##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077119_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2859861 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.20450958980174 31.0 30.0 34.0 23.0 34.0 2 29.31057908059168 31.0 30.0 34.0 23.0 34.0 3 29.412081915869337 31.0 30.0 34.0 23.0 34.0 4 32.79534110224238 37.0 35.0 37.0 25.0 37.0 5 32.769941616043575 37.0 35.0 37.0 25.0 37.0 6 32.83812080377333 37.0 35.0 37.0 25.0 37.0 7 32.691172053466936 37.0 35.0 37.0 25.0 37.0 8 32.57652487306201 37.0 33.0 37.0 23.0 37.0 9 33.966870767495344 38.0 34.0 39.0 23.0 39.0 10 33.917926430690166 38.0 34.0 39.0 23.0 39.0 11 33.94262588286634 38.0 35.0 39.0 23.0 39.0 12 33.859274629081625 38.0 34.0 39.0 23.0 39.0 13 33.7897597120979 38.0 34.0 39.0 22.0 39.0 14 34.750705016782284 39.0 34.0 41.0 19.0 41.0 15 34.78944501148832 39.0 34.0 41.0 21.0 41.0 16 34.757347647315726 39.0 34.0 41.0 21.0 41.0 17 34.60702670514406 39.0 34.0 41.0 18.0 41.0 18 34.57342577139239 39.0 34.0 41.0 18.0 41.0 19 34.69112519804284 39.0 34.0 41.0 19.0 41.0 20 34.67435340388921 39.0 34.0 41.0 19.0 41.0 21 34.61836047276424 39.0 34.0 41.0 18.0 41.0 22 34.55651795664195 39.0 34.0 41.0 18.0 41.0 23 34.5181150412555 39.0 34.0 41.0 18.0 41.0 24 34.50128240498402 39.0 34.0 41.0 18.0 41.0 25 34.40739532445808 39.0 34.0 41.0 18.0 41.0 26 34.358927933910074 39.0 34.0 41.0 18.0 41.0 27 34.291112400218054 39.0 34.0 41.0 17.0 41.0 28 34.19157014973805 39.0 34.0 41.0 17.0 41.0 29 34.186317446896894 39.0 34.0 41.0 16.0 41.0 30 34.02829752914565 39.0 34.0 41.0 16.0 41.0 31 34.02173567176866 39.0 34.0 41.0 16.0 41.0 32 33.88355378111034 39.0 33.0 41.0 15.0 41.0 33 33.844578809949155 39.0 33.0 41.0 15.0 41.0 34 33.75370131625278 38.0 33.0 41.0 12.0 41.0 35 33.68685471077091 38.0 33.0 41.0 9.0 41.0 36 33.604545815338575 38.0 33.0 41.0 9.0 41.0 37 33.515273994085724 38.0 33.0 40.0 9.0 41.0 38 33.43364869831086 38.0 33.0 40.0 8.0 41.0 39 33.32535392454388 38.0 33.0 40.0 8.0 41.0 40 33.26579753351649 38.0 33.0 40.0 7.0 41.0 41 33.149463208176904 38.0 33.0 40.0 2.0 41.0 42 33.00343233464843 38.0 32.0 40.0 2.0 41.0 43 32.939304742433286 38.0 32.0 40.0 2.0 41.0 44 32.80262712068873 38.0 32.0 40.0 2.0 41.0 45 32.673911074699085 38.0 32.0 40.0 2.0 41.0 46 32.54050354195536 38.0 32.0 40.0 2.0 41.0 47 32.39706195510901 38.0 32.0 40.0 2.0 41.0 48 32.212802300531386 38.0 32.0 40.0 2.0 41.0 49 32.0481803136586 38.0 31.0 40.0 2.0 41.0 50 31.84022125550857 38.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.25288622247889236 1101 2 0.5114654650073476 1101 3 0.5805205341396729 1101 4 0.5469133478452122 1101 5 0.679889768415272 1101 6 0.6274265003404906 1101 7 0.7748283109754439 1101 8 0.8584281502720401 1101 9 0.7644661794683643 1101 10 0.9453680388332799 1101 11 0.8160126781972608 1101 12 0.8758834839972067 1101 13 0.9197470294821883 1101 14 1.03875472870741 1101 15 1.1104692484957752 1101 16 1.0995609495193648 1101 17 1.276775398150562 1101 18 1.3263799500456557 1101 19 1.4519875694607904 1101 20 1.3501949886743958 1101 21 1.4250469931077703 1101 22 1.3235931588443393 1101 23 1.3209880012055848 1101 24 1.4337550599545281 