##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2077116_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3178621
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.888800206127122	31.0	30.0	34.0	22.0	34.0
2	28.894580071043386	31.0	30.0	34.0	18.0	34.0
3	29.027222182197878	31.0	30.0	34.0	22.0	34.0
4	32.40295524379912	35.0	33.0	37.0	22.0	37.0
5	32.379280197293106	35.0	33.0	37.0	22.0	37.0
6	32.39891795844802	35.0	33.0	37.0	23.0	37.0
7	32.25249754531918	35.0	33.0	37.0	20.0	37.0
8	32.215985170927894	36.0	33.0	37.0	19.0	37.0
9	33.57517269281239	38.0	34.0	39.0	19.0	39.0
10	33.50983775668757	38.0	34.0	39.0	18.0	39.0
11	33.53763345803102	38.0	34.0	39.0	19.0	39.0
12	33.43756773770764	38.0	34.0	39.0	18.0	39.0
13	33.380278428916185	38.0	33.0	39.0	17.0	39.0
14	34.300897779257106	39.0	34.0	41.0	17.0	41.0
15	34.33398571267226	39.0	34.0	41.0	17.0	41.0
16	34.302963454907015	39.0	34.0	41.0	17.0	41.0
17	34.13923018818538	39.0	33.0	41.0	17.0	41.0
18	34.11228517020431	39.0	33.0	41.0	17.0	41.0
19	34.23512019835017	39.0	34.0	41.0	18.0	41.0
20	34.21734047563393	39.0	34.0	41.0	18.0	41.0
21	34.15794301994481	39.0	34.0	41.0	17.0	41.0
22	34.07637683133661	39.0	34.0	41.0	16.0	41.0
23	34.03649884651237	39.0	34.0	41.0	16.0	41.0
24	34.03969677416716	39.0	34.0	41.0	16.0	41.0
25	33.943440567466205	39.0	33.0	41.0	15.0	41.0
26	33.899254739712596	39.0	33.0	41.0	13.0	41.0
27	33.83635230497754	39.0	33.0	41.0	11.0	41.0
28	33.72403441618236	39.0	33.0	41.0	10.0	41.0
29	33.702576683410825	39.0	33.0	41.0	10.0	41.0
30	33.55260504476627	39.0	33.0	41.0	9.0	41.0
31	33.55501804084224	38.0	33.0	41.0	9.0	41.0
32	33.42727270725261	38.0	33.0	40.0	9.0	41.0
33	33.38583020750193	38.0	33.0	40.0	8.0	41.0
34	33.29999864721211	38.0	33.0	40.0	8.0	41.0
35	33.236787588076716	38.0	33.0	40.0	8.0	41.0
36	33.1501257935438	38.0	33.0	40.0	2.0	41.0
37	33.0510447769646	38.0	32.0	40.0	2.0	41.0
38	32.967839198193175	38.0	32.0	40.0	2.0	41.0
39	32.856953062349994	38.0	32.0	40.0	2.0	41.0
40	32.7876302333622	38.0	32.0	40.0	2.0	41.0
41	32.68795210249979	38.0	32.0	40.0	2.0	41.0
42	32.54121897514677	38.0	32.0	40.0	2.0	41.0
43	32.44581659782654	38.0	32.0	40.0	2.0	41.0
44	32.29149086978284	38.0	31.0	40.0	2.0	41.0
45	32.1439004524289	38.0	31.0	40.0	2.0	41.0
46	32.007571836969554	38.0	31.0	40.0	2.0	41.0
47	31.851077873077664	38.0	31.0	40.0	2.0	41.0
48	31.692308394111787	38.0	31.0	40.0	2.0	41.0
49	31.5154823428147	38.0	31.0	40.0	2.0	41.0
50	31.301893179463672	37.0	31.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.9975608601951151
1101	2	1.947197457178401
1101	3	2.1605824595435763
1101	4	2.3725229963493604
1101	5	2.444751647254442
1101	6	2.255090898322649
1101	7	2.3032968567045913
1101	8	2.4818370942585517
1101	9	2.9349741113534904
1101	10	2.913630904075461
1101	11	2.904401045338851
1101	12	3.0323499027567067
1101	13	3.013539479930081
1101	14	3.4759278513067144
1101	15	3.3659177234283533
1101	16	3.43603410118925
1101	17	3.5202804660016866
1101	18	3.5054453148489273
1101	19	3.414565625563256
1101	20	3.495785776741066
1101	21	3.48167744394965
1101	22	3.5079757429478207
1101	23	3.496392259367603
1101	24	3.4702122829209756
1101	25	3.4593814178543028
1101	26	3.546700581091258
1101	27	3.5432285244884483
1101	28	3.6147011309374832
1101	29	3.649295138864595
1101	30	3.8210182738176854
1101	31	3.753084432168528
1101	32	3.8525864888395915
1101	33	3.695331313059228
1101	34	3.7844969744593797
1101	35	3.926897134473819
1101	36	3.788622920500103
1101	37	3.7821088138608303
1101	38	3.8018467273292487
1101	39	3.874949366175464
1101	40	3.8672898825479436
1101	41	3.9367777127977703
1101	42	4.0795657392859965
1101	43	3.9365180663830017
1101	44	3.899333208156797
1101	45	3.8703069556706424
1101	46	3.840380801187578
1101	47	3.846455337884926
1101	48	3.672814299852188
1101	49	3.7840527713495717
1101	50	3.870198448237609
1102	1	1.2622100281456454
1102	2	1.0750632394456332
1102	3	1.2348249668437994
1102	4	1.4258102442574767
1102	5	1.4439778113832418
1102	6	1.352716454379113
1102	7	1.4864798764972136
