##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2077118_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2617956 Sequences flagged as poor quality 0 Sequence length 50 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.003675768423914 31.0 30.0 34.0 23.0 34.0 2 29.04503971800901 31.0 30.0 34.0 22.0 34.0 3 29.147835945294727 31.0 30.0 34.0 22.0 34.0 4 32.5612038552214 35.0 33.0 37.0 24.0 37.0 5 32.477396105969696 35.0 33.0 37.0 24.0 37.0 6 32.62438100563951 36.0 33.0 37.0 25.0 37.0 7 32.39936118101297 35.0 33.0 37.0 23.0 37.0 8 32.356763444458196 35.0 33.0 37.0 22.0 37.0 9 33.74499456828151 38.0 34.0 39.0 22.0 39.0 10 33.65376499834222 38.0 34.0 39.0 22.0 39.0 11 33.70607489201499 38.0 34.0 39.0 22.0 39.0 12 33.60711791947611 38.0 34.0 39.0 21.0 39.0 13 33.54276045892291 38.0 34.0 39.0 19.0 39.0 14 34.46682297181465 39.0 34.0 41.0 18.0 41.0 15 34.507728930509145 39.0 34.0 41.0 18.0 41.0 16 34.46423316511049 39.0 34.0 41.0 18.0 41.0 17 34.29150719110634 39.0 33.0 41.0 17.0 41.0 18 34.25536601837464 39.0 33.0 41.0 17.0 41.0 19 34.374457019140124 39.0 34.0 41.0 18.0 41.0 20 34.374848545964866 39.0 34.0 41.0 18.0 41.0 21 34.31199225655435 39.0 34.0 41.0 18.0 41.0 22 34.22858672949431 39.0 34.0 41.0 18.0 41.0 23 34.17998698221055 39.0 34.0 41.0 18.0 41.0 24 34.19080496387258 39.0 34.0 41.0 18.0 41.0 25 34.088785678598114 39.0 34.0 41.0 17.0 41.0 26 34.037566712351165 39.0 34.0 41.0 16.0 41.0 27 33.98046071056962 39.0 33.0 41.0 16.0 41.0 28 33.86145680064906 39.0 33.0 41.0 15.0 41.0 29 33.84495614135608 39.0 33.0 41.0 15.0 41.0 30 33.69067279969564 38.0 33.0 40.0 10.0 41.0 31 33.6884523651276 38.0 33.0 40.0 10.0 41.0 32 33.56326500521781 38.0 33.0 40.0 9.0 41.0 33 33.51819205517587 38.0 33.0 40.0 9.0 41.0 34 33.43887788794006 38.0 33.0 40.0 9.0 41.0 35 33.3686131470506 38.0 33.0 40.0 8.0 41.0 36 33.271443064742115 38.0 33.0 40.0 8.0 41.0 37 33.17454304044835 38.0 33.0 40.0 8.0 41.0 38 33.08224889952314 38.0 32.0 40.0 5.0 41.0 39 32.96983142573825 38.0 32.0 40.0 2.0 41.0 40 32.894989831761876 38.0 32.0 40.0 2.0 41.0 41 32.79030969198871 38.0 32.0 40.0 2.0 41.0 42 32.63668640725818 38.0 32.0 40.0 2.0 41.0 43 32.540247429674146 38.0 32.0 40.0 2.0 41.0 44 32.38372837435006 38.0 31.0 40.0 2.0 41.0 45 32.23555285115563 38.0 31.0 40.0 2.0 41.0 46 32.09493093084834 38.0 31.0 40.0 2.0 41.0 47 31.93125743900967 38.0 31.0 40.0 2.0 41.0 48 31.764585424659543 38.0 31.0 40.0 2.0 41.0 49 31.57477971363919 38.0 31.0 40.0 2.0 41.0 50 31.35859617197539 37.0 31.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.9284010998004604 1101 2 2.064882114829299 1101 3 2.1366841810003017 1101 4 2.4096328218866105 1101 5 2.267857758713056 1101 6 2.4855759132477715 1101 7 2.334567347809724 1101 8 2.4340282643576714 1101 9 2.8999096472165107 1101 10 2.9149980336101393 1101 11 2.722489062079063 1101 12 2.943675233407376 1101 13 2.8853772265198003 1101 14 3.4100368915251096 1101 15 3.3483475234688598 1101 16 3.341108573151722 1101 17 3.4230121667374576 1101 18 