1101 25 1.3876877980011102 1101 26 1.3631040291978636 1101 27 1.3163674251029889 1101 28 1.2013301286488698 1101 29 1.3145437073565063 1101 30 1.43479944716168 1101 31 1.4046135728781621 1101 32 1.3233888566286893 1101 33 1.3850427479618475 1101 34 1.4107398219614282 1101 35 1.2772159949902075 1101 36 1.3221755450303263 1101 37 1.307950226074702 1101 38 1.3442051115867883 1101 39 1.321246442437733 1101 40 1.36864330910673 1101 41 1.4386916361762232 1101 42 1.4949525632139569 1101 43 1.4470695385997558 1101 44 1.4659175690546462 1101 45 1.4423277584834793 1101 46 1.5434242863017005 1101 47 1.5185873354172017 1101 48 1.5287189718834782 1101 49 1.474440771856976 1101 50 1.4992598405208497 1102 1 0.4707162405620764 1102 2 0.5941565713693393 1102 3 0.6281302661797845 1102 4 0.6613207126323246 1102 5 0.7290908205277162 1102 6 0.6732279141165876 1102 7 0.7846902212171045 1102 8 0.6783706128727331 1102 9 0.8317619217008172 1102 10 1.0133706691143942 1102 11 0.7498150783287798 1102 12 0.825875264368733 1102 13 0.8553922703173029 1102 14 1.16512165292243 1102 15 1.0862920333059023 1102 16 0.9833814328335251 1102 17 1.2302523009945503 1102 18 1.1619051843050698 1102 19 1.0810899860315644 1102 20 1.1381729600700865 1102 21 1.0914648790193269 1102 22 1.1051368050715311 1102 23 1.0676245292345143 1102 24 1.0675903385999348 1102 25 1.0766652762190958 1102 26 1.1766614844008885 1102 27 1.0925880399312007 1102 28 1.1186932718348004 1102 29 1.0188425730477775 1102 30 1.0384900603459641 1102 31 1.1085639263221907 1102 32 1.200676379232668 1102 33 1.2360028005674693 1102 34 1.2175538939653734 1102 35 1.1824354590704331 1102 36 1.1995764985072341 1102 37 1.2004194024679293 1102 38 1.154512525691672 1102 39 1.3460450615401527 1102 40 1.3031493094355184 1102 41 1.2862816411080047 1102 42 1.149731455208041 1102 43 1.0930073982084991 1102 44 0.9709512695313833 1102 45 1.252339265963343 1102 46 1.255117529767098 1102 47 1.2236900807731104 1102 48 1.1643707884213725 1102 49 1.3163427938855818 1102 50 1.381702714102964 1103 1 0.31473679793893794 1103 2 0.4135607070434162 1103 3 0.3223907940572701 1103 4 0.35513944127840347 1103 5 0.48896855944672524 1103 6 0.39013036452364247 1103 7 0.42570967812900307 1103 8 0.4612646628718693 1103 9 0.5632124036583761 1103 10 0.5335065275634889 1103 11 0.5230281479346246 1103 12 0.49746367895018295 1103 13 0.5835078248997903 1103 14 0.6913793230479754 1103 15 0.6634278283774293 1103 16 0.5059001724266352 1103 17 0.664329264402653 1103 18 0.6222302535882704 1103 19 0.5667287454445713 1103 20 0.5070112493368129 1103 21 0.3515530314481268 1103 22 0.4027533477678418 1103 23 0.47319230577674887 1103 24 0.3992836142404812 1103 25 0.5589322340384442 1103 26 0.5615342971547648 1103 27 0.5282765219233312 1103 28 0.5732871656946656 1103 29 0.6241645598767676 1103 30 0.7014366561065799 1103 31 0.5872501670532202 1103 32 0.7387539648932062 1103 33 0.6780535832803807 1103 34 0.7365297125442183 1103 35 0.688971990474009 1103 36 0.7280043316283482 1103 37 0.6819688110400364 1103 38 0.7325723656345176 1103 39 0.7216043685353029 1103 40 0.7055265442148198 1103 41 