FastQCFastQC Report
Mon 6 Feb 2017
SRR2077117_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077117_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160100
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42200.020.1302431
TGCGATA2600.017.7675513
GCACCGT5150.016.231736
GGTATCA23250.016.186291
GTATTAC3750.015.8455281
GCGATAC2950.015.66052614
TATCAAC53500.015.4256962
TCAACGC54300.015.1579014
CAACGCA54850.015.1243585
TACACCG2355.0931703E-1114.9818145
GTACATG96700.014.9069571
GTGTACG3400.014.8875451
ATCAACG55300.014.8837993
AACGCAG57250.014.5630456
TACATGG98150.014.4174332
CGATACT3400.014.2357515
CGTGCCG3400.014.23372110
GGACCGT2803.6379788E-1214.1417426
GTGTAGG8200.013.9560871
TATACCG1453.832201E-513.65798955
Produced by FastQC (version 0.11.5)