3.4361669211122745 1101 19 3.3997779744820065 1101 20 3.4871150126208406 1101 21 3.386039872911141 1101 22 3.4241357578133247 1101 23 3.459972435221026 1101 24 3.4271351583591994 1101 25 3.3850889537444004 1101 26 3.4194755826831695 1101 27 3.514980385967206 1101 28 3.532833661794534 1101 29 3.5616275438794815 1101 30 3.6215147430839423 1101 31 3.714346812144555 1101 32 3.8116606454100435 1101 33 3.857072925623754 1101 34 3.885467531263629 1101 35 3.890484954094866 1101 36 3.8455756050324936 1101 37 3.7943314038847546 1101 38 3.853531534851463 1101 39 3.917539711413866 1101 40 3.8697210432143763 1101 41 3.947191261985189 1101 42 4.010679412307397 1101 43 3.896462538548832 1101 44 3.981728683289411 1101 45 4.046511428172714 1101 46 3.9631740089290304 1101 47 3.9507060931242037 1101 48 3.863185315362024 1101 49 3.830828107604866 1101 50 4.0279762323152255 1102 1 1.0345507096965356 1102 2 0.9327826918280238 1102 3 0.9926393860377303 1102 4 1.1578478152496885 1102 5 1.045734212374704 1102 6 1.2212243140604642 1102 7 1.2548053948730171 1102 8 1.2341056981916623 1102 9 1.4403766636478679 1102 10 1.5246888560602656 1102 11 1.3967186519385848 1102 12 1.4977679068323653 1102 13 1.4367655488122537 1102 14 1.6635710861822446 1102 15 1.577249446810093 1102 16 1.7000078315149594 1102 17 1.9427995372208002 1102 18 1.8898457026741227 1102 19 1.7911502267019372 1102 20 1.8403091765013144 1102 21 1.761273149627364 1102 22 1.774826811033769 1102 23 1.6121007537292797 1102 24 1.7121857254964112 1102 25 1.7082461542706895 1102 26 1.875769761255654 1102 27 1.6647109534106548 1102 28 1.888997216342915 1102 29 1.738681609392735 1102 30 1.8685250278574372 1102 31 1.8311898741058386 1102 32 1.9931981444940732 1102 33 1.8223115505242689 1102 34 1.9307422627880584 1102 35 1.895680385207008 1102 36 1.8765243481362717 1102 37 1.9675677334772814 1102 38 1.9186579827711938 1102 39 1.9310494363961581 1102 40 1.8472375243351422 1102 41 1.9273289731737577 1102 42 1.960102067245245 1102 43 1.7692180419471342 1102 44 1.6659080104243884 1102 45 1.7003307864124722 1102 46 1.693072473631922 1102 47 1.666000405492433 1102 48 1.541646059069528 1102 49 1.4800499230928708 1102 50 1.5368455780321355 1103 1 0.35198926565523436 1103 2 0.4508656390381418 1103 3 0.28151301301590337 1103 4 0.4290163030644152 1103 5 0.4989252275488525 1103 6 0.5356081550591583 1103 7 0.3858979689914648 1103 8 0.5613530500180772 1103 9 0.6571405041458434 1103 10 0.6002938269218205 1103 11 0.5364647727233063 1103 12 0.5559166524541013 1103 13 0.5505212320762851 1103 14 0.6426573383199283 1103 15 0.7297875066508723 1103 16 0.5257382874610528 1103 17 0.6524379100221438 1103 18 0.48133776704571574 1103 19 0.6530920309151895 1103 20 0.6027167239177942 1103 21 0.4158287470052713 1103 22 0.39642353541311337 1103 23 0.5968017103451686 1103 24 0.5577010144750147 1103 25 0.47618038443006583 1103 26 0.596273479344255 1103 27 0.4579613168790928 1103 28 0.6079186355159152 1103 29 0.7056311072106922 1103 30 0.7932515093335866 