FastQCFastQC Report
Mon 6 Feb 2017
SRR2077119_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077119_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859861
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC37690.13178962194316438No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29870.10444563564452958No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATC1101.2663986E-720.0011323
CGATCGT1206.4092437E-616.5009325
CTTATAG11950.016.2014683
GTATCAA57700.016.1667251
CCGCGAT1501.8385981E-716.13452722
TCTTATA13500.016.1339632
TTATAGG11250.015.4503024
CGGTTTA2455.456968E-1215.26643622
AATATCG7250.015.17061733
TATAGGC11350.015.1203255
ATAGGCC11400.015.0532186
GGTATCA36650.014.8870861
ATATCGA7250.014.86720534
TATCATA8550.014.6672382
TCGTATG1554.587162E-614.1888940
ACCGTCG1100.001017757914.0015268
ATAGTAC3000.013.9338763
TACGTAC950.00624097413.8952783
TCATACT10800.013.8531184
CCCCGCG1606.3434454E-613.75125720
Produced by FastQC (version 0.11.5)