1102	8	1.6159770179876958
1102	9	1.9047972941932798
1102	10	1.855155404923508
1102	11	1.7674826597414608
1102	12	1.8742589488145853
1102	13	1.7373516647768739
1102	14	2.0877817437633297
1102	15	1.9821823494417714
1102	16	2.126717143481855
1102	17	2.3041879853332645
1102	18	2.2732200989922404
1102	19	2.271374407594031
1102	20	2.194393477627081
1102	21	2.0425089779659444
1102	22	2.1237015965768222
1102	23	1.9949705875450334
1102	24	2.094753712758248
1102	25	2.200458946259509
1102	26	2.2202131888559578
1102	27	2.069666007664594
1102	28	2.268638423886479
1102	29	2.125869790135276
1102	30	2.2474295751923137
1102	31	2.2923941980623113
1102	32	2.3150477581824234
1102	33	2.229293200538031
1102	34	2.2706648832616168
1102	35	2.307174137933906
1102	36	2.3428924768740274
1102	37	2.429191547535453
1102	38	2.3056943885170966
1102	39	2.374493884647137
1102	40	2.3994681347981484
1102	41	2.3763164058185993
1102	42	2.369163054996797
1102	43	2.1439922343539877
1102	44	2.1091045019387025
1102	45	2.202301117128904
1102	46	2.1905137965574895
1102	47	2.13841556118296
1102	48	2.07688704271499
1102	49	2.2462569681287192
1102	50	2.2013322102434003
1103	1	0.3788720787688611
1103	2	0.43354327460353304
1103	3	0.3496163555362912
1103	4	0.4428967561785129
1103	5	0.5584588929544267
1103	6	0.43302758396540497
1103	7	0.5179275189211481
1103	8	0.6673201875398433
1103	9	0.6754979204062295
1103	10	0.6048233718445601
1103	11	0.6418272517616685
1103	12	0.5592149246420419
1103	13	0.6477278037794036
1103	14	0.8215810910432282
1103	15	0.7776091468374347
1103	16	0.6225011681399479
1103	17	0.835173461889795
1103	18	0.5692108794570174
1103	19	0.6711348027339596
1103	20	0.6975079939854609
1103	21	0.5534271015764887
1103	22	0.5733881432792103
1103	23	0.628183953294716
1103	24	0.6087324465882631
1103	25	0.6068116460008284
1103	26	0.7100765060752536
1103	27	0.6227147515970586
1103	28	0.7192832413909116
1103	29	0.7213000062106971
1103	30	0.8342560147661544
1103	31	0.6862836795774072
1103	32	0.8457087078916601
1103	33	0.7911301810411473
1103	34	0.8885542894666756
1103	35	0.7704675255631415
1103	36	0.8511872344524676
1103	37	0.9275565763242071
1103	38	0.8143527152566747
1103	39	0.8632685753185498
1103	40	0.7091487458797232
1103	41	0.6486740512499836
1103	42	0.7699975596821176
1103	43	0.7369812158004692
1103	44	0.7235818180075384
1103	45	0.6837523516438964
1103	46	0.6486398744944708
1103	47	0.6496100033014969
1103	48	0.6172321708238222
1103	49	0.5042277686398364
1103	50	0.528532415562367
1104	1	0.4832404872397902
1104	2	0.5035693468708438
1104	3	0.48953010134418307
1104	4	0.39101076726166895
1104	5	0.3914744141398643
1104	6	0.37721200655963116
1104	7	0.3734149539391396
1104	8	0.4643742233029968
1104	9	0.4543824879217979
1104	10	0.31632063085446305
1104	11	0.4362911123113733
1104	12	0.4040562942067041
1104	13	0.39549657956982287
1104	14	0.6230073511752181
1104	15	0.6043049699439962
1104	16	0.5389047778965974
1104	17	0.6875724200586149
1104	18	0.4879929690062923
1104	19	0.6029466937009218
1104	20	0.5564391320103752
1104	21	0.5833265241094594
1104	22	0.5485661826460344
1104	23	0.5536847547160519
1104	24	0.5504324382115016
1104	25	0.6107874225815806
1104	26	0.5897053723413634
1104	27	0.6959192764407902
1104	28	0.5826433456071385
1104	29	0.6437124549443993
1104	30	0.6164374849645711
1104	31	0.6275068495108513
1104	32	0.6554684834605737
1104	33	0.6527281847222923
1104	34	0.5794739258289852
1104	35	0.6569496534203836
1104	36	0.6332925522414428
1104	37	0.7033133077833256
1104	38	0.7055463181894055
1104	39	0.7649287888962633
1104	40	0.778425875755012
1104	41	0.8620768173703723
1104	42	0.7028070886000037
1104	43	0.7862217776198364
1104	44	0.7491559078455268
1104	45	0.8103961676212634
1104	46	0.6933739519361737
1104	47	0.6998966260712649
1104	48	0.7771940317105894
1104	49	0.7803803270793566
1104	50	0.8079883330846727
1105	1	0.4076820342413754
1105	2	0.46732856601930095
1105	3	0.38070793818747006
1105	4	0.5073887079215709
1105	5	0.39056789312274276