1103 31 0.6513572120825373 1103 32 0.7119609080966214 1103 33 0.7252318736102197 1103 34 0.729329990119524 1103 35 0.752333381809585 1103 36 0.6313690247656965 1103 37 0.7071223131940556 1103 38 0.6266478960575199 1103 39 0.8455602740001709 1103 40 0.6324191084854007 1103 41 0.6441896736550561 1103 42 0.718416387523007 1103 43 0.7188414712551818 1103 44 0.7145587779126004 1103 45 0.6185228084254106 1103 46 0.5425421029947586 1103 47 0.7050560101934593 1103 48 0.43928349075979156 1103 49 0.4301773129100397 1103 50 0.4267828394580633 1104 1 0.38027810514643434 1104 2 0.379567089392733 1104 3 0.3288740210761887 1104 4 0.2952105905570903 1104 5 0.49991825760768904 1104 6 0.24131783475788637 1104 7 0.3377685197998659 1104 8 0.47533873263903814 1104 9 0.5157212810266216 1104 10 0.23198605616293833 1104 11 0.28539116140599674 1104 12 0.3349502044549624 1104 13 0.3735873013840276 1104 14 0.5360615259278632 1104 15 0.47332640258947833 1104 16 0.41429415184055784 1104 17 0.4861375836567845 1104 18 0.4045960727086637 1104 19 0.4461578582840531 1104 20 0.3053417728533816 1104 21 0.29314946173476386 1104 22 0.45962247539659984 1104 23 0.34425701453709223 1104 24 0.45895788999697373 1104 25 0.3756879757579483 1104 26 0.408423662278274 1104 27 0.6190316208978217 1104 28 0.3146942871464873 1104 29 0.3732034137689979 1104 30 0.41083539954160386 1104 31 0.46801148063193665 1104 32 0.5290770818073014 1104 33 0.5301709569403954 1104 34 0.5832722642213923 1104 35 0.4244318066313397 1104 36 0.4214685492987513 1104 37 0.4849405275181695 1104 38 0.4818488527186986 1104 39 0.47136774503699286 1104 40 0.42033091713827275 1104 41 0.5421575611005238 1104 42 0.5158697135793631 1104 43 0.6683006177728288 1104 44 0.6940351561222755 1104 45 0.7136564808614096 1104 46 0.5921037943793195 1104 47 0.5351052282475735 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46 -1.742749233973317 2216 47 -1.6530955613594678 2216 48 -1.6915608603327144 2216 49 -1.658187652841253 2216 50 -1.6964923496180049 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 135532.0 3 6907.0 4 12539.0 5 9925.0 6 8175.0 7 7349.0 8 6528.0 9 6002.0 10 5808.0 11 5797.0 12 5830.0 13 6277.0 14 6641.0 15 6931.0 16 7475.0 17 8062.0 18 8884.0 19 9977.0 20 11876.0 21 14567.0 22 18049.0 23 20586.0 24 23026.0 25 24956.0 26 28165.0 27 31839.0 28 36845.0 29 42290.0 30 50313.0 31 59748.0 32 73474.0 33 90424.0 34 115949.0 35 150954.0 36 200210.0 37 273893.0 38 423012.0 39 663141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.688063714424324 16.308949050709977 12.48984535114919 25.51314188371651 2 16.67414762908292 18.652409762005988 38.11368106172062 26.55976154719047 3 20.57237857373366 22.227120686780875 27.13670769141356 30.063793048071908 4 13.927193278956054 14.656591116610358 34.48718085976872 36.929034744664875 5 15.725047476999416 35.96862063784159 32.6409293601129 15.665402525046101 6 35.58462236398916 33.4523090188003 16.192541107379665 14.770527509830877 7 31.254091647554795 30.04722520848533 