FastQCFastQC Report
Mon 6 Feb 2017
SRR2077116_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077116_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178621
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49100.018.4648651
GTATACG1004.9375294E-415.403821
GGTATCA28850.014.7211541
GGACCGT1809.080759E-814.6647626
GTACATG93200.014.4971571
TCAACGC61100.014.4422064
TATCAAC63050.014.2747542
CAACGCA62000.014.2671565
ATCAACG62750.014.0977423
GTATATG6800.013.9152151
TACATGG95250.013.7692932
AACGCAG65100.013.48212056
GTATATA4950.013.336641
ACATGGG99700.013.1328513
GTATAGG5200.013.1186381
GTGTAGC11700.012.9775771
ATACCGT2054.4335866E-712.8763766
TAAGGCG2756.2573235E-1012.8023685
CGCGAAG1557.18275E-512.773540531
GCACCGT4000.012.6483576
Produced by FastQC (version 0.11.5)