1105	6	0.42235739927453153
1105	7	0.4674448045663624
1105	8	0.5369787362311058
1105	9	0.5074221628424311
1105	10	0.5397212918479113
1105	11	0.5547893334867098
1105	12	0.4242963071215726
1105	13	0.5607195364419013
1105	14	0.6677620357644543
1105	15	0.47231445700887065
1105	16	0.3945850846118475
1105	17	0.5841978775264991
1105	18	0.4859079826310264
1105	19	0.4071787522338397
1105	20	0.5748930620308315
1105	21	0.5818815780229514
1105	22	0.4799078736317597
1105	23	0.4795107891414361
1105	24	0.4698395652509433
1105	25	0.5046701464947816
1105	26	0.563798755611316
1105	27	0.6579220837008037
1105	28	0.662880804582187
1105	29	0.607323837507991
1105	30	0.7008663723369466
1105	31	0.544551741742886
1105	32	0.7079690154649043
1105	33	0.6891109397421928
1105	34	0.6773762016589515
1105	35	0.6894896941238997
1105	36	0.691658172733959
1105	37	0.6621322645571368
1105	38	0.6230655477131393
1105	39	0.7178429363190446
1105	40	0.7582016953527386
1105	41	0.6429572757586968
1105	42	0.772913733533457
1105	43	0.7154344941663311
1105	44	0.6298926213764275
1105	45	0.6566209548434614
1105	46	0.7706173112938473
1105	47	0.7042000784682827
1105	48	0.7512128386617682
1105	49	0.6298156846452869
1105	50	0.7670491245093061
1106	1	0.23766635591892182
1106	2	0.17870682279530925
1106	3	0.15347656977458257
1106	4	0.13878282001178377
1106	5	0.14185283355558198
1106	6	0.21373293745728006
1106	7	0.21796093217253087
1106	8	0.3333629442563506
1106	9	0.33723075137885417
1106	10	0.4315435808043233
1106	11	0.2890925448958015
1106	12	0.3508469338021527
1106	13	0.371203025196273
1106	14	0.4578480120631312
1106	15	0.4289497872208017
1106	16	0.33616588995892016
1106	17	0.47466794951966307
1106	18	0.15850254873260639
1106	19	0.2937944159092396
1106	20	0.2920272084826152
1106	21	0.2485162026277763
1106	22	0.3105379398730861
1106	23	0.2292149965971646
1106	24	0.20519249505829862
1106	25	0.42489788059842937
1106	26	0.3926535159460016
1106	27	0.41445872331343026
1106	28	0.3633621120115862
1106	29	0.30782465336129405
1106	30	0.42260434950375014
1106	31	0.3628874891125733
1106	32	0.48161741403962566
1106	33	0.37473996487872085
1106	34	0.4322654020707972
1106	35	0.3457524055729948
1106	36	0.40975063117846133
1106	37	0.34265177388567025
1106	38	0.4081959910255506
1106	39	0.44916871386149637
1106	40	0.4490909939360108
1106	41	0.48243606416286156
1106	42	0.334027529237936
1106	43	0.4257697188990477
1106	44	0.5223487767836801
1106	45	0.5919020338669725
1106	46	0.43654703675919393
1106	47	0.43870781367635203
1106	48	0.4573610285828842
1106	49	0.4753918388065159
1106	50	0.5514231050859948
1107	1	-0.1732724779005146
1107	2	-0.1926927106423868
1107	3	-0.1813492467345128
1107	4	-0.2711050571189446
1107	5	-0.21720167020353642
1107	6	-0.2304918212854048
1107	7	-0.1325387340123143
1107	8	-0.1671665250627683
1107	9	-0.19917198987711515
1107	10	-0.31302690046558723
1107	11	-0.23593904164507506
1107	12	-0.42179105116201043
1107	13	-0.3340560846952698
1107	14	-0.46810024161355557
1107	15	-0.3324980057778646
1107	16	-0.36103299330849836
1107	17	-0.2846267993523952
1107	18	-0.2981231518955454
1107	19	-0.3907493370121671
1107	20	-0.3030356125450311
1107	21	-0.3370784838462555
1107	22	-0.5719245756194198
1107	23	-0.5621391176196795
1107	24	-0.6157090996889494
1107	25	-0.5608625913530432
1107	26	-0.39033574651954694
1107	27	-0.34349235882684326
1107	28	-0.4002354860617032
1107	29	-0.39482732816345845
1107	30	-0.42272798764115294
1107	31	-0.469357766968038
1107	32	-0.387058462280109
1107	33	-0.3596366799523665
1107	34	-0.46740168508137003
1107	35	-0.3554566554127234
1107	36	-0.5202656789906897
1107	37	-0.4306147414041632
1107	38	-0.4532659766815641
1107	39	-0.3426618494782758
1107	40	-0.34436225732797254
1107	41	-0.34810946082846783
1107	42	-0.4071272560333554
1107	43	-0.4516582301247496
1107	44	-0.32569884992822296
1107	45	-0.22730499685011551
1107	46	-0.2497588761224918
1107	47	-0.28288633665745877
1107	48	-0.3315350307787597
1107	49	-0.3080242073181978
1107	50	-0.28050221749080606