19.313379023477314 19.385304120482562 8 28.114445363842925 33.697861028991625 18.578720362935204 19.608973244230242 9 27.300787596586357 15.609753860301073 17.81156158308175 39.27789696003082 10 15.962679751300247 27.935272335400736 31.71242849117819 24.389619422120827 11 37.392398977807005 21.68422613440378 21.469454016410577 19.453920871378635 12 24.91745636815664 23.36767316581916 28.11509290099924 23.599777565024965 13 29.38909549230871 20.252680801983903 24.594053261007062 25.764170444700323 14 23.483434156826043 19.59517224326507 25.127616974423866 31.793776625485027 15 25.327472866806872 27.221016047746932 21.94747516747693 25.504035917969265 16 25.454920687698134 26.407203364729575 23.378182381031547 24.75969356654074 17 24.571739774323305 26.255123587132033 24.788664571051854 24.38447206749281 18 24.620178348876024 25.996567244665414 25.19593496338891 24.18731944306965 19 25.3784978876892 25.550876820155267 25.275070528209564 23.795554763945972 20 25.66293981614204 25.031057087705218 25.081856927163965 24.22414616898878 21 25.72184277052451 24.79876020152077 25.034165943845903 24.445231084108812 22 25.701982651086208 24.54090290656045 24.932096408000334 24.825018034353004 23 25.22305014576992 24.741099779395913 24.846089284267396 25.18976079056677 24 25.038732872195858 24.973136416545138 24.94247886559416 25.04565184566484 25 25.30589135705433 24.7618126476127 24.993252537084285 24.93904345824869 26 25.141635587116777 24.92767219141027 25.05294609666849 24.87774612480446 27 25.120651435565733 25.095039883303066 24.892441037046154 24.89186764408505 28 25.288999052951013 24.963247846581886 24.867281235315865 24.880471865151236 29 25.0837508931326 25.165905711958093 24.947692726450448 24.80265066845886 30 25.3209622445032 25.204863824236856 24.60074682210679 24.87342710915316 31 25.281994326863472 24.834953920097593 24.78714272501645 25.095909028022483 32 25.166206023621207 25.168651915897993 24.72537215779674 24.939769902684063 33 25.09609111620098 25.004977972709625 24.717306567063897 25.181624344025494 34 25.317502537574445 24.920739015054234 24.923528818285213 24.838229629086108 35 25.24191027133546 25.039232308006294 24.84744689974951 24.87141052090874 36 25.379163266749096 24.972587268797643 24.775949413048423 24.872300051404842 37 25.38263175737525 24.644217917695904 24.923645639326118 25.049504685602724 38 25.263099564717255 24.864045798317722 24.891179314324585 24.981675322640434 39 25.311210614257828 25.158987615781967 24.70885392859927 24.820947841360937 40 25.4174673570567 24.815439909619442 24.831797689425382 24.935295043898474 41 25.176424012237618 25.028383372521684 24.847555208573365 24.94763740666733 42 25.338305037595394 24.963593016737466 24.67650231587008 25.02159962979706 43 25.1036038669606 24.705046621262476 25.013078256696087 25.17827125508084 44 25.158835907709907 24.86754917541361 24.87805735478938 25.0955575620871 45 25.217464731473182 25.01728943002576 24.754490093634203 25.010755744866852 46 25.383310147569965 