FastQCFastQC Report
Mon 6 Feb 2017
SRR2077116_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2077116_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3178621
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53450.019.1824761
CGAATCG2500.017.59595937
GGTATCA30850.016.974161
TACCGAT3700.015.460297
TATCAAC69050.014.5619332
ACCGATC4750.014.3591178
GTACCGA3850.014.2855826
TCTACAC8700.014.1623473
TCAACGC70450.014.0539374
ATCGTTA1100.001019075713.99920929
CAACGCA70800.013.9223085
TTACGCG950.006254894713.89067443
ATCAACG71600.013.8896693
GCACCGT4600.013.8694366
CGTATAC1150.001423675813.3926543
GTAATAC3151.8189894E-1213.271183
AACGCAG74350.013.2265086
TGCACCG3051.8189894E-1112.9849175
CCAGTAC8500.012.9424823
GTACATG88600.012.8884331
Produced by FastQC (version 0.11.5)