FastQCFastQC Report
Mon 6 Feb 2017
SRR2077117_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077117_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3160100
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAC2100.022.004914
TATACCG1351.1277734E-1021.1875555
GTATCAA46750.020.4732041
GGTATCA24100.019.9942531
TGCGATA2300.019.13530213
GTACCGT3150.016.763256
GTATTAG3750.016.4287661
TACCGAT3750.016.4282467
GTACCGA4050.016.2976046
GCACCGT5700.015.8258316
CGATACT3000.015.40343115
TATCAAC61850.015.2970222
CGTGCCG3600.015.28118110
TCAACGC63550.014.78418354
ATACCGA1351.9564675E-514.6678436
CAACGCA64050.014.6339565
ATCAACG64600.014.54365353
CGAATCG3950.014.47873637
AACGCAG67650.013.9198166
TACAACG4150.013.786201516
Produced by FastQC (version 0.11.5)