1108	1	-0.14140603396328189
1108	2	-0.2215377336393729
1108	3	-0.16359974131533406
1108	4	-0.22919040459123607
1108	5	-0.17512617555703258
1108	6	-0.27287037756531163
1108	7	-0.3737378921778749
1108	8	-0.20972068245483655
1108	9	-0.2736655361788607
1108	10	-0.24764583484772373
1108	11	-0.38913136554671013
1108	12	-0.5502591634682119
1108	13	-0.40784525610683886
1108	14	-0.5244654168182663
1108	15	-0.4195647462549488
1108	16	-0.4123160907374839
1108	17	-0.6493630978679477
1108	18	-0.5455747914651639
1108	19	-0.42911147665512317
1108	20	-0.6464426156642062
1108	21	-0.4466484778700277
1108	22	-0.5320279256636411
1108	23	-0.7067711480970758
1108	24	-0.5147297284344319
1108	25	-0.4143618868622454
1108	26	-0.5225943701375542
1108	27	-0.497738559854227
1108	28	-0.619106798612215
1108	29	-0.7714893955877997
1108	30	-0.5241966465710135
1108	31	-0.5932835479312857
1108	32	-0.3388532331455778
1108	33	-0.4725979583810087
1108	34	-0.34043531966816687
1108	35	-0.2735776238314145
1108	36	-0.5782239754844909
1108	37	-0.5335702723253348
1108	38	-0.3943901498881601
1108	39	-0.41445133183324145
1108	40	-0.389225347187363
1108	41	-0.26952616655623984
1108	42	-0.1436883812157319
1108	43	-0.2942846679482045
1108	44	-0.24747378965203382
1108	45	-0.30830154108204155
1108	46	-0.1958900957825236
1108	47	-0.26851038166945074
1108	48	-0.20068573648273258
1108	49	-0.2589934404579033
1108	50	-0.21512729501415961
1109	1	-0.5261315654137242
1109	2	-0.44929778479113835
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2210	39	-0.13666867088232948
2210	40	-0.33904006128673814
2210	41	-0.23671765225556385
2210	42	-0.1996458829599419
2210	43	-0.43984427653745684
2210	44	-0.31560031144716305
2210	45	-0.12320638940153117
2210	46	-0.12655193665334252
2210	47	-0.1564609383206701
2210	48	-0.13181132620218605
2210	49	0.0679885632179058
2210	50	-0.0920554617061562
2211	1	-0.33155217623708566
2211	2	-0.36181734790924835
2211	3	-0.4183105712154891
2211	4	-0.39422562881326684
2211	5	-0.6123371177715775
2211	6	-0.4883503971383689
2211	7	-0.3948452574522072
2211	8	-0.3264713420742318
2211	9	-0.3070216966536634
2211	10	-0.4558997892250076
2211	11	-0.3969543665630084
2211	12	-0.5565578760417225
2211	13	-0.4168631911976206
2211	14	-0.7257106495276133
2211	15	-0.5519374272662887
2211	16	-0.47790921393969654
2211	17	-0.44051600207530583
2211	18	-0.6353141431500617
2211	19	-0.4708848419950229
2211	20	-0.6161137834060497
2211	21	-0.562471329538532
2211	22	-0.5976743223143615
2211	23	-0.7221917624628773
2211	24	-0.640330003331492
2211	25	-0.5627994722363567
2211	26	-0.6579124202139894
2211	27	-0.6092465657455719
2211	28	-0.39262224518030564
2211	29	-0.3431499244246723
2211	30	-0.8456938642095935
2211	31	-0.6837310873961329
2211	32	-0.38916158168688497
2211	33	-0.5271234088224332
2211	34	-0.507059337220781
2211	35	-0.4239271340799533
2211	36	-0.4622897200803493
2211	37	-0.282376552460498
2211	38	-0.3727691739307275
2211	39	-0.2694763688362869
2211	40	-0.38802115476427446
2211	41	-0.48197475995507233
2211	42	-0.40522686376936434
2211	43	-0.4473122693620297
2211	44	-0.4449923093847623
2211	45	-0.4440776517499678
2211	46	-0.41398081788514673
2211	47	-0.49890787748346455
2211	48	-0.43713029991972263
2211	49	-0.33592546050207517
2211	50	-0.35456606909952626
2212	1	-0.443940220303638
2212	2	-0.518900137374537
2212	3	-0.4932489542088696
2212	4	-0.4004205090015134
2212	5	-0.43540394614620226
2212	6	-0.37868477574391335
2212	7	-0.316318861936864
2212	8	-0.2781832206849586
2212	9	-0.5066514027829285
2212	10	-0.4649914233089092
2212	11	-0.5150093775518272
2212	12	-0.6729141470683686
2212	13	-0.4366452908542655
2212	14	-1.3416914777536917
2212	15	-0.9127396231369076
2212	16	-0.8292337909358736
2212	17	-0.630337366760692
2212	18	-0.6357458468865573
2212	19	-0.7363590776674585
2212	20	-0.652769947044959
2212	21	-0.7404723492633707
2212	22	-0.8561879224431479
2212	23	-0.9093687152019143
2212	24	-0.779340825849367
2212	25	-0.5400532979060202
2212	26	-0.6916255191802634