24.66060971234498 24.756816939196497 25.19926320088856 47 25.162285992452066 24.905555264737565 24.831313888945143 25.100844853865233 48 25.031435006619407 25.026850137832618 24.815334857802203 25.126379997745772 49 25.198926222499352 24.76557921095846 24.76966739031262 25.265827176229568 50 25.26186767291099 24.93377608694905 24.802722850679164 25.0016333894608 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1313.0 1 956.5 2 600.0 3 593.0 4 586.0 5 561.5 6 537.0 7 548.5 8 560.0 9 621.5 10 683.0 11 829.0 12 975.0 13 1323.5 14 1672.0 15 2166.0 16 2660.0 17 2712.5 18 2765.0 19 2777.5 20 2790.0 21 2954.5 22 3119.0 23 3730.5 24 4342.0 25 5481.0 26 6620.0 27 8498.5 28 10377.0 29 13563.0 30 16749.0 31 22448.0 32 28147.0 33 36522.5 34 44898.0 35 55732.5 36 66567.0 37 78892.0 38 91217.0 39 104046.0 40 116875.0 41 130190.5 42 143506.0 43 155814.5 44 168123.0 45 180965.5 46 193808.0 47 201801.5 48 209795.0 49 214590.5 50 219386.0 51 220822.0 52 222258.0 53 218849.0 54 215440.0 55 206461.0 56 197482.0 57 184594.0 58 171706.0 59 157645.5 60 143585.0 61 125895.5 62 108206.0 63 93020.5 64 77835.0 65 65473.0 66 53111.0 67 43867.5 68 34624.0 69 28414.0 70 22204.0 71 17640.0 72 13076.0 73 10901.0 74 8726.0 75 6850.0 76 4974.0 77 3826.5 78 2679.0 79 2099.0 80 1519.0 81 1159.0 82 799.0 83 626.5 84 454.0 85 343.0 86 232.0 87 197.5 88 163.0 89 117.0 90 71.0 91 54.0 92 37.0 93 35.5 94 34.0 95 24.0 96 14.0 97 15.5 98 17.0 99 28.5 100 40.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08246891849977617 2 0.09274411029062368 3 0.10023086713451257 4 0.10114761287049896 5 0.09469219497959477 6 0.055577710244175224 7 0.05137595895423758 8 0.05771678362814348 9 0.057869574584141215 10 0.04377460889335038 11 0.046945021230303335 12 0.055806896678171826 13 0.04946607200426592 14 0.05107037704224211 15 0.05068839965224779 16 0.05351503233820584 17 0.052751077558217174 18 0.05343863686020697 19 0.06214772135207773 20 0.06898511663297625 21 0.07819077173183965 22 0.0807882179838011 23 0.05744939945514745 24 0.0749057661778884 25 0.08243072076077673 26 0.07975687903081641 27 0.0744473933098952 28 0.09408103115560384 29 0.08262170945577389 30 0.07914571520682548 31 0.05401160294519847 32 0.05057380643524948 33 0.05458456903018996 34 0.049045896875272166 35 0.056914631109155386 36 0.05618887406816616 37 0.058709924842128745 38 0.04835833757328237 39 0.0534768345992064 40 0.05592148989517012 41 0.04228489707237249 42 0.03869430960642578 43 0.03884710056242351 44 0.03617325883246319 45 0.02864830424957486 46 0.026318242170609435 47 0.031207552762536877 48 0.024866728088630975 49 0.024981321305629276 50 0.02670021956060377 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 2617956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.41122664291074 #Duplication Level Percentage of deduplicated Percentage of total 1 78.39585858206102 48.14385839248529 2 11.948369991936495 14.675281151763315 3 4.097703110982768 7.549349233917696 4 1.8332797130392573 4.503358238292169 5 