FastQCFastQC Report
Mon 6 Feb 2017
SRR2077118_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077118_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2617956
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC701.1303819E-625.1498263
GGTATCA18450.022.659941
GTATCAA34500.020.9197391
GTTAACG1303.5915946E-818.61556230
GCGCTAA800.001986518516.50331113
ATCAACG46400.015.3188993
TCAACGC46450.015.1602824
TCTACGC2051.8062565E-915.0285543
TATCAAC47200.015.012062
CAACGCA47500.014.778555
GTCCTAG4700.014.5132731
AACGCAG50600.013.8280696
GTGTAGC8200.013.41706851
GTACATG73850.012.9908491
GTATAGC3450.012.75593951
TACATGG74950.012.7128442
ACCGTAT1751.5964742E-512.573238
GTATTAA3001.8553692E-1012.468931
CCGTATG1609.645598E-512.377019
TTAGTAC1250.00266113112.3234153
Produced by FastQC (version 0.11.5)