2212	27	-0.7421780630809991
2212	28	-0.8035386940285036
2212	29	-0.7246245187467011
2212	30	-0.7620627412368748
2212	31	-0.6230463255947996
2212	32	-0.5253964208257358
2212	33	-0.5948862476345909
2212	34	-0.5900374116571427
2212	35	-0.6550344824609837
2212	36	-0.8122956235947285
2212	37	-0.7560792440783715
2212	38	-0.8444216889314262
2212	39	-0.7618169288208705
2212	40	-0.676479517824788
2212	41	-0.70227309161303
2212	42	-0.5884575612747653
2212	43	-0.6806346562875341
2212	44	-0.5697399304460156
2212	45	-0.5505324789160753
2212	46	-0.5268799450231718
2212	47	-0.6105223244336528
2212	48	-0.6554353817063294
2212	49	-0.5945620505138045
2212	50	-0.6010410865807323
2213	1	-0.6646377653467717
2213	2	-0.6444770423771935
2213	3	-0.6591157465724322
2213	4	-0.9471229249462141
2213	5	-0.7277222403294594
2213	6	-0.8046496391574394
2213	7	-0.9086743282294094
2213	8	-0.9150970681055135
2213	9	-0.9162198805404316
2213	10	-1.0194439357281126
2213	11	-1.0653722968504482
2213	12	-1.025992220654743
2213	13	-0.8985002095918375
2213	14	-1.0188227855027279
2213	15	-1.2491617563825912
2213	16	-1.0431013052191886
2213	17	-1.008996837147933
2213	18	-0.9932815251883085
2213	19	-1.0594996283839535
2213	20	-1.0356604122134527
2213	21	-1.1648363255118568
2213	22	-1.0020342596791991
2213	23	-0.9854074765924139
2213	24	-1.1619043777239213
2213	25	-1.1245989852049547
2213	26	-1.10191231737398
2213	27	-1.1660955406310975
2213	28	-1.2414238276198333
2213	29	-1.214463927962484
2213	30	-1.3189992951931941
2213	31	-1.1807736637474662
2213	32	-1.243026155839388
2213	33	-1.3377197084681498
2213	34	-1.2383077957257242
2213	35	-1.4012575431032346
2213	36	-1.527270753613557
2213	37	-1.334484499989113
2213	38	-1.2601039337639541
2213	39	-1.4381872752692644
2213	40	-1.2001726148124092
2213	41	-1.3002122340277502
2213	42	-1.2639129471593478
2213	43	-1.3093720353170362
2213	44	-1.1215876039094006
2213	45	-1.1809996960796099
2213	46	-1.1385557262205204
2213	47	-1.0932283687816948
2213	48	-1.252218446180013
2213	49	-1.320558172876261
2213	50	-1.1354882389588
2214	1	-0.7313967252593585
2214	2	-0.8831514720552178
2214	3	-0.872461963219461
2214	4	-0.8966217147946303
2214	5	-0.7283369568063165
2214	6	-0.6589154802312862
2214	7	-0.7076089316527572
2214	8	-0.8098157207728001
2214	9	-0.9188703092569241
2214	10	-0.82215011065162
2214	11	-0.9516828075122277
2214	12	-1.0029401025973002
2214	13	-0.9155843956209111
2214	14	-0.9289192141465321
2214	15	-0.8143535288028332
2214	16	-0.824588146408864
2214	17	-0.6345803576570717
2214	18	-0.9190045827637618
2214	19	-0.8945884597396656
2214	20	-0.8247117327834843
2214	21	-0.8560657759778465
2214	22	-0.9000946826634646
2214	23	-0.9965857383307579
2214	24	-1.173873479357603
2214	25	-1.0322711793521435
2214	26	-1.0280801509119044
2214	27	-1.09249224883866
2214	28	-0.962684786951371
2214	29	-0.8665633151795049
2214	30	-0.9099523341066416
2214	31	-0.7766395381875526
2214	32	-0.9657216561397988
2214	33	-0.922809388632956
2214	34	-0.9974552325778134
2214	35	-1.061823827784366
2214	36	-0.9538419233607556
2214	37	-0.9687606118316339
2214	38	-0.9204206864426823
2214	39	-0.8317158261155555
2214	40	-0.804359891903971
2214	41	-0.758369859412305
2214	42	-0.7353094210956002
2214	43	-0.8300897854232794
2214	44	-0.6964116976073456
2214	45	-0.6323581640975
2214	46	-0.7162004035853258
2214	47	-0.7186929845397607
2214	48	-0.7397979031988058
2214	49	-0.706144050751611
2214	50	-0.732400382286091
2215	1	-0.39021866035537656
2215	2	-0.4464259092733478
2215	3	-0.45438611375544724
2215	4	-0.42341499800033233
2215	5	-0.4368235264262239
2215	6	-0.4150764655104737
2215	7	-0.44170227334319634
2215	8	-0.3508519925652571
2215	9	-0.40185886504989554
2215	10	-0.39127650409682246
2215	11	-0.47652882773589766
2215	12	-0.45187730862567577
2215	13	-0.490666493776196
2215	14	-0.4439518732824155
2215	15	-0.4223395867516899
2215	16	-0.5094936603540106
2215	17	-0.4335880591789092
2215	18	-0.3092567355535749
2215	19	-0.4150856629985995
2215	20	-0.4845153557973134