0.6831326980729102 1103 42 0.646743375951516 1103 43 0.6304270095311892 1103 44 0.6586449600068889 1103 45 0.7017559043021535 1103 46 0.613539082747856 1103 47 0.6555030823921228 1103 48 0.5476234401962259 1103 49 0.5222447430337311 1103 50 0.5902776984026161 1104 1 0.607710092918115 1104 2 0.6552652690850493 1104 3 0.6738067582816534 1104 4 0.589616960950238 1104 5 0.6256934794814484 1104 6 0.6094517754623681 1104 7 0.6410852785336942 1104 8 0.6469251627434716 1104 9 0.6950432336448884 1104 10 0.6517498352459583 1104 11 0.7336027193867736 1104 12 0.7377445166566545 1104 13 0.7039073993284859 1104 14 0.8842645563301588 1104 15 0.7387665577150031 1104 16 0.8400068732471624 1104 17 0.9526992978062623 1104 18 0.749719336728667 1104 19 0.8736481541341661 1104 20 0.8616349602599342 1104 21 0.7552539718370213 1104 22 0.7804670524848873 1104 23 0.8233668530550986 1104 24 0.8096795324824413 1104 25 0.8521768819211601 1104 26 0.7458070121434588 1104 27 0.8097791082588301 1104 28 0.6885814305009674 1104 29 0.8217661937364724 1104 30 0.8513219409889814 1104 31 0.7078889697353858 1104 32 0.7876840233058573 1104 33 0.8079361536298464 1104 34 0.9369203683669411 1104 35 0.7954382006627867 1104 36 0.866714354258157 1104 37 0.8939432141188632 1104 38 0.9080528650684201 1104 39 1.0708735026294178 1104 40 0.9557811822613687 1104 41 0.9389886704167978 1104 42 0.9300421317838996 1104 43 0.9994094351390359 1104 44 0.9940341370918162 1104 45 1.0958987574007466 1104 46 1.0801353696603329 1104 47 1.0930085912676617 1104 48 1.0343477277768969 1104 49 1.1149058868037827 1104 50 1.1756695358101226 1105 1 0.3123297169604733 1105 2 0.32021792038656827 1105 3 0.23511474297762547 1105 4 0.277005345075402 1105 5 0.26101903088294875 1105 6 0.1888293521133093 1105 7 0.23845972852416253 1105 8 0.288663584043924 1105 9 0.34935093562624076 1105 10 0.28303885550300834 1105 11 0.3624479621733556 1105 12 0.20132050616980735 1105 13 0.3616241404017373 1105 14 0.4352917607721878 1105 15 0.3659099449049137 1105 16 0.3919934354230179 1105 17 0.48688237496047293 1105 18 0.3480706641193976 1105 19 0.18623922640015422 1105 20 0.4087361754527947 1105 21 0.4782923331866229 1105 22 0.4422222446660271 1105 23 0.46490996607423085 1105 24 0.4252322443588099 1105 25 0.40542658804675824 1105 26 0.5148422257129965 1105 27 0.5210697412851744 1105 28 0.5680556773113068 1105 29 0.4720801317590926 1105 30 0.5549801913358934 1105 31 0.41314981356892133 1105 32 0.49214371439333604 1105 33 0.5443032372005803 1105 34 0.5285082885329402 1105 35 0.5245436944941844 1105 36 0.6222172334833331 1105 37 0.5210213225516753 1105 38 0.6108147804815545 1105 39 0.7326471744587053 1105 40 0.6095955016367256 1105 41 0.5959016187942581 1105 42 0.7187079155873732 1105 43 0.5556955154520651 1105 44 0.6953580958836412 1105 45 0.6148769720555407 1105 46 0.6216240517158269 1105 47 0.5403416420463714 1105 48 0.6799526674588492 1105 49 0.666931904228413 1105 50 0.7114116712798619 1106 1 0.40460906775083316 1106 2 0.41653630713263823 1106 3 0.31037348241597584 1106 4 0.3440646111893457 1106 5 0.3995712131459186 1106 6 0.358407185957077 1106 7 0.46677809528254244 