0.9972821847822024 3.062216113829851 6 0.6269479217686528 2.3100984550221964 7 0.41620244003383894 1.7891651661975383 8 0.30578128087169976 1.50227228342172 9 0.2130951948132216 1.177779357467097 >10 1.1023239742211632 11.939619753971984 >50 0.0489703797256168 2.031065484173436 >100 0.01393431767232375 1.2201720840078123 >500 2.5090809168259065E-4 0.09576428544991236 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.819773899943315E-5 5 0.0 0.0 0.0 0.0 7.63954779988663E-5 6 0.0 0.0 0.0 0.0 7.63954779988663E-5 7 0.0 0.0 0.0 0.0 7.63954779988663E-5 8 0.0 0.0 0.0 0.0 7.63954779988663E-5 9 0.0 0.0 0.0 0.0 7.63954779988663E-5 10 0.0 0.0 0.0 0.0 7.63954779988663E-5 11 0.0 0.0 0.0 0.0 7.63954779988663E-5 12 0.0 0.0 0.0 0.0 1.9098869499716573E-4 13 0.0 0.0 0.0 0.0 1.9098869499716573E-4 14 0.0 0.0 0.0 0.0 1.9098869499716573E-4 15 0.0 0.0 0.0 0.0 3.055819119954652E-4 16 0.0 0.0 0.0 0.0 3.437796509948983E-4 17 0.0 0.0 0.0 0.0 3.437796509948983E-4 18 0.0 0.0 0.0 0.0 4.5837286799319774E-4 19 0.0 0.0 0.0 0.0 4.5837286799319774E-4 20 0.0 0.0 0.0 0.0 4.965706069926309E-4 21 0.0 0.0 0.0 0.0 4.965706069926309E-4 22 0.0 0.0 0.0 0.0 4.965706069926309E-4 23 0.0 0.0 0.0 1.1459321699829943E-4 4.965706069926309E-4 24 7.63954779988663E-5 0.0 0.0 2.2918643399659887E-4 4.965706069926309E-4 25 7.63954779988663E-5 0.0 0.0 2.67384172996032E-4 4.965706069926309E-4 26 7.63954779988663E-5 0.0 0.0 4.965706069926309E-4 5.729660849914972E-4 27 7.63954779988663E-5 0.0 0.0 6.493615629903635E-4 5.729660849914972E-4 28 7.63954779988663E-5 0.0 0.0 0.001833491471972791 5.729660849914972E-4 29 7.63954779988663E-5 0.0 0.0 0.002941225902956352 5.729660849914972E-4 30 1.1459321699829943E-4 0.0 0.0 0.0045837286799319775 5.729660849914972E-4 31 1.1459321699829943E-4 0.0 0.0 0.011344728482831645 5.729660849914972E-4 32 1.1459321699829943E-4 0.0 0.0 0.017876541851734713 6.493615629903635E-4 33 1.1459321699829943E-4 0.0 0.0 0.0268530105166015 6.875593019897966E-4 34 1.1459321699829943E-4 0.0 0.0 0.03922907795241784 7.639547799886629E-4 35 1.1459321699829943E-4 0.0 0.0 0.052980263992213776 7.639547799886629E-4 36 1.1459321699829943E-4 0.0 0.0 0.07234651766492638 7.639547799886629E-4 37 1.1459321699829943E-4 0.0 0.0 0.09568533619358002 7.639547799886629E-4 38 1.1459321699829943E-4 0.0 0.0 0.12425724496515603 8.403502579875293E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGTCGT 55 1.5920229E-4 24.00508 23 GTATCAA 3225 0.0 22.178202 1 GGTATCA 1700 0.0 19.677385 1 AACGTCG 80 8.977746E-5 19.254442 22 TATCAAC 4190 0.0 16.912117 2 TCAACGC 4200 0.0 16.504755 4 CAACGCA 4270 0.0 16.181719 5 ATCAACG 4385 0.0 15.909106 3 GTATAAT 215 2.1464075E-10 15.354141 1 AACGCAG 4555 0.0 15.212325 6 TATACTA 250 9.094947E-12 14.963453 5 GTATTAA 330 0.0 14.671734 1 TATATAC 345 0.0 14.033296 3 CTATACA 490 0.0 13.922378 4 GTACATG 7975 0.0 13.797869 1 GTCCTAC 280 5.820766E-11 13.361758 1 TCTATAC 380 0.0 13.3198805 3 GTATTAG 315 1.8189894E-12 13.274427 1 TACATGG 8080 0.0 13.263976 2 TGGACCG 415 0.0 13.256071 5 >>END_MODULE