2215	21	-0.458331735724137
2215	22	-0.3782922679025731
2215	23	-0.588351992752294
2215	24	-0.4962477034076116
2215	25	-0.7044571081730169
2215	26	-0.6263523393667185
2215	27	-0.6191099793383046
2215	28	-0.5176477710124487
2215	29	-0.605142714537358
2215	30	-0.6463832357171952
2215	31	-0.591320613451046
2215	32	-0.5966161108977204
2215	33	-0.4589247667967058
2215	34	-0.6137111950372542
2215	35	-0.5894029820016868
2215	36	-0.643360111043279
2215	37	-0.5415179910963985
2215	38	-0.5744177365943273
2215	39	-0.5401725975106118
2215	40	-0.6378369626800691
2215	41	-0.7283503818571617
2215	42	-0.6638221076106774
2215	43	-0.5804593608094812
2215	44	-0.6753174040623939
2215	45	-0.6568943461082952
2215	46	-0.6790271356678339
2215	47	-0.7915305059016049
2215	48	-0.8369263253156056
2215	49	-0.9744355872958472
2215	50	-0.940440189618581
2216	1	-0.522239537656155
2216	2	-0.5772598047221074
2216	3	-0.5786322668823694
2216	4	-0.505724557747083
2216	5	-0.5039480388884918
2216	6	-0.6763726201957319
2216	7	-0.5936611342858598
2216	8	-0.7037074276092028
2216	9	-0.6588794640757101
2216	10	-0.6895143557250663
2216	11	-0.8913093467075726
2216	12	-0.801393583258573
2216	13	-0.8131576780253837
2216	14	-0.8719988728104653
2216	15	-0.9294115275080586
2216	16	-1.0271375452060596
2216	17	-0.915731154843165
2216	18	-0.905017689569199
2216	19	-0.9691153905562828
2216	20	-1.0286057973862839
2216	21	-1.138257091248228
2216	22	-0.9371381002341153
2216	23	-1.017506944043042
2216	24	-1.1903725954369264
2216	25	-1.1634812591004504
2216	26	-1.1450361377531664
2216	27	-1.1068241397493566
2216	28	-1.198316120686087
2216	29	-1.425675260463855
2216	30	-1.2107739749490705
2216	31	-1.3724250756457224
2216	32	-1.2477777140731732
2216	33	-1.193913780501191
2216	34	-1.212771377006817
2216	35	-1.2607429983865117
2216	36	-1.2494388049764353
2216	37	-1.4926547164902146
2216	38	-1.3178519779945148
2216	39	-1.3546057736742476
2216	40	-1.3890139266334458
2216	41	-1.4540189931092655
2216	42	-1.3761937671980284
2216	43	-1.5901445817375404
2216	44	-1.5993824280163018
2216	45	-1.666093698952043
2216	46	-1.811683036432754
2216	47	-1.5817338636786111
2216	48	-1.7761571955815896
2216	49	-1.8793913304458307
2216	50	-1.7785250679345062
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	177963.0
3	8652.0
4	15699.0
5	12458.0
6	10308.0
7	9077.0
8	7825.0
9	7122.0
10	7014.0
11	6898.0
12	7067.0
13	7503.0
14	7969.0
15	8358.0
16	9077.0
17	9611.0
18	10605.0
19	11949.0
20	14372.0
21	17869.0
22	21901.0
23	25239.0
24	27720.0
25	30622.0
26	34495.0
27	38728.0
28	44218.0
29	51539.0
30	59542.0
31	71930.0
32	87612.0
33	108302.0
34	137856.0
35	178767.0
36	236902.0
37	324897.0
38	505594.0
39	825361.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.36032841971627	16.853330797597938	12.56760211792681	25.218738664758988
2	16.285698000996966	18.96214077634286	38.59835188763941	26.153809335020767
3	20.15839179547127	22.933680836249223	27.719320013717883	29.18860735456162
4	13.354628583377737	15.125233202198432	35.67127591362984	35.84886230079399
5	14.996742392013207	36.58899945617313	33.36688034517734	15.047377806636325
6	34.52906215974689	34.4456150611468	16.677206414000224	14.348116365106081
7	30.397711802868056	30.616282850576916	20.086030873790016	18.89997447276502
8	27.431598934517933	34.14283276955456	19.109896882942405	19.315671412985104
9	27.016209379360802	15.701217064910233	18.280180852011544	39.002392703717426
10	15.410794313441794	28.341611464264503	32.54927929745468	23.69831492483902
11	36.508795823152504	22.166462656741157	22.151952719637485	19.17278880046885
12	24.413411549323794	23.738150189307376	28.93717790360496	22.91126035776387
13	28.943509313866976	20.750025888902144	24.96628147547542	25.34018332175546
14	22.955524576071927	20.131803048964546	25.838389707564936	31.074282667398588
15	24.78058192531413	27.949947120590235	22.564368091053307	24.705102863042327
16	24.823516783965406	27.01056459834979	24.068039249199934	24.097879368484868