1106 8 0.4251090639352384 1106 9 0.49434710108337754 1106 10 0.5420186220134937 1106 11 0.4968252397883077 1106 12 0.5138542383685731 1106 13 0.6004029687757821 1106 14 0.6288804241359642 1106 15 0.5435875515790869 1106 16 0.443258144517678 1106 17 0.6865873661956385 1106 18 0.3378598106617545 1106 19 0.538186205725026 1106 20 0.5423563479798617 1106 21 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0.3224824978873002 1107 9 0.278786252397353 1107 10 0.1946616085023578 1107 11 0.21152111684630626 1107 12 0.14813721703608707 1107 13 0.22199156327141623 1107 14 0.03715137153545811 1107 15 0.20996786168117865 1107 16 0.18598956251081233 1107 17 0.3450870310450824 1107 18 0.3674654060873763 1107 19 0.3062449932829665 1107 20 0.3345450422905074 1107 21 0.3787199043654894 1107 22 0.08636044276587995 1107 23 0.04451660616153674 1107 24 0.1091199127249709 1107 25 0.11786784837531172 1107 26 0.37578694048329453 1107 27 0.3215931253681603 1107 28 0.23445186076782676 1107 29 0.3134346713104108 1107 30 0.31820934854683003 1107 31 0.220325032316957 1107 32 0.1857254106230144 1107 33 0.19958739895839273 1107 34 0.3388832914639792 1107 35 0.34436733095954963 1107 36 0.20564612821288364 1107 37 0.3324594377932186 1107 38 0.26388263909448284 1107 39 0.34458856862816134 1107 40 0.2933222358978824 1107 41 0.3414230097738269 1107 42 0.4264785580213726 1107 43 0.3692275384833792 1107 44 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6 -0.4519409929675291 2214 7 -0.45751181894832627 2214 8 -0.547799554282804 2214 9 -0.6610942576162344 2214 10 -0.580791592530808 2214 11 -0.7423199845963637 2214 12 -0.7148067427525007 2214 13 -0.6024786795464934 2214 14 -0.5792251229155667 2214 15 -0.46303343634519933 2214 16 -0.5449698604389539 2214 17 -0.3806308469880264 2214 18 -0.5495348002185381 2214 19 -0.6889440515823537 2214 20 -0.5060072353716976 2214 21 -0.6318802184662786 2214 22 -0.6772605723067855 2214 23 -0.6924804592889018 2214 24 -0.7978761753941797 2214 25 -0.8504382849205143 2214 26 -0.8179832643429563 2214 27 -0.845026921979084 2214 28 -0.7760764383487739 2214 29 -0.6979001684702624 2214 30 -0.7410549545291545 2214 31 -0.8073957932204223 2214 32 -0.7916639550783984 2214 33 -0.8161966776270191 2214 34 -0.9504120579636819 2214 35 -0.8816399569884226 2214 36 -0.8255446199760854 2214 37 -0.8035548941664743 2214 38 -0.7890279090068262 2214 39 -0.8469466408111117 2214 40 -0.7671511731561509 2214 41 -0.7887873491094481 2214 42 -0.7280346107025863 2214 43 -0.7912391993372339 2214 44 -0.5969736908626331 2214 45 -0.5220070393085692 2214 46 -0.5865947033827155 2214 47 -0.7077187418182405 2214 48 -0.6385423293926955 2214 49 -0.7373996182362035 2214 50 -0.6674808537291383 2215 1 -0.32738034877077027 2215 2 -0.5326021455286423 2215 3 -0.409227966159861 2215 4 -0.4238329378040149 2215 5 -0.38420050365871816 2215 6 -0.5002085133621534 2215 7 -0.3845794801480622 2215 8 -0.340614562389959 2215 9 -0.34588466776413895 2215 10 -0.39184052592694485 2215 11 -0.4313076491481098 2215 12 -0.5135092355771036 2215 13 -0.4242373622479789 2215 14 -0.46515669070181787 2215 15 -0.4444149093000931 2215 