966328
TAGATCG550.00448080120.0033025
GCGTAAC1002.4881387E-517.59736311
TATTCGC800.001988445916.5009929
TTAACGT953.3414303E-416.2149054
GTATAGC5450.015.7473721
CGCGATC1004.940428E-415.40254223
TCAACGT1756.40448E-815.08846928
TATCATA8850.014.9190222
TTATAGG10400.014.8116914
CTTATAG10750.014.7391213
TCATACT10200.014.6706284
TATTATA2102.5393092E-914.6703722
CGATCGT1057.1531464E-414.66934525
GTATCAA55800.014.5128781
TATAGGC10400.014.1754155
CGATGCG1402.7617687E-514.14147933
ATAGGCC10650.014.0450896
Produced by FastQC (version 0.11.5)