17	23.756601915251853	26.877487276194323	25.405495072427094	23.96041573612673
18	23.777707828386163	26.647832792974285	25.87201359816173	23.702445780477824
19	24.64861291464998	26.270617400543028	25.86773698500767	23.213032699799317
20	24.971926251119566	25.660146295162374	25.668677818511515	23.69924963520654
21	25.084111796431507	25.444298564605354	25.63157049616987	23.840019142793274
22	25.023165516236013	25.146494537094267	25.432604011829763	24.397735934839957
23	24.593868554685777	25.377989954684015	25.472613356064777	24.55552813456543
24	24.350627066530066	25.469599378883945	25.59078123730802	24.58899231727797
25	24.566864922560555	25.361977059410645	25.673466560454404	24.397691457574393
26	24.44886145208584	25.651969533772007	25.689909061627965	24.209259952514195
27	24.41809139771405	25.691124689573186	25.6315580025892	24.259225910123565
28	24.53964528195654	25.697896713396222	25.48121659541089	24.281241409236348
29	24.364996676644605	25.84254379791984	25.60668159736424	24.185777928071317
30	24.66592950027801	25.746554135446768	25.43400356283095	24.153512801444275
31	24.58309908986576	25.30534540969891	25.4788167310587	24.63273876937663
32	24.362775741253806	25.8104074873575	25.46781819978835	24.358998571600342
33	24.61135696674134	25.554859206512536	25.273483129868957	24.560300696877164
34	24.689342412192943	25.514604581418425	25.50173100778049	24.294321998608144
35	24.70337223734754	25.61281141075292	25.413113161606688	24.270703190292853
36	24.71866680013271	25.621891956719228	25.226372890276433	24.43306835287163
37	24.673998070351885	25.25604038095598	25.648108362490536	24.4218531862016
38	24.71903251372811	25.506962730929057	25.429375227018202	24.34462952832463
39	24.73188578465056	25.664489814162252	25.299040483035252	24.30458391815194
40	24.800737086274285	25.358493820732786	25.490260268638607	24.350508824354318
41	24.525573175587105	25.67373408807288	25.414138934394625	24.38655380194539
42	24.781598574434618	25.510274733540506	25.36868124501559	24.33944544700929
43	24.541892478803558	25.292847656274585	25.460782647556822	24.70447721736503
44	24.55219173960716	25.599375101495653	25.386816454317827	24.46161670457936
45	24.613122735820795	25.473101739846282	25.228998361105585	24.684777163227338
46	24.659026613892443	25.386665172977395	25.24090459668187	24.713403616448286
47	24.615425767936884	25.49607102059056	25.377556511532035	24.510946699940522
48	24.499990244940115	25.611972668210274	25.328289233120127	24.559747853729487
49	24.731460255173726	25.362267078057858	25.38313033873691	24.523142328031504
50	24.687008012154333	25.411849781468103	25.404486189646473	24.496656016731087
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1797.0
1	1347.0
2	897.0
3	902.0
4	907.0
5	873.5
6	840.0
7	817.5
8	795.0
9	850.0
10	905.0
11	1073.5
12	1242.0
13	1803.0
14	2364.0
15	2971.0
16	3578.0
17	3783.0
18	3988.0
19	4187.5
20	4387.0
21	4812.0
22	5237.0
23	6468.5
24	7700.0
25	10120.0
26	12540.0
27	16744.5
28	20949.0
29	27417.5
30	33886.0
31	43978.0
32	54070.0
33	66607.0
34	79144.0
35	93031.5
36	106919.0
37	120333.0
38	133747.0
39	147058.5
40	160370.0
41	173218.5
42	186067.0
43	196817.5
44	207568.0
45	219748.0
46	231928.0
47	241581.0
48	251234.0
49	252487.5
50	253741.0
51	252804.5
52	251868.0
53	246417.5
54	240967.0
55	229337.0
56	217707.0
57	203864.0
58	190021.0
59	173596.0
60	157171.0
61	137889.5
62	118608.0
63	101969.0
64	85330.0
65	71711.5
66	58093.0
67	47945.0
68	37797.0
69	30456.0
70	23115.0
71	18049.0
72	12983.0
73	10647.0
74	8311.0
75	6365.0
76	4419.0
77	3404.0
78	2389.0
79	1837.5
80	1286.0
81	1013.0
82	740.0
83	583.5
84	427.0
85	333.5
86	240.0
87	179.5
88	119.0
89	94.0
90	69.0
91	57.5
92	46.0
93	40.0
94	34.0
95	26.0
96	18.0
97	21.5
98	25.0
99	31.5
100	38.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08447059275075575
2	0.09400302835726561
3	0.10079842799754989
4	0.1027174991922598
5	0.09368842652206727
6	0.055307002627869126
7	0.051028417669171634
8	0.05798111822705506
9	0.059145145017288944
10	0.04322629215625266