16 -0.45490670764083774 2215 17 -0.3070985863495963 2215 18 -0.3396385857912918 2215 19 -0.45375710331581587 2215 20 -0.5449537886597327 2215 21 -0.5403486215051245 2215 22 -0.458708749224634 2215 23 -0.741562316354127 2215 24 -0.7705633726043573 2215 25 -0.808122392643142 2215 26 -0.6372259997796235 2215 27 -0.6140422716498506 2215 28 -0.6151905662688222 2215 29 -0.706733335366998 2215 30 -0.5982827388635243 2215 31 -0.554186748069931 2215 32 -0.7244031343066979 2215 33 -0.6795628301377761 2215 34 -0.7247641126345528 2215 35 -0.6560380500672167 2215 36 -0.6183150122103882 2215 37 -0.5223361333600565 2215 38 -0.6134506119424472 2215 39 -0.6839555461393303 2215 40 -0.7218345077956272 2215 41 -0.7312182598138222 2215 42 -0.6822183768874766 2215 43 -0.7573079874433901 2215 44 -0.7986680853243726 2215 45 -0.7558411567173735 2215 46 -0.7550025529829973 2215 47 -0.6633983682967397 2215 48 -0.7597896495305925 2215 49 -0.8883556781968309 2215 50 -0.8149312699758333 2216 1 -0.5916817843645603 2216 2 -0.6522486651738504 2216 3 -0.6775905164863474 2216 4 -0.558067599351304 2216 5 -0.6093590067616432 2216 6 -0.658571633262433 2216 7 -0.687478244744284 2216 8 -0.7728223357688648 2216 9 -0.727574414668041 2216 10 -0.7720438905546985 2216 11 -0.9328130469757667 2216 12 -0.8990134753308894 2216 13 -0.839721047351162 2216 14 -0.9377714397349308 2216 15 -1.0959464914528994 2216 16 -1.0933195233012256 2216 17 -0.9468150832243509 2216 18 -0.9642569579565219 2216 19 -1.0364524336498704 2216 20 -1.0066212489801671 2216 21 -1.1534311156098624 2216 22 -1.039055569672655 2216 23 -1.0166937780929644 2216 24 -1.1718130246554708 2216 25 -1.3910080570420504 2216 26 -1.2461968495640932 2216 27 -1.2873964756668954 2216 28 -1.3266580508363148 2216 29 -1.426279840353267 2216 30 -1.4415128632823695 2216 31 -1.678872701326675 2216 32 -1.5111705958948392 2216 33 -1.580148946571164 2216 34 -1.6362078411538974 2216 35 -1.471512522001781 2216 36 -1.5765218653437287 2216 37 -1.6245600779924843 2216 38 -1.626539114247258 2216 39 -1.6644134066951715 2216 40 -1.7197811406949626 2216 41 -1.6722345633727294 2216 42 -1.6136250866810578 2216 43 -1.845904254074636 2216 44 -1.8902726479140135 2216 45 -1.9196599544024373 2216 46 -2.160479594248887 2216 47 -2.0472109381655024 2216 48 -2.056956430558394 2216 49 -2.0250187506564394 2216 50 -2.2139643604279016 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 138754.0 3 6829.0 4 12953.0 5 10114.0 6 8400.0 7 7383.0 8 6784.0 9 5980.0 10 6035.0 11 6461.0 12 6419.0 13 6591.0 14 6981.0 15 7359.0 16 8022.0 17 8709.0 18 9212.0 19 10259.0 20 12604.0 21 16032.0 22 19618.0 23 22359.0 24 24102.0 25 26209.0 26 29592.0 27 33427.0 28 38382.0 29 44495.0 30 51445.0 31 62242.0 32 76123.0 33 95075.0 34 120225.0 35 156104.0 36 206469.0 37 284143.0 38 450393.0 39 817577.