FastQCFastQC Report
Mon 6 Feb 2017
SRR2077118_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077118_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617956
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCGT551.5920229E-424.0050823
GTATCAA32250.022.1782021
GGTATCA17000.019.6773851
AACGTCG808.977746E-519.25444222
TATCAAC41900.016.9121172
TCAACGC42000.016.5047554
CAACGCA42700.016.1817195
ATCAACG43850.015.9091063
GTATAAT2152.1464075E-1015.3541411
AACGCAG45550.015.2123256
TATACTA2509.094947E-1214.9634535
GTATTAA3300.014.6717341
TATATAC3450.014.0332963
CTATACA4900.013.9223784
GTACATG79750.013.7978691
GTCCTAC2805.820766E-1113.3617581
TCTATAC3800.013.31988053
GTATTAG3151.8189894E-1213.2744271
TACATGG80800.013.2639762
TGGACCG4150.013.2560715
Produced by FastQC (version 0.11.5)