11	0.0468127530775138
12	0.05590474611474599
13	0.049832930695417915
14	0.05058797509989395
15	0.049738550144858416
16	0.05316771014852038
17	0.05354523235075839
18	0.054142975837635256
19	0.06279452630558975
20	0.06814275750396162
21	0.07836731714790784
22	0.08066391054485578
23	0.05716315345553937
24	0.07559882099816241
25	0.08151333549989132
26	0.07909090136886404
27	0.07389997108809135
28	0.09349966542094827
29	0.0822369197208475
30	0.07849315788198719
31	0.05433173693875425
32	0.05156324078900881
33	0.05455195822339311
34	0.049392488126140235
35	0.057509215474257545
36	0.05609350721586499
37	0.05901930428320961
38	0.049172266841501396
39	0.053796913818917075
40	0.05625080813346417
41	0.04297461068809399
42	0.038224122976598975
43	0.03778368040732129
44	0.036021910130210555
45	0.02733889947873622
46	0.02538836810050648
47	0.029918634527362653
48	0.024633323696030447
49	0.024727704246589953
50	0.025923191220343665
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3178621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.9300445241344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.27624387973519	43.60756159562659
2	13.099754923775281	15.177387719791016
3	4.8517929390512515	8.4319374294336
4	2.315741502533493	5.366040333926047
5	1.2775551952843724	3.7004414672431456
6	0.7844724191336647	2.7266713301021173
7	0.49625893115184794	2.012381138398813
8	0.3506068441694777	1.6248536074563276
9	0.26201117880344843	1.366048732851424
>10	1.212397800353325	12.130091126777716
>50	0.05720875179522436	2.2384496688503357
>100	0.015462717043117807	1.375293873975545
>500	3.8338002141369623E-4	0.15616255605425675
>1k	1.0953714887967499E-4	0.0866794195130886
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	3.146018351983454E-5	0.0	0.0	0.0	0.0
13	3.146018351983454E-5	0.0	0.0	0.0	0.0
14	3.146018351983454E-5	0.0	0.0	0.0	0.0
15	3.146018351983454E-5	0.0	0.0	0.0	0.0
16	3.146018351983454E-5	0.0	0.0	3.146018351983454E-5	0.0
17	3.146018351983454E-5	0.0	0.0	3.146018351983454E-5	0.0
18	6.292036703966909E-5	0.0	0.0	3.146018351983454E-5	0.0
19	9.438055055950363E-5	0.0	0.0	3.146018351983454E-5	0.0
20	9.438055055950363E-5	0.0	0.0	3.146018351983454E-5	0.0
21	9.438055055950363E-5	0.0	0.0	9.438055055950363E-5	0.0
22	9.438055055950363E-5	0.0	0.0	1.2584073407933817E-4	0.0
23	9.438055055950363E-5	0.0	0.0	1.5730091759917273E-4	0.0
24	1.2584073407933817E-4	0.0	0.0	2.202212846388418E-4	0.0
25	1.2584073407933817E-4	0.0	0.0	2.5168146815867634E-4	0.0
26	1.2584073407933817E-4	0.0	0.0	5.977434868768563E-4	0.0
27	1.2584073407933817E-4	0.0	0.0	8.494249550355327E-4	0.0
28	1.5730091759917273E-4	0.0	0.0	0.0018246906441504035	0.0
29	1.5730091759917273E-4	0.0	0.0	0.0031145581684636197	0.0
30	1.5730091759917273E-4	0.0	0.0	0.005348231198371873	0.0
31	1.5730091759917273E-4	0.0	0.0	0.013055976160731337	0.0
32	1.5730091759917273E-4	0.0	0.0	0.021361464609967656	0.0
33	1.5730091759917273E-4	0.0	0.0	0.0320579270067114	0.0
34	1.5730091759917273E-4	0.0	0.0	0.046623991976394795	3.146018351983454E-5
35	2.202212846388418E-4	0.0	0.0	0.06326642905838727	3.146018351983454E-5
36	2.202212846388418E-4	0.0	0.0	0.08421891128259708	3.146018351983454E-5
37	2.202212846388418E-4	0.0	0.0	0.11199825333061098	3.146018351983454E-5
38	2.202212846388418E-4	0.0	0.0	0.1470763579552265	3.146018351983454E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4910	0.0	18.464865	1
GTATACG	100	4.9375294E-4	15.40382	1
GGTATCA	2885	0.0	14.721154	1
GGACCGT	180	9.080759E-8	14.664762	6
GTACATG	9320	0.0	14.497157	1
TCAACGC	6110	0.0	14.442206	4
TATCAAC	6305	0.0	14.274754	2
CAACGCA	6200	0.0	14.267156	5
ATCAACG	6275	0.0	14.097742	3
GTATATG	680	0.0	13.915215	1
TACATGG	9525	0.0	13.769293	2
AACGCAG	6510	0.0	13.4821205	6
GTATATA	495	0.0	13.33664	1
ACATGGG	9970	0.0	13.132851	3
GTATAGG	520	0.0	13.118638	1
GTGTAGC	1170	0.0	12.977577	1
ATACCGT	205	4.4335866E-7	12.876376	6
TAAGGCG	275	6.2573235E-10	12.802368	5
CGCGAAG	155	7.18275E-5	12.7735405	31
GCACCGT	400	0.0	12.648357	6
>>END_MODULE