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.32520254072687 16.008819051951914 12.837774939194036 25.828203468127175 2 16.520905840419545 18.815956365550512 37.55732901394096 27.10580878008898 3 20.408702579496197 22.221868314647015 26.97235414029861 30.39707496555818 4 13.70159333306965 15.326358337355664 34.59045904275408 36.381589286820606 5 15.633573839802741 35.89470681602218 32.73447630780861 15.737243036366468 6 35.12999108528596 33.50525996117831 16.571431770249486 14.793317183286241 7 30.85324148499039 30.1327171237801 19.85862398914053 19.15541740208898 8 28.124501426096305 33.108526068592575 18.89836509744758 19.868607407863543 9 26.97592253336823 16.050093278490525 17.532647471684065 39.44133671645718 10 15.765460689722726 28.54478160179672 31.842863839582442 23.846893868898107 11 36.39494359577873 21.741250751274983 21.934885536828393 19.9289201161179 12 25.087948555124633 23.521685813298905 27.983507131223966 23.40685850035249 13 29.060002190052476 20.536429913597882 24.460087343210958 25.943480553138688 14 23.213432948087092 19.849773207922766 25.33973330721099 31.597060536779146 15 24.811841734697985 27.291616011251186 22.22515319072267 25.67138906332816 16 24.86441136936295 26.24398326544864 23.646895088581307 25.2447102766071 17 24.38683747239614 26.38981407104093 24.852954347625296 24.37039410893763 18 24.22735903152979 26.221144806966144 25.58688246536992 23.964613696134148 19 24.94695658137266 25.564688478381658 25.81860410435698 23.6697508358887 20 25.496486906378152 25.214144750654334 24.79964169244335 24.489726650524165 21 25.80165959072947 24.986562018384838 24.819812064227953 24.391966326657734 22 25.24715608646141 24.755328597735225 24.89303609511231 25.10447922069105 23 25.257166928548163 25.44980814600147 24.67665420061767 24.6163707248327 24 24.974043330054414 25.28552336940147 25.174103029520438 24.566330271023677 25 24.872150128103595 25.0377521520039 24.85860659825376 25.231491121638744 26 25.025984015543518 25.068888262084055 25.373347434109085 24.531780288263345 27 24.664804303102645 25.19908778479168 25.276528795073173 24.859579117032506 28 24.03198449915195 25.2233881925034 25.69081464152539 25.05381266681927 29 24.54889021796684 25.556640826638265 24.918660079448376 24.975808875946516 30 24.87886374437188 25.27924505323836 25.135169373786976 24.706721828602785 31 25.14652229951845 25.104014039271334 25.113320374436547 24.636143286773667 32 25.0372505768198 25.188561912575285 24.899793411921934 24.874394098682977 33 24.824875605876876 25.50843976777431 24.582909706415176 25.08377491993364 34 24.70842181492508 25.333314674941665 25.00113699574239 24.95712651439087 35 25.073279617105694 25.205284389289872 25.204969509101787 24.516466484502647 36 25.18080150581913 25.331698297381784 24.796194867829733 24.691305328969353 37 25.05374978438858 24.737566349713504 25.40103724400845 24.807646621889468 38 25.3204638202373 25.12350289581537 24.763865541808716 24.79216774213862 39 24.985402208088768 25.155327852936825 24.841331010747965 25.017938928226446 40 24.988043303707784 24.87143271485055 25.40606410048733 24.734459880954336 41 24.716060447369866 25.29200288670858 25.220569792616644 24.771366873304906 42 24.676746151456726 25.36288663832788 25.242589053094644 24.717778157120748 43 24.823354712552405 24.581601045232176 25.694941223180507 24.90010301903491 44 25.319041540899896 24.907959536826155 25.076911721958574 24.69608720031538 45 24.679575518884093 25.048513126875644 25.2237479975656 25.048163356674664 46 24.569420957184303 25.588092818931734 24.84817854475948 24.994307679124482 47 25.178193794539705 24.795712855626824 24.945802083690396 25.080291266143075 48 24.646055537776284 25.22881920580875 25.41961117610746 24.705514080307506 49 25.425581922421536 24.52240950438313 25.037703592403883 25.01430498079145 50 24.89021814367494 24.969544388245303 24.901270653941356 25.238966814138397 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1596.0 1 1194.5 2 793.0 3 761.5 4 730.0 5 743.5 6 757.0 7 803.5 8 850.0 9 972.0 10 1094.0 11 1279.5 12 1465.0 13 2004.0 14 2543.0 15 3303.0 16 4063.0 17 4323.0 18 4583.0 19 4531.5 20 4480.0 21 4599.0 22 4718.0 23 5442.5 24 6167.0 25 7306.0 26 8445.0 27 10319.5 28 12194.0 29 15399.0 30 18604.0 31 23569.5 32 28535.0 33 35915.5 34 43296.0 35 56023.0 36 68750.0 37 79260.0 38 89770.0 39 106520.0 40 123270.0 41 144332.0 42 165394.0 43 175034.0 44 184674.0 45 197761.0 46 210848.0 47 228908.5 48 246969.0 49 253316.5 50 259664.0 51 256365.0 52 253066.0 53 244661.0 54 236256.0 55 234612.0 56 232968.0 57 212291.5 58 191615.0 59 171453.5 60 151292.0 61 130604.0 62 109916.0 63 91796.0 64 73676.0 65 60261.0 66 46846.0 67 37832.0 68 28818.0 69 22832.5 70 16847.0 71 13476.5 72 10106.0 73 8206.0 74 6306.0 75 4907.5 76 3509.0 77 2707.0 78 1905.0 79 1503.5 80 1102.0 81 840.5 82 579.0 83 464.5 84 350.0 85 260.5 86 171.0 87 134.0 88 97.0 89 80.5 90 64.0 91 52.5 92 41.0 93 30.0 94 19.0 95 16.0 96 13.0 97 13.5 98 14.0 99 23.5 100 33.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08430479663172441 2 0.09332621410621006 3 0.1003195609856563 4 0.10119372934558708 5 0.0942703159349353 6 0.058219612771389935 7 0.05335923669017481 8 0.05975814908486811 9 0.06129668539834629 10 0.04619105613874241 11 0.048498860608959665 12 0.057904912161814856 13 0.05182070037669662 14 0.05300956934620248 15 0.05231023465825786 16 0.055352340550816975 17 0.05472293933166682 18 0.05524744034762529 19 0.06381429027494694 20 0.06926910084091499 21 0.0800388550352622 22 0.08234665950547947 23 0.05937351500649857 24 0.07741634995546987 25 0.08419989642853272 26 0.08164732481753484 27 0.07699674914270309 28 0.09535428470124947 29 0.08419989642853272 30 0.08164732481753484 31 0.05570200789478929 32 0.05300956934620248 33 0.05619154217635053 34 0.05073673161038247 35 0.057030743801884075 36 0.056506242785925614 37 0.059793115819265344 38 0.04986256325045168 39 0.05426837178450282 40 0.05699577706748685 41 0.043428684121361145 42 0.03905784232170724 43 0.04077121230717157 44 0.03720460539865399 45 0.029407023628071436 46 0.026085183860334468 47 0.03129522728552192 48 0.025385849172389844 49 0.025071148562814765 50 0.027343986298634795 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2859861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.89239096558068 #Duplication Level Percentage of deduplicated Percentage of total 1 76.88988430735661 39.13110053468259 2 12.158190966574441 12.375188162101953 3 4.603729189156231 7.028843573825839 4 2.128333018873621 4.3326382440588365 5 1.1742652041528425 2.988058193351194 6 0.7190319514827581 2.1955953114962976 7 0.4550725813116466 1.6211812208079828 8 0.31070282120620135 1.264992756074793 9 0.23519141725242285 1.0772508202703388 >10 1.1265811541113386 10.411215396375756 >50 0.10453922789741081 3.713108787042575 >100 0.0816991133838971 8.407342271679369 >500 0.010209347523945901 3.5261680651747356 >1k 0.0025696997168905837 1.9273166630578025 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC 3616 0.126439711580388 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3108 0.10867661050659456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 6.993346879446239E-5 7 0.0 0.0 0.0 0.0 6.993346879446239E-5 8 0.0 0.0 0.0 0.0 6.993346879446239E-5 9 0.0 0.0 0.0 0.0 6.993346879446239E-5 10 0.0 0.0 0.0 6.993346879446239E-5 6.993346879446239E-5 11 0.0 0.0 0.0 1.0490020319169359E-4 6.993346879446239E-5 12 0.0 0.0 0.0 1.0490020319169359E-4 2.7973387517784957E-4 13 0.0 0.0 0.0 1.0490020319169359E-4 3.8463407836954313E-4 14 0.0 0.0 0.0 1.0490020319169359E-4 3.8463407836954313E-4 15 0.0 0.0 0.0 1.0490020319169359E-4 4.545675471640055E-4 16 0.0 0.0 0.0 1.3986693758892479E-4 4.545675471640055E-4 17 0.0 0.0 0.0 1.3986693758892479E-4 4.895342815612367E-4 18 0.0 0.0 0.0 1.3986693758892479E-4 4.895342815612367E-4 19 0.0 0.0 0.0 1.3986693758892479E-4 5.245010159584679E-4 20 0.0 0.0 0.0 1.3986693758892479E-4 6.643679535473926E-4 21 0.0 0.0 0.0 1.7483367198615598E-4 6.643679535473926E-4 22 0.0 0.0 0.0 2.0980040638338718E-4 6.643679535473926E-4 23 0.0 0.0 0.0 3.8463407836954313E-4 6.643679535473926E-4 24 0.0 0.0 0.0 4.895342815612367E-4 6.643679535473926E-4 25 0.0 0.0 0.0 5.594677503556991E-4 6.993346879446239E-4 26 0.0 0.0 0.0 7.692681567390863E-4 6.993346879446239E-4 27 0.0 0.0 0.0 0.0010140352975197046 6.993346879446239E-4 28 0.0 0.0 0.0 0.001783303454258791 6.993346879446239E-4 29 0.0 0.0 0.0 0.003286873033339732 6.993346879446239E-4 30 0.0 0.0 0.0 0.005419843831570835 6.993346879446239E-4 31 0.0 0.0 0.0 0.011888689695058605 7.343014223418551E-4 32 0.0 0.0 0.0 0.01874216963691592 7.692681567390863E-4 33 0.0 0.0 0.0 0.029372056893674205 8.042348911363174E-4 34 0.0 0.0 0.0 0.04150551372951343 8.741683599307799E-4 35 0.0 0.0 0.0 0.058394446443376095 8.741683599307799E-4 36 0.0 0.0 0.0 0.07867515239377018 8.741683599307799E-4 37 0.0 0.0 0.0 0.1045855025821185 8.741683599307799E-4 38 0.0 0.0 0.0 0.133398091725437 8.741683599307799E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAA 60 9.770414E-6 25.662819 10 ACCGTCG 95 6.880655E-7 20.83971 8 CGCACGA 85 6.108581E-6 20.709663 28 TAGATCG 55 0.004480801 20.003302 5 GCGTAAC 100 2.4881387E-5 17.597363 11 TATTCGC 80 0.0019884459 16.50099 29 TTAACGT 95 3.3414303E-4 16.214905 4 GTATAGC 545 0.0 15.747372 1 CGCGATC 100 4.940428E-4 15.402542 23 TCAACGT 175 6.40448E-8 15.088469 28 TATCATA 885 0.0 14.919022 2 TTATAGG 1040 0.0 14.811691 4 CTTATAG 1075 0.0 14.739121 3 TCATACT 1020 0.0 14.670628 4 TATTATA 210 2.5393092E-9 14.670372 2 CGATCGT 105 7.1531464E-4 14.669345 25 GTATCAA 5580 0.0 14.512878 1 TATAGGC 1040 0.0 14.175415 5 CGATGCG 140 2.7617687E-5 14.141479 33 ATAGGCC 1065 0.0 14.045089 6 >>END_MODULE